Mutagenetix > Incidental Mutation

Incidental Mutation 'R9135:Mrgprb1'

693926

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb1

Ensembl Gene

ENSMUSG00000070547

Gene Name

MAS-related GPR, member B1

Synonyms

MrgB1

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R9135 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

48444113-48456342 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 48447298 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 289 (I289F)

Ref Sequence

ENSEMBL: ENSMUSP00000091946 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094384] [ENSMUST00000188095] [ENSMUST00000188918]

AlphaFold

Q3UG61

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094384
AA Change: I289F

PolyPhen 2 Score 0.952 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000091946
Gene: ENSMUSG00000070547
AA Change: I289F

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	50	227	5.5e-11	PFAM
Pfam:7tm_1	59	290	4.3e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188095

Predicted Effect

probably benign
Transcript: ENSMUST00000188918

SMART Domains

Protein: ENSMUSP00000140432
Gene: ENSMUSG00000070547

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	23	84	3e-6	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110008L16Rik	T	A	12: 55,379,404 (GRCm38)	L513Q	probably damaging	Het
Abcb9	T	C	5: 124,090,113 (GRCm38)	T22A	possibly damaging	Het
Abi3bp	A	G	16: 56,596,810 (GRCm38)	D419G	probably benign	Het
Acoxl	A	T	2: 127,854,771 (GRCm38)		probably benign	Het
Acvr1	T	C	2: 58,462,971 (GRCm38)	T326A	possibly damaging	Het
Adgra2	T	C	8: 27,120,951 (GRCm38)	L976P	probably damaging	Het
Adora2b	A	G	11: 62,265,060 (GRCm38)		probably null	Het
Ak1	A	T	2: 32,631,170 (GRCm38)	Q117L	probably damaging	Het
Akr1c14	T	A	13: 4,078,029 (GRCm38)	D143E	probably damaging	Het
Ap4e1	A	T	2: 127,019,322 (GRCm38)	H127L	probably damaging	Het
Asxl3	T	A	18: 22,516,613 (GRCm38)	I553N	probably damaging	Het
Asxl3	T	A	18: 22,524,424 (GRCm38)	N1830K	possibly damaging	Het
Atp11a	G	A	8: 12,813,144 (GRCm38)	A108T	probably damaging	Het
Bag6	A	G	17: 35,143,461 (GRCm38)	T582A	unknown	Het
Bbs9	T	C	9: 22,578,709 (GRCm38)	S259P	probably damaging	Het
Bean1	CT	C	8: 104,182,032 (GRCm38)		probably null	Het
Blvrb	T	C	7: 27,465,785 (GRCm38)	S177P	probably damaging	Het
C1s2	T	A	6: 124,625,683 (GRCm38)	D523V	probably benign	Het
Cacna2d1	C	T	5: 16,353,852 (GRCm38)	T736I	probably damaging	Het
Card9	T	C	2: 26,352,385 (GRCm38)	N513S	probably benign	Het
Casp2	A	G	6: 42,268,948 (GRCm38)	T180A	probably benign	Het
Cenpf	A	T	1: 189,672,549 (GRCm38)	L361Q	probably damaging	Het
Cnbd2	T	C	2: 156,375,568 (GRCm38)	I659T	probably damaging	Het
Cped1	A	T	6: 21,987,013 (GRCm38)	D68V	probably damaging	Het
Cxcr1	T	C	1: 74,191,940 (GRCm38)	I308V	probably benign	Het
Dad1	A	G	14: 54,253,706 (GRCm38)	L46P	probably damaging	Het
Defb23	G	A	2: 152,459,502 (GRCm38)	A50V	probably benign	Het
Dhx33	C	T	11: 70,987,166 (GRCm38)	A684T	probably damaging	Het
Epb41l4a	T	A	18: 33,832,676 (GRCm38)	N366I	probably benign	Het
Erich3	A	G	3: 154,762,275 (GRCm38)	E788G		Het
Fam241b	T	C	10: 62,109,113 (GRCm38)	N35S	probably benign	Het
Fbxo24	T	A	5: 137,624,264 (GRCm38)	Q80L	probably benign	Het
Foxk1	T	G	5: 142,448,742 (GRCm38)	F254V	probably benign	Het
Gm10801	C	CGTG	2: 98,663,807 (GRCm38)		probably null	Het
Gm12886	T	C	4: 121,417,546 (GRCm38)	I46V	probably benign	Het
Gm20834	G	T	Y: 10,323,354 (GRCm38)	H27Q	probably damaging	Het
Gm2888	A	G	14: 3,032,106 (GRCm38)	N138S	probably damaging	Het
Gm5773	T	C	3: 93,773,872 (GRCm38)	C284R	possibly damaging	Het
Gprin3	A	T	6: 59,353,288 (GRCm38)	M678K	probably benign	Het
Grm7	T	C	6: 111,495,768 (GRCm38)	V856A	probably benign	Het
Il18rap	C	T	1: 40,543,017 (GRCm38)	T366M	probably benign	Het
Immt	C	T	6: 71,874,419 (GRCm38)	T588I	probably damaging	Het
Ints1	G	C	5: 139,751,946 (GRCm38)	N2145K	possibly damaging	Het
Jade2	T	C	11: 51,825,124 (GRCm38)	D418G	probably benign	Het
Kdf1	T	C	4: 133,528,829 (GRCm38)	Y286H	probably damaging	Het
Kif18a	A	G	2: 109,341,161 (GRCm38)	Y861C	possibly damaging	Het
Lama2	T	A	10: 27,422,519 (GRCm38)	I194F	probably damaging	Het
Lhx8	A	G	3: 154,328,426 (GRCm38)	S33P	probably benign	Het
Lmo7	T	C	14: 101,880,861 (GRCm38)	I174T	probably damaging	Het
Lrfn2	A	T	17: 49,069,948 (GRCm38)	D19V	possibly damaging	Het
Lrrk1	A	T	7: 66,278,609 (GRCm38)	I1257N	probably damaging	Het
Malrd1	T	A	2: 15,797,705 (GRCm38)	H1076Q	unknown	Het
Map3k14	A	C	11: 103,237,538 (GRCm38)	M419R	probably damaging	Het
Mcm10	G	A	2: 5,006,561 (GRCm38)	R200C	probably benign	Het
Mertk	A	G	2: 128,762,115 (GRCm38)	N390D	probably benign	Het
Mitf	T	A	6: 98,013,719 (GRCm38)	I361N	probably damaging	Het
Muc5ac	A	T	7: 141,798,481 (GRCm38)	D659V	probably damaging	Het
Nox4	G	A	7: 87,376,240 (GRCm38)	R525Q	probably benign	Het
Nphp3	T	G	9: 104,032,015 (GRCm38)	L808R	probably damaging	Het
Olfr1434	T	A	19: 12,283,444 (GRCm38)	L132Q	probably damaging	Het
Olfr1467	T	A	19: 13,365,391 (GRCm38)	S254R	possibly damaging	Het
Olfr20	T	C	11: 73,354,490 (GRCm38)	S246P	probably damaging	Het
Olfr296-ps1	G	A	7: 86,561,948 (GRCm38)	C72Y	unknown	Het
Pagr1a	T	C	7: 127,016,782 (GRCm38)	E47G	probably benign	Het
Papss2	G	T	19: 32,641,364 (GRCm38)	R274L	probably damaging	Het
Pcdhga10	C	A	18: 37,747,907 (GRCm38)	F240L	probably damaging	Het
Pcsk5	T	G	19: 17,586,108 (GRCm38)	E555A		Het
Peg10	C	CTCA	6: 4,756,453 (GRCm38)		probably benign	Het
Pgrmc2	T	A	3: 41,068,664 (GRCm38)	D206V	probably damaging	Het
Pkd1	T	C	17: 24,572,002 (GRCm38)	V1057A		Het
Plekha5	T	C	6: 140,534,513 (GRCm38)	I113T	probably damaging	Het
Prss32	C	T	17: 23,859,225 (GRCm38)	T295I	possibly damaging	Het
Ptprk	G	T	10: 28,580,417 (GRCm38)	V1072F	probably damaging	Het
Rasd2	T	A	8: 75,218,546 (GRCm38)	M1K	probably null	Het
Rhag	T	A	17: 40,828,411 (GRCm38)	M79K	probably damaging	Het
Rnf19a	C	A	15: 36,253,164 (GRCm38)		probably null	Het
Rnf213	G	T	11: 119,408,747 (GRCm38)	G101C		Het
Sap130	T	A	18: 31,647,063 (GRCm38)	D45E	probably benign	Het
Scel	T	A	14: 103,602,190 (GRCm38)	F510I	probably benign	Het
Sertad2	A	T	11: 20,648,425 (GRCm38)	E207V	probably benign	Het
Shoc2	T	A	19: 53,987,879 (GRCm38)	S67T	probably benign	Het
Slc22a3	T	C	17: 12,426,732 (GRCm38)	I441V	possibly damaging	Het
Slfn5	A	G	11: 82,960,677 (GRCm38)	D600G	probably benign	Het
Smim7	G	A	8: 72,570,913 (GRCm38)	T10M	probably damaging	Het
Sntb2	A	T	8: 106,936,199 (GRCm38)	Q133L	possibly damaging	Het
Steap3	A	C	1: 120,234,318 (GRCm38)	M408R	probably benign	Het
Supt4a	T	C	11: 87,742,798 (GRCm38)	I60T	probably benign	Het
Swt1	A	C	1: 151,368,488 (GRCm38)	C899G	possibly damaging	Het
Taok3	A	G	5: 117,206,679 (GRCm38)	E157G	probably damaging	Het
Taok3	T	C	5: 117,241,103 (GRCm38)	V401A	probably benign	Het
Taok3	C	T	5: 117,241,180 (GRCm38)	L427F	possibly damaging	Het
Tcf25	A	G	8: 123,381,443 (GRCm38)	E79G	probably benign	Het
Tigit	G	A	16: 43,659,513 (GRCm38)	L140F	possibly damaging	Het
Tmem143	G	T	7: 45,897,378 (GRCm38)		probably benign	Het
Tmem151b	C	T	17: 45,545,429 (GRCm38)	V362M	possibly damaging	Het
Tmem230	A	G	2: 132,244,069 (GRCm38)	F58L	probably benign	Het
Trim25	T	C	11: 89,009,162 (GRCm38)	M245T	probably benign	Het
Trim36	A	C	18: 46,169,343 (GRCm38)	V546G	probably benign	Het
Triml1	T	C	8: 43,130,587 (GRCm38)	N326D	probably damaging	Het
Trp53bp2	T	C	1: 182,458,763 (GRCm38)	V1056A	probably damaging	Het
Trpa1	C	A	1: 14,882,211 (GRCm38)	M865I	probably damaging	Het
Ttbk1	A	T	17: 46,479,206 (GRCm38)	Y104*	probably null	Het
Tubg1	T	A	11: 101,123,431 (GRCm38)	H139Q	probably damaging	Het
Ufsp2	A	G	8: 45,994,013 (GRCm38)		probably null	Het
Uhrf1bp1	T	A	17: 27,885,928 (GRCm38)	L532*	probably null	Het
Usp47	A	T	7: 112,053,224 (GRCm38)	N36I	probably benign	Het
Vmn1r32	G	A	6: 66,553,136 (GRCm38)	R219*	probably null	Het
Vmn2r59	G	C	7: 42,043,703 (GRCm38)	A491G		Het
Vmn2r59	G	A	7: 42,043,701 (GRCm38)	P492S	probably benign	Het
Wdr63	T	C	3: 146,066,834 (GRCm38)	T486A	probably benign	Het
Wdr76	T	C	2: 121,534,111 (GRCm38)	Y299H	probably damaging	Het
Wnt5a	C	T	14: 28,518,352 (GRCm38)	A153V	probably benign	Het
Zfp202	T	C	9: 40,208,941 (GRCm38)	V226A	possibly damaging	Het
Zfp219	C	T	14: 52,007,141 (GRCm38)	R527H	probably damaging	Het
Zfp408	A	T	2: 91,645,183 (GRCm38)	L542H	probably damaging	Het
Zfyve9	A	T	4: 108,682,189 (GRCm38)	C271*	probably null	Het

Other mutations in Mrgprb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00540:Mrgprb1	APN	7	48,447,543 (GRCm38)	missense	probably damaging	0.99
IGL01141:Mrgprb1	APN	7	48,448,027 (GRCm38)	missense	probably benign	0.36
IGL01393:Mrgprb1	APN	7	48,448,006 (GRCm38)	missense	possibly damaging	0.48
IGL02430:Mrgprb1	APN	7	48,447,661 (GRCm38)	missense	possibly damaging	0.95
IGL02485:Mrgprb1	APN	7	48,447,717 (GRCm38)	missense	possibly damaging	0.88
R0026:Mrgprb1	UTSW	7	48,447,204 (GRCm38)	missense	possibly damaging	0.66
R0051:Mrgprb1	UTSW	7	48,447,214 (GRCm38)	missense	probably benign	0.01
R0789:Mrgprb1	UTSW	7	48,456,184 (GRCm38)	splice site	probably benign
R1223:Mrgprb1	UTSW	7	48,447,687 (GRCm38)	missense	possibly damaging	0.61
R1327:Mrgprb1	UTSW	7	48,447,429 (GRCm38)	missense	possibly damaging	0.87
R1456:Mrgprb1	UTSW	7	48,448,029 (GRCm38)	missense	probably damaging	0.98
R1561:Mrgprb1	UTSW	7	48,447,125 (GRCm38)	splice site	probably null
R1567:Mrgprb1	UTSW	7	48,447,453 (GRCm38)	missense	probably damaging	0.97
R2030:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.83
R2165:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.00
R2885:Mrgprb1	UTSW	7	48,447,721 (GRCm38)	missense	probably damaging	1.00
R3108:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.93
R3919:Mrgprb1	UTSW	7	48,448,081 (GRCm38)	missense	probably benign	0.03
R4021:Mrgprb1	UTSW	7	48,447,123 (GRCm38)	missense	possibly damaging	0.95
R4613:Mrgprb1	UTSW	7	48,447,708 (GRCm38)	missense	possibly damaging	0.91
R4809:Mrgprb1	UTSW	7	48,447,991 (GRCm38)	missense	possibly damaging	0.89
R5249:Mrgprb1	UTSW	7	48,447,477 (GRCm38)	missense	possibly damaging	0.91
R5425:Mrgprb1	UTSW	7	48,447,971 (GRCm38)	missense	possibly damaging	0.81
R5555:Mrgprb1	UTSW	7	48,447,775 (GRCm38)	missense	probably benign	0.06
R5595:Mrgprb1	UTSW	7	48,447,684 (GRCm38)	missense	probably damaging	0.99
R5982:Mrgprb1	UTSW	7	48,447,820 (GRCm38)	missense	probably benign	0.01
R6746:Mrgprb1	UTSW	7	48,447,897 (GRCm38)	missense	possibly damaging	0.82
R7066:Mrgprb1	UTSW	7	48,447,676 (GRCm38)	missense	probably benign	0.27
R7141:Mrgprb1	UTSW	7	48,447,687 (GRCm38)	missense	possibly damaging	0.61
R7633:Mrgprb1	UTSW	7	48,447,583 (GRCm38)	missense	probably benign	0.01
R8072:Mrgprb1	UTSW	7	48,448,147 (GRCm38)	nonsense	probably null
R8080:Mrgprb1	UTSW	7	48,446,910 (GRCm38)	splice site	probably null
R8112:Mrgprb1	UTSW	7	48,447,934 (GRCm38)	missense	probably damaging	0.97
R8493:Mrgprb1	UTSW	7	48,447,573 (GRCm38)	missense	probably damaging	0.99
R8817:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ATCAAAGCAGCTCTCAGTCC -3'
(R):5'- TGGCCTTGGTGCTTACCATC -3'

Sequencing Primer
(F):5'- GCTCTCAGTCCCTTCCAGACAG -3'
(R):5'- TTACACAAGGTTCCTGTGACCAG -3'

Posted On

2022-01-20