Mutagenetix > Incidental Mutation

Incidental Mutation 'R9134:Rex2'

693827

Institutional Source

Beutler Lab

Gene Symbol

Rex2

Ensembl Gene

ENSMUSG00000067919

Gene Name

reduced expression 2

Synonyms

Gm13138

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.176) question?

Stock #

R9134 (G1)

Quality Score

91.0077

Status

Not validated

Chromosome

Chromosomal Location

147106307-147145251 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to G at 147142643 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 377 (T377S)

Ref Sequence

ENSEMBL: ENSMUSP00000075179 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075775]

AlphaFold

A2AWF2

Predicted Effect

probably benign
Transcript: ENSMUST00000075775
AA Change: T377S

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000075179
Gene: ENSMUSG00000067919
AA Change: T377S

Domain	Start	End	E-Value	Type
KRAB	28	90	1.46e-16	SMART
low complexity region	235	246	N/A	INTRINSIC
ZnF_C2H2	251	273	7.9e-4	SMART
ZnF_C2H2	279	301	7.9e-4	SMART
ZnF_C2H2	307	329	3.49e-5	SMART
ZnF_C2H2	335	357	1.95e-3	SMART
ZnF_C2H2	363	385	7.9e-4	SMART
ZnF_C2H2	391	413	7.9e-4	SMART
ZnF_C2H2	419	441	3.49e-5	SMART
ZnF_C2H2	447	469	3.89e-3	SMART
ZnF_C2H2	475	497	2.75e-3	SMART
ZnF_C2H2	503	525	3.04e-5	SMART
ZnF_C2H2	531	553	8.34e-3	SMART
ZnF_C2H2	559	581	1.58e-3	SMART
ZnF_C2H2	587	609	1.22e-4	SMART
ZnF_C2H2	615	637	1.28e-3	SMART
ZnF_C2H2	643	665	3.11e-2	SMART
low complexity region	669	685	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

95% (54/57)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ap1m1	A	G	8: 72,993,913 (GRCm39)		probably benign	Het
Arhgdia	A	T	11: 120,470,392 (GRCm39)	I122N	probably damaging	Het
Atad2b	T	A	12: 5,060,351 (GRCm39)	H915Q	probably benign	Het
Atad5	G	A	11: 80,023,931 (GRCm39)	R1681K	probably benign	Het
Bpifb9b	T	C	2: 154,151,441 (GRCm39)	V54A	probably benign	Het
Cby3	A	C	11: 50,250,153 (GRCm39)	T25P	probably damaging	Het
Ccdc138	T	G	10: 58,374,102 (GRCm39)	L374R	probably damaging	Het
Cd84	T	A	1: 171,679,413 (GRCm39)	N30K	probably damaging	Het
Cdk20	T	A	13: 64,580,906 (GRCm39)		probably null	Het
Chd9	A	G	8: 91,659,754 (GRCm39)	H238R	unknown	Het
Cplane1	T	C	15: 8,228,716 (GRCm39)	V929A	probably damaging	Het
Csl	T	A	10: 99,594,237 (GRCm39)	H276L	probably damaging	Het
Dchs1	A	G	7: 105,404,910 (GRCm39)	L2544P	probably damaging	Het
Dnah17	T	C	11: 117,978,972 (GRCm39)	T1807A	probably damaging	Het
Dph7	C	T	2: 24,861,720 (GRCm39)	H378Y	probably benign	Het
Enkur	T	C	2: 21,185,779 (GRCm39)	I249V	probably benign	Het
Fam117a	C	A	11: 95,271,745 (GRCm39)	S439*	probably null	Het
Fam184a	T	G	10: 53,573,344 (GRCm39)	K345N	probably damaging	Het
Fam228a	T	C	12: 4,765,686 (GRCm39)	R255G	probably benign	Het
Fpr-rs7	A	T	17: 20,334,325 (GRCm39)	M55K	probably damaging	Het
Frem2	A	G	3: 53,562,321 (GRCm39)	Y729H	probably damaging	Het
Herc2	A	G	7: 55,832,177 (GRCm39)	T2989A	probably damaging	Het
Hspb8	A	G	5: 116,547,547 (GRCm39)	L145P	probably damaging	Het
Iars1	T	A	13: 49,855,323 (GRCm39)	V250E	probably benign	Het
Ide	G	T	19: 37,273,561 (GRCm39)		probably benign	Het
Ino80c	T	C	18: 24,254,765 (GRCm39)	S32G	probably benign	Het
Insr	G	A	8: 3,308,413 (GRCm39)	R208W	probably damaging	Het
Ints1	G	A	5: 139,743,351 (GRCm39)	R1674W	probably benign	Het
Itsn1	G	A	16: 91,666,514 (GRCm39)	V1153M	unknown	Het
Krt1	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	15: 101,758,813 (GRCm39)		probably benign	Het
Lrrc41	C	A	4: 115,945,782 (GRCm39)	Q166K	possibly damaging	Het
Lrriq3	T	C	3: 154,820,183 (GRCm39)		probably null	Het
Mndal	T	C	1: 173,699,096 (GRCm39)	I190V	unknown	Het
Nisch	TCTGCTGC	TCTGCTGCTGC	14: 30,896,637 (GRCm39)		probably benign	Het
Nme4	G	T	17: 26,314,389 (GRCm39)	A13E	probably benign	Het
Or14c39	C	A	7: 86,344,588 (GRCm39)	S308*	probably null	Het
Or3a1	A	G	11: 74,225,670 (GRCm39)	I129T	probably damaging	Het
Or5b114-ps1	G	T	19: 13,352,613 (GRCm39)	A96S	probably damaging	Het
Pcmt1	A	T	10: 7,520,207 (GRCm39)		probably benign	Het
Pde4c	G	T	8: 71,201,160 (GRCm39)	V493F	probably damaging	Het
Pde8a	C	T	7: 80,982,619 (GRCm39)	T746I	probably damaging	Het
Pira13	T	C	7: 3,825,182 (GRCm39)	S487G		Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 110,350,827 (GRCm39)		probably benign	Het
Prr23a1	T	C	9: 98,724,935 (GRCm39)	L99P		Het
Robo2	A	G	16: 73,703,738 (GRCm39)	F50L		Het
Srgap1	A	G	10: 121,883,127 (GRCm39)		probably benign	Het
Syt3	A	G	7: 44,042,791 (GRCm39)	N358D	possibly damaging	Het
Tfec	T	A	6: 16,835,326 (GRCm39)	T151S	probably damaging	Het
Tll1	A	T	8: 64,469,201 (GRCm39)	I974N	possibly damaging	Het
Tmc2	T	C	2: 130,074,321 (GRCm39)	V338A	probably benign	Het
Trappc6b	T	A	12: 59,097,160 (GRCm39)	D54V	probably damaging	Het
Trav6-2	T	A	14: 52,905,109 (GRCm39)	C43*	probably null	Het
Ubr4	T	A	4: 139,127,755 (GRCm39)		probably null	Het
Vmn1r2	T	A	4: 3,172,884 (GRCm39)	W268R	probably damaging	Het
Vmn2r120	A	G	17: 57,832,093 (GRCm39)	L232S	probably damaging	Het
Vps26b	T	C	9: 26,921,225 (GRCm39)	T325A	probably benign	Het
Vwa3a	T	C	7: 120,377,659 (GRCm39)	V438A	probably damaging	Het
Wdcp	C	A	12: 4,901,533 (GRCm39)	S463*	probably null	Het
Xkr4	C	T	1: 3,740,860 (GRCm39)	G238S	probably benign	Het
Zranb1	A	G	7: 132,551,886 (GRCm39)	N179S	probably benign	Het

Other mutations in Rex2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R4080:Rex2	UTSW	4	147,143,154 (GRCm39)	missense	probably benign
R5075:Rex2	UTSW	4	147,142,151 (GRCm39)	missense	possibly damaging	0.72
R5294:Rex2	UTSW	4	147,142,442 (GRCm39)	missense	probably benign	0.07
R6217:Rex2	UTSW	4	147,141,931 (GRCm39)	missense	possibly damaging	0.77
R6298:Rex2	UTSW	4	147,141,972 (GRCm39)	nonsense	probably null
R6302:Rex2	UTSW	4	147,142,451 (GRCm39)	missense	possibly damaging	0.95
R6614:Rex2	UTSW	4	147,137,018 (GRCm39)	missense	probably benign	0.08
R7572:Rex2	UTSW	4	147,142,329 (GRCm39)	missense	probably damaging	0.98
R7827:Rex2	UTSW	4	147,142,547 (GRCm39)	missense	possibly damaging	0.95
R9792:Rex2	UTSW	4	147,142,039 (GRCm39)	missense	probably damaging	0.99
R9793:Rex2	UTSW	4	147,142,039 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GTGAATGCGACAAATGCTTTACTGA -3'
(R):5'- TGTCACACTCACTGCATTTGTA -3'

Sequencing Primer
(F):5'- GCTTTACTGACAAAGGCAGTCTG -3'
(R):5'- GATGAACTCTCAGACTGCCTTTG -3'

Posted On

2022-01-20