Incidental Mutation 'R0766:Tex22'
ID69514
Institutional Source Beutler Lab
Gene Symbol Tex22
Ensembl Gene ENSMUSG00000012211
Gene Nametestis expressed gene 22
SynonymsTep22, 1700028O09Rik
MMRRC Submission 038946-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.069) question?
Stock #R0766 (G1)
Quality Score192
Status Validated
Chromosome12
Chromosomal Location113074502-113088917 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 113088523 bp
ZygosityHeterozygous
Amino Acid Change Asparagine to Serine at position 67 (N67S)
Ref Sequence ENSEMBL: ENSMUSP00000116272 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000012355] [ENSMUST00000109729] [ENSMUST00000146107]
Predicted Effect possibly damaging
Transcript: ENSMUST00000012355
AA Change: N67S

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000012355
Gene: ENSMUSG00000012211
AA Change: N67S

DomainStartEndE-ValueType
low complexity region 14 24 N/A INTRINSIC
low complexity region 103 120 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000109729
AA Change: N67S

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000105351
Gene: ENSMUSG00000012211
AA Change: N67S

DomainStartEndE-ValueType
low complexity region 14 24 N/A INTRINSIC
low complexity region 103 120 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000146107
AA Change: N67S

PolyPhen 2 Score 0.944 (Sensitivity: 0.80; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000116272
Gene: ENSMUSG00000012211
AA Change: N67S

DomainStartEndE-ValueType
low complexity region 14 24 N/A INTRINSIC
low complexity region 103 120 N/A INTRINSIC
Meta Mutation Damage Score 0.1795 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.0%
  • 20x: 93.4%
Validation Efficiency 100% (37/37)
Allele List at MGI
Other mutations in this stock
Total: 36 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4921539E11Rik A T 4: 103,270,797 F44I probably damaging Het
A2m T C 6: 121,676,890 probably benign Het
Card14 T C 11: 119,324,176 S241P probably damaging Het
Cdh15 G A 8: 122,861,449 probably benign Het
Dnah5 A C 15: 28,448,487 K4232T probably null Het
Eml6 A G 11: 29,831,219 probably benign Het
Esd T C 14: 74,742,121 S122P probably damaging Het
Frem3 G A 8: 80,615,322 V1415I probably benign Het
Fry T C 5: 150,403,432 probably benign Het
Gp1ba C T 11: 70,641,427 P673L probably damaging Het
Herc1 C T 9: 66,504,840 P4781S probably damaging Het
Iqch G A 9: 63,482,683 S738L probably benign Het
Itih2 T A 2: 10,097,924 T800S probably benign Het
Itpr1 A G 6: 108,410,900 E1533G probably damaging Het
Klrg1 T C 6: 122,279,663 M55V probably benign Het
Lrrk2 A G 15: 91,699,895 N286S probably damaging Het
Mkx T A 18: 6,937,192 D284V probably benign Het
Mroh2a C T 1: 88,230,680 R150* probably null Het
Otos A C 1: 92,645,351 L14R probably damaging Het
Plch2 C T 4: 154,989,799 V765M probably damaging Het
Ppp4r3b A T 11: 29,173,358 Q18L probably benign Het
Psme4 T A 11: 30,807,687 probably null Het
Pwp1 G A 10: 85,879,309 D220N probably damaging Het
Rel G A 11: 23,757,010 T64I probably damaging Het
Snai2 T A 16: 14,708,247 M254K possibly damaging Het
Sntb2 A G 8: 107,001,577 T386A probably damaging Het
Tedc2 T A 17: 24,216,317 E366V probably damaging Het
Tedc2 C A 17: 24,216,318 E366* probably null Het
Trank1 T G 9: 111,347,469 S270A probably benign Het
Ttc30a2 A T 2: 75,976,332 V612D probably benign Het
Vcp T C 4: 42,988,728 T249A possibly damaging Het
Vmn1r167 A G 7: 23,505,123 F156S probably benign Het
Vrk2 G A 11: 26,535,522 probably benign Het
Wdfy4 T C 14: 33,140,612 E601G probably damaging Het
Zfp407 C T 18: 84,559,773 A1072T probably benign Het
Zfp638 A G 6: 83,929,041 N63D probably damaging Het
Other mutations in Tex22
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0100:Tex22 UTSW 12 113088772 missense probably benign 0.15
R0100:Tex22 UTSW 12 113088772 missense probably benign 0.15
R1497:Tex22 UTSW 12 113075380 missense probably benign 0.00
R4761:Tex22 UTSW 12 113088766 missense possibly damaging 0.54
R5013:Tex22 UTSW 12 113088484 missense probably damaging 0.97
R7859:Tex22 UTSW 12 113088483 missense possibly damaging 0.92
R7942:Tex22 UTSW 12 113088483 missense possibly damaging 0.92
Z1177:Tex22 UTSW 12 113088619 frame shift probably null
Predicted Primers PCR Primer
(F):5'- ACCATGTCGGGCTACTGTGAGAAG -3'
(R):5'- GCCAAGAAGTCCTGGAAGGCATTAG -3'

Sequencing Primer
(F):5'- CTACTGTGAGAAGGTACTGCTTCC -3'
(R):5'- GGGATCAGCCATAGTCTCACAG -3'
Posted On2013-09-30