Mutagenetix > Incidental Mutation

Incidental Mutation 'R9130:Zfp641'

693581

Institutional Source

Beutler Lab

Gene Symbol

Zfp641

Ensembl Gene

ENSMUSG00000022987

Gene Name

zinc finger protein 641

Synonyms

9930016F01Rik

MMRRC Submission

068928-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9130 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

98183466-98194042 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 98186732 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 297 (Q297L)

Ref Sequence

ENSEMBL: ENSMUSP00000133212 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023722] [ENSMUST00000169721]

AlphaFold

Q8BZ34

Predicted Effect

possibly damaging
Transcript: ENSMUST00000023722
AA Change: Q283L

PolyPhen 2 Score 0.462 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000023722
Gene: ENSMUSG00000022987
AA Change: Q283L

Domain	Start	End	E-Value	Type
KRAB	91	142	2.16e-10	SMART
ZnF_C2H2	236	258	1.58e-3	SMART
ZnF_C2H2	264	286	1.67e-2	SMART
ZnF_C2H2	292	314	1.33e-1	SMART
ZnF_C2H2	342	364	8.94e-3	SMART
ZnF_C2H2	370	392	3.83e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169721
AA Change: Q297L

PolyPhen 2 Score 0.117 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000133212
Gene: ENSMUSG00000022987
AA Change: Q297L

Domain	Start	End	E-Value	Type
low complexity region	83	89	N/A	INTRINSIC
KRAB	95	156	2.56e-21	SMART
ZnF_C2H2	250	272	1.58e-3	SMART
ZnF_C2H2	278	300	1.67e-2	SMART
ZnF_C2H2	306	328	1.33e-1	SMART
ZnF_C2H2	356	378	8.94e-3	SMART
ZnF_C2H2	384	406	3.83e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

99% (87/88)

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abr	A	G	11: 76,342,753 (GRCm39)	C585R	possibly damaging	Het
Acaca	T	C	11: 84,202,145 (GRCm39)	L1380S	probably damaging	Het
Aifm2	A	C	10: 61,563,505 (GRCm39)	Q125P	probably null	Het
Ak5	A	T	3: 152,178,569 (GRCm39)	L482*	probably null	Het
Aldh3a2	T	C	11: 61,139,758 (GRCm39)	Q458R	probably benign	Het
Ank2	G	A	3: 126,810,565 (GRCm39)	T524I		Het
Apoc3	A	T	9: 46,146,481 (GRCm39)	S25T	unknown	Het
Ash1l	A	T	3: 88,965,848 (GRCm39)	R2417*	probably null	Het
Bbs12	A	G	3: 37,373,205 (GRCm39)		probably benign	Het
Birc6	T	A	17: 74,919,146 (GRCm39)	I1992N		Het
Brd9	G	A	13: 74,092,906 (GRCm39)	V299M	probably damaging	Het
Brwd1	A	T	16: 95,866,130 (GRCm39)	N217K	probably damaging	Het
C3	T	C	17: 57,518,678 (GRCm39)	Y1235C	probably damaging	Het
Cacna1c	T	C	6: 118,590,907 (GRCm39)	Y1538C		Het
Ccdc172	T	A	19: 58,525,779 (GRCm39)	H212Q	possibly damaging	Het
Cdc6	A	G	11: 98,802,999 (GRCm39)	D313G	probably damaging	Het
Cyp2e1	T	C	7: 140,353,022 (GRCm39)	V353A	probably damaging	Het
Dlg2	G	T	7: 92,080,258 (GRCm39)	V711F	probably damaging	Het
Dmac2	T	C	7: 25,320,448 (GRCm39)	F49S	probably damaging	Het
Dnah7b	A	G	1: 46,173,674 (GRCm39)	K660E	probably benign	Het
Dusp8	A	T	7: 141,642,155 (GRCm39)	S106T	probably benign	Het
Dynll1	T	A	5: 115,438,604 (GRCm39)	I34F	probably benign	Het
Epb41l4b	A	G	4: 57,103,447 (GRCm39)	F130L	possibly damaging	Het
Erc2	T	C	14: 27,751,418 (GRCm39)	Y705H	probably benign	Het
Exosc8	T	A	3: 54,638,503 (GRCm39)	L160F	probably damaging	Het
F5	A	G	1: 164,001,830 (GRCm39)	T178A	probably benign	Het
F7	C	A	8: 13,085,059 (GRCm39)	P362T	probably damaging	Het
Fkbp3	T	C	12: 65,112,567 (GRCm39)	K156E	possibly damaging	Het
Folh1	G	A	7: 86,368,913 (GRCm39)	T738I	probably benign	Het
Gatad2b	C	T	3: 90,255,936 (GRCm39)	A134V	probably benign	Het
Glud1	T	A	14: 34,057,349 (GRCm39)	W338R		Het
Gm21149	T	C	5: 15,680,261 (GRCm39)	K62E	possibly damaging	Het
Gm32742	A	G	9: 51,050,049 (GRCm39)	Y1517H	probably damaging	Het
Grik5	G	A	7: 24,767,429 (GRCm39)		probably benign	Het
Gse1	A	T	8: 121,295,052 (GRCm39)	E391V	unknown	Het
Gxylt2	C	T	6: 100,710,329 (GRCm39)	Q157*	probably null	Het
Hbq1b	C	T	11: 32,237,092 (GRCm39)	T27I	probably damaging	Het
Hoxd13	T	A	2: 74,499,382 (GRCm39)	D243E	probably benign	Het
Hsd3b3	T	A	3: 98,651,211 (GRCm39)	I80F	possibly damaging	Het
Hsf2bp	A	T	17: 32,230,082 (GRCm39)		probably benign	Het
Itch	G	A	2: 155,052,045 (GRCm39)		probably benign	Het
Kat14	T	C	2: 144,215,742 (GRCm39)	F76L	probably benign	Het
Kdm6b	T	C	11: 69,295,424 (GRCm39)	T948A	unknown	Het
Kmt2c	A	T	5: 25,516,102 (GRCm39)	H2580Q	probably benign	Het
Lrp1	T	C	10: 127,382,281 (GRCm39)	T3727A	probably benign	Het
Lrrc8a	A	G	2: 30,147,042 (GRCm39)	I619V	possibly damaging	Het
Mansc1	C	A	6: 134,586,951 (GRCm39)	G409W	probably damaging	Het
Map3k11	C	A	19: 5,746,038 (GRCm39)	L418I	possibly damaging	Het
Mettl4	T	A	17: 95,042,913 (GRCm39)	I308L	possibly damaging	Het
Myo9a	A	G	9: 59,739,514 (GRCm39)	D742G	probably damaging	Het
Mypn	T	C	10: 63,028,652 (GRCm39)	Q137R	probably benign	Het
Neurod4	A	T	10: 130,106,427 (GRCm39)	Y282*	probably null	Het
Nup210	A	G	6: 91,020,799 (GRCm39)	F965S	probably benign	Het
Oplah	T	C	15: 76,185,098 (GRCm39)	T872A	possibly damaging	Het
Or1ab2	G	T	8: 72,863,697 (GRCm39)	G96C	probably damaging	Het
Or4b12	A	G	2: 90,096,358 (GRCm39)	C139R	probably damaging	Het
Or5m8	T	G	2: 85,822,819 (GRCm39)	Y219*	probably null	Het
Or6c68	A	G	10: 129,157,897 (GRCm39)	N135S	probably benign	Het
Pkn2	A	T	3: 142,515,245 (GRCm39)	D696E	possibly damaging	Het
Pla2r1	A	T	2: 60,325,729 (GRCm39)		probably benign	Het
Plpp1	A	T	13: 112,988,038 (GRCm39)	I54L		Het
Ppp4r2	T	G	6: 100,842,113 (GRCm39)	N191K	probably damaging	Het
Ptpdc1	G	T	13: 48,739,655 (GRCm39)	P592Q	probably benign	Het
Qrich2	A	T	11: 116,347,692 (GRCm39)	L1044*	probably null	Het
Rb1cc1	A	G	1: 6,315,109 (GRCm39)	I421V	probably damaging	Het
Ripor3	A	T	2: 167,823,267 (GRCm39)	C881*	probably null	Het
Rreb1	T	C	13: 38,114,282 (GRCm39)	F547S	probably benign	Het
Scel	T	C	14: 103,770,746 (GRCm39)	V60A	probably benign	Het
Shank3	G	A	15: 89,442,419 (GRCm39)	A1771T	probably benign	Het
Slc6a20a	A	G	9: 123,469,631 (GRCm39)		probably null	Het
Slfn5	C	T	11: 82,851,446 (GRCm39)	T581I	probably damaging	Het
Ssh3	A	C	19: 4,314,113 (GRCm39)	V412G	probably damaging	Het
Stx17	T	G	4: 48,159,071 (GRCm39)		probably benign	Het
Sun3	T	G	11: 8,968,170 (GRCm39)	T274P	probably benign	Het
Tm9sf1	T	C	14: 55,875,464 (GRCm39)	T427A	probably damaging	Het
Tmem114	T	A	16: 8,229,983 (GRCm39)	I140F		Het
Tmem40	T	C	6: 115,710,980 (GRCm39)	R167G	possibly damaging	Het
Top3a	C	A	11: 60,641,401 (GRCm39)		probably null	Het
Trafd1	A	G	5: 121,516,573 (GRCm39)	V210A	probably benign	Het
Trim66	T	C	7: 109,076,896 (GRCm39)	I348V	possibly damaging	Het
Ttn	G	A	2: 76,679,172 (GRCm39)	A10850V	unknown	Het
Unc80	G	A	1: 66,677,244 (GRCm39)	A2058T	possibly damaging	Het
Upp2	T	C	2: 58,668,020 (GRCm39)	Y238H	probably damaging	Het
Vmn1r224	T	A	17: 20,640,242 (GRCm39)	I273N	probably damaging	Het
Vps13c	T	C	9: 67,836,805 (GRCm39)	S1768P	probably damaging	Het
Zfp26	G	A	9: 20,348,723 (GRCm39)	H614Y	probably damaging	Het
Zfp263	C	T	16: 3,567,701 (GRCm39)	T672I	probably benign	Het
Zfp944	T	C	17: 22,560,031 (GRCm39)	E25G	probably damaging	Het
Zfp994	T	C	17: 22,418,981 (GRCm39)	E656G	unknown	Het
Zfyve19	A	G	2: 119,045,330 (GRCm39)	D206G	probably damaging	Het

Other mutations in Zfp641

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01639:Zfp641	APN	15	98,189,066 (GRCm39)	missense	possibly damaging	0.85
IGL03092:Zfp641	APN	15	98,188,397 (GRCm39)	missense	probably damaging	1.00
IGL03191:Zfp641	APN	15	98,186,568 (GRCm39)	missense	probably damaging	0.98
R0079:Zfp641	UTSW	15	98,186,970 (GRCm39)	missense	probably benign	0.28
R0243:Zfp641	UTSW	15	98,187,008 (GRCm39)	missense	possibly damaging	0.82
R0487:Zfp641	UTSW	15	98,187,060 (GRCm39)	missense	probably benign
R2092:Zfp641	UTSW	15	98,191,593 (GRCm39)	missense	probably benign
R3415:Zfp641	UTSW	15	98,188,421 (GRCm39)	missense	probably benign	0.28
R4834:Zfp641	UTSW	15	98,191,585 (GRCm39)	missense	probably damaging	1.00
R4851:Zfp641	UTSW	15	98,186,598 (GRCm39)	missense	probably damaging	0.99
R5776:Zfp641	UTSW	15	98,186,891 (GRCm39)	missense	probably damaging	1.00
R6057:Zfp641	UTSW	15	98,190,816 (GRCm39)	missense	probably benign	0.00
R6896:Zfp641	UTSW	15	98,191,684 (GRCm39)	start codon destroyed	probably benign	0.06
R6958:Zfp641	UTSW	15	98,190,832 (GRCm39)	missense	possibly damaging	0.46
R6969:Zfp641	UTSW	15	98,188,448 (GRCm39)	missense	possibly damaging	0.46
R8117:Zfp641	UTSW	15	98,186,856 (GRCm39)	missense	probably damaging	1.00
R8314:Zfp641	UTSW	15	98,188,464 (GRCm39)	missense	probably damaging	0.97
Z1177:Zfp641	UTSW	15	98,186,416 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTGCACACATGGCACTTTGG -3'
(R):5'- GGAACTCCACAGGAATGCTC -3'

Sequencing Primer
(F):5'- ACTTTGGCACGGGAGGC -3'
(R):5'- AGGAGGACACCTTCTCTCG -3'

Posted On

2022-01-20