Mutagenetix > Incidental Mutation

Incidental Mutation 'R9150:Or5k14'

694991

Institutional Source

Beutler Lab

Gene Symbol

Or5k14

Ensembl Gene

ENSMUSG00000063137

Gene Name

olfactory receptor family 5 subfamily K member 14

Synonyms

Olfr176, GA_x54KRFPKG5P-55091371-55090442, MOR184-8, GA_x54KRFPKG5P-55043245-55042289, Olfr177, MOR184-7

MMRRC Submission

068938-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.106) question?

Stock #

R9150 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

58689766-58693511 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 58693005 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 169 (C169*)

Ref Sequence

ENSEMBL: ENSMUSP00000150269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072853] [ENSMUST00000217377]

AlphaFold

E9Q7W1

Predicted Effect

probably null
Transcript: ENSMUST00000072853
AA Change: C169*

SMART Domains

Protein: ENSMUSP00000072631
Gene: ENSMUSG00000063137
AA Change: C169*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.7e-53	PFAM
Pfam:7TM_GPCR_Srsx	35	254	8.3e-6	PFAM
Pfam:7tm_1	41	290	1.2e-17	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000217377
AA Change: C169*

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

98% (56/57)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actl6b	CTCCGGTGAGTGCCTCATCC	CTCC	5: 137,553,354 (GRCm39)		probably null	Het
Ampd1	A	T	3: 102,988,359 (GRCm39)	I101F	possibly damaging	Het
Arhgap11a	A	G	2: 113,673,614 (GRCm39)	V92A	possibly damaging	Het
Arih1	C	A	9: 59,344,069 (GRCm39)	L189F	possibly damaging	Het
B4galt3	A	G	1: 171,103,899 (GRCm39)	R379G	probably benign	Het
Capn12	A	G	7: 28,590,378 (GRCm39)	N642D	probably benign	Het
Cdc42ep3	T	C	17: 79,642,299 (GRCm39)	H207R	probably benign	Het
Dcps	A	T	9: 35,035,938 (GRCm39)	L316H	probably damaging	Het
Dmgdh	A	G	13: 93,825,103 (GRCm39)	Y142C	probably damaging	Het
Elmo2	A	G	2: 165,140,607 (GRCm39)	V300A	probably damaging	Het
Eml6	A	T	11: 29,755,791 (GRCm39)	V821E	probably benign	Het
Fam181a	A	T	12: 103,282,139 (GRCm39)	N15Y	probably damaging	Het
Gm26657	G	T	4: 56,740,835 (GRCm39)	E7*	probably null	Het
Gm8267	A	T	14: 44,955,362 (GRCm39)	S220T	probably benign	Het
Gpld1	A	T	13: 25,146,305 (GRCm39)	Y183F	probably damaging	Het
Gpr158	G	A	2: 21,831,251 (GRCm39)	G784R	probably benign	Het
Grm2	A	G	9: 106,524,657 (GRCm39)	L215P		Het
Gtpbp1	A	G	15: 79,592,165 (GRCm39)	D252G	probably damaging	Het
Heatr5b	A	G	17: 79,103,448 (GRCm39)	V1135A	probably benign	Het
Irag1	T	C	7: 110,498,205 (GRCm39)	T397A	probably benign	Het
Lrrc8e	A	C	8: 4,286,030 (GRCm39)	K752Q	probably benign	Het
Ltbp2	G	A	12: 84,837,864 (GRCm39)	P1192L	probably benign	Het
Mapre2	A	G	18: 23,991,208 (GRCm39)	T201A	probably benign	Het
Mcm9	A	T	10: 53,502,110 (GRCm39)	N85K		Het
Mdga1	T	C	17: 30,057,420 (GRCm39)	T92A	probably damaging	Het
Nemf	A	C	12: 69,387,820 (GRCm39)	D421E	probably benign	Het
Oga	A	T	19: 45,771,421 (GRCm39)	V50E	probably benign	Het
Or13g1	G	C	7: 85,955,942 (GRCm39)	F126L	probably damaging	Het
Or5m3	A	T	2: 85,838,626 (GRCm39)	K169*	probably null	Het
Or8k53	A	T	2: 86,177,336 (GRCm39)	M258K	probably damaging	Het
Pcdhga5	A	C	18: 37,827,933 (GRCm39)	N127T	probably damaging	Het
Phyhipl	A	T	10: 70,404,887 (GRCm39)	V107E	probably damaging	Het
Pkd1l1	G	A	11: 8,786,256 (GRCm39)	R2383C		Het
Ppp1r16a	G	A	15: 76,575,054 (GRCm39)		probably benign	Het
Pramel20	A	G	4: 143,298,531 (GRCm39)	Y158C	probably benign	Het
Rnf41	T	C	10: 128,272,399 (GRCm39)	V142A		Het
Sgcd	C	T	11: 46,870,170 (GRCm39)	V264I	probably damaging	Het
Six2	A	G	17: 85,992,763 (GRCm39)	S247P	probably benign	Het
Slc17a7	T	G	7: 44,820,167 (GRCm39)	Y241D	probably damaging	Het
Slc30a5	T	A	13: 100,939,915 (GRCm39)	K724*	probably null	Het
Slitrk1	T	A	14: 109,149,101 (GRCm39)	I537F	possibly damaging	Het
Sp140l2	T	A	1: 85,091,359 (GRCm39)	Q33L	probably damaging	Het
Spen	C	T	4: 141,244,468 (GRCm39)	R189Q	unknown	Het
Srd5a3	T	A	5: 76,297,615 (GRCm39)	L139Q	probably damaging	Het
Tgfbrap1	A	T	1: 43,114,985 (GRCm39)	Y38*	probably null	Het
Tln2	A	T	9: 67,128,778 (GRCm39)	M1437K	probably damaging	Het
Tmem87b	T	C	2: 128,687,401 (GRCm39)	I467T	probably damaging	Het
Tnfrsf21	T	A	17: 43,398,691 (GRCm39)	I599N	probably damaging	Het
Trmt112	T	A	19: 6,887,620 (GRCm39)	L23Q	probably damaging	Het
Tsc22d1	T	A	14: 76,654,056 (GRCm39)	N178K	probably damaging	Het
Ttc23	A	G	7: 67,375,850 (GRCm39)	Y476C	probably damaging	Het
Twf1	A	T	15: 94,484,274 (GRCm39)	W83R	probably damaging	Het
Vmn2r14	T	A	5: 109,367,783 (GRCm39)	H403L	probably damaging	Het
Wiz	A	T	17: 32,586,809 (GRCm39)	I167N	probably benign	Het
Zdhhc22	G	A	12: 87,035,192 (GRCm39)	P87S	probably benign	Het
Zfp618	C	T	4: 63,039,603 (GRCm39)	Q401*	probably null	Het
Zfp715	T	A	7: 42,948,713 (GRCm39)	I416F	possibly damaging	Het

Other mutations in Or5k14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02084:Or5k14	APN	16	58,693,399 (GRCm39)	missense	probably damaging	1.00
IGL02223:Or5k14	APN	16	58,693,057 (GRCm39)	missense	probably damaging	1.00
R0131:Or5k14	UTSW	16	58,693,269 (GRCm39)	missense	probably benign	0.01
R0131:Or5k14	UTSW	16	58,693,269 (GRCm39)	missense	probably benign	0.01
R0132:Or5k14	UTSW	16	58,693,269 (GRCm39)	missense	probably benign	0.01
R0245:Or5k14	UTSW	16	58,693,229 (GRCm39)	missense	probably benign	0.01
R0717:Or5k14	UTSW	16	58,693,133 (GRCm39)	missense	probably damaging	1.00
R0975:Or5k14	UTSW	16	58,693,513 (GRCm39)	splice site	probably null
R1037:Or5k14	UTSW	16	58,693,333 (GRCm39)	missense	probably damaging	1.00
R1256:Or5k14	UTSW	16	58,693,206 (GRCm39)	nonsense	probably null
R1278:Or5k14	UTSW	16	58,693,340 (GRCm39)	missense	probably damaging	1.00
R1538:Or5k14	UTSW	16	58,693,261 (GRCm39)	missense	probably damaging	1.00
R1992:Or5k14	UTSW	16	58,692,874 (GRCm39)	missense	probably benign	0.43
R2173:Or5k14	UTSW	16	58,692,982 (GRCm39)	missense	probably damaging	0.99
R2392:Or5k14	UTSW	16	58,692,797 (GRCm39)	missense	probably damaging	1.00
R5651:Or5k14	UTSW	16	58,692,847 (GRCm39)	missense	probably damaging	0.99
R5652:Or5k14	UTSW	16	58,692,847 (GRCm39)	missense	probably damaging	0.99
R5653:Or5k14	UTSW	16	58,692,847 (GRCm39)	missense	probably damaging	0.99
R8031:Or5k14	UTSW	16	58,693,054 (GRCm39)	missense	probably benign	0.03
R8108:Or5k14	UTSW	16	58,692,599 (GRCm39)	missense	probably benign
R8531:Or5k14	UTSW	16	58,693,016 (GRCm39)	missense	probably damaging	1.00
R8833:Or5k14	UTSW	16	58,692,959 (GRCm39)	missense	probably damaging	0.99
R9318:Or5k14	UTSW	16	58,692,748 (GRCm39)	missense	probably damaging	1.00
R9389:Or5k14	UTSW	16	58,692,976 (GRCm39)	missense	probably damaging	1.00
V8831:Or5k14	UTSW	16	58,693,438 (GRCm39)	missense	probably benign	0.28

Predicted Primers

PCR Primer
(F):5'- TGCACAAGTAGATAAGGCTTTGC -3'
(R):5'- ACTACAGACTGCTTTCTTCTGG -3'

Sequencing Primer
(F):5'- CAAGTAGATAAGGCTTTGCTTCTTCC -3'
(R):5'- GCAGCCATGGCCTATGAC -3'

Posted On

2022-01-20