Mutagenetix > Incidental Mutation

Incidental Mutation 'R9112:Map2'

692278

Institutional Source

Beutler Lab

Gene Symbol

Map2

Ensembl Gene

ENSMUSG00000015222

Gene Name

microtubule-associated protein 2

Synonyms

G1-397-34, MAP-2, Mtap2, repro4

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.751) question?

Stock #

R9112 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66214432-66481742 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 66472723 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 1709 (C1709*)

Ref Sequence

ENSEMBL: ENSMUSP00000109646 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024639] [ENSMUST00000077355] [ENSMUST00000114012] [ENSMUST00000114013] [ENSMUST00000114015] [ENSMUST00000114017] [ENSMUST00000114018] [ENSMUST00000172886] [ENSMUST00000173778] [ENSMUST00000173800]

AlphaFold

P20357

Predicted Effect

probably null
Transcript: ENSMUST00000024639
AA Change: C348*

SMART Domains

Protein: ENSMUSP00000024639
Gene: ENSMUSG00000015222
AA Change: C348*

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000077355
AA Change: C348*

SMART Domains

Protein: ENSMUSP00000076577
Gene: ENSMUSG00000015222
AA Change: C348*

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000114012
AA Change: C347*

SMART Domains

Protein: ENSMUSP00000109645
Gene: ENSMUSG00000015222
AA Change: C347*

Domain	Start	End	E-Value	Type
low complexity region	133	140	N/A	INTRINSIC
low complexity region	181	195	N/A	INTRINSIC
low complexity region	205	221	N/A	INTRINSIC
low complexity region	228	250	N/A	INTRINSIC
Pfam:Tubulin-binding	299	330	2.1e-18	PFAM
Pfam:Tubulin-binding	331	361	9.1e-20	PFAM
Pfam:Tubulin-binding	362	393	1.7e-17	PFAM
low complexity region	421	434	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000114013
AA Change: C1709*

SMART Domains

Protein: ENSMUSP00000109646
Gene: ENSMUSG00000015222
AA Change: C1709*

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	86	103	1.2e-5	PFAM
low complexity region	120	141	N/A	INTRINSIC
Pfam:MAP2_projctn	376	1510	N/A	PFAM
low complexity region	1543	1557	N/A	INTRINSIC
low complexity region	1567	1583	N/A	INTRINSIC
low complexity region	1590	1612	N/A	INTRINSIC
Pfam:Tubulin-binding	1662	1692	1.7e-13	PFAM
Pfam:Tubulin-binding	1693	1723	5.8e-18	PFAM
Pfam:Tubulin-binding	1724	1755	5.9e-18	PFAM
low complexity region	1783	1796	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000114015
AA Change: C348*

SMART Domains

Protein: ENSMUSP00000109648
Gene: ENSMUSG00000015222
AA Change: C348*

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000114017
AA Change: C379*

SMART Domains

Protein: ENSMUSP00000109650
Gene: ENSMUSG00000015222
AA Change: C379*

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000114018
AA Change: C379*

SMART Domains

Protein: ENSMUSP00000109651
Gene: ENSMUSG00000015222
AA Change: C379*

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000172486

SMART Domains

Protein: ENSMUSP00000134187
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
Pfam:Tubulin-binding	12	41	2.5e-17	PFAM
low complexity region	65	81	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000172886
AA Change: C309*

SMART Domains

Protein: ENSMUSP00000133446
Gene: ENSMUSG00000015222
AA Change: C309*

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	107	3.9e-54	PFAM
low complexity region	112	126	N/A	INTRINSIC
low complexity region	136	152	N/A	INTRINSIC
low complexity region	159	181	N/A	INTRINSIC
Pfam:Tubulin-binding	230	261	1.4e-18	PFAM
Pfam:Tubulin-binding	262	292	1.4e-19	PFAM
Pfam:Tubulin-binding	293	323	6e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000173778
AA Change: C354*

SMART Domains

Protein: ENSMUSP00000134651
Gene: ENSMUSG00000015222
AA Change: C354*

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	124	4.8e-84	PFAM
low complexity region	157	171	N/A	INTRINSIC
low complexity region	181	197	N/A	INTRINSIC
low complexity region	204	226	N/A	INTRINSIC
Pfam:Tubulin-binding	275	306	1.6e-18	PFAM
Pfam:Tubulin-binding	307	337	1.6e-19	PFAM
Pfam:Tubulin-binding	338	368	7e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000173800
AA Change: C221*

SMART Domains

Protein: ENSMUSP00000134518
Gene: ENSMUSG00000015222
AA Change: C221*

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	23	2.2e-11	PFAM
low complexity region	55	69	N/A	INTRINSIC
low complexity region	79	95	N/A	INTRINSIC
low complexity region	102	124	N/A	INTRINSIC
Pfam:Tubulin-binding	173	204	8.7e-19	PFAM
Pfam:Tubulin-binding	205	235	3.8e-20	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (56/56)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit altered contextual memory. Mice homozygous for another knock-out allele display decreased body weight, altered microtubule density and organization in Purkinje cell dendrites, and reduced dendritic length inhippocampal neurons. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310033P09Rik	A	G	11: 59,100,806 (GRCm39)	S181G	possibly damaging	Het
5730596B20Rik	A	G	6: 52,156,413 (GRCm39)	R160G	unknown	Het
Acy1	A	G	9: 106,311,952 (GRCm39)	Y262H	probably benign	Het
Adamts13	T	C	2: 26,880,379 (GRCm39)	S681P	possibly damaging	Het
Adgra3	C	A	5: 50,118,395 (GRCm39)	C1051F	probably damaging	Het
Afp	T	C	5: 90,652,289 (GRCm39)		probably null	Het
Agrn	A	T	4: 156,261,514 (GRCm39)	V568E	probably damaging	Het
Ahnak	A	G	19: 8,987,149 (GRCm39)	E2811G	probably damaging	Het
Ap1m1	C	A	8: 72,994,066 (GRCm39)	Y7*	probably null	Het
Apobec1	T	A	6: 122,555,837 (GRCm39)	T207S	probably benign	Het
Arhgap32	G	A	9: 32,157,309 (GRCm39)	R102Q	probably damaging	Het
Bcat2	T	C	7: 45,237,446 (GRCm39)		probably null	Het
Cd209f	T	A	8: 4,155,802 (GRCm39)		probably benign	Het
Cox7c	G	A	13: 86,194,837 (GRCm39)		probably benign	Het
Cyp2d11	A	G	15: 82,276,203 (GRCm39)	V156A	possibly damaging	Het
Dhrs3	A	T	4: 144,653,769 (GRCm39)	T297S	probably benign	Het
Dip2c	T	C	13: 9,660,766 (GRCm39)	C876R	probably damaging	Het
Eprs1	A	T	1: 185,129,273 (GRCm39)	I587F	probably damaging	Het
Fam13c	A	G	10: 70,286,978 (GRCm39)	T81A	probably benign	Het
Flt4	A	G	11: 49,524,064 (GRCm39)	Y548C	probably damaging	Het
Garin2	T	C	12: 78,757,202 (GRCm39)		probably null	Het
Gimap4	T	A	6: 48,667,629 (GRCm39)	V128E	probably benign	Het
Gm32742	A	G	9: 51,060,735 (GRCm39)	L856P	possibly damaging	Het
Gm5916	A	G	9: 36,032,258 (GRCm39)	Y59H	probably damaging	Het
Gnai3	A	T	3: 108,030,990 (GRCm39)	H57Q		Het
Golga3	G	T	5: 110,333,757 (GRCm39)	R131L	probably benign	Het
Gpc6	A	T	14: 117,424,088 (GRCm39)	I59F	probably benign	Het
H2bc6	T	C	13: 23,769,753 (GRCm39)	M63V	possibly damaging	Het
Herc6	T	C	6: 57,596,604 (GRCm39)	S515P	probably damaging	Het
Ift74	T	C	4: 94,575,103 (GRCm39)	I518T	probably benign	Het
Kat6b	T	C	14: 21,675,256 (GRCm39)	S474P	possibly damaging	Het
Klhl3	A	C	13: 58,248,212 (GRCm39)	I28S	unknown	Het
Klrh1	T	A	6: 129,743,697 (GRCm39)	I196F	probably benign	Het
Lipi	A	G	16: 75,359,159 (GRCm39)	F259S	probably damaging	Het
Lrrc8a	T	A	2: 30,145,782 (GRCm39)	S199T	probably damaging	Het
Mrgprf	A	G	7: 144,861,503 (GRCm39)	S22G	probably benign	Het
Myo1e	G	T	9: 70,274,983 (GRCm39)	R712L	probably benign	Het
Nras	A	T	3: 102,967,658 (GRCm39)	N85Y	probably benign	Het
Or4a71	C	T	2: 89,358,337 (GRCm39)	C139Y	probably damaging	Het
Or5b3	A	T	19: 13,388,475 (GRCm39)	I181F	probably benign	Het
Or6ae1	A	G	7: 139,742,660 (GRCm39)	S68P		Het
Or9q2	T	C	19: 13,772,780 (GRCm39)	H65R	probably damaging	Het
Pcp2	T	A	8: 3,674,638 (GRCm39)		probably benign	Het
Pigv	T	C	4: 133,392,079 (GRCm39)	R364G	probably benign	Het
Plekhh3	T	A	11: 101,061,625 (GRCm39)	S30C	probably damaging	Het
Poglut3	A	G	9: 53,295,530 (GRCm39)	Y44C	probably damaging	Het
Pramel11	A	C	4: 143,623,334 (GRCm39)	V280G	possibly damaging	Het
Pramel51	C	T	12: 88,144,055 (GRCm39)	A261T	possibly damaging	Het
Rhof	T	C	5: 123,258,571 (GRCm39)		probably benign	Het
Rufy3	A	T	5: 88,780,336 (GRCm39)	E361D		Het
Serpina1b	T	C	12: 103,698,699 (GRCm39)	D50G	probably benign	Het
Sgsm2	G	A	11: 74,756,222 (GRCm39)	Q376*	probably null	Het
Slc18b1	A	T	10: 23,692,262 (GRCm39)	I246F	probably damaging	Het
Slc38a9	A	T	13: 112,850,777 (GRCm39)	N387I	probably damaging	Het
Synrg	C	G	11: 83,862,409 (GRCm39)	P35A	probably damaging	Het
Tdrp	T	C	8: 14,005,796 (GRCm39)	K42E	probably damaging	Het
Trappc10	A	C	10: 78,029,201 (GRCm39)	M1112R	probably damaging	Het
Ube2e1	A	G	14: 18,285,203 (GRCm38)	V90A	probably damaging	Het
Vwde	C	A	6: 13,205,051 (GRCm39)	V277F	possibly damaging	Het
Zfyve16	A	G	13: 92,659,563 (GRCm39)	M116T	possibly damaging	Het

Other mutations in Map2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Map2	APN	1	66,464,490 (GRCm39)	missense	probably damaging	1.00
IGL02135:Map2	APN	1	66,419,920 (GRCm39)	nonsense	probably null
IGL02526:Map2	APN	1	66,419,876 (GRCm39)	missense	possibly damaging	0.94
Annas	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
calliope	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
carthage	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
costas	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
Jacobin	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
Nectar	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
ruby_throat	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
Rufous	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
Speckled	UTSW	1	66,451,886 (GRCm39)	nonsense	probably null
Sunbird	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
swift	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
E0370:Map2	UTSW	1	66,455,883 (GRCm39)	unclassified	probably benign
PIT4362001:Map2	UTSW	1	66,451,677 (GRCm39)	missense	probably benign	0.05
R0067:Map2	UTSW	1	66,452,322 (GRCm39)	missense	probably benign	0.04
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0268:Map2	UTSW	1	66,419,881 (GRCm39)	nonsense	probably null
R0302:Map2	UTSW	1	66,453,987 (GRCm39)	missense	probably benign	0.15
R0305:Map2	UTSW	1	66,452,253 (GRCm39)	missense	probably benign	0.00
R0409:Map2	UTSW	1	66,472,739 (GRCm39)	missense	probably damaging	1.00
R0561:Map2	UTSW	1	66,464,656 (GRCm39)	missense	probably damaging	1.00
R0674:Map2	UTSW	1	66,452,361 (GRCm39)	missense	probably damaging	1.00
R0738:Map2	UTSW	1	66,464,348 (GRCm39)	splice site	probably benign
R0893:Map2	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
R1305:Map2	UTSW	1	66,464,554 (GRCm39)	missense	probably damaging	1.00
R1534:Map2	UTSW	1	66,452,339 (GRCm39)	missense	probably benign	0.33
R1632:Map2	UTSW	1	66,454,245 (GRCm39)	missense	possibly damaging	0.60
R1682:Map2	UTSW	1	66,454,781 (GRCm39)	splice site	probably null
R1774:Map2	UTSW	1	66,453,233 (GRCm39)	missense	probably damaging	1.00
R2014:Map2	UTSW	1	66,455,295 (GRCm39)	missense	possibly damaging	0.55
R2017:Map2	UTSW	1	66,451,958 (GRCm39)	missense	probably damaging	1.00
R2050:Map2	UTSW	1	66,453,473 (GRCm39)	missense	probably damaging	0.98
R2093:Map2	UTSW	1	66,438,599 (GRCm39)	missense	probably damaging	1.00
R2214:Map2	UTSW	1	66,459,345 (GRCm39)	missense	probably damaging	0.99
R2284:Map2	UTSW	1	66,453,227 (GRCm39)	missense	probably damaging	1.00
R3011:Map2	UTSW	1	66,453,771 (GRCm39)	missense	probably damaging	1.00
R3105:Map2	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
R3708:Map2	UTSW	1	66,455,714 (GRCm39)	unclassified	probably benign
R3709:Map2	UTSW	1	66,455,015 (GRCm39)	nonsense	probably null
R3729:Map2	UTSW	1	66,451,605 (GRCm39)	missense	possibly damaging	0.80
R3760:Map2	UTSW	1	66,478,077 (GRCm39)	missense	probably damaging	1.00
R3788:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R3789:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R4003:Map2	UTSW	1	66,454,899 (GRCm39)	missense	probably damaging	1.00
R4120:Map2	UTSW	1	66,455,063 (GRCm39)	missense	probably damaging	1.00
R4172:Map2	UTSW	1	66,452,759 (GRCm39)	missense	possibly damaging	0.89
R4198:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4200:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4205:Map2	UTSW	1	66,464,449 (GRCm39)	missense	probably damaging	1.00
R4613:Map2	UTSW	1	66,464,628 (GRCm39)	missense	probably damaging	1.00
R4700:Map2	UTSW	1	66,449,796 (GRCm39)	missense	probably damaging	0.96
R4974:Map2	UTSW	1	66,452,664 (GRCm39)	missense	probably benign	0.15
R5007:Map2	UTSW	1	66,452,448 (GRCm39)	missense	possibly damaging	0.86
R5039:Map2	UTSW	1	66,477,955 (GRCm39)	missense	probably damaging	1.00
R5237:Map2	UTSW	1	66,478,169 (GRCm39)	unclassified	probably benign
R5313:Map2	UTSW	1	66,464,538 (GRCm39)	missense	probably damaging	1.00
R5455:Map2	UTSW	1	66,438,550 (GRCm39)	missense	probably damaging	1.00
R5490:Map2	UTSW	1	66,452,292 (GRCm39)	missense	probably damaging	1.00
R5517:Map2	UTSW	1	66,454,415 (GRCm39)	missense	probably benign	0.00
R5532:Map2	UTSW	1	66,453,779 (GRCm39)	missense	probably damaging	1.00
R5583:Map2	UTSW	1	66,455,196 (GRCm39)	missense	probably damaging	1.00
R5764:Map2	UTSW	1	66,454,034 (GRCm39)	missense	probably damaging	0.99
R5996:Map2	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
R6058:Map2	UTSW	1	66,454,573 (GRCm39)	missense	probably benign	0.05
R6199:Map2	UTSW	1	66,464,637 (GRCm39)	missense	probably damaging	1.00
R6208:Map2	UTSW	1	66,470,749 (GRCm39)	missense	probably damaging	1.00
R6276:Map2	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
R6378:Map2	UTSW	1	66,454,488 (GRCm39)	missense	probably damaging	1.00
R6424:Map2	UTSW	1	66,453,946 (GRCm39)	missense	possibly damaging	0.67
R6743:Map2	UTSW	1	66,454,766 (GRCm39)	missense	probably benign	0.04
R6837:Map2	UTSW	1	66,453,731 (GRCm39)	missense	probably damaging	1.00
R6901:Map2	UTSW	1	66,460,932 (GRCm39)	missense	possibly damaging	0.94
R6984:Map2	UTSW	1	66,454,395 (GRCm39)	missense	possibly damaging	0.90
R6989:Map2	UTSW	1	66,454,065 (GRCm39)	missense	probably benign	0.00
R7001:Map2	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
R7055:Map2	UTSW	1	66,455,983 (GRCm39)	missense	probably damaging	1.00
R7094:Map2	UTSW	1	66,451,886 (GRCm39)	nonsense	probably null
R7106:Map2	UTSW	1	66,449,903 (GRCm39)	missense	possibly damaging	0.92
R7182:Map2	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
R7235:Map2	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
R7424:Map2	UTSW	1	66,453,983 (GRCm39)	missense	possibly damaging	0.69
R7473:Map2	UTSW	1	66,454,617 (GRCm39)	missense	probably damaging	1.00
R7642:Map2	UTSW	1	66,452,466 (GRCm39)	missense	probably benign	0.01
R7660:Map2	UTSW	1	66,453,536 (GRCm39)	missense	probably damaging	1.00
R7673:Map2	UTSW	1	66,452,933 (GRCm39)	missense	probably benign	0.03
R7768:Map2	UTSW	1	66,453,642 (GRCm39)	missense	possibly damaging	0.94
R7796:Map2	UTSW	1	66,455,654 (GRCm39)	splice site	probably null
R7834:Map2	UTSW	1	66,455,647 (GRCm39)	missense	probably damaging	1.00
R7842:Map2	UTSW	1	66,455,570 (GRCm39)	missense	probably benign
R7955:Map2	UTSW	1	66,452,875 (GRCm39)	missense	probably damaging	1.00
R8056:Map2	UTSW	1	66,454,779 (GRCm39)	missense	probably damaging	0.99
R8118:Map2	UTSW	1	66,464,550 (GRCm39)	missense	probably damaging	1.00
R8135:Map2	UTSW	1	66,452,828 (GRCm39)	missense	probably damaging	1.00
R8152:Map2	UTSW	1	66,453,902 (GRCm39)	missense	probably benign	0.00
R8223:Map2	UTSW	1	66,464,649 (GRCm39)	missense	probably damaging	1.00
R8329:Map2	UTSW	1	66,454,272 (GRCm39)	missense	probably benign	0.01
R8344:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8475:Map2	UTSW	1	66,453,164 (GRCm39)	missense	probably damaging	1.00
R8548:Map2	UTSW	1	66,452,499 (GRCm39)	missense	probably damaging	1.00
R8680:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8713:Map2	UTSW	1	66,453,781 (GRCm39)	missense	probably damaging	1.00
R8745:Map2	UTSW	1	66,452,556 (GRCm39)	missense	probably benign	0.00
R8786:Map2	UTSW	1	66,472,755 (GRCm39)	critical splice donor site	probably benign
R8790:Map2	UTSW	1	66,477,997 (GRCm39)	missense	probably damaging	1.00
R8874:Map2	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
R8887:Map2	UTSW	1	66,454,758 (GRCm39)	missense	possibly damaging	0.48
R8948:Map2	UTSW	1	66,419,684 (GRCm39)	missense	probably damaging	1.00
R8950:Map2	UTSW	1	66,419,684 (GRCm39)	missense	probably damaging	1.00
R8998:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R8999:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R9072:Map2	UTSW	1	66,453,312 (GRCm39)	missense	probably damaging	1.00
R9088:Map2	UTSW	1	66,453,773 (GRCm39)	missense	probably damaging	1.00
R9089:Map2	UTSW	1	66,452,098 (GRCm39)	missense	probably benign	0.09
R9106:Map2	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
R9120:Map2	UTSW	1	66,453,218 (GRCm39)	missense	probably damaging	1.00
R9161:Map2	UTSW	1	66,477,503 (GRCm39)	missense	possibly damaging	0.65
R9464:Map2	UTSW	1	66,454,497 (GRCm39)	missense	probably damaging	1.00
R9589:Map2	UTSW	1	66,449,753 (GRCm39)	missense	probably benign
V8831:Map2	UTSW	1	66,455,004 (GRCm39)	missense	probably damaging	1.00
Z1177:Map2	UTSW	1	66,477,520 (GRCm39)	missense	probably damaging	0.96
Z1177:Map2	UTSW	1	66,419,839 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- AGGGTTTTCACCTATTCGGG -3'
(R):5'- GTGCCTGTTCTATACACATAGTCTC -3'

Sequencing Primer
(F):5'- GGGTTTTCACCTATTCGGGTTCTTC -3'
(R):5'- GGTTCATTGATATCAAAGTGAGATGC -3'

Posted On

2021-12-30