Mutagenetix > Incidental Mutation

Incidental Mutation 'R9148:Wnk1'

694795

Institutional Source

Beutler Lab

Gene Symbol

Wnk1

Ensembl Gene

ENSMUSG00000045962

Gene Name

WNK lysine deficient protein kinase 1

Synonyms

Prkwnk1, 6430573H23Rik, EG406236, Hsn2

MMRRC Submission

068937-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R9148 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

119900930-120015633 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 119925631 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 1527 (S1527P)

Ref Sequence

ENSEMBL: ENSMUSP00000063001 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060043] [ENSMUST00000088644] [ENSMUST00000088646] [ENSMUST00000177761] [ENSMUST00000203030]

AlphaFold

P83741

Predicted Effect

unknown
Transcript: ENSMUST00000060043
AA Change: S1527P

SMART Domains

Protein: ENSMUSP00000063001
Gene: ENSMUSG00000045962
AA Change: S1527P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.5e-44	PFAM
Pfam:Pkinase	221	479	4.4e-58	PFAM
Pfam:OSR1_C	500	537	2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	865	876	N/A	INTRINSIC
low complexity region	1018	1028	N/A	INTRINSIC
low complexity region	1042	1058	N/A	INTRINSIC
internal_repeat_1	1136	1178	2.15e-5	PROSPERO
low complexity region	1289	1305	N/A	INTRINSIC
low complexity region	1345	1357	N/A	INTRINSIC
low complexity region	1379	1396	N/A	INTRINSIC
low complexity region	1398	1428	N/A	INTRINSIC
low complexity region	1429	1454	N/A	INTRINSIC
low complexity region	1496	1515	N/A	INTRINSIC
low complexity region	1542	1556	N/A	INTRINSIC
low complexity region	1586	1609	N/A	INTRINSIC
low complexity region	1670	1691	N/A	INTRINSIC
low complexity region	1698	1712	N/A	INTRINSIC
low complexity region	1738	1764	N/A	INTRINSIC
low complexity region	1882	1900	N/A	INTRINSIC
coiled coil region	2065	2092	N/A	INTRINSIC
low complexity region	2103	2114	N/A	INTRINSIC
low complexity region	2116	2140	N/A	INTRINSIC
low complexity region	2208	2232	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000088644
AA Change: S1785P

SMART Domains

Protein: ENSMUSP00000086017
Gene: ENSMUSG00000045962
AA Change: S1785P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	5.5e-44	PFAM
Pfam:Pkinase	221	479	4.3e-56	PFAM
Pfam:OSR1_C	500	537	1.9e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	742	753	N/A	INTRINSIC
low complexity region	919	935	N/A	INTRINSIC
low complexity region	1050	1063	N/A	INTRINSIC
low complexity region	1067	1076	N/A	INTRINSIC
low complexity region	1146	1158	N/A	INTRINSIC
low complexity region	1276	1286	N/A	INTRINSIC
low complexity region	1300	1316	N/A	INTRINSIC
internal_repeat_1	1394	1436	2.19e-5	PROSPERO
low complexity region	1547	1563	N/A	INTRINSIC
low complexity region	1603	1615	N/A	INTRINSIC
low complexity region	1637	1654	N/A	INTRINSIC
low complexity region	1656	1686	N/A	INTRINSIC
low complexity region	1687	1712	N/A	INTRINSIC
low complexity region	1754	1773	N/A	INTRINSIC
low complexity region	1800	1814	N/A	INTRINSIC
low complexity region	1844	1867	N/A	INTRINSIC
low complexity region	1928	1949	N/A	INTRINSIC
low complexity region	1956	1970	N/A	INTRINSIC
low complexity region	1996	2022	N/A	INTRINSIC
low complexity region	2140	2158	N/A	INTRINSIC
coiled coil region	2323	2350	N/A	INTRINSIC
low complexity region	2361	2372	N/A	INTRINSIC
low complexity region	2374	2398	N/A	INTRINSIC
low complexity region	2466	2490	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000088646
AA Change: S1373P

SMART Domains

Protein: ENSMUSP00000086019
Gene: ENSMUSG00000045962
AA Change: S1373P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.3e-44	PFAM
Pfam:Pkinase	221	479	4e-58	PFAM
Pfam:OSR1_C	500	537	1.8e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	864	874	N/A	INTRINSIC
low complexity region	888	904	N/A	INTRINSIC
internal_repeat_1	982	1024	7.56e-6	PROSPERO
low complexity region	1135	1151	N/A	INTRINSIC
low complexity region	1191	1203	N/A	INTRINSIC
low complexity region	1225	1242	N/A	INTRINSIC
low complexity region	1244	1274	N/A	INTRINSIC
low complexity region	1275	1300	N/A	INTRINSIC
low complexity region	1342	1361	N/A	INTRINSIC
low complexity region	1388	1402	N/A	INTRINSIC
low complexity region	1432	1455	N/A	INTRINSIC
low complexity region	1516	1537	N/A	INTRINSIC
low complexity region	1544	1558	N/A	INTRINSIC
low complexity region	1584	1610	N/A	INTRINSIC
low complexity region	1700	1718	N/A	INTRINSIC
coiled coil region	1883	1910	N/A	INTRINSIC
low complexity region	1921	1932	N/A	INTRINSIC
low complexity region	1934	1958	N/A	INTRINSIC
low complexity region	2026	2050	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000159785

SMART Domains

Protein: ENSMUSP00000124905
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	159	169	N/A	INTRINSIC
low complexity region	183	199	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161048

SMART Domains

Protein: ENSMUSP00000125345
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
Blast:S_TKc	2	68	3e-34	BLAST
SCOP:d1phk__	3	70	2e-7	SMART
Pfam:OSR1_C	89	126	9.5e-21	PFAM
coiled coil region	151	185	N/A	INTRINSIC
low complexity region	323	335	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161802

SMART Domains

Protein: ENSMUSP00000124724
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	24	50	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162368

SMART Domains

Protein: ENSMUSP00000125383
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	83	95	N/A	INTRINSIC
low complexity region	142	158	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000162541

SMART Domains

Protein: ENSMUSP00000125193
Gene: ENSMUSG00000045962

Domain	Start	End	E-Value	Type
low complexity region	63	84	N/A	INTRINSIC
low complexity region	91	105	N/A	INTRINSIC
low complexity region	131	157	N/A	INTRINSIC
low complexity region	247	265	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000177761
AA Change: S1776P

PolyPhen 2 Score 0.038 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000136777
Gene: ENSMUSG00000045962
AA Change: S1776P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	1.7e-44	PFAM
Pfam:Pkinase	221	479	5.1e-58	PFAM
Pfam:OSR1_C	500	537	2.2e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	710	722	N/A	INTRINSIC
low complexity region	828	839	N/A	INTRINSIC
low complexity region	1005	1021	N/A	INTRINSIC
low complexity region	1136	1149	N/A	INTRINSIC
low complexity region	1153	1162	N/A	INTRINSIC
low complexity region	1232	1244	N/A	INTRINSIC
low complexity region	1291	1307	N/A	INTRINSIC
internal_repeat_1	1385	1427	1.91e-5	PROSPERO
low complexity region	1538	1554	N/A	INTRINSIC
low complexity region	1594	1606	N/A	INTRINSIC
low complexity region	1628	1645	N/A	INTRINSIC
low complexity region	1647	1677	N/A	INTRINSIC
low complexity region	1678	1703	N/A	INTRINSIC
low complexity region	1745	1764	N/A	INTRINSIC
low complexity region	1791	1805	N/A	INTRINSIC
low complexity region	1835	1858	N/A	INTRINSIC
low complexity region	1919	1940	N/A	INTRINSIC
low complexity region	1947	1961	N/A	INTRINSIC
low complexity region	1987	2013	N/A	INTRINSIC
low complexity region	2131	2149	N/A	INTRINSIC
coiled coil region	2314	2341	N/A	INTRINSIC
low complexity region	2352	2363	N/A	INTRINSIC
low complexity region	2365	2389	N/A	INTRINSIC
low complexity region	2457	2481	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000203030
AA Change: S1278P

PolyPhen 2 Score 0.038 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000145304
Gene: ENSMUSG00000045962
AA Change: S1278P

Domain	Start	End	E-Value	Type
low complexity region	101	115	N/A	INTRINSIC
low complexity region	127	141	N/A	INTRINSIC
low complexity region	171	193	N/A	INTRINSIC
Pfam:Pkinase_Tyr	221	477	4.1e-44	PFAM
Pfam:Pkinase	221	479	3.2e-56	PFAM
Pfam:OSR1_C	500	537	1.5e-22	PFAM
coiled coil region	563	597	N/A	INTRINSIC
low complexity region	734	746	N/A	INTRINSIC
low complexity region	793	809	N/A	INTRINSIC
internal_repeat_1	887	929	5.8e-6	PROSPERO
low complexity region	1040	1056	N/A	INTRINSIC
low complexity region	1096	1108	N/A	INTRINSIC
low complexity region	1130	1147	N/A	INTRINSIC
low complexity region	1149	1179	N/A	INTRINSIC
low complexity region	1180	1205	N/A	INTRINSIC
low complexity region	1247	1266	N/A	INTRINSIC
low complexity region	1293	1307	N/A	INTRINSIC
low complexity region	1337	1360	N/A	INTRINSIC
low complexity region	1421	1442	N/A	INTRINSIC
low complexity region	1449	1463	N/A	INTRINSIC
low complexity region	1489	1515	N/A	INTRINSIC
low complexity region	1633	1651	N/A	INTRINSIC
coiled coil region	1816	1843	N/A	INTRINSIC
low complexity region	1854	1865	N/A	INTRINSIC
low complexity region	1867	1891	N/A	INTRINSIC
low complexity region	1959	1983	N/A	INTRINSIC

Meta Mutation Damage Score

0.0584

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

96% (94/98)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]
PHENOTYPE: Homozygous mutant mice die before birth, whereas heterozygotes survive and exhibit decreased blood pressure. Mice homozygous for an allele that does not produce the kidney isoform exhibit a slight increase in systemic arterial diastolic blood pressure and reduced sensitivity to amiloride. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 96 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110032F04Rik	T	A	3: 68,777,345 (GRCm39)	I102N	probably damaging	Het
2610008E11Rik	A	T	10: 78,903,406 (GRCm39)	C303*	probably null	Het
A430033K04Rik	T	A	5: 138,644,547 (GRCm39)	V144D	possibly damaging	Het
Adamts13	T	A	2: 26,883,024 (GRCm39)	M858K	probably benign	Het
Ankrd29	T	C	18: 12,408,760 (GRCm39)		probably benign	Het
Arg1	A	C	10: 24,796,655 (GRCm39)	D57E	probably benign	Het
Bend4	G	A	5: 67,557,415 (GRCm39)	T467M	probably damaging	Het
C1s1	T	C	6: 124,517,758 (GRCm39)	D74G	probably damaging	Het
Cacnb2	G	A	2: 14,972,773 (GRCm39)	G227S	possibly damaging	Het
Cbs	T	C	17: 31,844,889 (GRCm39)	D137G	probably damaging	Het
Ccdc136	A	G	6: 29,418,070 (GRCm39)	E846G	probably damaging	Het
Cd6	A	T	19: 10,776,855 (GRCm39)	S57T	probably benign	Het
Cep20	G	A	16: 14,135,222 (GRCm39)		probably benign	Het
Col11a2	T	A	17: 34,273,119 (GRCm39)		probably benign	Het
Col7a1	T	C	9: 108,789,274 (GRCm39)	S829P	unknown	Het
Ctsj	T	C	13: 61,149,249 (GRCm39)	Y264C	probably damaging	Het
Dennd3	T	A	15: 73,429,463 (GRCm39)	N915K	probably damaging	Het
E2f2	A	T	4: 135,908,595 (GRCm39)		probably null	Het
Eme1	T	C	11: 94,538,855 (GRCm39)	T342A	possibly damaging	Het
Epc1	A	T	18: 6,453,266 (GRCm39)		probably benign	Het
Fat1	T	C	8: 45,405,682 (GRCm39)	V811A	possibly damaging	Het
Fbxo2	A	G	4: 148,250,166 (GRCm39)	E232G	probably damaging	Het
Flnb	G	T	14: 7,817,996 (GRCm38)		probably benign	Het
Fmn1	A	C	2: 113,271,973 (GRCm39)	N114H	unknown	Het
Foxp2	A	T	6: 15,286,711 (GRCm39)	Q67L	possibly damaging	Het
Galntl5	A	T	5: 25,415,353 (GRCm39)	I295L	possibly damaging	Het
Gjd4	T	A	18: 9,280,095 (GRCm39)	T328S	probably benign	Het
Hint2	A	G	4: 43,654,952 (GRCm39)	I59T	probably damaging	Het
Hpn	C	T	7: 30,802,043 (GRCm39)	R252Q	probably benign	Het
Hspa12a	T	G	19: 58,793,890 (GRCm39)	T324P	probably damaging	Het
Ighv4-1	A	T	12: 113,912,000 (GRCm39)	D84E	possibly damaging	Het
Il1r2	T	A	1: 40,151,258 (GRCm39)	I171N	probably damaging	Het
Il31ra	C	T	13: 112,670,276 (GRCm39)	E371K	probably benign	Het
Klhl24	C	T	16: 19,936,690 (GRCm39)	R433C	probably damaging	Het
Klra5	C	T	6: 129,886,911 (GRCm39)	C39Y	probably benign	Het
Lypd8l	T	A	11: 58,499,338 (GRCm39)	D160V	probably benign	Het
Macc1	T	C	12: 119,414,091 (GRCm39)	S756P	possibly damaging	Het
Mael	A	G	1: 166,029,259 (GRCm39)	V388A	probably benign	Het
Mast3	A	G	8: 71,233,091 (GRCm39)	S1074P	probably damaging	Het
Med13	A	G	11: 86,192,297 (GRCm39)	C823R	probably benign	Het
Megf6	A	G	4: 154,339,130 (GRCm39)	T556A	probably benign	Het
Mlip	A	G	9: 77,045,694 (GRCm39)	F14L	possibly damaging	Het
Moxd2	C	A	6: 40,860,978 (GRCm39)	C271F	probably damaging	Het
Myo1e	A	T	9: 70,283,830 (GRCm39)	D842V	probably damaging	Het
Ndst4	C	T	3: 125,231,722 (GRCm39)	A97V	probably damaging	Het
Nherf4	A	G	9: 44,160,676 (GRCm39)	C240R	probably damaging	Het
Nicn1	C	T	9: 108,171,708 (GRCm39)	R163C	possibly damaging	Het
Nos1	G	C	5: 118,017,402 (GRCm39)	R255P	probably benign	Het
Nptx2	A	G	5: 144,492,980 (GRCm39)	T356A	probably benign	Het
Nup160	A	G	2: 90,533,489 (GRCm39)	D646G	probably damaging	Het
Nup98	C	A	7: 101,788,037 (GRCm39)	R1011L	probably benign	Het
Nxt2	C	T	X: 141,020,747 (GRCm39)	A118V	possibly damaging	Het
Or1j19	A	G	2: 36,676,938 (GRCm39)	T134A	probably benign	Het
Or1p1	A	C	11: 74,180,169 (GRCm39)	Q232H	probably damaging	Het
Or5c1	T	C	2: 37,222,017 (GRCm39)	L86P	possibly damaging	Het
Or7h8	T	A	9: 20,124,358 (GRCm39)	F238I	probably damaging	Het
Or8k24	A	G	2: 86,216,324 (GRCm39)	V146A	probably benign	Het
Osbp2	A	G	11: 3,665,143 (GRCm39)	L67P	probably damaging	Het
Pabpc6	C	T	17: 9,886,937 (GRCm39)	S538N	probably benign	Het
Pcdhga1	C	A	18: 37,796,433 (GRCm39)	A479D	possibly damaging	Het
Pdgfd	A	T	9: 6,333,328 (GRCm39)	D172V	probably benign	Het
Pfpl	A	T	19: 12,405,804 (GRCm39)	E18D	possibly damaging	Het
Plat	A	G	8: 23,268,466 (GRCm39)	T373A	probably damaging	Het
Plekhg1	G	A	10: 3,907,527 (GRCm39)	D870N		Het
Ppp1r13b	T	C	12: 111,800,268 (GRCm39)	H626R	probably benign	Het
Ppp6r3	A	T	19: 3,543,974 (GRCm39)	V347D	probably damaging	Het
Prr12	G	T	7: 44,697,242 (GRCm39)	P891H	unknown	Het
Psg22	T	A	7: 18,460,682 (GRCm39)	I437K	probably benign	Het
Ptpra	T	A	2: 30,328,255 (GRCm39)	M192K	probably damaging	Het
Ptpra	G	T	2: 30,328,256 (GRCm39)	M192I	probably benign	Het
Ptprj	G	A	2: 90,288,562 (GRCm39)	S801L	probably benign	Het
Relb	T	C	7: 19,350,276 (GRCm39)	Y228C	probably damaging	Het
Rfx3	A	T	19: 27,878,207 (GRCm39)	V30E	possibly damaging	Het
Rgs2	A	G	1: 143,877,925 (GRCm39)	S124P	probably damaging	Het
Rph3a	T	C	5: 121,086,880 (GRCm39)	N440S	possibly damaging	Het
Ryr2	T	C	13: 11,900,424 (GRCm39)	T104A	probably benign	Het
Scn7a	T	C	2: 66,514,507 (GRCm39)	D1089G	possibly damaging	Het
Sebox	A	G	11: 78,395,222 (GRCm39)	D188G	possibly damaging	Het
Serpinf2	A	T	11: 75,323,418 (GRCm39)	F429L	probably damaging	Het
Six4	A	C	12: 73,155,681 (GRCm39)	S429A	probably benign	Het
Slc2a10	A	T	2: 165,357,543 (GRCm39)	H401L	possibly damaging	Het
Spag16	G	C	1: 70,420,459 (GRCm39)	L482F	probably damaging	Het
Ssbp2	T	C	13: 91,842,141 (GRCm39)	S321P	probably damaging	Het
Syne2	A	G	12: 75,937,158 (GRCm39)	E252G	probably damaging	Het
Synj2	T	C	17: 6,084,172 (GRCm39)	I1178T	probably damaging	Het
Tafa5	T	C	15: 87,428,775 (GRCm39)	I28T	probably benign	Het
Tmem132b	C	A	5: 125,864,167 (GRCm39)	Q758K	probably damaging	Het
Tril	A	G	6: 53,795,137 (GRCm39)	L695P	probably damaging	Het
Ubap1l	T	C	9: 65,276,603 (GRCm39)	L35P	probably damaging	Het
Vmn1r225	T	A	17: 20,722,577 (GRCm39)	M6K	possibly damaging	Het
Vmn1r229	T	C	17: 21,034,758 (GRCm39)	M1T	probably null	Het
Vmn2r80	A	T	10: 79,030,687 (GRCm39)	I838F	probably damaging	Het
Vmn2r98	T	A	17: 19,286,383 (GRCm39)	W294R	probably benign	Het
Wnt11	G	A	7: 98,488,241 (GRCm39)		probably benign	Het
Zdhhc17	A	T	10: 110,785,503 (GRCm39)	S436R	possibly damaging	Het
Zfp12	T	C	5: 143,230,389 (GRCm39)	S271P	probably damaging	Het

Other mutations in Wnk1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00901:Wnk1	APN	6	119,937,669 (GRCm39)	missense	probably damaging	1.00
IGL01364:Wnk1	APN	6	119,914,372 (GRCm39)	missense	probably damaging	1.00
IGL01715:Wnk1	APN	6	119,925,358 (GRCm39)	missense	probably damaging	1.00
IGL01951:Wnk1	APN	6	119,940,446 (GRCm39)	missense	probably damaging	0.99
IGL02043:Wnk1	APN	6	119,926,039 (GRCm39)	unclassified	probably benign
IGL02268:Wnk1	APN	6	119,914,334 (GRCm39)	nonsense	probably null
IGL02348:Wnk1	APN	6	119,940,289 (GRCm39)	missense	probably damaging	0.98
IGL02425:Wnk1	APN	6	119,940,415 (GRCm39)	missense	probably damaging	1.00
IGL02850:Wnk1	APN	6	119,914,823 (GRCm39)	missense	probably benign	0.26
IGL03160:Wnk1	APN	6	119,903,594 (GRCm39)	missense	probably damaging	1.00
IGL03387:Wnk1	APN	6	119,931,148 (GRCm39)	missense	possibly damaging	0.76
IGL03405:Wnk1	APN	6	119,930,856 (GRCm39)	missense	probably benign	0.41
Actor	UTSW	6	119,947,939 (GRCm39)	missense	probably damaging	1.00
Bad	UTSW	6	119,942,639 (GRCm39)	intron	probably benign
Blink	UTSW	6	119,979,234 (GRCm39)	nonsense	probably null
Knock	UTSW	6	119,920,839 (GRCm39)	splice site	probably null
Narrow	UTSW	6	119,969,369 (GRCm39)	missense	probably damaging	1.00
nictitate	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
opportunity	UTSW	6	119,928,029 (GRCm39)	missense	probably damaging	1.00
path	UTSW	6	120,014,110 (GRCm39)	missense	probably damaging	0.99
Stormy	UTSW	6	119,969,408 (GRCm39)	missense	probably damaging	1.00
tear	UTSW	6	119,940,431 (GRCm39)	missense	probably damaging	0.98
Tic	UTSW	6	119,927,044 (GRCm39)	missense	probably damaging	1.00
Unforgiving	UTSW	6	119,925,243 (GRCm39)	missense	probably damaging	1.00
Window	UTSW	6	119,969,414 (GRCm39)	missense	probably damaging	1.00
Woke	UTSW	6	119,939,916 (GRCm39)	missense	probably benign	0.12
IGL03052:Wnk1	UTSW	6	119,921,760 (GRCm39)	splice site	probably benign
PIT4480001:Wnk1	UTSW	6	119,940,328 (GRCm39)	nonsense	probably null
R0044:Wnk1	UTSW	6	120,014,110 (GRCm39)	missense	probably damaging	0.99
R0207:Wnk1	UTSW	6	119,929,694 (GRCm39)	missense	probably damaging	1.00
R0333:Wnk1	UTSW	6	119,905,124 (GRCm39)	intron	probably benign
R0453:Wnk1	UTSW	6	119,940,112 (GRCm39)	missense	probably damaging	0.99
R0457:Wnk1	UTSW	6	119,946,293 (GRCm39)	missense	probably damaging	1.00
R0501:Wnk1	UTSW	6	119,939,764 (GRCm39)	missense	probably damaging	1.00
R0525:Wnk1	UTSW	6	119,903,525 (GRCm39)	missense	probably damaging	1.00
R0526:Wnk1	UTSW	6	119,928,953 (GRCm39)	missense	probably damaging	0.99
R0606:Wnk1	UTSW	6	119,903,644 (GRCm39)	missense	probably damaging	1.00
R0658:Wnk1	UTSW	6	119,925,466 (GRCm39)	missense	probably damaging	0.98
R1148:Wnk1	UTSW	6	119,928,967 (GRCm39)	splice site	probably benign
R1188:Wnk1	UTSW	6	119,925,670 (GRCm39)	nonsense	probably null
R1245:Wnk1	UTSW	6	119,925,418 (GRCm39)	missense	probably benign	0.26
R1449:Wnk1	UTSW	6	119,929,779 (GRCm39)	missense	probably damaging	1.00
R1469:Wnk1	UTSW	6	119,927,645 (GRCm39)	splice site	probably benign
R1869:Wnk1	UTSW	6	119,928,050 (GRCm39)	missense	probably damaging	1.00
R1871:Wnk1	UTSW	6	119,928,050 (GRCm39)	missense	probably damaging	1.00
R1928:Wnk1	UTSW	6	119,929,884 (GRCm39)	missense	probably damaging	1.00
R1959:Wnk1	UTSW	6	119,946,208 (GRCm39)	missense	probably damaging	0.98
R1961:Wnk1	UTSW	6	119,946,208 (GRCm39)	missense	probably damaging	0.98
R1964:Wnk1	UTSW	6	119,911,343 (GRCm39)	missense	possibly damaging	0.86
R1983:Wnk1	UTSW	6	119,914,539 (GRCm39)	missense	probably damaging	1.00
R2062:Wnk1	UTSW	6	119,905,118 (GRCm39)	splice site	probably null
R2144:Wnk1	UTSW	6	119,925,949 (GRCm39)	unclassified	probably benign
R2186:Wnk1	UTSW	6	119,925,528 (GRCm39)	missense	probably benign	0.26
R2281:Wnk1	UTSW	6	119,940,601 (GRCm39)	splice site	probably null
R2338:Wnk1	UTSW	6	119,946,495 (GRCm39)	missense	probably benign	0.42
R2420:Wnk1	UTSW	6	119,913,328 (GRCm39)	critical splice donor site	probably null
R3727:Wnk1	UTSW	6	119,969,414 (GRCm39)	missense	probably damaging	1.00
R3773:Wnk1	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
R3836:Wnk1	UTSW	6	119,927,004 (GRCm39)	missense	probably damaging	1.00
R3837:Wnk1	UTSW	6	119,927,004 (GRCm39)	missense	probably damaging	1.00
R3847:Wnk1	UTSW	6	119,946,315 (GRCm39)	missense	possibly damaging	0.70
R3903:Wnk1	UTSW	6	119,926,012 (GRCm39)	missense	probably damaging	1.00
R4031:Wnk1	UTSW	6	119,928,029 (GRCm39)	missense	probably damaging	1.00
R4095:Wnk1	UTSW	6	119,925,087 (GRCm39)	missense	probably damaging	1.00
R4232:Wnk1	UTSW	6	119,926,222 (GRCm39)	missense	possibly damaging	0.90
R4422:Wnk1	UTSW	6	119,930,856 (GRCm39)	missense	probably benign	0.41
R4423:Wnk1	UTSW	6	119,903,387 (GRCm39)	missense	probably damaging	1.00
R4572:Wnk1	UTSW	6	119,928,872 (GRCm39)	missense	possibly damaging	0.49
R4704:Wnk1	UTSW	6	119,942,705 (GRCm39)	missense	possibly damaging	0.83
R4755:Wnk1	UTSW	6	119,940,431 (GRCm39)	missense	probably damaging	0.98
R4812:Wnk1	UTSW	6	119,929,732 (GRCm39)	missense	probably benign	0.16
R4822:Wnk1	UTSW	6	119,939,399 (GRCm39)	missense	probably benign	0.02
R4879:Wnk1	UTSW	6	119,926,338 (GRCm39)	missense	probably damaging	1.00
R4970:Wnk1	UTSW	6	119,942,696 (GRCm39)	intron	probably benign
R5002:Wnk1	UTSW	6	119,914,924 (GRCm39)	missense	probably benign	0.13
R5037:Wnk1	UTSW	6	119,942,696 (GRCm39)	intron	probably benign
R5152:Wnk1	UTSW	6	119,979,241 (GRCm39)	missense	possibly damaging	0.88
R5257:Wnk1	UTSW	6	120,014,149 (GRCm39)	missense	probably benign	0.00
R5354:Wnk1	UTSW	6	119,945,484 (GRCm39)	missense	probably benign	0.01
R5421:Wnk1	UTSW	6	119,929,779 (GRCm39)	missense	probably damaging	1.00
R5564:Wnk1	UTSW	6	119,925,852 (GRCm39)	unclassified	probably benign
R5600:Wnk1	UTSW	6	119,926,319 (GRCm39)	missense	probably damaging	1.00
R5847:Wnk1	UTSW	6	119,969,369 (GRCm39)	missense	probably damaging	1.00
R6083:Wnk1	UTSW	6	120,014,562 (GRCm39)	missense	probably damaging	0.99
R6110:Wnk1	UTSW	6	119,949,958 (GRCm39)	intron	probably benign
R6128:Wnk1	UTSW	6	119,940,747 (GRCm39)	splice site	probably null
R6237:Wnk1	UTSW	6	119,929,728 (GRCm39)	missense	probably damaging	1.00
R6341:Wnk1	UTSW	6	119,925,546 (GRCm39)	missense	probably damaging	1.00
R6467:Wnk1	UTSW	6	119,939,916 (GRCm39)	missense	probably benign	0.12
R6696:Wnk1	UTSW	6	119,925,243 (GRCm39)	missense	probably damaging	1.00
R6888:Wnk1	UTSW	6	119,925,742 (GRCm39)	missense	probably benign	0.26
R6923:Wnk1	UTSW	6	119,942,639 (GRCm39)	intron	probably benign
R7024:Wnk1	UTSW	6	119,942,687 (GRCm39)	intron	probably benign
R7072:Wnk1	UTSW	6	119,914,822 (GRCm39)	missense	unknown
R7087:Wnk1	UTSW	6	120,014,491 (GRCm39)	missense	possibly damaging	0.94
R7102:Wnk1	UTSW	6	119,925,268 (GRCm39)	missense	unknown
R7134:Wnk1	UTSW	6	119,903,389 (GRCm39)	missense	unknown
R7137:Wnk1	UTSW	6	120,015,173 (GRCm39)	unclassified	probably benign
R7142:Wnk1	UTSW	6	119,926,240 (GRCm39)	missense	probably benign	0.09
R7174:Wnk1	UTSW	6	119,947,939 (GRCm39)	missense	probably damaging	1.00
R7205:Wnk1	UTSW	6	119,920,839 (GRCm39)	splice site	probably null
R7218:Wnk1	UTSW	6	119,979,234 (GRCm39)	nonsense	probably null
R7498:Wnk1	UTSW	6	119,904,157 (GRCm39)	missense	unknown
R7599:Wnk1	UTSW	6	119,906,789 (GRCm39)	missense	possibly damaging	0.83
R7615:Wnk1	UTSW	6	119,909,699 (GRCm39)	missense	probably benign	0.27
R7799:Wnk1	UTSW	6	119,926,137 (GRCm39)	missense	probably benign	0.04
R7979:Wnk1	UTSW	6	120,014,409 (GRCm39)	missense	probably damaging	1.00
R8075:Wnk1	UTSW	6	119,909,675 (GRCm39)	missense	probably damaging	0.99
R8331:Wnk1	UTSW	6	119,930,794 (GRCm39)	missense	probably benign	0.09
R8343:Wnk1	UTSW	6	119,940,454 (GRCm39)	missense	probably damaging	0.99
R8348:Wnk1	UTSW	6	119,906,960 (GRCm39)	splice site	probably null
R8359:Wnk1	UTSW	6	119,969,408 (GRCm39)	missense	probably damaging	1.00
R8424:Wnk1	UTSW	6	119,911,388 (GRCm39)	missense	unknown
R8519:Wnk1	UTSW	6	119,927,044 (GRCm39)	missense	probably damaging	1.00
R8930:Wnk1	UTSW	6	119,940,226 (GRCm39)	missense	probably damaging	0.99
R8932:Wnk1	UTSW	6	119,940,226 (GRCm39)	missense	probably damaging	0.99
R8933:Wnk1	UTSW	6	120,013,959 (GRCm39)	missense	probably damaging	1.00
R9005:Wnk1	UTSW	6	119,939,393 (GRCm39)	missense	probably damaging	1.00
R9147:Wnk1	UTSW	6	119,925,631 (GRCm39)	missense	unknown
R9354:Wnk1	UTSW	6	119,942,660 (GRCm39)	missense	unknown
R9379:Wnk1	UTSW	6	119,928,678 (GRCm39)	missense	probably damaging	1.00
X0064:Wnk1	UTSW	6	120,013,993 (GRCm39)	missense	possibly damaging	0.93
Z1177:Wnk1	UTSW	6	119,925,166 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CAACAGGTTGTACCAAGGGTG -3'
(R):5'- TTATTGCAACAGGTAGCAAGC -3'

Sequencing Primer
(F):5'- CCAAGGGTGGGATATTAGGTAC -3'
(R):5'- CAATACTGCTGGGGTTGCCATAG -3'

Posted On

2022-01-20