Mutagenetix > Incidental Mutations

Incidental Mutation 'R9114:Dclk3'

692426

Institutional Source

Beutler Lab

Gene Symbol

Dclk3

Ensembl Gene

ENSMUSG00000032500

Gene Name

doublecortin-like kinase 3

Synonyms

Click-I, -II related, Dcamkl3

Accession Numbers

Genbank: NM_172928; MGI: 3039580

Is this an essential gene?

Possibly non essential (E-score: 0.304) question?

Stock #

R9114 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

111439081-111489118 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 111488615 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 773 (M773K)

Ref Sequence

ENSEMBL: ENSMUSP00000107510 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111879]

AlphaFold

Q8BWQ5

Predicted Effect

probably benign
Transcript: ENSMUST00000111879
AA Change: M773K

PolyPhen 2 Score 0.061 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000107510
Gene: ENSMUSG00000032500
AA Change: M773K

Domain	Start	End	E-Value	Type
low complexity region	2	29	N/A	INTRINSIC
Pfam:DCX	115	177	2.9e-17	PFAM
low complexity region	200	218	N/A	INTRINSIC
low complexity region	267	277	N/A	INTRINSIC
low complexity region	479	496	N/A	INTRINSIC
S_TKc	514	771	1.63e-110	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.5%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the protein kinase superfamily and the doublecortin family. Differently from the other two closely related family members (DCLK1 and DCLK2), the protein encoded by this gene contains only one N-terminal doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. The protein contains a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. [provided by RefSeq, Sep 2010]

Allele List at MGI

All alleles(3) : Targeted, other(2) Gene trapped(1)

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1a	T	A	5: 8,738,702	L1027*	probably null	Het
Actl11	T	A	9: 107,931,310	M944K	possibly damaging	Het
Afap1l2	T	C	19: 56,917,995	K491E	probably damaging	Het
Ahr	A	T	12: 35,511,165	D150E	probably damaging	Het
Arhgef1	T	A	7: 24,907,879	L17Q	probably damaging	Het
Bysl	A	G	17: 47,604,317	S169P		Het
C4b	A	T	17: 34,729,430	F1610I	probably damaging	Het
Chtf8	C	A	8: 106,885,849	A153S	probably benign	Het
Ciapin1	C	T	8: 94,831,772		probably null	Het
Cnnm1	A	G	19: 43,440,956	E171G	possibly damaging	Het
Crybb3	T	C	5: 113,077,541	N155S	probably benign	Het
Ddx18	A	T	1: 121,561,538	V260D	probably damaging	Het
Dmxl2	T	A	9: 54,400,037	N2216Y		Het
Epn2	T	C	11: 61,546,620	E42G	probably damaging	Het
Esrrg	C	A	1: 188,146,408	P229T	probably benign	Het
Esrrg	C	A	1: 188,146,409	P229Q	possibly damaging	Het
Ethe1	G	T	7: 24,606,218	R130L	probably benign	Het
Fgl2	G	A	5: 21,375,365	C235Y	probably damaging	Het
Fmnl1	C	T	11: 103,196,501	T890M	unknown	Het
Fsip2	C	G	2: 82,976,957	P1207A	probably benign	Het
Gc	C	T	5: 89,445,306	D85N	possibly damaging	Het
Gfi1	G	A	5: 107,721,504	R287W	probably damaging	Het
Gm3460	T	C	14: 6,619,567		probably null	Het
Hapln3	A	G	7: 79,121,964	F59S	probably benign	Het
Il24	G	A	1: 130,885,746	T38I	possibly damaging	Het
Izumo1	A	G	7: 45,627,159	D382G	probably benign	Het
Kdm5a	T	A	6: 120,405,926	Y739*	probably null	Het
Kif2b	T	A	11: 91,575,712	I582F	possibly damaging	Het
Klhdc1	A	G	12: 69,242,009	Y31C	probably damaging	Het
Lama1	G	A	17: 67,821,674	E3009K		Het
Mad2l1bp	G	A	17: 46,148,032	R191C	probably damaging	Het
Mark1	A	C	1: 184,912,064	S468A	probably damaging	Het
Mknk2	T	A	10: 80,668,989	N236Y	probably damaging	Het
Muc2	T	A	7: 141,701,414	C251*	probably null	Het
Nacad	T	C	11: 6,602,252	D313G	probably damaging	Het
Neb	G	T	2: 52,209,587	D4750E	probably benign	Het
Nlrp6	T	C	7: 140,926,419	S758P	probably damaging	Het
Nxt2	C	T	X: 142,237,751	A118V	possibly damaging	Het
Olfr61	A	C	7: 140,638,369	I223L	probably benign	Het
Olfr66	T	C	7: 103,881,362	T294A	possibly damaging	Het
Olfr918	A	G	9: 38,672,596	F283L	probably benign	Het
Oxtr	A	G	6: 112,489,520	V93A	probably damaging	Het
Pbx3	C	A	2: 34,213,259	D234Y	probably damaging	Het
Pde4b	A	T	4: 102,602,629	T554S	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452		probably benign	Het
Pid1	A	T	1: 84,038,234	Y151N	probably damaging	Het
Plcd4	A	G	1: 74,552,148	E234G	possibly damaging	Het
Pou6f1	A	G	15: 100,580,908	V373A	probably benign	Het
Ppp1r12c	T	C	7: 4,482,793	K685E	possibly damaging	Het
Rnf34	T	A	5: 122,861,894	V71D	probably damaging	Het
Rpap2	T	G	5: 107,598,290	H11Q	possibly damaging	Het
Skint2	A	G	4: 112,639,637	T247A	probably benign	Het
Stat5b	T	A	11: 100,801,524	N145Y	probably damaging	Het
Sv2b	A	G	7: 75,206,269	L91P	probably damaging	Het
Syt1	A	G	10: 108,504,515	I314T	probably damaging	Het
Tlk1	A	T	2: 70,742,158	N355K	probably benign	Het
Tnc	C	A	4: 63,972,736	M1636I	probably benign	Het
Trank1	C	A	9: 111,333,775	A34D	probably damaging	Het
Unc13c	A	G	9: 73,812,383	I1001T	probably benign	Het
Vdr	T	C	15: 97,867,255	D201G	probably benign	Het
Vmn2r115	A	T	17: 23,345,333	I160L	probably benign	Het
Wdr81	C	A	11: 75,444,424	R1772L	probably damaging	Het
Zfp457	T	C	13: 67,294,004	D169G	probably benign	Het

Other mutations in Dclk3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02027:Dclk3	APN	9	111467843	nonsense	probably null
IGL02125:Dclk3	APN	9	111469107	missense	probably damaging	1.00
IGL02547:Dclk3	APN	9	111469023	missense	probably damaging	1.00
IGL03393:Dclk3	APN	9	111488673	utr 3 prime	probably benign
G1citation:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
IGL02984:Dclk3	UTSW	9	111488575	missense	probably damaging	1.00
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0109:Dclk3	UTSW	9	111467670	missense	possibly damaging	0.93
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0238:Dclk3	UTSW	9	111482628	missense	probably damaging	0.99
R0432:Dclk3	UTSW	9	111484935	missense	probably damaging	1.00
R0440:Dclk3	UTSW	9	111469163	missense	probably damaging	1.00
R0530:Dclk3	UTSW	9	111482721	missense	probably damaging	1.00
R1024:Dclk3	UTSW	9	111469070	missense	possibly damaging	0.95
R1443:Dclk3	UTSW	9	111469020	missense	probably benign	0.01
R1474:Dclk3	UTSW	9	111469236	missense	probably benign	0.43
R1479:Dclk3	UTSW	9	111468546	missense	probably benign
R1482:Dclk3	UTSW	9	111467820	missense	possibly damaging	0.90
R1543:Dclk3	UTSW	9	111468054	missense	probably benign	0.04
R1552:Dclk3	UTSW	9	111488579	missense	probably damaging	1.00
R1559:Dclk3	UTSW	9	111469208	missense	probably damaging	1.00
R2011:Dclk3	UTSW	9	111468354	missense	probably benign	0.00
R2369:Dclk3	UTSW	9	111488542	missense	probably benign	0.16
R4111:Dclk3	UTSW	9	111469080	missense	probably damaging	0.99
R4510:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4511:Dclk3	UTSW	9	111467992	missense	probably benign	0.01
R4592:Dclk3	UTSW	9	111467895	missense	probably damaging	1.00
R4604:Dclk3	UTSW	9	111469185	missense	probably damaging	1.00
R4857:Dclk3	UTSW	9	111468648	missense	probably benign
R4932:Dclk3	UTSW	9	111468042	missense	possibly damaging	0.56
R5045:Dclk3	UTSW	9	111467788	missense	probably damaging	0.99
R5233:Dclk3	UTSW	9	111468681	missense	probably benign
R5338:Dclk3	UTSW	9	111469059	missense	possibly damaging	0.95
R5463:Dclk3	UTSW	9	111469260	missense	probably benign	0.26
R6822:Dclk3	UTSW	9	111439337	missense	probably benign	0.03
R6995:Dclk3	UTSW	9	111467700	missense	possibly damaging	0.88
R7187:Dclk3	UTSW	9	111484996	missense	probably damaging	1.00
R7532:Dclk3	UTSW	9	111467528	missense	probably benign	0.03
R7534:Dclk3	UTSW	9	111468218	missense	probably benign
R7734:Dclk3	UTSW	9	111469095	missense	probably damaging	1.00
R8326:Dclk3	UTSW	9	111467534	missense	probably damaging	0.96
R8372:Dclk3	UTSW	9	111485013	missense	probably damaging	0.98
R8388:Dclk3	UTSW	9	111482745	missense	probably damaging	1.00
R8423:Dclk3	UTSW	9	111468719	missense	possibly damaging	0.93
R8493:Dclk3	UTSW	9	111468147	missense	probably benign	0.06
R9013:Dclk3	UTSW	9	111468498	missense	probably benign	0.31
R9338:Dclk3	UTSW	9	111439305	missense	unknown
R9412:Dclk3	UTSW	9	111482751	critical splice donor site	probably null
X0020:Dclk3	UTSW	9	111485075	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCCACAGTGCTAATGTGGATG -3'
(R):5'- TGTGGAGAAGGGTCTTGCAC -3'

Sequencing Primer
(F):5'- ATGTGTGGGCTCCTACCTCATG -3'
(R):5'- ACAGCCTTGCTTGTAGACTAGACG -3'

Posted On

2021-12-30