Mutagenetix > Incidental Mutation

Incidental Mutation 'R9130:Acaca'

693567

Institutional Source

Beutler Lab

Gene Symbol

Acaca

Ensembl Gene

ENSMUSG00000020532

Gene Name

acetyl-Coenzyme A carboxylase alpha

Synonyms

LOC327983, Acac, A530025K05Rik, acetyl-CoA carboxylase, Acc1

MMRRC Submission

068928-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9130 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

84129672-84401651 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 84311319 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Serine at position 1380 (L1380S)

Ref Sequence

ENSEMBL: ENSMUSP00000020843 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020843] [ENSMUST00000103201] [ENSMUST00000183887]

AlphaFold

Q5SWU9

Predicted Effect

probably damaging
Transcript: ENSMUST00000020843
AA Change: L1380S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000020843
Gene: ENSMUSG00000020532
AA Change: L1380S

Domain	Start	End	E-Value	Type
Pfam:CPSase_L_chain	116	236	4.7e-33	PFAM
Pfam:CPSase_L_D2	272	472	2.5e-55	PFAM
Pfam:ATP-grasp	280	443	4.3e-7	PFAM
Pfam:ATP-grasp_4	282	442	1.9e-11	PFAM
Pfam:Dala_Dala_lig_C	284	440	5.4e-7	PFAM
Biotin_carb_C	506	613	3.76e-24	SMART
low complexity region	708	725	N/A	INTRINSIC
Pfam:Biotin_lipoyl	751	817	9.8e-19	PFAM
Pfam:ACC_central	818	1568	2.1e-288	PFAM
Pfam:Carboxyl_trans	1668	2222	1.6e-185	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000103201
AA Change: L1380S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000099490
Gene: ENSMUSG00000020532
AA Change: L1380S

Domain	Start	End	E-Value	Type
Pfam:CPSase_L_chain	116	236	6.7e-29	PFAM
Pfam:ATP-grasp_4	239	442	2e-15	PFAM
Pfam:CPSase_L_D2	272	472	3.3e-55	PFAM
Pfam:Dala_Dala_lig_C	279	440	3e-7	PFAM
Pfam:ATP-grasp	281	442	1.1e-6	PFAM
Biotin_carb_C	506	613	3.76e-24	SMART
low complexity region	708	725	N/A	INTRINSIC
Pfam:Biotin_lipoyl	751	817	3.7e-18	PFAM
Pfam:ACC_central	818	1568	3.5e-253	PFAM
Pfam:Carboxyl_trans	1668	2222	2.7e-175	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000183887
AA Change: L201S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000139378
Gene: ENSMUSG00000020532
AA Change: L201S

Domain	Start	End	E-Value	Type
Pfam:ACC_central	1	228	8.6e-57	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

99% (87/88)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mice display embryonic lethality before embryo turning with growth arrest at the egg cylinder stage. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abr	A	G	11: 76,451,927 (GRCm38)	C585R	possibly damaging	Het
Aifm2	A	C	10: 61,727,726 (GRCm38)	Q125P	probably null	Het
Ak5	A	T	3: 152,472,932 (GRCm38)	L482*	probably null	Het
Aldh3a2	T	C	11: 61,248,932 (GRCm38)	Q458R	probably benign	Het
Ank2	G	A	3: 127,016,916 (GRCm38)	T524I		Het
Apoc3	A	T	9: 46,235,183 (GRCm38)	S25T	unknown	Het
Ash1l	A	T	3: 89,058,541 (GRCm38)	R2417*	probably null	Het
Bbs12	A	G	3: 37,319,056 (GRCm38)		probably benign	Het
Birc6	T	A	17: 74,612,151 (GRCm38)	I1992N		Het
Brd9	G	A	13: 73,944,787 (GRCm38)	V299M	probably damaging	Het
Brwd1	A	T	16: 96,064,930 (GRCm38)	N217K	probably damaging	Het
C3	T	C	17: 57,211,678 (GRCm38)	Y1235C	probably damaging	Het
Cacna1c	T	C	6: 118,613,946 (GRCm38)	Y1538C		Het
Ccdc172	T	A	19: 58,537,347 (GRCm38)	H212Q	possibly damaging	Het
Cdc6	A	G	11: 98,912,173 (GRCm38)	D313G	probably damaging	Het
Cyp2e1	T	C	7: 140,773,109 (GRCm38)	V353A	probably damaging	Het
Dlg2	G	T	7: 92,431,050 (GRCm38)	V711F	probably damaging	Het
Dmac2	T	C	7: 25,621,023 (GRCm38)	F49S	probably damaging	Het
Dnah7b	A	G	1: 46,134,514 (GRCm38)	K660E	probably benign	Het
Dusp8	A	T	7: 142,088,418 (GRCm38)	S106T	probably benign	Het
Dynll1	T	A	5: 115,300,545 (GRCm38)	I34F	probably benign	Het
Epb41l4b	A	G	4: 57,103,447 (GRCm38)	F130L	possibly damaging	Het
Erc2	T	C	14: 28,029,461 (GRCm38)	Y705H	probably benign	Het
Exosc8	T	A	3: 54,731,082 (GRCm38)	L160F	probably damaging	Het
F5	A	G	1: 164,174,261 (GRCm38)	T178A	probably benign	Het
F7	C	A	8: 13,035,059 (GRCm38)	P362T	probably damaging	Het
Fkbp3	T	C	12: 65,065,793 (GRCm38)	K156E	possibly damaging	Het
Folh1	G	A	7: 86,719,705 (GRCm38)	T738I	probably benign	Het
Gatad2b	C	T	3: 90,348,629 (GRCm38)	A134V	probably benign	Het
Glud1	T	A	14: 34,335,392 (GRCm38)	W338R		Het
Gm21149	T	C	5: 15,475,263 (GRCm38)	K62E	possibly damaging	Het
Gm32742	A	G	9: 51,138,749 (GRCm38)	Y1517H	probably damaging	Het
Grik5	G	A	7: 25,068,004 (GRCm38)		probably benign	Het
Gse1	A	T	8: 120,568,313 (GRCm38)	E391V	unknown	Het
Gxylt2	C	T	6: 100,733,368 (GRCm38)	Q157*	probably null	Het
Hbq1b	C	T	11: 32,287,092 (GRCm38)	T27I	probably damaging	Het
Hoxd13	T	A	2: 74,669,038 (GRCm38)	D243E	probably benign	Het
Hsd3b3	T	A	3: 98,743,895 (GRCm38)	I80F	possibly damaging	Het
Hsf2bp	A	T	17: 32,011,108 (GRCm38)		probably benign	Het
Itch	G	A	2: 155,210,125 (GRCm38)		probably benign	Het
Kat14	T	C	2: 144,373,822 (GRCm38)	F76L	probably benign	Het
Kdm6b	T	C	11: 69,404,598 (GRCm38)	T948A	unknown	Het
Kmt2c	A	T	5: 25,311,104 (GRCm38)	H2580Q	probably benign	Het
Lrp1	T	C	10: 127,546,412 (GRCm38)	T3727A	probably benign	Het
Lrrc8a	A	G	2: 30,257,030 (GRCm38)	I619V	possibly damaging	Het
Mansc1	C	A	6: 134,609,988 (GRCm38)	G409W	probably damaging	Het
Map3k11	C	A	19: 5,696,010 (GRCm38)	L418I	possibly damaging	Het
Mettl4	T	A	17: 94,735,485 (GRCm38)	I308L	possibly damaging	Het
Myo9a	A	G	9: 59,832,231 (GRCm38)	D742G	probably damaging	Het
Mypn	T	C	10: 63,192,873 (GRCm38)	Q137R	probably benign	Het
Neurod4	A	T	10: 130,270,558 (GRCm38)	Y282*	probably null	Het
Nup210	A	G	6: 91,043,817 (GRCm38)	F965S	probably benign	Het
Oplah	T	C	15: 76,300,898 (GRCm38)	T872A	possibly damaging	Het
Or1ab2	G	T	8: 72,109,853 (GRCm38)	G96C	probably damaging	Het
Or4b12	A	G	2: 90,266,014 (GRCm38)	C139R	probably damaging	Het
Or5m8	T	G	2: 85,992,475 (GRCm38)	Y219*	probably null	Het
Or6c68	A	G	10: 129,322,028 (GRCm38)	N135S	probably benign	Het
Pkn2	A	T	3: 142,809,484 (GRCm38)	D696E	possibly damaging	Het
Pla2r1	A	T	2: 60,495,385 (GRCm38)		probably benign	Het
Plpp1	A	T	13: 112,851,504 (GRCm38)	I54L		Het
Ppp4r2	T	G	6: 100,865,152 (GRCm38)	N191K	probably damaging	Het
Ptpdc1	G	T	13: 48,586,179 (GRCm38)	P592Q	probably benign	Het
Qrich2	A	T	11: 116,456,866 (GRCm38)	L1044*	probably null	Het
Rb1cc1	A	G	1: 6,244,885 (GRCm38)	I421V	probably damaging	Het
Ripor3	A	T	2: 167,981,347 (GRCm38)	C881*	probably null	Het
Rreb1	T	C	13: 37,930,306 (GRCm38)	F547S	probably benign	Het
Scel	T	C	14: 103,533,310 (GRCm38)	V60A	probably benign	Het
Shank3	G	A	15: 89,558,216 (GRCm38)	A1771T	probably benign	Het
Slc6a20a	A	G	9: 123,640,566 (GRCm38)		probably null	Het
Slfn5	C	T	11: 82,960,620 (GRCm38)	T581I	probably damaging	Het
Ssh3	A	C	19: 4,264,085 (GRCm38)	V412G	probably damaging	Het
Stx17	T	G	4: 48,159,071 (GRCm38)		probably benign	Het
Sun3	T	G	11: 9,018,170 (GRCm38)	T274P	probably benign	Het
Tm9sf1	T	C	14: 55,638,007 (GRCm38)	T427A	probably damaging	Het
Tmem114	T	A	16: 8,412,119 (GRCm38)	I140F		Het
Tmem40	T	C	6: 115,734,019 (GRCm38)	R167G	possibly damaging	Het
Top3a	C	A	11: 60,750,575 (GRCm38)		probably null	Het
Trafd1	A	G	5: 121,378,510 (GRCm38)	V210A	probably benign	Het
Trim66	T	C	7: 109,477,689 (GRCm38)	I348V	possibly damaging	Het
Ttn	G	A	2: 76,848,828 (GRCm38)	A10850V	unknown	Het
Unc80	G	A	1: 66,638,085 (GRCm38)	A2058T	possibly damaging	Het
Upp2	T	C	2: 58,778,008 (GRCm38)	Y238H	probably damaging	Het
Vmn1r224	T	A	17: 20,419,980 (GRCm38)	I273N	probably damaging	Het
Vps13c	T	C	9: 67,929,523 (GRCm38)	S1768P	probably damaging	Het
Zfp26	G	A	9: 20,437,427 (GRCm38)	H614Y	probably damaging	Het
Zfp263	C	T	16: 3,749,837 (GRCm38)	T672I	probably benign	Het
Zfp641	T	A	15: 98,288,851 (GRCm38)	Q297L	probably benign	Het
Zfp944	T	C	17: 22,341,050 (GRCm38)	E25G	probably damaging	Het
Zfp994	T	C	17: 22,200,000 (GRCm38)	E656G	unknown	Het
Zfyve19	A	G	2: 119,214,849 (GRCm38)	D206G	probably damaging	Het

Other mutations in Acaca

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00544:Acaca	APN	11	84,278,917 (GRCm38)	missense	probably damaging	1.00
IGL01134:Acaca	APN	11	84,251,279 (GRCm38)	missense	probably benign	0.22
IGL01446:Acaca	APN	11	84,260,631 (GRCm38)	missense	probably damaging	1.00
IGL01591:Acaca	APN	11	84,243,320 (GRCm38)	missense	probably damaging	1.00
IGL01663:Acaca	APN	11	84,277,802 (GRCm38)	missense	possibly damaging	0.85
IGL01767:Acaca	APN	11	84,320,542 (GRCm38)	missense	probably benign	0.01
IGL02206:Acaca	APN	11	84,260,747 (GRCm38)	nonsense	probably null
IGL02335:Acaca	APN	11	84,214,258 (GRCm38)	missense	possibly damaging	0.84
IGL02477:Acaca	APN	11	84,307,168 (GRCm38)	splice site	probably benign
IGL02515:Acaca	APN	11	84,262,403 (GRCm38)	missense	probably benign
IGL02651:Acaca	APN	11	84,245,204 (GRCm38)	splice site	probably benign
IGL02805:Acaca	APN	11	84,223,133 (GRCm38)	splice site	probably benign
IGL03328:Acaca	APN	11	84,320,529 (GRCm38)	missense	probably benign	0.00
effervescence	UTSW	11	84,262,474 (GRCm38)	missense	probably benign	0.41
fizz	UTSW	11	84,245,856 (GRCm38)	missense	probably damaging	0.98
greenhouse	UTSW	11	84,338,356 (GRCm38)	missense	probably damaging	1.00
Serene	UTSW	11	84,311,409 (GRCm38)	splice site	probably null
Tranquil	UTSW	11	84,280,461 (GRCm38)	missense	probably damaging	1.00
vitamin	UTSW	11	84,280,435 (GRCm38)	missense	possibly damaging	0.78
ANU05:Acaca	UTSW	11	84,315,852 (GRCm38)	missense	probably damaging	1.00
R0385:Acaca	UTSW	11	84,231,748 (GRCm38)	missense	probably benign	0.01
R0518:Acaca	UTSW	11	84,290,286 (GRCm38)	critical splice acceptor site	probably null
R0536:Acaca	UTSW	11	84,280,516 (GRCm38)	splice site	probably benign
R0962:Acaca	UTSW	11	84,311,303 (GRCm38)	missense	probably damaging	1.00
R0968:Acaca	UTSW	11	84,239,033 (GRCm38)	nonsense	probably null
R1123:Acaca	UTSW	11	84,264,080 (GRCm38)	missense	probably benign	0.09
R1452:Acaca	UTSW	11	84,295,059 (GRCm38)	splice site	probably benign
R1478:Acaca	UTSW	11	84,372,627 (GRCm38)	missense	probably damaging	1.00
R1500:Acaca	UTSW	11	84,293,984 (GRCm38)	missense	probably benign	0.00
R1512:Acaca	UTSW	11	84,195,469 (GRCm38)	missense	probably benign	0.00
R1657:Acaca	UTSW	11	84,264,084 (GRCm38)	missense	probably benign	0.09
R1681:Acaca	UTSW	11	84,226,185 (GRCm38)	missense	probably damaging	1.00
R1682:Acaca	UTSW	11	84,392,217 (GRCm38)	missense	probably benign	0.23
R1688:Acaca	UTSW	11	84,238,896 (GRCm38)	missense	probably damaging	1.00
R1755:Acaca	UTSW	11	84,276,564 (GRCm38)	frame shift	probably null
R1775:Acaca	UTSW	11	84,300,422 (GRCm38)	missense	possibly damaging	0.56
R1793:Acaca	UTSW	11	84,338,393 (GRCm38)	missense	probably damaging	1.00
R1793:Acaca	UTSW	11	84,315,969 (GRCm38)	missense	probably damaging	0.98
R1855:Acaca	UTSW	11	84,371,554 (GRCm38)	missense	probably damaging	0.96
R1881:Acaca	UTSW	11	84,300,471 (GRCm38)	splice site	probably benign
R1881:Acaca	UTSW	11	84,270,387 (GRCm38)	nonsense	probably null
R1989:Acaca	UTSW	11	84,262,529 (GRCm38)	missense	probably damaging	0.98
R2147:Acaca	UTSW	11	84,276,536 (GRCm38)	missense	probably benign	0.03
R2215:Acaca	UTSW	11	84,363,793 (GRCm38)	missense	probably damaging	1.00
R2238:Acaca	UTSW	11	84,391,505 (GRCm38)	splice site	probably benign
R2252:Acaca	UTSW	11	84,371,532 (GRCm38)	missense	probably damaging	0.99
R2316:Acaca	UTSW	11	84,294,983 (GRCm38)	missense	possibly damaging	0.69
R2316:Acaca	UTSW	11	84,264,080 (GRCm38)	missense	probably benign	0.16
R2337:Acaca	UTSW	11	84,257,197 (GRCm38)	missense	possibly damaging	0.93
R2697:Acaca	UTSW	11	84,364,413 (GRCm38)	missense	probably damaging	1.00
R3551:Acaca	UTSW	11	84,261,624 (GRCm38)	missense	probably damaging	1.00
R3552:Acaca	UTSW	11	84,261,624 (GRCm38)	missense	probably damaging	1.00
R3748:Acaca	UTSW	11	84,311,409 (GRCm38)	splice site	probably null
R3844:Acaca	UTSW	11	84,364,413 (GRCm38)	missense	probably damaging	1.00
R3873:Acaca	UTSW	11	84,312,721 (GRCm38)	unclassified	probably benign
R4152:Acaca	UTSW	11	84,292,926 (GRCm38)	missense	possibly damaging	0.88
R4406:Acaca	UTSW	11	84,280,449 (GRCm38)	missense	probably benign	0.35
R4448:Acaca	UTSW	11	84,262,492 (GRCm38)	missense	probably damaging	1.00
R4642:Acaca	UTSW	11	84,280,461 (GRCm38)	missense	probably damaging	1.00
R4696:Acaca	UTSW	11	84,280,435 (GRCm38)	missense	possibly damaging	0.78
R4707:Acaca	UTSW	11	84,312,854 (GRCm38)	missense	probably damaging	0.96
R4710:Acaca	UTSW	11	84,392,337 (GRCm38)	missense	possibly damaging	0.84
R4775:Acaca	UTSW	11	84,243,339 (GRCm38)	missense	probably damaging	1.00
R4821:Acaca	UTSW	11	84,294,987 (GRCm38)	missense	possibly damaging	0.69
R4883:Acaca	UTSW	11	84,251,290 (GRCm38)	missense	probably benign	0.01
R4988:Acaca	UTSW	11	84,263,295 (GRCm38)	missense	probably damaging	1.00
R5034:Acaca	UTSW	11	84,245,264 (GRCm38)	missense	probably benign	0.00
R5255:Acaca	UTSW	11	84,311,307 (GRCm38)	missense	probably damaging	1.00
R5294:Acaca	UTSW	11	84,391,519 (GRCm38)	missense	probably benign	0.01
R5350:Acaca	UTSW	11	84,215,873 (GRCm38)	missense	probably damaging	0.99
R5437:Acaca	UTSW	11	84,346,820 (GRCm38)	splice site	probably null
R5664:Acaca	UTSW	11	84,243,384 (GRCm38)	missense	probably damaging	1.00
R5665:Acaca	UTSW	11	84,245,294 (GRCm38)	nonsense	probably null
R5959:Acaca	UTSW	11	84,215,966 (GRCm38)	missense	probably damaging	1.00
R6011:Acaca	UTSW	11	84,245,744 (GRCm38)	missense	probably benign	0.44
R6027:Acaca	UTSW	11	84,398,177 (GRCm38)	missense	probably benign
R6246:Acaca	UTSW	11	84,315,970 (GRCm38)	missense	probably benign	0.08
R6313:Acaca	UTSW	11	84,292,929 (GRCm38)	missense	probably benign	0.00
R6450:Acaca	UTSW	11	84,280,468 (GRCm38)	missense	probably damaging	0.98
R6623:Acaca	UTSW	11	84,371,499 (GRCm38)	critical splice acceptor site	probably null
R6736:Acaca	UTSW	11	84,238,838 (GRCm38)	missense	probably benign	0.05
R6752:Acaca	UTSW	11	84,195,483 (GRCm38)	missense	probably benign	0.44
R6807:Acaca	UTSW	11	84,391,530 (GRCm38)	missense	probably benign
R6826:Acaca	UTSW	11	84,195,536 (GRCm38)	missense	probably damaging	1.00
R7035:Acaca	UTSW	11	84,238,943 (GRCm38)	missense	probably damaging	1.00
R7078:Acaca	UTSW	11	84,263,312 (GRCm38)	missense	possibly damaging	0.91
R7088:Acaca	UTSW	11	84,278,957 (GRCm38)	critical splice donor site	probably null
R7201:Acaca	UTSW	11	84,262,474 (GRCm38)	missense	probably benign	0.41
R7261:Acaca	UTSW	11	84,368,700 (GRCm38)	missense	probably damaging	1.00
R7399:Acaca	UTSW	11	84,260,679 (GRCm38)	missense	possibly damaging	0.89
R7421:Acaca	UTSW	11	84,363,736 (GRCm38)	missense	possibly damaging	0.64
R7443:Acaca	UTSW	11	84,315,793 (GRCm38)	missense	probably benign	0.02
R7453:Acaca	UTSW	11	84,245,310 (GRCm38)	missense	probably benign
R7471:Acaca	UTSW	11	84,277,782 (GRCm38)	splice site	probably null
R7519:Acaca	UTSW	11	84,245,856 (GRCm38)	missense	probably damaging	0.98
R7537:Acaca	UTSW	11	84,260,634 (GRCm38)	missense	probably damaging	1.00
R7574:Acaca	UTSW	11	84,261,588 (GRCm38)	missense	probably benign
R7633:Acaca	UTSW	11	84,372,639 (GRCm38)	missense	probably benign	0.26
R7643:Acaca	UTSW	11	84,338,356 (GRCm38)	missense	probably damaging	1.00
R7664:Acaca	UTSW	11	84,245,349 (GRCm38)	missense	probably damaging	1.00
R7675:Acaca	UTSW	11	84,315,916 (GRCm38)	missense	probably benign	0.04
R7676:Acaca	UTSW	11	84,294,987 (GRCm38)	missense	possibly damaging	0.69
R7729:Acaca	UTSW	11	84,371,513 (GRCm38)	missense	probably damaging	0.98
R7867:Acaca	UTSW	11	84,249,524 (GRCm38)	missense	possibly damaging	0.88
R7898:Acaca	UTSW	11	84,364,449 (GRCm38)	critical splice donor site	probably null
R7909:Acaca	UTSW	11	84,245,235 (GRCm38)	missense	possibly damaging	0.56
R7915:Acaca	UTSW	11	84,276,588 (GRCm38)	missense	probably benign
R7956:Acaca	UTSW	11	84,320,580 (GRCm38)	missense	probably damaging	0.98
R8000:Acaca	UTSW	11	84,392,231 (GRCm38)	missense	possibly damaging	0.88
R8038:Acaca	UTSW	11	84,215,904 (GRCm38)	missense	probably damaging	1.00
R8545:Acaca	UTSW	11	84,345,968 (GRCm38)	missense	probably damaging	1.00
R8722:Acaca	UTSW	11	84,338,457 (GRCm38)	missense	possibly damaging	0.85
R9005:Acaca	UTSW	11	84,371,584 (GRCm38)	missense	probably damaging	0.99
R9397:Acaca	UTSW	11	84,368,725 (GRCm38)	missense	probably damaging	1.00
R9489:Acaca	UTSW	11	84,293,016 (GRCm38)	missense	probably benign	0.01
R9540:Acaca	UTSW	11	84,243,411 (GRCm38)	missense	probably damaging	1.00
R9593:Acaca	UTSW	11	84,380,513 (GRCm38)	nonsense	probably null
R9605:Acaca	UTSW	11	84,293,016 (GRCm38)	missense	probably benign	0.01
R9634:Acaca	UTSW	11	84,293,990 (GRCm38)	missense	probably benign	0.00
R9720:Acaca	UTSW	11	84,263,357 (GRCm38)	missense	probably damaging	1.00
RF014:Acaca	UTSW	11	84,231,724 (GRCm38)	missense	probably benign	0.01
X0027:Acaca	UTSW	11	84,292,895 (GRCm38)	missense	probably benign	0.01
X0060:Acaca	UTSW	11	84,264,104 (GRCm38)	missense	probably benign
X0067:Acaca	UTSW	11	84,368,737 (GRCm38)	nonsense	probably null
Z1176:Acaca	UTSW	11	84,260,720 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAAGGTGTTCGGGCATGTCC -3'
(R):5'- ATACCCACCTTTGTGACCAGATC -3'

Sequencing Primer
(F):5'- TGAAACAGGGTTTAATCCTCCTACC -3'
(R):5'- CACCTTTGTGACCAGATCAGAGTG -3'

Posted On

2022-01-20