Mutagenetix > Incidental Mutation

Incidental Mutation 'R9121:Cnnm3'

692755

Institutional Source

Beutler Lab

Gene Symbol

Cnnm3

Ensembl Gene

ENSMUSG00000001138

Gene Name

cyclin M3

Synonyms

Acdp3

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R9121 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

36550948-36567318 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 36563158 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 707 (C707*)

Ref Sequence

ENSEMBL: ENSMUSP00000095383 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001166] [ENSMUST00000097776]

AlphaFold

Q32NY4

Predicted Effect

probably benign
Transcript: ENSMUST00000001166

SMART Domains

Protein: ENSMUSP00000001166
Gene: ENSMUSG00000001138

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	25	35	N/A	INTRINSIC
low complexity region	87	104	N/A	INTRINSIC
low complexity region	128	140	N/A	INTRINSIC
Pfam:DUF21	142	295	3.2e-10	PFAM
Blast:CBS	329	379	9e-12	BLAST
Pfam:CBS	388	452	6.4e-6	PFAM
Blast:cNMP	527	668	2e-38	BLAST

Predicted Effect

probably null
Transcript: ENSMUST00000097776
AA Change: C707*

SMART Domains

Protein: ENSMUSP00000095383
Gene: ENSMUSG00000001138
AA Change: C707*

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
low complexity region	25	35	N/A	INTRINSIC
low complexity region	87	104	N/A	INTRINSIC
low complexity region	128	140	N/A	INTRINSIC
Pfam:DUF21	142	300	1e-19	PFAM
Blast:CBS	329	379	9e-12	BLAST
Pfam:CBS	388	452	5.1e-6	PFAM
Blast:cNMP	527	668	1e-37	BLAST

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (44/44)

Allele List at MGI

All alleles(3) : Gene trapped(3)

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy2	A	T	13: 68,820,078 (GRCm39)	N720K	probably benign	Het
Ampd1	C	T	3: 103,005,998 (GRCm39)	Q603*	probably null	Het
Arap2	G	A	5: 62,906,326 (GRCm39)	T231M	possibly damaging	Het
Atp2b4	TCT	TCTGCT	1: 133,629,463 (GRCm39)		probably benign	Het
Cap1	C	T	4: 122,761,516 (GRCm39)	R122Q	probably damaging	Het
Cdh10	G	A	15: 19,011,074 (GRCm39)	V586M	probably damaging	Het
Clvs2	A	T	10: 33,389,331 (GRCm39)	V303E	possibly damaging	Het
Crtc3	A	G	7: 80,242,323 (GRCm39)	F497L	probably damaging	Het
Csmd3	T	C	15: 47,683,774 (GRCm39)	Y949C		Het
D5Ertd579e	A	T	5: 36,772,778 (GRCm39)	I539N	probably damaging	Het
Dctpp1	T	C	7: 126,856,456 (GRCm39)	R89G	probably damaging	Het
Dnah7c	T	A	1: 46,704,650 (GRCm39)	V2221E	probably damaging	Het
Dnah7c	C	A	1: 46,816,896 (GRCm39)	L3628I	probably benign	Het
E030025P04Rik	A	G	11: 109,034,738 (GRCm39)	F50S	unknown	Het
Edrf1	CGG	CG	7: 133,258,770 (GRCm39)		probably null	Het
Epb41l1	T	G	2: 156,364,487 (GRCm39)	S999A	probably benign	Het
Fbxw10	T	A	11: 62,738,153 (GRCm39)	C16S	possibly damaging	Het
Fbxw19	G	A	9: 109,324,890 (GRCm39)	P9L	probably damaging	Het
Gpi1	A	T	7: 33,907,114 (GRCm39)	Y327N	probably damaging	Het
Hook3	A	G	8: 26,525,195 (GRCm39)	L637P	probably damaging	Het
Idh3a	T	C	9: 54,493,829 (GRCm39)		probably null	Het
Ighv1-56	T	A	12: 115,206,634 (GRCm39)	T47S	probably benign	Het
Iglc2	A	C	16: 19,017,294 (GRCm39)	C103G	probably damaging	Het
Mbd5	A	G	2: 49,148,102 (GRCm39)	T771A	possibly damaging	Het
Mcm2	C	A	6: 88,861,019 (GRCm39)	R893L	probably benign	Het
Mettl15	A	T	2: 109,104,948 (GRCm39)	V19E		Het
Mier2	G	A	10: 79,377,594 (GRCm39)	A453V		Het
Mlf1	T	C	3: 67,307,054 (GRCm39)	S255P	probably benign	Het
Obsl1	T	C	1: 75,482,636 (GRCm39)	D78G	possibly damaging	Het
Or5w14	T	A	2: 87,541,975 (GRCm39)	I92F	probably damaging	Het
Or8b55	T	A	9: 38,726,976 (GRCm39)	M59K	probably damaging	Het
Otof	T	C	5: 30,536,462 (GRCm39)	K1340E	probably benign	Het
Pla2g4c	A	G	7: 13,091,010 (GRCm39)	D589G	probably damaging	Het
Qrfpr	A	G	3: 36,235,156 (GRCm39)	V278A	probably damaging	Het
Qrich2	T	A	11: 116,347,272 (GRCm39)	H1184L	unknown	Het
Rnaset2a	A	G	17: 8,347,549 (GRCm39)	F250S	possibly damaging	Het
Sfrp5	T	C	19: 42,190,356 (GRCm39)	Y32C	probably damaging	Het
Slc44a3	A	G	3: 121,254,786 (GRCm39)	S611P	probably benign	Het
Slco1a1	C	A	6: 141,892,542 (GRCm39)		probably benign	Het
Synj2	A	T	17: 6,040,599 (GRCm39)	D225V	probably damaging	Het
Tacc1	G	T	8: 25,659,255 (GRCm39)	S570R	probably damaging	Het
Trim7	T	A	11: 48,740,674 (GRCm39)	L257Q	probably damaging	Het
Trpm8	T	A	1: 88,312,423 (GRCm39)	N1101K	probably benign	Het
Unc79	C	T	12: 102,968,095 (GRCm39)	T107M	probably damaging	Het
Usp54	A	G	14: 20,631,523 (GRCm39)		probably null	Het
Zzef1	T	A	11: 72,756,946 (GRCm39)	Y1150*	probably null	Het

Other mutations in Cnnm3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01310:Cnnm3	APN	1	36,551,956 (GRCm39)	missense	probably benign	0.12
IGL02679:Cnnm3	APN	1	36,559,239 (GRCm39)	missense	probably benign	0.01
IGL02700:Cnnm3	APN	1	36,552,189 (GRCm39)	missense	probably damaging	1.00
IGL03165:Cnnm3	APN	1	36,564,313 (GRCm39)	unclassified	probably benign
R0003:Cnnm3	UTSW	1	36,563,124 (GRCm39)	missense	probably benign	0.02
R0358:Cnnm3	UTSW	1	36,560,303 (GRCm39)	missense	probably damaging	0.98
R1129:Cnnm3	UTSW	1	36,552,097 (GRCm39)	missense	probably damaging	1.00
R1772:Cnnm3	UTSW	1	36,558,038 (GRCm39)	missense	probably damaging	1.00
R2929:Cnnm3	UTSW	1	36,563,140 (GRCm39)	missense	possibly damaging	0.55
R3153:Cnnm3	UTSW	1	36,560,303 (GRCm39)	missense	probably damaging	0.98
R3154:Cnnm3	UTSW	1	36,560,303 (GRCm39)	missense	probably damaging	0.98
R5376:Cnnm3	UTSW	1	36,559,759 (GRCm39)	missense	probably damaging	1.00
R5810:Cnnm3	UTSW	1	36,564,280 (GRCm39)	missense	probably benign	0.02
R6389:Cnnm3	UTSW	1	36,559,603 (GRCm39)	missense	probably damaging	0.99
R8288:Cnnm3	UTSW	1	36,551,074 (GRCm39)	missense	possibly damaging	0.93
R8951:Cnnm3	UTSW	1	36,558,019 (GRCm39)	splice site	probably benign
R8959:Cnnm3	UTSW	1	36,558,096 (GRCm39)	missense	probably damaging	1.00
R9068:Cnnm3	UTSW	1	36,551,962 (GRCm39)	missense	probably damaging	1.00
X0064:Cnnm3	UTSW	1	36,552,061 (GRCm39)	missense	probably damaging	1.00
Z1177:Cnnm3	UTSW	1	36,552,114 (GRCm39)	missense	possibly damaging	0.96

Predicted Primers

PCR Primer
(F):5'- CTGTGATCCCAGCAACACTTG -3'
(R):5'- CTAACCAGGTGTCTCATGTCCC -3'

Sequencing Primer
(F):5'- GTGATCCCAGCAACACTTGTATGG -3'
(R):5'- ATGTCCCCCTGTCTTCTAGAAAAAG -3'

Posted On

2021-12-30