Mutagenetix > Incidental Mutation

Incidental Mutation 'R9113:Gsta5'

692367

Institutional Source

Beutler Lab

Gene Symbol

Gsta5

Ensembl Gene

ENSMUSG00000074179

Gene Name

glutathione S-transferase alpha 5

Synonyms

Gm10639

MMRRC Submission

068920-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R9113 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

78197210-78212807 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 78212667 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 213 (I213F)

Ref Sequence

ENSEMBL: ENSMUSP00000113635 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000119823] [ENSMUST00000121273]

AlphaFold

E9Q6L7

Predicted Effect

probably benign
Transcript: ENSMUST00000119823
AA Change: I213F

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000113211
Gene: ENSMUSG00000074179
AA Change: I213F

Domain	Start	End	E-Value	Type
Pfam:GST_N	5	77	3.7e-22	PFAM
Pfam:GST_C	99	192	5.9e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000121273
AA Change: I213F

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000113635
Gene: ENSMUSG00000074179
AA Change: I213F

Domain	Start	End	E-Value	Type
Pfam:GST_N	5	77	7.8e-20	PFAM
Pfam:GST_C	99	192	1.5e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.9%
20x: 99.6%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc9	T	A	6: 142,591,656 (GRCm39)	I791F	probably damaging	Het
Adamts15	A	G	9: 30,822,498 (GRCm39)	M389T	probably damaging	Het
Adgrb2	G	A	4: 129,910,877 (GRCm39)	V1065M	probably damaging	Het
Agbl1	C	A	7: 76,239,225 (GRCm39)	A827E	unknown	Het
Alox5ap	T	C	5: 149,216,015 (GRCm39)	F67S	probably damaging	Het
Ankrd11	T	C	8: 123,614,072 (GRCm39)	N2566S	possibly damaging	Het
Arhgap10	T	C	8: 77,985,701 (GRCm39)	D722G	probably damaging	Het
Atp5mf	A	G	5: 145,128,315 (GRCm39)	L4P	probably benign	Het
Bean1	T	C	8: 104,940,557 (GRCm39)	V130A	probably benign	Het
Ccdc88b	T	C	19: 6,833,213 (GRCm39)	E278G	probably damaging	Het
Cdhr2	A	G	13: 54,882,700 (GRCm39)	D1222G	probably benign	Het
Chrm4	T	A	2: 91,758,075 (GRCm39)	V161E	probably benign	Het
Ckap5	T	C	2: 91,426,144 (GRCm39)	S1335P	probably damaging	Het
Col11a1	T	A	3: 113,888,192 (GRCm39)	Y267*	probably null	Het
Col14a1	T	C	15: 55,201,825 (GRCm39)	Y38H	unknown	Het
Coro2a	C	T	4: 46,563,047 (GRCm39)	G36S		Het
Ctsc	A	T	7: 87,959,104 (GRCm39)	K461N	possibly damaging	Het
Dcaf1	A	G	9: 106,712,831 (GRCm39)		probably benign	Het
Dnah9	A	G	11: 65,880,713 (GRCm39)	I2628T	probably damaging	Het
Dnajb5	T	C	4: 42,953,233 (GRCm39)	I20T	probably damaging	Het
Dus2	C	T	8: 106,775,333 (GRCm39)	Q287*	probably null	Het
Fbxl17	A	G	17: 63,532,085 (GRCm39)	V586A	probably benign	Het
Glg1	A	T	8: 111,887,452 (GRCm39)		probably benign	Het
Gm4924	G	A	10: 82,214,113 (GRCm39)	C637Y	unknown	Het
Gorasp1	A	G	9: 119,757,442 (GRCm39)	F310S	probably damaging	Het
Igfn1	G	A	1: 135,883,328 (GRCm39)	T2726I	probably damaging	Het
Inppl1	G	T	7: 101,475,231 (GRCm39)	P940Q	probably benign	Het
Klk1b22	G	A	7: 43,765,692 (GRCm39)	V183M	possibly damaging	Het
Krt12	A	G	11: 99,309,378 (GRCm39)	M294T	probably damaging	Het
Krtap6-2	GAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCGTATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCAGAGCCACAGCCATAGCCAGAACCATAGCCACAGCCATAGCCAGAGCCATAGCCACAGCCATAGCCAGAGCCAGAGCCACAGCCATAGCCAGAGCCATAGCCACAGCCATAGCCA	GAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCGTATCCACAGCCATAGCCAGAGCCATATCCACAGCCATAGCCAGAGCCAGAGCCACAGCCATAGCCAGAACCATAGCCACAGCCATAGCCAGAGCCATAGCCACAGCCATAGCCAGAGCCAGAGCCACAGCCATAGCCAGAGCCATAGCCACAGCCATAGCCA	16: 89,216,613 (GRCm39)		probably benign	Het
Lep	T	C	6: 29,071,093 (GRCm39)	L139P	probably damaging	Het
Ltn1	T	C	16: 87,224,532 (GRCm39)	D64G	probably damaging	Het
Lyzl1	C	T	18: 4,168,604 (GRCm39)	A28V	probably null	Het
Mcam	T	C	9: 44,051,693 (GRCm39)	V483A	probably benign	Het
Mia2	C	T	12: 59,217,053 (GRCm39)		probably benign	Het
Mzf1	T	A	7: 12,778,279 (GRCm39)	H454L	probably damaging	Het
Pde10a	A	G	17: 9,197,782 (GRCm39)	T742A	probably benign	Het
Rapgef4	A	G	2: 71,861,493 (GRCm39)	Y61C	probably benign	Het
Ryr2	A	T	13: 11,618,741 (GRCm39)		probably benign	Het
Setbp1	G	T	18: 78,900,948 (GRCm39)	H906Q	probably damaging	Het
Slc12a4	A	G	8: 106,670,984 (GRCm39)	V1032A	probably benign	Het
Slc6a15	T	C	10: 103,236,140 (GRCm39)	M285T	probably damaging	Het
Spata31h1	G	A	10: 82,131,352 (GRCm39)	Q553*	probably null	Het
Steap2	A	G	5: 5,727,475 (GRCm39)	Y287H	probably damaging	Het
Tas2r135	T	G	6: 42,383,315 (GRCm39)	F285V	probably benign	Het
Tmco1	C	T	1: 167,136,132 (GRCm39)		probably benign	Het
Ttn	T	C	2: 76,618,538 (GRCm39)	T16249A	probably benign	Het
Usp8	A	G	2: 126,579,343 (GRCm39)	T299A	probably benign	Het
Vmn2r101	A	G	17: 19,811,288 (GRCm39)	I457M	possibly damaging	Het
Vps41	A	G	13: 19,023,883 (GRCm39)	E437G	probably benign	Het
Zfp638	A	T	6: 83,953,894 (GRCm39)	E1333V	probably damaging	Het

Other mutations in Gsta5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01764:Gsta5	APN	9	78,211,789 (GRCm39)	splice site	probably null
R0117:Gsta5	UTSW	9	78,211,700 (GRCm39)	missense	probably damaging	1.00
R0480:Gsta5	UTSW	9	78,210,099 (GRCm39)	missense	probably benign	0.08
R0645:Gsta5	UTSW	9	78,206,303 (GRCm39)	missense	possibly damaging	0.93
R2509:Gsta5	UTSW	9	78,202,089 (GRCm39)	start codon destroyed	probably null	1.00
R2510:Gsta5	UTSW	9	78,202,089 (GRCm39)	start codon destroyed	probably null	1.00
R2519:Gsta5	UTSW	9	78,211,721 (GRCm39)	missense	probably damaging	0.98
R4797:Gsta5	UTSW	9	78,211,679 (GRCm39)	missense	probably benign	0.01
R6174:Gsta5	UTSW	9	78,211,737 (GRCm39)	nonsense	probably null
R7570:Gsta5	UTSW	9	78,211,751 (GRCm39)	missense	possibly damaging	0.58

Predicted Primers

PCR Primer
(F):5'- ATGCTGACACTGGAGCTGTTG -3'
(R):5'- TGCGATGCTAGCAATCTGAGC -3'

Sequencing Primer
(F):5'- CACTGGAGCTGTTGCAAGG -3'
(R):5'- GATGCTAGCAATCTGAGCTCATAC -3'

Posted On

2021-12-30