Mutagenetix > Incidental Mutation

Incidental Mutation 'R9142:Zfp932'

694382

Institutional Source

Beutler Lab

Gene Symbol

Zfp932

Ensembl Gene

ENSMUSG00000066613

Gene Name

zinc finger protein 932

Synonyms

2310001H12Rik

MMRRC Submission

068934-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R9142 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

110144387-110158277 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 110157520 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 406 (H406L)

Ref Sequence

ENSEMBL: ENSMUSP00000108159 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099484] [ENSMUST00000112536] [ENSMUST00000112540] [ENSMUST00000125213] [ENSMUST00000143639] [ENSMUST00000187241]

AlphaFold

E9QAG8

Predicted Effect

probably benign
Transcript: ENSMUST00000099484

SMART Domains

Protein: ENSMUSP00000097083
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000112536
AA Change: H373L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108155
Gene: ENSMUSG00000066613
AA Change: H373L

Domain	Start	End	E-Value	Type
Blast:KRAB	1	31	5e-12	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
ZnF_C2H2	183	205	1.3e-4	SMART
ZnF_C2H2	211	233	1.1e-2	SMART
ZnF_C2H2	239	261	2.27e-4	SMART
ZnF_C2H2	267	289	1.84e-4	SMART
ZnF_C2H2	295	317	5.21e-4	SMART
ZnF_C2H2	323	345	9.73e-4	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	1.58e-3	SMART
ZnF_C2H2	407	429	5.14e-3	SMART
ZnF_C2H2	435	457	2.61e-4	SMART
ZnF_C2H2	463	485	2.43e-4	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000112540
AA Change: H406L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000108159
Gene: ENSMUSG00000066613
AA Change: H406L

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART
ZnF_C2H2	104	126	1.1e-2	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	3.39e-3	SMART
ZnF_C2H2	188	210	1.76e-1	SMART
ZnF_C2H2	216	238	1.3e-4	SMART
ZnF_C2H2	244	266	1.1e-2	SMART
ZnF_C2H2	272	294	2.27e-4	SMART
ZnF_C2H2	300	322	1.84e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	9.73e-4	SMART
ZnF_C2H2	384	406	1.58e-3	SMART
ZnF_C2H2	412	434	1.58e-3	SMART
ZnF_C2H2	440	462	5.14e-3	SMART
ZnF_C2H2	468	490	2.61e-4	SMART
ZnF_C2H2	496	518	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125213

SMART Domains

Protein: ENSMUSP00000119843
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	2e-14	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
Pfam:zf-C2H2_6	182	191	7.3e-1	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000143639

SMART Domains

Protein: ENSMUSP00000121749
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	4	60	1.74e-14	SMART
ZnF_C2H2	103	125	1.1e-2	SMART
ZnF_C2H2	131	153	5.21e-4	SMART
ZnF_C2H2	159	181	3.39e-3	SMART
ZnF_C2H2	187	209	1.76e-1	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000187241
AA Change: H406L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000140446
Gene: ENSMUSG00000066613
AA Change: H406L

Domain	Start	End	E-Value	Type
KRAB	5	61	7.1e-17	SMART
ZnF_C2H2	104	126	4.8e-5	SMART
ZnF_C2H2	132	154	2.2e-6	SMART
ZnF_C2H2	160	182	1.5e-5	SMART
ZnF_C2H2	188	210	7.2e-4	SMART
ZnF_C2H2	216	238	5.4e-7	SMART
ZnF_C2H2	244	266	4.5e-5	SMART
ZnF_C2H2	272	294	9.2e-7	SMART
ZnF_C2H2	300	322	7.5e-7	SMART
ZnF_C2H2	328	350	2.1e-6	SMART
ZnF_C2H2	356	378	4e-6	SMART
ZnF_C2H2	384	406	6.5e-6	SMART
ZnF_C2H2	412	434	6.9e-6	SMART
ZnF_C2H2	440	462	2.1e-5	SMART
ZnF_C2H2	468	490	1.1e-6	SMART
ZnF_C2H2	496	518	1.1e-6	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (64/64)

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adprs	C	A	4: 126,215,360 (GRCm39)	G35V	probably damaging	Het
Apob	A	C	12: 8,062,705 (GRCm39)	D3729A	possibly damaging	Het
App	G	A	16: 84,900,127 (GRCm39)	H108Y	probably damaging	Het
Arap3	G	A	18: 38,112,934 (GRCm39)	A1092V	possibly damaging	Het
Arid3a	T	C	10: 79,787,612 (GRCm39)	S550P	unknown	Het
Armc2	T	G	10: 41,851,404 (GRCm39)	E259D	probably benign	Het
Atp13a2	A	G	4: 140,729,364 (GRCm39)	D566G	probably damaging	Het
Bnc2	A	G	4: 84,474,111 (GRCm39)	C12R	probably benign	Het
Btbd16	C	T	7: 130,417,516 (GRCm39)	R344C	probably damaging	Het
Cc2d2b	C	T	19: 40,753,845 (GRCm39)	R123*	probably null	Het
Cdc37l1	T	A	19: 28,989,402 (GRCm39)	H286Q	possibly damaging	Het
Cfap54	A	T	10: 92,820,097 (GRCm39)	L1239Q	possibly damaging	Het
Cfap58	T	C	19: 47,974,993 (GRCm39)		probably null	Het
Col6a3	A	T	1: 90,706,566 (GRCm39)	D2789E	unknown	Het
Ctnna2	G	T	6: 76,879,423 (GRCm39)		probably benign	Het
Dazl	A	G	17: 50,590,178 (GRCm39)	V271A	probably benign	Het
Dennd4c	A	T	4: 86,755,637 (GRCm39)	N1610Y	probably benign	Het
Dusp13b	T	A	14: 21,792,756 (GRCm39)	K56N	probably benign	Het
Erbb2	T	A	11: 98,312,884 (GRCm39)	I149N	probably damaging	Het
Erbb4	A	T	1: 68,388,552 (GRCm39)	C217S	probably damaging	Het
Fbxw21	A	G	9: 108,985,413 (GRCm39)	C122R	probably damaging	Het
Fktn	G	A	4: 53,734,854 (GRCm39)	G125D	probably benign	Het
Foxm1	T	A	6: 128,344,298 (GRCm39)	I182N	probably damaging	Het
Ggnbp2	T	C	11: 84,730,886 (GRCm39)	N381S	possibly damaging	Het
Gja3	T	C	14: 57,274,048 (GRCm39)	E108G	probably benign	Het
Gm40460	C	A	7: 141,794,499 (GRCm39)	G106V	unknown	Het
Gm9736	A	G	10: 77,586,849 (GRCm39)	C114R	unknown	Het
Gpbp1	A	T	13: 111,563,033 (GRCm39)	D492E	unknown	Het
Gper1	T	C	5: 139,412,312 (GRCm39)	V219A	possibly damaging	Het
Gtf2e1	C	T	16: 37,356,364 (GRCm39)	R56Q	probably benign	Het
H2ac10	T	C	13: 23,718,151 (GRCm39)	L24P	probably damaging	Het
Ide	T	C	19: 37,307,898 (GRCm39)	N38S		Het
Kat6a	T	A	8: 23,430,072 (GRCm39)	M1809K	unknown	Het
Kdm2b	A	T	5: 123,127,112 (GRCm39)		probably benign	Het
Kif7	A	T	7: 79,356,585 (GRCm39)	M702K	probably benign	Het
Lrrtm4	T	A	6: 79,999,426 (GRCm39)	N279K	probably damaging	Het
Mcrip1	G	A	11: 120,435,542 (GRCm39)	P31L	probably damaging	Het
Mettl25b	A	G	3: 87,831,195 (GRCm39)	I444T	probably benign	Het
Nf1	T	A	11: 79,362,315 (GRCm39)	S1497R	probably damaging	Het
Nf1	T	A	11: 79,366,688 (GRCm39)	I1607N	probably damaging	Het
Nrde2	G	T	12: 100,117,518 (GRCm39)	T47K	probably benign	Het
Or5w12	C	T	2: 87,502,313 (GRCm39)	V133I	probably benign	Het
Or6b2b	T	G	1: 92,419,411 (GRCm39)	E22A	probably benign	Het
Or6c1	T	C	10: 129,518,285 (GRCm39)	T108A	probably benign	Het
Ppargc1a	G	A	5: 51,652,146 (GRCm39)	T184I	possibly damaging	Het
Ppp1r15a	T	C	7: 45,173,920 (GRCm39)	D296G	probably damaging	Het
Prl7c1	C	T	13: 27,964,751 (GRCm39)		probably benign	Het
Prss42	G	T	9: 110,628,228 (GRCm39)	R124L	possibly damaging	Het
Psmd1	T	G	1: 86,064,817 (GRCm39)	Y950D	probably damaging	Het
Ptprr	A	T	10: 116,024,119 (GRCm39)	L250F	possibly damaging	Het
Rabgef1	T	A	5: 130,237,554 (GRCm39)	M208K	possibly damaging	Het
Rps6ka2	A	G	17: 7,437,793 (GRCm39)	Y17C	probably damaging	Het
Scart2	T	A	7: 139,877,806 (GRCm39)	D929E	probably damaging	Het
Sec23ip	T	A	7: 128,363,226 (GRCm39)	C463S	probably damaging	Het
Serpina3n	A	G	12: 104,379,710 (GRCm39)	T368A	probably damaging	Het
Sez6l	C	T	5: 112,609,083 (GRCm39)	A589T	probably benign	Het
Smok2b	G	A	17: 13,453,637 (GRCm39)		probably null	Het
Spag5	T	A	11: 78,192,823 (GRCm39)	Y52N	possibly damaging	Het
Tenm3	G	A	8: 48,788,548 (GRCm39)	S494L	unknown	Het
Top3b	T	C	16: 16,701,299 (GRCm39)	Y204H	probably damaging	Het
Trim43b	C	T	9: 88,973,452 (GRCm39)	V94M	possibly damaging	Het
Trps1	T	G	15: 50,524,658 (GRCm39)	I1091L	probably damaging	Het
Ttk	G	A	9: 83,725,741 (GRCm39)	R221Q	probably damaging	Het
Ttn	A	C	2: 76,549,466 (GRCm39)	M31737R	probably damaging	Het
Uso1	T	A	5: 92,335,125 (GRCm39)	L495*	probably null	Het
Usp19	A	G	9: 108,372,284 (GRCm39)	D447G	possibly damaging	Het
Zdhhc2	A	G	8: 40,920,563 (GRCm39)	E274G	probably damaging	Het
Zfp106	T	A	2: 120,350,935 (GRCm39)	Y1595F	probably damaging	Het

Other mutations in Zfp932

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0153:Zfp932	UTSW	5	110,154,834 (GRCm39)	missense	probably benign	0.27
R0268:Zfp932	UTSW	5	110,156,929 (GRCm39)	missense	probably benign	0.24
R1673:Zfp932	UTSW	5	110,156,854 (GRCm39)	missense	probably damaging	1.00
R1797:Zfp932	UTSW	5	110,144,489 (GRCm39)	start gained	probably benign
R1893:Zfp932	UTSW	5	110,157,069 (GRCm39)	missense	possibly damaging	0.71
R1942:Zfp932	UTSW	5	110,154,853 (GRCm39)	missense	probably damaging	1.00
R2073:Zfp932	UTSW	5	110,157,684 (GRCm39)	missense	possibly damaging	0.60
R2076:Zfp932	UTSW	5	110,157,334 (GRCm39)	missense	probably benign	0.01
R2329:Zfp932	UTSW	5	110,157,406 (GRCm39)	missense	probably benign	0.01
R3944:Zfp932	UTSW	5	110,157,820 (GRCm39)	missense	probably benign	0.37
R4551:Zfp932	UTSW	5	110,157,505 (GRCm39)	missense	probably benign	0.03
R4679:Zfp932	UTSW	5	110,157,760 (GRCm39)	missense	probably damaging	1.00
R4692:Zfp932	UTSW	5	110,157,052 (GRCm39)	missense	probably damaging	1.00
R5116:Zfp932	UTSW	5	110,157,242 (GRCm39)	missense	probably benign	0.05
R5260:Zfp932	UTSW	5	110,157,501 (GRCm39)	nonsense	probably null
R5536:Zfp932	UTSW	5	110,157,713 (GRCm39)	nonsense	probably null
R6351:Zfp932	UTSW	5	110,157,209 (GRCm39)	nonsense	probably null
R6786:Zfp932	UTSW	5	110,157,606 (GRCm39)	missense	probably damaging	0.98
R6843:Zfp932	UTSW	5	110,156,581 (GRCm39)	missense	probably benign	0.02
R7437:Zfp932	UTSW	5	110,157,880 (GRCm39)	missense	probably benign	0.10
R7496:Zfp932	UTSW	5	110,156,694 (GRCm39)	missense	probably damaging	0.97
R7704:Zfp932	UTSW	5	110,157,630 (GRCm39)	missense	probably benign	0.09
R8224:Zfp932	UTSW	5	110,144,480 (GRCm39)	start gained	probably benign
R8317:Zfp932	UTSW	5	110,156,922 (GRCm39)	nonsense	probably null
R8765:Zfp932	UTSW	5	110,154,827 (GRCm39)	missense	probably benign	0.01
R8967:Zfp932	UTSW	5	110,156,883 (GRCm39)	missense	probably benign	0.00
R9083:Zfp932	UTSW	5	110,157,100 (GRCm39)	missense	probably damaging	1.00
R9511:Zfp932	UTSW	5	110,155,177 (GRCm39)	missense	possibly damaging	0.46
R9533:Zfp932	UTSW	5	110,157,787 (GRCm39)	missense	probably damaging	1.00
R9640:Zfp932	UTSW	5	110,157,064 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CTTACAAATGTGATCAGTGTGGT -3'
(R):5'- CACATTTGTAGGGTTTCTCTCCAG -3'

Sequencing Primer
(F):5'- ATCAGTGTGGTAAAGCCTTTGCC -3'
(R):5'- GCCACATTGCTCACATTCATAGGG -3'

Posted On

2022-01-20