Mutagenetix > Incidental Mutation

Incidental Mutation 'R9109:Or10a5'

692140

Institutional Source

Beutler Lab

Gene Symbol

Or10a5

Ensembl Gene

ENSMUSG00000073898

Gene Name

olfactory receptor family 10 subfamily A member 5

Synonyms

MOR263-1, Olfr713, P3, GA_x6K02T2PBJ9-9415724-9416677

MMRRC Submission

068918-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R9109 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

106635343-106636317 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 106635640 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 93 (I93F)

Ref Sequence

ENSEMBL: ENSMUSP00000150042 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098139] [ENSMUST00000213623]

AlphaFold

Q920G5

Predicted Effect

probably damaging
Transcript: ENSMUST00000098139
AA Change: I100F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000095743
Gene: ENSMUSG00000073898
AA Change: I100F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	39	316	4.4e-57	PFAM
Pfam:7tm_1	49	298	2e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213623
AA Change: I93F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (61/62)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a reporter allele exhibit abnormal olfactory sensory neuron projections. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700030K09Rik	C	A	8: 73,198,923 (GRCm39)	A110D	probably benign	Het
4930407I10Rik	A	G	15: 81,947,615 (GRCm39)	E504G	probably benign	Het
4931406B18Rik	C	A	7: 43,148,702 (GRCm39)	A170S		Het
Apbb2	T	C	5: 66,609,018 (GRCm39)	K210E	probably benign	Het
Arid4a	C	A	12: 71,122,129 (GRCm39)	L515I	probably damaging	Het
Atp13a3	A	G	16: 30,134,716 (GRCm39)	S1186P	possibly damaging	Het
Brf1	A	G	12: 112,927,011 (GRCm39)	Y500H	probably damaging	Het
Btaf1	A	G	19: 36,964,114 (GRCm39)	Y895C	probably benign	Het
Cdhr17	T	A	5: 16,997,909 (GRCm39)	V47E	possibly damaging	Het
Cenpf	T	C	1: 189,391,571 (GRCm39)	T737A	probably benign	Het
Cmklr1	G	C	5: 113,752,043 (GRCm39)	H319Q	probably benign	Het
Col18a1	G	A	10: 76,893,204 (GRCm39)	T1057M	probably damaging	Het
Col1a2	T	A	6: 4,515,260 (GRCm39)		probably null	Het
Csmd3	A	C	15: 47,617,187 (GRCm39)	H1000Q		Het
Cyp4a29	T	A	4: 115,108,395 (GRCm39)	M373K	probably damaging	Het
Dnhd1	A	G	7: 105,333,173 (GRCm39)	E215G	probably damaging	Het
Dusp19	T	C	2: 80,447,729 (GRCm39)	M1T	probably null	Het
Etv4	A	G	11: 101,664,492 (GRCm39)	Y253H	probably benign	Het
Exosc6	G	A	8: 111,783,513 (GRCm39)	A171T	probably damaging	Het
Fam219a	G	A	4: 41,521,870 (GRCm39)	T70M	probably damaging	Het
Fau	G	A	19: 6,108,297 (GRCm39)	R6H	probably benign	Het
Flrt3	T	C	2: 140,501,879 (GRCm39)	D583G	probably damaging	Het
Gm14443	A	T	2: 175,011,869 (GRCm39)	C192*	probably null	Het
Gm4353	T	A	7: 115,682,843 (GRCm39)	N246I	probably benign	Het
Igfn1	A	G	1: 135,926,327 (GRCm39)	L41P	probably benign	Het
Jakmip3	A	T	7: 138,622,560 (GRCm39)	I307F	probably damaging	Het
Kcnt2	T	A	1: 140,353,035 (GRCm39)	V248D	probably damaging	Het
Kctd14	A	G	7: 97,107,243 (GRCm39)	K166R	probably benign	Het
Kdm5b	T	C	1: 134,528,493 (GRCm39)		probably null	Het
Kif5c	A	G	2: 49,620,151 (GRCm39)	D461G	probably damaging	Het
Lpin2	T	C	17: 71,538,516 (GRCm39)		probably null	Het
Mettl22	G	A	16: 8,305,231 (GRCm39)	D295N	probably damaging	Het
Nox4	G	A	7: 87,025,448 (GRCm39)	R525Q	probably benign	Het
Or5b95	T	A	19: 12,658,190 (GRCm39)	C239*	probably null	Het
Or5k8	A	T	16: 58,644,677 (GRCm39)	Y132N	probably damaging	Het
Pecam1	G	T	11: 106,586,587 (GRCm39)	N309K	probably damaging	Het
Pigc	C	T	1: 161,798,032 (GRCm39)	R5C	probably benign	Het
Pih1d1	T	C	7: 44,809,193 (GRCm39)	V259A	possibly damaging	Het
Ptpre	A	G	7: 135,271,508 (GRCm39)	I374M	probably benign	Het
Rnps1	A	G	17: 24,637,573 (GRCm39)	S72G	unknown	Het
S100pbp	A	G	4: 129,044,847 (GRCm39)	Y363H	probably damaging	Het
Sema5a	A	G	15: 32,619,040 (GRCm39)	T523A	probably benign	Het
Slc38a8	A	G	8: 120,212,851 (GRCm39)	F270S	possibly damaging	Het
Slc4a1ap	T	C	5: 31,693,538 (GRCm39)	S491P	probably damaging	Het
Slc66a2	T	C	18: 80,300,300 (GRCm39)	L57P	probably damaging	Het
Slc7a6	T	C	8: 106,922,534 (GRCm39)	V440A	probably damaging	Het
Sobp	A	T	10: 42,898,902 (GRCm39)	C228S	probably damaging	Het
Sox8	T	C	17: 25,787,813 (GRCm39)	D212G	possibly damaging	Het
Specc1	A	G	11: 62,102,464 (GRCm39)		probably null	Het
Svop	G	A	5: 114,168,231 (GRCm39)	T456M	probably benign	Het
Synrg	A	G	11: 83,900,278 (GRCm39)	D750G	probably damaging	Het
Syt14	C	A	1: 192,612,944 (GRCm39)	E336*	probably null	Het
Tcp10c	A	T	17: 13,576,650 (GRCm39)	Q76H	probably damaging	Het
Tcp11l1	C	T	2: 104,528,897 (GRCm39)	V134I	possibly damaging	Het
Tex51	T	C	18: 32,594,029 (GRCm39)	R110G	possibly damaging	Het
Tle3	T	A	9: 61,319,562 (GRCm39)	H443Q	possibly damaging	Het
Unc13a	C	T	8: 72,108,335 (GRCm39)	R506Q	possibly damaging	Het
Vmn2r27	T	C	6: 124,174,224 (GRCm39)	Y542C	possibly damaging	Het
Vmn2r5	T	G	3: 64,411,411 (GRCm39)	N386H	possibly damaging	Het
Vmn2r91	A	G	17: 18,327,905 (GRCm39)	I500V	probably damaging	Het
Wdfy4	A	T	14: 32,760,704 (GRCm39)		probably null	Het
Zfp709	A	T	8: 72,644,648 (GRCm39)	E692D	possibly damaging	Het
Zglp1	A	T	9: 20,977,482 (GRCm39)	L111Q	probably benign	Het

Other mutations in Or10a5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01324:Or10a5	APN	7	106,636,054 (GRCm39)	missense	probably damaging	0.99
IGL01630:Or10a5	APN	7	106,636,318 (GRCm39)	utr 3 prime	probably benign
IGL02539:Or10a5	APN	7	106,635,641 (GRCm39)	missense	probably damaging	1.00
IGL02727:Or10a5	APN	7	106,635,902 (GRCm39)	missense	probably damaging	1.00
IGL03336:Or10a5	APN	7	106,635,542 (GRCm39)	missense	probably damaging	1.00
R0501:Or10a5	UTSW	7	106,635,439 (GRCm39)	missense	probably benign
R0684:Or10a5	UTSW	7	106,635,889 (GRCm39)	missense	probably damaging	1.00
R0909:Or10a5	UTSW	7	106,635,401 (GRCm39)	missense	probably benign	0.19
R1481:Or10a5	UTSW	7	106,635,356 (GRCm39)	missense	probably benign	0.05
R1958:Or10a5	UTSW	7	106,635,478 (GRCm39)	missense	possibly damaging	0.77
R1965:Or10a5	UTSW	7	106,635,565 (GRCm39)	missense	probably damaging	1.00
R2119:Or10a5	UTSW	7	106,635,938 (GRCm39)	missense	probably damaging	1.00
R2149:Or10a5	UTSW	7	106,635,545 (GRCm39)	missense	possibly damaging	0.68
R3012:Or10a5	UTSW	7	106,635,569 (GRCm39)	missense	possibly damaging	0.79
R3428:Or10a5	UTSW	7	106,635,923 (GRCm39)	missense	probably benign
R4425:Or10a5	UTSW	7	106,635,698 (GRCm39)	missense	probably damaging	1.00
R4795:Or10a5	UTSW	7	106,636,121 (GRCm39)	missense	probably benign	0.00
R4796:Or10a5	UTSW	7	106,636,121 (GRCm39)	missense	probably benign	0.00
R4908:Or10a5	UTSW	7	106,635,364 (GRCm39)	start codon destroyed	probably benign	0.02
R4945:Or10a5	UTSW	7	106,635,526 (GRCm39)	missense	probably benign	0.00
R5122:Or10a5	UTSW	7	106,636,055 (GRCm39)	nonsense	probably null
R5721:Or10a5	UTSW	7	106,635,565 (GRCm39)	missense	probably damaging	1.00
R5979:Or10a5	UTSW	7	106,635,543 (GRCm39)	missense	probably damaging	1.00
R6739:Or10a5	UTSW	7	106,636,018 (GRCm39)	missense	probably damaging	1.00
R6981:Or10a5	UTSW	7	106,635,956 (GRCm39)	missense	possibly damaging	0.77
R7197:Or10a5	UTSW	7	106,635,364 (GRCm39)	missense	probably benign	0.03
R7228:Or10a5	UTSW	7	106,636,307 (GRCm39)	missense	probably benign
R7444:Or10a5	UTSW	7	106,635,554 (GRCm39)	missense	probably damaging	1.00
R8830:Or10a5	UTSW	7	106,635,889 (GRCm39)	missense	probably benign	0.28
R9298:Or10a5	UTSW	7	106,635,640 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGTTACTCTGCTGGGAAAC -3'
(R):5'- ACTTTGTTGGTGGCACAGAATG -3'

Sequencing Primer
(F):5'- GGAAACAGCCTCATCATTCTGGTG -3'
(R):5'- ACTGAAGAGCCATGTGGTC -3'

Posted On

2021-12-30