Mutagenetix > Incidental Mutation

Incidental Mutation 'R9116:Or2j3'

692570

Institutional Source

Beutler Lab

Gene Symbol

Or2j3

Ensembl Gene

ENSMUSG00000054940

Gene Name

olfactory receptor family 2 subfamily J member 3

Synonyms

Olfr137, MOR256-18, GA_x6K02T2PSCP-2749525-2748587

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.432) question?

Stock #

R9116 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38615412-38616350 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 38615654 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 233 (T233A)

Ref Sequence

ENSEMBL: ENSMUSP00000150173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057394] [ENSMUST00000213505]

AlphaFold

Q8VFC2

Predicted Effect

probably benign
Transcript: ENSMUST00000057394
AA Change: T233A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000051537
Gene: ENSMUSG00000054940
AA Change: T233A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	308	4e-49	PFAM
Pfam:7tm_1	42	291	4.5e-28	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213505
AA Change: T233A

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (76/76)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca7	T	A	10: 79,838,973 (GRCm39)	L689Q		Het
Abcc2	G	A	19: 43,793,391 (GRCm39)	V348M	probably benign	Het
Abhd5	T	C	9: 122,196,992 (GRCm39)	S60P	probably benign	Het
Adamts10	C	A	17: 33,756,330 (GRCm39)	H324Q	probably benign	Het
Akap1	A	G	11: 88,723,165 (GRCm39)	L823P	probably damaging	Het
Aoc1	G	A	6: 48,885,522 (GRCm39)	V676I	probably damaging	Het
Arhgef12	T	C	9: 42,893,241 (GRCm39)		probably benign	Het
Arl4d	A	C	11: 101,557,620 (GRCm39)	S49R	possibly damaging	Het
Atr	A	G	9: 95,747,851 (GRCm39)	I378V	probably benign	Het
Bicd1	G	A	6: 149,385,674 (GRCm39)	V136I	probably benign	Het
Brd1	A	T	15: 88,585,374 (GRCm39)	L820H	possibly damaging	Het
Cfap70	T	C	14: 20,497,590 (GRCm39)	T55A	probably benign	Het
Cog7	G	T	7: 121,570,561 (GRCm39)	N182K	probably damaging	Het
Col4a4	T	A	1: 82,431,752 (GRCm39)	T1511S	unknown	Het
Cr2	A	T	1: 194,840,977 (GRCm39)	Y438*	probably null	Het
Ctnnbl1	T	A	2: 157,648,623 (GRCm39)	V198E	probably damaging	Het
Fam117b	C	T	1: 60,018,456 (GRCm39)	Q58*	probably null	Het
Fam171a2	A	G	11: 102,330,519 (GRCm39)	Y288H	probably damaging	Het
Fam186a	A	T	15: 99,840,472 (GRCm39)	I1924K	possibly damaging	Het
Fancd2	A	G	6: 113,532,180 (GRCm39)	I498V	probably benign	Het
Fat3	T	A	9: 15,909,421 (GRCm39)	I2194F	probably benign	Het
Flg2	T	C	3: 93,109,591 (GRCm39)	S540P	unknown	Het
Gbp9	T	C	5: 105,231,695 (GRCm39)	Y297C		Het
Gck	T	G	11: 5,854,377 (GRCm39)	N283H	possibly damaging	Het
Gm13941	A	T	2: 110,935,146 (GRCm39)	L28Q	unknown	Het
Gpr155	A	G	2: 73,204,109 (GRCm39)	I235T	possibly damaging	Het
Junb	A	C	8: 85,704,052 (GRCm39)	L336R	probably damaging	Het
Krt14	A	T	11: 100,095,904 (GRCm39)	M218K	probably benign	Het
Leprotl1	A	G	8: 34,604,967 (GRCm39)	V102A	probably benign	Het
Lrrc8d	T	C	5: 105,961,908 (GRCm39)	F773L	probably damaging	Het
Mad2l1bp	G	A	17: 46,458,958 (GRCm39)	R191C	probably damaging	Het
Man1c1	C	T	4: 134,311,705 (GRCm39)	V274M	possibly damaging	Het
Mtor	C	A	4: 148,637,198 (GRCm39)	P2466T	probably benign	Het
Mycbpap	C	A	11: 94,398,032 (GRCm39)		probably benign	Het
Myef2l	G	A	3: 10,153,593 (GRCm39)	V121M	probably damaging	Het
Myo18b	T	C	5: 112,975,862 (GRCm39)	E1329G	probably damaging	Het
Nipbl	A	T	15: 8,380,340 (GRCm39)	D817E	probably benign	Het
Nthl1	C	T	17: 24,853,753 (GRCm39)	Q133*	probably null	Het
Nup133	A	G	8: 124,660,155 (GRCm39)	M381T	probably benign	Het
Or13c7	T	C	4: 43,854,602 (GRCm39)	C98R	probably damaging	Het
Or51t4	T	C	7: 102,598,527 (GRCm39)	I285T	possibly damaging	Het
Or7e175	T	C	9: 20,048,633 (GRCm39)	Y74H	probably damaging	Het
Or7g20	T	C	9: 18,946,773 (GRCm39)	M118T	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Pmm1	C	T	15: 81,839,896 (GRCm39)	R143H	probably damaging	Het
Pmpcb	A	T	5: 21,961,608 (GRCm39)	I422F	probably damaging	Het
Prpf8	T	A	11: 75,380,589 (GRCm39)	H97Q	possibly damaging	Het
Psat1	A	G	19: 15,898,332 (GRCm39)	L30P	probably damaging	Het
Rag1	T	C	2: 101,472,820 (GRCm39)	D774G	probably damaging	Het
Rag1	C	T	2: 101,475,137 (GRCm39)	A2T	probably benign	Het
Rccd1	G	T	7: 79,970,728 (GRCm39)	F17L	probably damaging	Het
Rhobtb1	T	A	10: 69,106,579 (GRCm39)	D443E	probably damaging	Het
Rnf13	A	G	3: 57,709,866 (GRCm39)		probably null	Het
Ryr2	A	G	13: 11,587,185 (GRCm39)	S4699P	possibly damaging	Het
Scart1	A	G	7: 139,808,277 (GRCm39)	T754A	probably benign	Het
Sdc3	T	C	4: 130,546,352 (GRCm39)	V237A	probably benign	Het
Serinc5	T	A	13: 92,797,514 (GRCm39)		probably benign	Het
Sertad4	A	G	1: 192,528,973 (GRCm39)	I281T	probably benign	Het
Shroom1	T	C	11: 53,354,490 (GRCm39)	S137P	probably damaging	Het
Slurp1	C	T	15: 74,599,450 (GRCm39)	G20D	probably damaging	Het
Sncb	T	A	13: 54,910,512 (GRCm39)	N75Y	probably damaging	Het
Spag8	C	T	4: 43,653,231 (GRCm39)	G77S	unknown	Het
Spink14	A	T	18: 44,164,059 (GRCm39)	I76F	probably damaging	Het
Srfbp1	A	T	18: 52,623,102 (GRCm39)	E372D	possibly damaging	Het
Synpo	A	T	18: 60,735,599 (GRCm39)	N782K	probably damaging	Het
Syt7	T	C	19: 10,421,373 (GRCm39)	M519T	probably damaging	Het
Tlr5	C	T	1: 182,802,160 (GRCm39)	P488L	probably benign	Het
Tmod3	A	T	9: 75,412,202 (GRCm39)	I315N	probably damaging	Het
Ttc12	T	C	9: 49,364,757 (GRCm39)	M340V	probably benign	Het
Ttn	T	A	2: 76,710,959 (GRCm39)	I8392F	unknown	Het
Ttn	A	G	2: 76,769,232 (GRCm39)	V2822A	unknown	Het
Ube3b	C	T	5: 114,542,837 (GRCm39)		probably benign	Het
Ubr4	C	A	4: 139,145,785 (GRCm39)	N238K		Het
Vmn2r12	T	C	5: 109,233,885 (GRCm39)	T776A	probably damaging	Het
Vps50	G	A	6: 3,588,091 (GRCm39)		probably benign	Het
Vwa3a	A	G	7: 120,366,470 (GRCm39)	D165G		Het
Wdr17	T	A	8: 55,114,605 (GRCm39)	H644L	probably damaging	Het
Zfp628	T	C	7: 4,924,202 (GRCm39)	V808A	probably benign	Het
Zscan21	T	A	5: 138,123,937 (GRCm39)	D205E	probably damaging	Het

Other mutations in Or2j3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02131:Or2j3	APN	17	38,615,539 (GRCm39)	missense	probably benign	0.03
IGL03169:Or2j3	APN	17	38,615,992 (GRCm39)	missense	probably damaging	1.00
IGL03392:Or2j3	APN	17	38,615,786 (GRCm39)	missense	probably benign	0.02
R0055:Or2j3	UTSW	17	38,615,702 (GRCm39)	missense	possibly damaging	0.84
R0055:Or2j3	UTSW	17	38,615,702 (GRCm39)	missense	possibly damaging	0.84
R0496:Or2j3	UTSW	17	38,615,549 (GRCm39)	missense	probably damaging	0.99
R0761:Or2j3	UTSW	17	38,616,282 (GRCm39)	missense	probably benign	0.00
R1126:Or2j3	UTSW	17	38,615,579 (GRCm39)	missense	probably damaging	0.97
R1835:Or2j3	UTSW	17	38,616,203 (GRCm39)	missense	probably benign	0.02
R3727:Or2j3	UTSW	17	38,616,310 (GRCm39)	missense	possibly damaging	0.64
R5556:Or2j3	UTSW	17	38,615,964 (GRCm39)	missense	possibly damaging	0.71
R5979:Or2j3	UTSW	17	38,616,083 (GRCm39)	missense	probably benign
R6430:Or2j3	UTSW	17	38,616,249 (GRCm39)	missense	probably benign	0.04
R6433:Or2j3	UTSW	17	38,616,304 (GRCm39)	missense	probably damaging	1.00
R7400:Or2j3	UTSW	17	38,616,222 (GRCm39)	missense	possibly damaging	0.91
R8053:Or2j3	UTSW	17	38,616,101 (GRCm39)	missense	probably benign
R8199:Or2j3	UTSW	17	38,615,444 (GRCm39)	missense	probably benign	0.31
R8446:Or2j3	UTSW	17	38,615,638 (GRCm39)	missense	probably benign	0.01
R8865:Or2j3	UTSW	17	38,615,872 (GRCm39)	missense	probably damaging	0.97
R9756:Or2j3	UTSW	17	38,615,971 (GRCm39)	missense	probably benign	0.13

Predicted Primers

PCR Primer
(F):5'- CACAGTGGGAGGCTTATCTC -3'
(R):5'- GTGGCCATCGCAAAGTAGAC -3'

Sequencing Primer
(F):5'- CACAGTGGGAGGCTTATCTCATTTC -3'
(R):5'- AGTAGACCACTTCTTCTGTGAAG -3'

Posted On

2021-12-30