Incidental Mutation 'R9155:Or2n1d'
ID 695341
Institutional Source Beutler Lab
Gene Symbol Or2n1d
Ensembl Gene ENSMUSG00000096840
Gene Name olfactory receptor family 2 subfamily N member 1D
Synonyms Olfr136, MOR256-7, GA_x6K02T2PSCP-2779375-2780313
MMRRC Submission 068941-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.127) question?
Stock # R9155 (G1)
Quality Score 225.009
Status Not validated
Chromosome 17
Chromosomal Location 38646050-38646988 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 38646224 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Methionine to Leucine at position 59 (M59L)
Ref Sequence ENSEMBL: ENSMUSP00000146828 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000077203] [ENSMUST00000208525] [ENSMUST00000208539] [ENSMUST00000214035] [ENSMUST00000216963]
AlphaFold Q8VG72
Predicted Effect probably damaging
Transcript: ENSMUST00000077203
AA Change: M59L

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000076443
Gene: ENSMUSG00000096840
AA Change: M59L

DomainStartEndE-ValueType
Pfam:7tm_4 31 308 2.6e-48 PFAM
Pfam:7tm_1 41 290 7.1e-25 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000208525
AA Change: M59L

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
Predicted Effect probably damaging
Transcript: ENSMUST00000208539
AA Change: M59L

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
Predicted Effect probably damaging
Transcript: ENSMUST00000214035
AA Change: M59L

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
Predicted Effect probably damaging
Transcript: ENSMUST00000216963
AA Change: M59L

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 71 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700028K03Rik A G 5: 107,691,811 (GRCm39) E34G probably damaging Het
Aldh1a3 A T 7: 66,058,867 (GRCm39) L157* probably null Het
Asic2 T A 11: 80,784,872 (GRCm39) T356S probably benign Het
Ate1 T C 7: 129,996,463 (GRCm39) D459G probably damaging Het
Cacna1g T A 11: 94,350,423 (GRCm39) Q474L Het
Calhm6 T C 10: 34,002,363 (GRCm39) E240G probably damaging Het
Carf C A 1: 60,189,842 (GRCm39) T689K possibly damaging Het
Carmil2 A G 8: 106,412,922 (GRCm39) D6G probably benign Het
Ccnd2 A C 6: 127,127,663 (GRCm39) V25G probably damaging Het
Ccnk T A 12: 108,159,978 (GRCm39) F153L probably damaging Het
Cdh23 C T 10: 60,249,485 (GRCm39) G808E probably damaging Het
Clec11a C T 7: 43,954,317 (GRCm39) R212Q probably damaging Het
Cog4 T C 8: 111,608,384 (GRCm39) W749R probably damaging Het
Col6a3 T A 1: 90,738,301 (GRCm39) T1073S probably benign Het
Col6a4 T A 9: 105,952,209 (GRCm39) D563V probably benign Het
Coq5 A G 5: 115,433,839 (GRCm39) probably null Het
Crebbp A C 16: 3,914,346 (GRCm39) H1292Q probably damaging Het
Csmd3 C A 15: 47,449,051 (GRCm39) G2737W Het
Ddrgk1 T C 2: 130,500,227 (GRCm39) Y223C probably damaging Het
Dennd1c T C 17: 57,373,796 (GRCm39) Q589R probably benign Het
Dnah10 A G 5: 124,907,475 (GRCm39) D4336G probably damaging Het
Dnah11 C T 12: 117,991,251 (GRCm39) E2372K probably damaging Het
E330034G19Rik A T 14: 24,346,938 (GRCm39) Q140L possibly damaging Het
Ergic3 A G 2: 155,850,780 (GRCm39) Y83C probably damaging Het
Fam171a2 A T 11: 102,329,497 (GRCm39) S421T probably benign Het
Fndc3a T A 14: 72,921,162 (GRCm39) H4L possibly damaging Het
Gid8 T A 2: 180,359,756 (GRCm39) Y213* probably null Het
Hephl1 G T 9: 15,000,375 (GRCm39) H292Q probably damaging Het
Hexb T C 13: 97,314,414 (GRCm39) Y443C probably damaging Het
Htr1f C T 16: 64,746,788 (GRCm39) R168H probably benign Het
Hus1 A G 11: 8,956,056 (GRCm39) I159T probably damaging Het
Inha A G 1: 75,486,133 (GRCm39) T143A probably benign Het
Itga8 A G 2: 12,194,330 (GRCm39) I690T probably benign Het
Itgae G T 11: 73,016,089 (GRCm39) C766F possibly damaging Het
Kank4 T C 4: 98,666,563 (GRCm39) E628G probably benign Het
Kctd19 T C 8: 106,120,571 (GRCm39) H221R probably benign Het
Llgl1 A G 11: 60,597,934 (GRCm39) E351G probably benign Het
Lrba A G 3: 86,202,508 (GRCm39) Y253C probably damaging Het
Lrp2 T A 2: 69,291,713 (GRCm39) R3489* probably null Het
Lrriq1 T C 10: 103,050,640 (GRCm39) N704S probably benign Het
Mbtps1 T C 8: 120,235,693 (GRCm39) N995S probably benign Het
Mga T C 2: 119,757,013 (GRCm39) C1077R probably damaging Het
Muc21 G A 17: 35,932,131 (GRCm39) P685L unknown Het
Ndufv1 A T 19: 4,059,912 (GRCm39) C142S probably damaging Het
Nkx3-1 T C 14: 69,429,660 (GRCm39) L226P probably damaging Het
Nsd1 C T 13: 55,361,253 (GRCm39) R74W probably damaging Het
Or12k8 A C 2: 36,975,016 (GRCm39) M248R probably benign Het
Or1d2 A G 11: 74,255,791 (GRCm39) T99A probably benign Het
Or5b99 G A 19: 12,976,428 (GRCm39) C26Y probably benign Het
Or7g35 A T 9: 19,496,379 (GRCm39) D182V probably benign Het
Phc3 A G 3: 30,968,691 (GRCm39) V812A probably benign Het
Pigt CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT 2: 164,341,589 (GRCm39) probably null Het
Ppp4c A T 7: 126,386,419 (GRCm39) C193S possibly damaging Het
Rbbp5 C A 1: 132,422,023 (GRCm39) P308T probably damaging Het
Rhot1 A G 11: 80,148,380 (GRCm39) T607A probably null Het
Secisbp2l G A 2: 125,617,623 (GRCm39) P18L probably damaging Het
Slc27a4 G A 2: 29,701,294 (GRCm39) G362S probably damaging Het
Slc4a8 T C 15: 100,672,571 (GRCm39) Y36H probably damaging Het
Sox17 T C 1: 4,562,447 (GRCm39) Y251C probably damaging Het
Spata31h1 A T 10: 82,120,203 (GRCm39) I4269N probably damaging Het
Taf4b T C 18: 14,946,296 (GRCm39) V373A probably benign Het
Tecpr2 T A 12: 110,881,184 (GRCm39) V107E probably damaging Het
Them7 A G 2: 105,209,124 (GRCm39) Y148C probably damaging Het
Tktl2 T A 8: 66,965,858 (GRCm39) M472K possibly damaging Het
Ttn C T 2: 76,625,937 (GRCm39) V15041I probably damaging Het
Ubr1 A G 2: 120,754,615 (GRCm39) V751A possibly damaging Het
Vmn1r213 A T 13: 23,196,343 (GRCm39) R309* probably null Het
Vmn2r10 A T 5: 109,144,212 (GRCm39) D579E probably benign Het
Vmn2r118 T C 17: 55,917,207 (GRCm39) Q435R probably null Het
Vmn2r50 A T 7: 9,781,571 (GRCm39) H391Q probably damaging Het
Zbtb44 A T 9: 30,965,309 (GRCm39) I240F probably benign Het
Other mutations in Or2n1d
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01712:Or2n1d APN 17 38,646,848 (GRCm39) missense probably benign 0.00
IGL01787:Or2n1d APN 17 38,646,470 (GRCm39) missense probably damaging 0.98
IGL02480:Or2n1d APN 17 38,646,314 (GRCm39) missense probably benign 0.32
IGL02603:Or2n1d APN 17 38,646,404 (GRCm39) missense probably damaging 1.00
IGL03122:Or2n1d APN 17 38,646,192 (GRCm39) missense probably benign 0.01
BB009:Or2n1d UTSW 17 38,646,146 (GRCm39) missense probably benign 0.01
BB019:Or2n1d UTSW 17 38,646,146 (GRCm39) missense probably benign 0.01
R0295:Or2n1d UTSW 17 38,646,182 (GRCm39) missense probably damaging 1.00
R0684:Or2n1d UTSW 17 38,646,735 (GRCm39) missense probably benign 0.11
R1874:Or2n1d UTSW 17 38,646,860 (GRCm39) missense probably damaging 1.00
R3436:Or2n1d UTSW 17 38,646,323 (GRCm39) missense probably damaging 1.00
R3437:Or2n1d UTSW 17 38,646,323 (GRCm39) missense probably damaging 1.00
R4714:Or2n1d UTSW 17 38,646,731 (GRCm39) missense possibly damaging 0.65
R4715:Or2n1d UTSW 17 38,646,731 (GRCm39) missense possibly damaging 0.65
R4716:Or2n1d UTSW 17 38,646,731 (GRCm39) missense possibly damaging 0.65
R4878:Or2n1d UTSW 17 38,646,518 (GRCm39) missense probably benign
R5296:Or2n1d UTSW 17 38,646,347 (GRCm39) nonsense probably null
R5370:Or2n1d UTSW 17 38,646,335 (GRCm39) nonsense probably null
R5413:Or2n1d UTSW 17 38,646,515 (GRCm39) missense probably benign 0.03
R5988:Or2n1d UTSW 17 38,646,911 (GRCm39) missense probably damaging 1.00
R6156:Or2n1d UTSW 17 38,646,064 (GRCm39) missense probably damaging 0.99
R6550:Or2n1d UTSW 17 38,646,896 (GRCm39) missense possibly damaging 0.65
R7395:Or2n1d UTSW 17 38,646,755 (GRCm39) nonsense probably null
R7417:Or2n1d UTSW 17 38,646,183 (GRCm39) missense probably damaging 1.00
R7746:Or2n1d UTSW 17 38,646,285 (GRCm39) missense probably benign 0.16
R7747:Or2n1d UTSW 17 38,646,285 (GRCm39) missense probably benign 0.16
R7821:Or2n1d UTSW 17 38,646,855 (GRCm39) missense probably benign 0.13
R7932:Or2n1d UTSW 17 38,646,146 (GRCm39) missense probably benign 0.01
R8409:Or2n1d UTSW 17 38,646,197 (GRCm39) missense probably benign 0.09
R8911:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R8912:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R8913:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R8914:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R8968:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9006:Or2n1d UTSW 17 38,646,723 (GRCm39) missense possibly damaging 0.84
R9044:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9110:Or2n1d UTSW 17 38,646,434 (GRCm39) missense probably damaging 1.00
R9279:Or2n1d UTSW 17 38,646,414 (GRCm39) missense probably damaging 0.99
R9289:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9295:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9317:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9318:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9348:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9409:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9410:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9411:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9412:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9413:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9512:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9522:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
R9524:Or2n1d UTSW 17 38,646,540 (GRCm39) nonsense probably null
R9547:Or2n1d UTSW 17 38,646,341 (GRCm39) missense possibly damaging 0.80
R9580:Or2n1d UTSW 17 38,646,320 (GRCm39) missense possibly damaging 0.94
V5088:Or2n1d UTSW 17 38,646,050 (GRCm39) start codon destroyed probably null 0.99
Z1176:Or2n1d UTSW 17 38,646,243 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- TGGCTGTAACCAATGAAAGCC -3'
(R):5'- CACTCTTTGGTTCATGATGAGG -3'

Sequencing Primer
(F):5'- CACCCAAAAGAATTCATCCTACTGGG -3'
(R):5'- GGTTTGCAGATAGCCACATATCG -3'
Posted On 2022-01-20