Mutagenetix > Incidental Mutation

Incidental Mutation 'R9145:Olfr401'

694587

Institutional Source

Beutler Lab

Gene Symbol

Olfr401

Ensembl Gene

ENSMUSG00000070380

Gene Name

olfactory receptor 401

Synonyms

MOR255-6, GA_x6K02T2P1NL-4278037-4278984

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R9145 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

74113712-74122941 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 74121700 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 137 (T137I)

Ref Sequence

ENSEMBL: ENSMUSP00000149542 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079827] [ENSMUST00000213754]

AlphaFold

Q8VFX6

Predicted Effect

probably benign
Transcript: ENSMUST00000079827
AA Change: T137I

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000078756
Gene: ENSMUSG00000070380
AA Change: T137I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	34	311	9.4e-51	PFAM
Pfam:7tm_1	44	293	2.2e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213754
AA Change: T137I

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Meta Mutation Damage Score

0.1624

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (79/79)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	T	A	6: 128,559,069	H720L	probably benign	Het
Abca15	T	C	7: 120,388,165	Y1225H	probably benign	Het
Adck1	C	A	12: 88,368,423	N26K	probably benign	Het
Adcy3	A	G	12: 4,195,208	D382G	probably damaging	Het
Ahi1	T	A	10: 21,000,589	C800S	probably benign	Het
Ahnak	G	T	19: 9,014,923	V4524L	probably benign	Het
Apol11a	C	T	15: 77,513,578	A43V	probably benign	Het
Arid1a	T	C	4: 133,693,903	M479V	unknown	Het
Atp13a2	T	A	4: 140,996,745	C324S	probably damaging	Het
C2cd2	T	G	16: 97,876,286	T413P	probably damaging	Het
Cct5	A	C	15: 31,590,961	D531E		Het
Chst1	A	T	2: 92,614,178	I332F	probably damaging	Het
Col12a1	A	T	9: 79,620,062	V2662D	probably benign	Het
Cts3	A	G	13: 61,564,986	Y307H	probably benign	Het
D630023F18Rik	G	A	1: 65,121,212		probably benign	Het
Dhx9	A	G	1: 153,461,080	I807T	probably damaging	Het
Doxl2	C	A	6: 48,975,956	R272S	probably benign	Het
Exoc3l4	T	C	12: 111,422,152	L25P	probably benign	Het
Ggcx	T	A	6: 72,425,922	C288S	probably benign	Het
Gjb3	A	G	4: 127,326,347	Y131H	probably damaging	Het
Hdhd3	A	G	4: 62,499,337	S201P	probably benign	Het
Helz2	A	T	2: 181,240,055	V315E	probably damaging	Het
Hsp90aa1	A	G	12: 110,696,250		probably null	Het
Il12a	C	T	3: 68,691,542	R19W	unknown	Het
Isoc1	C	T	18: 58,673,275	A219V	possibly damaging	Het
Jup	T	A	11: 100,378,298	T430S	probably benign	Het
Klra5	C	T	6: 129,909,948	C39Y	probably benign	Het
Map4	A	T	9: 110,026,200	Q131L	probably damaging	Het
Mau2	T	A	8: 70,027,515	K314M	probably damaging	Het
Mga	A	G	2: 119,964,012	M2726V	probably benign	Het
Mgme1	G	A	2: 144,272,485		probably null	Het
Msrb2	A	G	2: 19,394,255	E143G	probably benign	Het
Muc5b	G	T	7: 141,857,613	C1432F	unknown	Het
Naglu	T	C	11: 101,071,114	Y138H	probably damaging	Het
Nicn1	C	T	9: 108,294,509	R163C	possibly damaging	Het
Nlrp1b	A	G	11: 71,218,367	Y103H	probably benign	Het
Nodal	A	G	10: 61,423,680	N299D	probably damaging	Het
Notch1	T	C	2: 26,459,575	T2518A	probably benign	Het
Nr2e1	A	C	10: 42,572,952	S97A	probably benign	Het
Nuak1	A	T	10: 84,374,723	S500R	probably benign	Het
Nxt2	C	T	X: 142,237,751	A118V	possibly damaging	Het
Nyap1	T	C	5: 137,737,913	E104G	probably benign	Het
Olfr670	T	A	7: 104,959,997	H245L	probably damaging	Het
Palld	T	C	8: 61,877,073	T257A	probably benign	Het
Pcnx4	C	T	12: 72,556,269	P435L	probably damaging	Het
Piezo1	T	C	8: 122,482,014	T2538A	unknown	Het
Plagl1	T	C	10: 13,128,128	L380P	unknown	Het
Polrmt	G	T	10: 79,740,581	Q514K	probably benign	Het
Psg17	T	G	7: 18,819,926	D133A	probably benign	Het
Rab3b	G	A	4: 108,940,706	D185N	probably benign	Het
Ranbp2	T	A	10: 58,455,914	S248T	probably benign	Het
Rdh10	C	A	1: 16,129,206	A212D	probably damaging	Het
Rinl	A	G	7: 28,795,664	H154R		Het
Selenbp1	T	C	3: 94,944,103	M389T	probably benign	Het
Serpina1c	T	A	12: 103,896,141	H305L	possibly damaging	Het
Sh3rf2	T	A	18: 42,149,681	S467T		Het
Sipa1l1	A	G	12: 82,396,561	D875G	probably benign	Het
Slc10a6	A	T	5: 103,628,934	V100D	probably damaging	Het
Slc13a4	T	C	6: 35,270,355	I577V	possibly damaging	Het
Slc20a2	T	C	8: 22,540,431	F168L	probably benign	Het
Slc25a1	A	T	16: 17,927,244		probably null	Het
Slc5a12	A	G	2: 110,640,897	S495G	probably benign	Het
Smpdl3a	A	G	10: 57,800,932	D42G	possibly damaging	Het
Spag16	G	C	1: 70,381,300	L482F	probably damaging	Het
Susd5	G	A	9: 114,096,221	G391R	probably damaging	Het
Sync	G	T	4: 129,293,825	A217S		Het
Tha1	T	A	11: 117,868,686	N326Y	probably damaging	Het
Tjp1	A	T	7: 65,302,816	F1590Y	probably benign	Het
Tle4	T	C	19: 14,468,219	N221S	probably benign	Het
Tln1	T	C	4: 43,536,024	T2054A	probably damaging	Het
Ttc30a1	A	T	2: 75,980,079	Y553*	probably null	Het
Ttll4	A	G	1: 74,679,790	K267E	probably benign	Het
Usp32	C	T	11: 85,022,292	G930D	probably damaging	Het
Vmn2r75	T	C	7: 86,164,239	N452D	probably damaging	Het
Vmn2r81	T	C	10: 79,268,194	L217P	possibly damaging	Het
Wnt2	T	A	6: 18,030,398		probably benign	Het
Zfp459	C	T	13: 67,408,616	S116N	probably benign	Het
Zfp981	C	A	4: 146,537,953	T445N	possibly damaging	Het

Other mutations in Olfr401

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Olfr401	APN	11	74121879	missense	probably benign	0.00
IGL01303:Olfr401	APN	11	74121334	missense	probably damaging	1.00
IGL01397:Olfr401	APN	11	74121764	missense	probably damaging	0.97
IGL02101:Olfr401	APN	11	74121745	nonsense	probably null
IGL02121:Olfr401	APN	11	74121287	splice site	probably benign
IGL02347:Olfr401	APN	11	74121571	missense	probably benign	0.03
IGL03113:Olfr401	APN	11	74121703	missense	probably benign	0.13
IGL03126:Olfr401	APN	11	74121784	missense	probably benign	0.01
IGL03302:Olfr401	APN	11	74121633	missense	possibly damaging	0.60
R0549:Olfr401	UTSW	11	74121475	missense	probably damaging	1.00
R1180:Olfr401	UTSW	11	74121580	missense	probably benign	0.28
R1350:Olfr401	UTSW	11	74122213	missense	possibly damaging	0.96
R1800:Olfr401	UTSW	11	74121408	missense	probably benign	0.00
R1808:Olfr401	UTSW	11	74121431	missense	probably damaging	0.96
R1962:Olfr401	UTSW	11	74121824	missense	probably benign	0.02
R1998:Olfr401	UTSW	11	74121580	missense	probably benign	0.28
R1999:Olfr401	UTSW	11	74121580	missense	probably benign	0.28
R2000:Olfr401	UTSW	11	74121580	missense	probably benign	0.28
R2859:Olfr401	UTSW	11	74121982	missense	probably damaging	1.00
R4914:Olfr401	UTSW	11	74121879	missense	probably benign	0.00
R4915:Olfr401	UTSW	11	74121879	missense	probably benign	0.00
R4916:Olfr401	UTSW	11	74121879	missense	probably benign	0.00
R4918:Olfr401	UTSW	11	74121879	missense	probably benign	0.00
R5292:Olfr401	UTSW	11	74122051	missense	probably damaging	0.99
R5522:Olfr401	UTSW	11	74121658	missense	probably damaging	1.00
R5761:Olfr401	UTSW	11	74121509	missense	probably damaging	0.97
R6318:Olfr401	UTSW	11	74121721	missense	possibly damaging	0.82
R6608:Olfr401	UTSW	11	74121628	missense	probably benign	0.00
R6737:Olfr401	UTSW	11	74121906	missense	probably benign
R6790:Olfr401	UTSW	11	74121601	missense	probably damaging	1.00
R7243:Olfr401	UTSW	11	74121733	missense	probably damaging	0.97
R7423:Olfr401	UTSW	11	74121985	missense	probably benign	0.01
R7517:Olfr401	UTSW	11	74121509	missense	probably damaging	0.97
R7833:Olfr401	UTSW	11	74121837	missense	probably damaging	1.00
R8241:Olfr401	UTSW	11	74122209	missense	probably benign	0.03
R8423:Olfr401	UTSW	11	74121666	missense	probably benign	0.06
R8937:Olfr401	UTSW	11	74122048	missense	probably benign	0.03
R9725:Olfr401	UTSW	11	74121786	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTCCTGGGGAATCTGTCAG -3'
(R):5'- TGATCTGGGTACTGGAGCAG -3'

Sequencing Primer
(F):5'- GGGTGCATCAGTGTCACTATTCC -3'
(R):5'- TCTGGGTACTGGAGCAGGAGAG -3'

Posted On

2022-01-20