Mutagenetix > Incidental Mutation

Incidental Mutation 'R9135:Abi3bp'

693969

Institutional Source

Beutler Lab

Gene Symbol

Abi3bp

Ensembl Gene

ENSMUSG00000035258

Gene Name

ABI family member 3 binding protein

Synonyms

D930038M13Rik, TARSH, 5033411B22Rik, eratin

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R9135 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

56298241-56510498 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 56417173 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 419 (D419G)

Ref Sequence

ENSEMBL: ENSMUSP00000036257 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048471] [ENSMUST00000096012] [ENSMUST00000096013] [ENSMUST00000171000] [ENSMUST00000231781] [ENSMUST00000231832] [ENSMUST00000231870]

AlphaFold

A0A338P6S8

Predicted Effect

probably benign
Transcript: ENSMUST00000048471
AA Change: D419G

PolyPhen 2 Score 0.212 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000036257
Gene: ENSMUSG00000035258
AA Change: D419G

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
FN3	114	203	3.08e-2	SMART
low complexity region	433	446	N/A	INTRINSIC
low complexity region	516	528	N/A	INTRINSIC
low complexity region	579	591	N/A	INTRINSIC
low complexity region	734	747	N/A	INTRINSIC
low complexity region	751	764	N/A	INTRINSIC
FN3	941	1024	6.29e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000096012
AA Change: D419G

PolyPhen 2 Score 0.025 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000093711
Gene: ENSMUSG00000035258
AA Change: D419G

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
FN3	114	203	3.08e-2	SMART
low complexity region	433	446	N/A	INTRINSIC
low complexity region	634	647	N/A	INTRINSIC
low complexity region	651	664	N/A	INTRINSIC
FN3	841	924	6.29e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000096013
AA Change: D419G

PolyPhen 2 Score 0.025 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000093712
Gene: ENSMUSG00000035258
AA Change: D419G

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
FN3	114	203	3.08e-2	SMART
low complexity region	433	446	N/A	INTRINSIC
low complexity region	670	683	N/A	INTRINSIC
low complexity region	687	700	N/A	INTRINSIC
FN3	877	960	6.29e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000171000

SMART Domains

Protein: ENSMUSP00000128818
Gene: ENSMUSG00000035258

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
FN3	114	203	3.08e-2	SMART
low complexity region	464	477	N/A	INTRINSIC
low complexity region	481	494	N/A	INTRINSIC
FN3	671	754	6.29e-8	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000231781
AA Change: D419G

PolyPhen 2 Score 0.706 (Sensitivity: 0.86; Specificity: 0.92)

Predicted Effect

probably benign
Transcript: ENSMUST00000231832

Predicted Effect

probably damaging
Transcript: ENSMUST00000231870
AA Change: D419G

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb9	T	C	5: 124,228,176 (GRCm39)	T22A	possibly damaging	Het
Acoxl	A	T	2: 127,696,691 (GRCm39)		probably benign	Het
Acvr1	T	C	2: 58,352,983 (GRCm39)	T326A	possibly damaging	Het
Adgra2	T	C	8: 27,610,979 (GRCm39)	L976P	probably damaging	Het
Adora2b	A	G	11: 62,155,886 (GRCm39)		probably null	Het
Ak1	A	T	2: 32,521,182 (GRCm39)	Q117L	probably damaging	Het
Akr1c14	T	A	13: 4,128,029 (GRCm39)	D143E	probably damaging	Het
Ap4e1	A	T	2: 126,861,242 (GRCm39)	H127L	probably damaging	Het
Asxl3	T	A	18: 22,649,670 (GRCm39)	I553N	probably damaging	Het
Asxl3	T	A	18: 22,657,481 (GRCm39)	N1830K	possibly damaging	Het
Atp11a	G	A	8: 12,863,144 (GRCm39)	A108T	probably damaging	Het
Bag6	A	G	17: 35,362,437 (GRCm39)	T582A	unknown	Het
Bbs9	T	C	9: 22,490,005 (GRCm39)	S259P	probably damaging	Het
Bean1	CT	C	8: 104,908,664 (GRCm39)		probably null	Het
Bltp3a	T	A	17: 28,104,902 (GRCm39)	L532*	probably null	Het
Blvrb	T	C	7: 27,165,210 (GRCm39)	S177P	probably damaging	Het
C1s2	T	A	6: 124,602,642 (GRCm39)	D523V	probably benign	Het
Cacna2d1	C	T	5: 16,558,850 (GRCm39)	T736I	probably damaging	Het
Card9	T	C	2: 26,242,397 (GRCm39)	N513S	probably benign	Het
Casp2	A	G	6: 42,245,882 (GRCm39)	T180A	probably benign	Het
Cenpf	A	T	1: 189,404,746 (GRCm39)	L361Q	probably damaging	Het
Cnbd2	T	C	2: 156,217,488 (GRCm39)	I659T	probably damaging	Het
Cped1	A	T	6: 21,987,012 (GRCm39)	D68V	probably damaging	Het
Cxcr1	T	C	1: 74,231,099 (GRCm39)	I308V	probably benign	Het
Dad1	A	G	14: 54,491,163 (GRCm39)	L46P	probably damaging	Het
Defb23	G	A	2: 152,301,422 (GRCm39)	A50V	probably benign	Het
Dhx33	C	T	11: 70,877,992 (GRCm39)	A684T	probably damaging	Het
Dnai3	T	C	3: 145,772,589 (GRCm39)	T486A	probably benign	Het
Epb41l4a	T	A	18: 33,965,729 (GRCm39)	N366I	probably benign	Het
Erich3	A	G	3: 154,467,912 (GRCm39)	E788G		Het
Fam241b	T	C	10: 61,944,892 (GRCm39)	N35S	probably benign	Het
Fbxo24	T	A	5: 137,622,526 (GRCm39)	Q80L	probably benign	Het
Foxk1	T	G	5: 142,434,497 (GRCm39)	F254V	probably benign	Het
Gm10801	C	CGTG	2: 98,494,152 (GRCm39)		probably null	Het
Gm12886	T	C	4: 121,274,743 (GRCm39)	I46V	probably benign	Het
Gm20834	G	T	Y: 10,323,354 (GRCm39)	H27Q	probably damaging	Het
Gm2888	A	G	14: 3,032,106 (GRCm38)	N138S	probably damaging	Het
Gm5773	T	C	3: 93,681,179 (GRCm39)	C284R	possibly damaging	Het
Gprin3	A	T	6: 59,330,273 (GRCm39)	M678K	probably benign	Het
Grm7	T	C	6: 111,472,729 (GRCm39)	V856A	probably benign	Het
Il18rap	C	T	1: 40,582,177 (GRCm39)	T366M	probably benign	Het
Immt	C	T	6: 71,851,403 (GRCm39)	T588I	probably damaging	Het
Ints1	G	C	5: 139,737,701 (GRCm39)	N2145K	possibly damaging	Het
Jade2	T	C	11: 51,715,951 (GRCm39)	D418G	probably benign	Het
Kdf1	T	C	4: 133,256,140 (GRCm39)	Y286H	probably damaging	Het
Kif18a	A	G	2: 109,171,506 (GRCm39)	Y861C	possibly damaging	Het
Lama2	T	A	10: 27,298,515 (GRCm39)	I194F	probably damaging	Het
Lhx8	A	G	3: 154,034,063 (GRCm39)	S33P	probably benign	Het
Lmo7	T	C	14: 102,118,297 (GRCm39)	I174T	probably damaging	Het
Lrfn2	A	T	17: 49,376,976 (GRCm39)	D19V	possibly damaging	Het
Lrrk1	A	T	7: 65,928,357 (GRCm39)	I1257N	probably damaging	Het
Malrd1	T	A	2: 15,802,516 (GRCm39)	H1076Q	unknown	Het
Map3k14	A	C	11: 103,128,364 (GRCm39)	M419R	probably damaging	Het
Mcm10	G	A	2: 5,011,372 (GRCm39)	R200C	probably benign	Het
Mertk	A	G	2: 128,604,035 (GRCm39)	N390D	probably benign	Het
Mitf	T	A	6: 97,990,680 (GRCm39)	I361N	probably damaging	Het
Mrgprb1	T	A	7: 48,097,046 (GRCm39)	I289F	possibly damaging	Het
Muc5ac	A	T	7: 141,352,218 (GRCm39)	D659V	probably damaging	Het
Nox4	G	A	7: 87,025,448 (GRCm39)	R525Q	probably benign	Het
Nphp3	T	G	9: 103,909,214 (GRCm39)	L808R	probably damaging	Het
Or14c42-ps1	G	A	7: 86,211,156 (GRCm39)	C72Y	unknown	Het
Or1e1	T	C	11: 73,245,316 (GRCm39)	S246P	probably damaging	Het
Or5an1	T	A	19: 12,260,808 (GRCm39)	L132Q	probably damaging	Het
Or5b113	T	A	19: 13,342,755 (GRCm39)	S254R	possibly damaging	Het
Pagr1a	T	C	7: 126,615,954 (GRCm39)	E47G	probably benign	Het
Papss2	G	T	19: 32,618,764 (GRCm39)	R274L	probably damaging	Het
Pcdhga10	C	A	18: 37,880,960 (GRCm39)	F240L	probably damaging	Het
Pcsk5	T	G	19: 17,563,472 (GRCm39)	E555A		Het
Peg10	C	CTCA	6: 4,756,453 (GRCm39)		probably benign	Het
Pgrmc2	T	A	3: 41,023,099 (GRCm39)	D206V	probably damaging	Het
Pkd1	T	C	17: 24,790,976 (GRCm39)	V1057A		Het
Plekha5	T	C	6: 140,480,239 (GRCm39)	I113T	probably damaging	Het
Prorp	T	A	12: 55,426,189 (GRCm39)	L513Q	probably damaging	Het
Prss32	C	T	17: 24,078,199 (GRCm39)	T295I	possibly damaging	Het
Ptprk	G	T	10: 28,456,413 (GRCm39)	V1072F	probably damaging	Het
Rasd2	T	A	8: 75,945,174 (GRCm39)	M1K	probably null	Het
Rhag	T	A	17: 41,139,302 (GRCm39)	M79K	probably damaging	Het
Rnf19a	C	A	15: 36,253,310 (GRCm39)		probably null	Het
Rnf213	G	T	11: 119,299,573 (GRCm39)	G101C		Het
Sap130	T	A	18: 31,780,116 (GRCm39)	D45E	probably benign	Het
Scel	T	A	14: 103,839,626 (GRCm39)	F510I	probably benign	Het
Sertad2	A	T	11: 20,598,425 (GRCm39)	E207V	probably benign	Het
Shoc2	T	A	19: 53,976,310 (GRCm39)	S67T	probably benign	Het
Slc22a3	T	C	17: 12,645,619 (GRCm39)	I441V	possibly damaging	Het
Slfn5	A	G	11: 82,851,503 (GRCm39)	D600G	probably benign	Het
Smim7	G	A	8: 73,324,757 (GRCm39)	T10M	probably damaging	Het
Sntb2	A	T	8: 107,662,831 (GRCm39)	Q133L	possibly damaging	Het
Steap3	A	C	1: 120,162,048 (GRCm39)	M408R	probably benign	Het
Supt4a	T	C	11: 87,633,624 (GRCm39)	I60T	probably benign	Het
Swt1	A	C	1: 151,244,239 (GRCm39)	C899G	possibly damaging	Het
Taok3	A	G	5: 117,344,744 (GRCm39)	E157G	probably damaging	Het
Taok3	C	T	5: 117,379,245 (GRCm39)	L427F	possibly damaging	Het
Taok3	T	C	5: 117,379,168 (GRCm39)	V401A	probably benign	Het
Tcf25	A	G	8: 124,108,182 (GRCm39)	E79G	probably benign	Het
Tigit	G	A	16: 43,479,876 (GRCm39)	L140F	possibly damaging	Het
Tmem143	G	T	7: 45,546,802 (GRCm39)		probably benign	Het
Tmem151b	C	T	17: 45,856,355 (GRCm39)	V362M	possibly damaging	Het
Tmem230	A	G	2: 132,085,989 (GRCm39)	F58L	probably benign	Het
Trim25	T	C	11: 88,899,988 (GRCm39)	M245T	probably benign	Het
Trim36	A	C	18: 46,302,410 (GRCm39)	V546G	probably benign	Het
Triml1	T	C	8: 43,583,624 (GRCm39)	N326D	probably damaging	Het
Trp53bp2	T	C	1: 182,286,328 (GRCm39)	V1056A	probably damaging	Het
Trpa1	C	A	1: 14,952,435 (GRCm39)	M865I	probably damaging	Het
Ttbk1	A	T	17: 46,790,132 (GRCm39)	Y104*	probably null	Het
Tubg1	T	A	11: 101,014,257 (GRCm39)	H139Q	probably damaging	Het
Ufsp2	A	G	8: 46,447,050 (GRCm39)		probably null	Het
Usp47	A	T	7: 111,652,431 (GRCm39)	N36I	probably benign	Het
Vmn1r32	G	A	6: 66,530,120 (GRCm39)	R219*	probably null	Het
Vmn2r59	G	A	7: 41,693,125 (GRCm39)	P492S	probably benign	Het
Vmn2r59	G	C	7: 41,693,127 (GRCm39)	A491G		Het
Wdr76	T	C	2: 121,364,592 (GRCm39)	Y299H	probably damaging	Het
Wnt5a	C	T	14: 28,240,309 (GRCm39)	A153V	probably benign	Het
Zfp202	T	C	9: 40,120,237 (GRCm39)	V226A	possibly damaging	Het
Zfp219	C	T	14: 52,244,598 (GRCm39)	R527H	probably damaging	Het
Zfp408	A	T	2: 91,475,528 (GRCm39)	L542H	probably damaging	Het
Zfyve9	A	T	4: 108,539,386 (GRCm39)	C271*	probably null	Het

Other mutations in Abi3bp

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00780:Abi3bp	APN	16	56,423,168 (GRCm39)	missense	probably null	0.99
IGL01580:Abi3bp	APN	16	56,495,573 (GRCm39)	missense	probably damaging	1.00
IGL01633:Abi3bp	APN	16	56,498,163 (GRCm39)	missense	probably damaging	1.00
IGL01783:Abi3bp	APN	16	56,353,332 (GRCm39)	critical splice donor site	probably null
IGL01866:Abi3bp	APN	16	56,492,336 (GRCm39)	missense	probably benign	0.19
IGL02022:Abi3bp	APN	16	56,412,999 (GRCm39)	missense	probably damaging	1.00
IGL02086:Abi3bp	APN	16	56,462,930 (GRCm39)	splice site	probably benign
IGL02122:Abi3bp	APN	16	56,507,491 (GRCm39)	splice site	probably benign
IGL02155:Abi3bp	APN	16	56,408,327 (GRCm39)	missense	probably damaging	0.99
IGL02351:Abi3bp	APN	16	56,474,418 (GRCm39)	missense	possibly damaging	0.91
IGL02358:Abi3bp	APN	16	56,474,418 (GRCm39)	missense	possibly damaging	0.91
IGL02418:Abi3bp	APN	16	56,424,479 (GRCm39)	splice site	probably benign
IGL02559:Abi3bp	APN	16	56,507,433 (GRCm39)	nonsense	probably null
IGL02617:Abi3bp	APN	16	56,394,807 (GRCm39)	nonsense	probably null
IGL02810:Abi3bp	APN	16	56,498,138 (GRCm39)	missense	probably damaging	1.00
IGL03057:Abi3bp	APN	16	56,488,754 (GRCm39)	missense	possibly damaging	0.95
IGL03174:Abi3bp	APN	16	56,435,110 (GRCm39)	missense	possibly damaging	0.64
R0389:Abi3bp	UTSW	16	56,491,670 (GRCm39)	missense	possibly damaging	0.79
R0485:Abi3bp	UTSW	16	56,424,375 (GRCm39)	splice site	probably null
R0557:Abi3bp	UTSW	16	56,488,750 (GRCm39)	missense	probably damaging	0.97
R0616:Abi3bp	UTSW	16	56,474,433 (GRCm39)	missense	probably damaging	0.99
R0685:Abi3bp	UTSW	16	56,353,316 (GRCm39)	missense	possibly damaging	0.90
R0783:Abi3bp	UTSW	16	56,415,601 (GRCm39)	critical splice acceptor site	probably null
R0828:Abi3bp	UTSW	16	56,498,193 (GRCm39)	missense	probably damaging	1.00
R0841:Abi3bp	UTSW	16	56,488,639 (GRCm39)	missense	possibly damaging	0.95
R1078:Abi3bp	UTSW	16	56,474,444 (GRCm39)	critical splice donor site	probably null
R1101:Abi3bp	UTSW	16	56,426,521 (GRCm39)	missense	probably damaging	1.00
R1116:Abi3bp	UTSW	16	56,506,792 (GRCm39)	splice site	probably benign
R1145:Abi3bp	UTSW	16	56,488,639 (GRCm39)	missense	possibly damaging	0.95
R1145:Abi3bp	UTSW	16	56,488,639 (GRCm39)	missense	possibly damaging	0.95
R1317:Abi3bp	UTSW	16	56,488,672 (GRCm39)	missense	possibly damaging	0.79
R1384:Abi3bp	UTSW	16	56,394,862 (GRCm39)	missense	probably damaging	1.00
R1460:Abi3bp	UTSW	16	56,382,780 (GRCm39)	missense	probably damaging	0.99
R1730:Abi3bp	UTSW	16	56,488,642 (GRCm39)	missense	possibly damaging	0.62
R1761:Abi3bp	UTSW	16	56,488,672 (GRCm39)	missense	possibly damaging	0.79
R1830:Abi3bp	UTSW	16	56,408,348 (GRCm39)	missense	probably damaging	1.00
R1873:Abi3bp	UTSW	16	56,394,862 (GRCm39)	missense	probably damaging	1.00
R1875:Abi3bp	UTSW	16	56,394,862 (GRCm39)	missense	probably damaging	1.00
R1996:Abi3bp	UTSW	16	56,491,720 (GRCm39)	missense	possibly damaging	0.61
R2018:Abi3bp	UTSW	16	56,498,159 (GRCm39)	missense	probably damaging	1.00
R2019:Abi3bp	UTSW	16	56,498,159 (GRCm39)	missense	probably damaging	1.00
R2035:Abi3bp	UTSW	16	56,480,581 (GRCm39)	missense	probably benign	0.21
R2118:Abi3bp	UTSW	16	56,298,227 (GRCm39)	unclassified	probably benign
R2202:Abi3bp	UTSW	16	56,433,566 (GRCm39)	missense	probably benign	0.06
R2202:Abi3bp	UTSW	16	56,471,088 (GRCm39)	nonsense	probably null
R2203:Abi3bp	UTSW	16	56,433,566 (GRCm39)	missense	probably benign	0.06
R3030:Abi3bp	UTSW	16	56,477,682 (GRCm39)	missense	possibly damaging	0.79
R3952:Abi3bp	UTSW	16	56,424,401 (GRCm39)	missense	possibly damaging	0.88
R4176:Abi3bp	UTSW	16	56,472,563 (GRCm39)	missense	probably damaging	0.96
R4296:Abi3bp	UTSW	16	56,488,673 (GRCm39)	missense	probably benign	0.05
R4301:Abi3bp	UTSW	16	56,377,266 (GRCm39)	missense	probably damaging	1.00
R4354:Abi3bp	UTSW	16	56,353,314 (GRCm39)	missense	probably benign	0.05
R4417:Abi3bp	UTSW	16	56,474,398 (GRCm39)	missense	probably damaging	1.00
R4716:Abi3bp	UTSW	16	56,471,088 (GRCm39)	nonsense	probably null
R4808:Abi3bp	UTSW	16	56,414,879 (GRCm39)	missense	probably damaging	0.96
R4814:Abi3bp	UTSW	16	56,471,116 (GRCm39)	missense	probably benign	0.06
R5016:Abi3bp	UTSW	16	56,491,631 (GRCm39)	missense	probably damaging	0.97
R5290:Abi3bp	UTSW	16	56,462,838 (GRCm39)	splice site	probably null
R5891:Abi3bp	UTSW	16	56,426,496 (GRCm39)	missense	probably damaging	1.00
R5897:Abi3bp	UTSW	16	56,425,032 (GRCm39)	missense	possibly damaging	0.53
R6146:Abi3bp	UTSW	16	56,491,628 (GRCm39)	missense	probably damaging	0.99
R6267:Abi3bp	UTSW	16	56,414,860 (GRCm39)	missense	probably damaging	0.97
R6905:Abi3bp	UTSW	16	56,394,880 (GRCm39)	missense	probably damaging	1.00
R6908:Abi3bp	UTSW	16	56,477,668 (GRCm39)	missense	probably benign	0.01
R6917:Abi3bp	UTSW	16	56,437,684 (GRCm39)	splice site	probably null
R7071:Abi3bp	UTSW	16	56,449,503 (GRCm39)	nonsense	probably null
R7194:Abi3bp	UTSW	16	56,382,734 (GRCm39)	missense	probably damaging	0.99
R7476:Abi3bp	UTSW	16	56,435,109 (GRCm39)	nonsense	probably null
R7554:Abi3bp	UTSW	16	56,438,575 (GRCm39)	splice site	probably null
R7571:Abi3bp	UTSW	16	56,451,345 (GRCm39)	splice site	probably null
R7661:Abi3bp	UTSW	16	56,453,263 (GRCm39)	splice site	probably null
R7662:Abi3bp	UTSW	16	56,437,686 (GRCm39)	splice site	probably null
R7910:Abi3bp	UTSW	16	56,498,105 (GRCm39)	nonsense	probably null
R8121:Abi3bp	UTSW	16	56,452,241 (GRCm39)	missense	unknown
R8781:Abi3bp	UTSW	16	56,426,512 (GRCm39)	missense	probably damaging	0.98
R8790:Abi3bp	UTSW	16	56,495,437 (GRCm39)	missense	probably damaging	1.00
R8828:Abi3bp	UTSW	16	56,507,455 (GRCm39)	missense	probably damaging	1.00
R9094:Abi3bp	UTSW	16	56,456,590 (GRCm39)	missense	probably benign	0.00
R9282:Abi3bp	UTSW	16	56,440,867 (GRCm39)	missense	unknown
R9363:Abi3bp	UTSW	16	56,438,575 (GRCm39)	splice site	probably null
R9464:Abi3bp	UTSW	16	56,409,046 (GRCm39)	missense	possibly damaging	0.48
R9506:Abi3bp	UTSW	16	56,437,773 (GRCm39)	missense	unknown
RF008:Abi3bp	UTSW	16	56,447,952 (GRCm39)	intron	probably benign
RF016:Abi3bp	UTSW	16	56,447,950 (GRCm39)	frame shift	probably null
RF052:Abi3bp	UTSW	16	56,447,948 (GRCm39)	intron	probably benign
RF061:Abi3bp	UTSW	16	56,447,950 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- TACTTCTGCATAGTCTTGCTGG -3'
(R):5'- CTGCTTAGAGAGATGCATGCTTG -3'

Sequencing Primer
(F):5'- CTGTCCGGATGGGTACACATG -3'
(R):5'- ATGCTTGCACACTAGCGG -3'

Posted On

2022-01-20