Mutagenetix > Incidental Mutation

Incidental Mutation 'R9163:Vmn2r23'

695836

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r23

Ensembl Gene

ENSMUSG00000091620

Gene Name

vomeronasal 2, receptor 23

Synonyms

EG435916

MMRRC Submission

068975-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R9163 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

123702821-123742291 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 123741823 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 712 (C712S)

Ref Sequence

ENSEMBL: ENSMUSP00000126682 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172391]

AlphaFold

E9PXI5

Predicted Effect

probably damaging
Transcript: ENSMUST00000172391
AA Change: C712S

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000126682
Gene: ENSMUSG00000091620
AA Change: C712S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	79	461	1.7e-31	PFAM
Pfam:NCD3G	513	566	1.2e-23	PFAM
Pfam:7tm_3	596	834	1.5e-55	PFAM

Meta Mutation Damage Score

0.6329

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

99% (69/70)

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	T	C	13: 77,245,281	S351P	possibly damaging	Het
4930558K02Rik	A	G	1: 161,949,605	S128P	probably benign	Het
Adam17	C	T	12: 21,351,587	V136I	probably benign	Het
Adam19	A	G	11: 46,127,349	T413A	probably benign	Het
Adam28	T	C	14: 68,629,082	K443E	probably damaging	Het
Adgrv1	T	A	13: 81,419,422	T5211S	probably benign	Het
Adnp2	A	T	18: 80,128,985	N736K	possibly damaging	Het
Agtpbp1	C	T	13: 59,462,070	W1082*	probably null	Het
Ap3b2	T	G	7: 81,463,798	M888L	unknown	Het
Arhgap35	C	A	7: 16,561,624	R1172L	possibly damaging	Het
Arrdc3	T	A	13: 80,893,387	D402E	probably benign	Het
Ccdc14	T	C	16: 34,690,748	F31L	possibly damaging	Het
Ccdc173	A	T	2: 69,776,166	I302K	probably benign	Het
Cd5l	T	G	3: 87,367,625	L157R	probably benign	Het
Cdc14a	G	T	3: 116,328,564	D251E	possibly damaging	Het
Chmp2a	T	A	7: 13,032,718	Q91L	possibly damaging	Het
Col12a1	A	G	9: 79,641,447		probably null	Het
Ctdsp1	G	T	1: 74,394,967	R201L	possibly damaging	Het
Dhx15	C	T	5: 52,184,856	R40H	probably damaging	Het
Dnph1	A	G	17: 46,498,934	Y134C	probably damaging	Het
Foxo1	T	A	3: 52,345,880	V488D	probably benign	Het
Gcn1l1	C	A	5: 115,604,885	N1538K	probably benign	Het
Gldc	T	A	19: 30,134,286	E500V	probably benign	Het
Hhip	G	A	8: 79,975,114	T597I	probably benign	Het
Hmgn1	A	G	16: 96,127,309		probably null	Het
Ifi205	A	T	1: 174,017,422	S265T	possibly damaging	Het
Igkv1-131	T	C	6: 67,766,769	M2V	probably benign	Het
Irgm2	T	G	11: 58,220,454	S324A	probably damaging	Het
Jarid2	C	G	13: 44,911,251	D937E	possibly damaging	Het
Jrkl	A	T	9: 13,245,399	W86R	probably damaging	Het
Kbtbd3	A	G	9: 4,330,584	I319M	probably benign	Het
Klhl7	T	C	5: 24,159,467	Y500H	probably damaging	Het
Lats1	C	T	10: 7,702,288	A392V	probably benign	Het
Llgl1	G	T	11: 60,709,576	A584S	probably benign	Het
Macf1	G	T	4: 123,509,893	S560Y	probably damaging	Het
Olfr1206	C	T	2: 88,865,451	P282L	possibly damaging	Het
Olfr250	T	A	9: 38,368,082	C169S	probably damaging	Het
Pde10a	G	A	17: 8,962,959	C498Y	possibly damaging	Het
Pde4dip	A	G	3: 97,751,807		probably null	Het
Pdhx	G	A	2: 103,022,216	P434L	probably damaging	Het
Pdlim3	T	C	8: 45,885,674		probably null	Het
Pik3c3	T	C	18: 30,294,430		probably null	Het
Prkag1	T	C	15: 98,814,019	K242R	possibly damaging	Het
Rap1b	A	G	10: 117,818,486	S150P	possibly damaging	Het
Scn4a	A	G	11: 106,326,250	S1102P	probably damaging	Het
Scrib	A	G	15: 76,049,259	Y1332H	probably damaging	Het
Sim1	A	G	10: 50,896,069	E58G	probably benign	Het
Slc13a1	C	T	6: 24,097,579		probably null	Het
Slc17a8	A	G	10: 89,589,582	V380A	probably damaging	Het
Slc5a4b	G	T	10: 76,081,331	Y290*	probably null	Het
Slco4c1	A	G	1: 96,836,908	F480L	probably damaging	Het
Srcap	T	C	7: 127,521,990	V146A	unknown	Het
Ssc5d	T	A	7: 4,933,433	D559E	probably damaging	Het
Sstr5	A	C	17: 25,491,610	I215S	probably damaging	Het
Stambpl1	G	A	19: 34,235,234	C268Y	probably benign	Het
Stard3nl	C	A	13: 19,376,639		probably benign	Het
Sycp3	T	C	10: 88,463,872		probably null	Het
Syne2	A	T	12: 75,962,575	M2417L	possibly damaging	Het
Tenm4	T	G	7: 96,823,873	Y959D	probably damaging	Het
Tmem132d	T	A	5: 127,792,506	D588V	possibly damaging	Het
Tnfrsf26	T	C	7: 143,618,393	E60G	possibly damaging	Het
Topbp1	A	G	9: 103,328,568	R802G	probably benign	Het
Trmu	T	C	15: 85,896,895	V360A	probably benign	Het
Tshz2	G	A	2: 169,884,642	C386Y	probably damaging	Het
Ttn	A	T	2: 76,870,427	D61E		Het
Upf1	T	C	8: 70,340,024	T345A	probably benign	Het
Vmn2r44	C	T	7: 8,368,092	V652I	probably benign	Het
Wfdc1	T	C	8: 119,666,563	V8A	probably benign	Het
Xrn1	A	G	9: 95,998,221	Y738C	probably benign	Het
Zfp618	T	G	4: 63,133,274	V764G	probably damaging	Het
Znfx1	A	T	2: 167,056,341	M221K	probably damaging	Het

Other mutations in Vmn2r23

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00324:Vmn2r23	APN	6	123729725	missense	possibly damaging	0.89
IGL01012:Vmn2r23	APN	6	123729596	missense	probably benign
IGL01073:Vmn2r23	APN	6	123712800	missense	possibly damaging	0.82
IGL01547:Vmn2r23	APN	6	123704424	missense	possibly damaging	0.88
IGL01571:Vmn2r23	APN	6	123704407	missense	probably damaging	1.00
IGL01950:Vmn2r23	APN	6	123741886	missense	possibly damaging	0.80
IGL02028:Vmn2r23	APN	6	123741860	missense	probably damaging	1.00
IGL02248:Vmn2r23	APN	6	123741744	missense	probably damaging	0.96
IGL02318:Vmn2r23	APN	6	123741836	missense	probably benign	0.10
IGL02649:Vmn2r23	APN	6	123704478	missense	probably benign
IGL02831:Vmn2r23	APN	6	123704385	missense	probably benign	0.22
IGL02832:Vmn2r23	APN	6	123704396	missense	probably benign	0.00
IGL02865:Vmn2r23	APN	6	123741619	missense	probably damaging	1.00
IGL02964:Vmn2r23	APN	6	123741782	missense	possibly damaging	0.93
IGL03347:Vmn2r23	APN	6	123704374	missense	probably benign	0.01
IGL03396:Vmn2r23	APN	6	123729626	missense	probably damaging	1.00
PIT4472001:Vmn2r23	UTSW	6	123712977	missense	possibly damaging	0.62
R0597:Vmn2r23	UTSW	6	123729721	missense	probably benign	0.08
R0677:Vmn2r23	UTSW	6	123713451	missense	probably benign	0.00
R0904:Vmn2r23	UTSW	6	123742135	missense	probably damaging	1.00
R1330:Vmn2r23	UTSW	6	123742004	missense	probably damaging	1.00
R1424:Vmn2r23	UTSW	6	123713270	nonsense	probably null
R1629:Vmn2r23	UTSW	6	123713427	missense	probably benign	0.05
R1842:Vmn2r23	UTSW	6	123729690	missense	possibly damaging	0.77
R1867:Vmn2r23	UTSW	6	123702915	missense	probably damaging	1.00
R1919:Vmn2r23	UTSW	6	123713010	missense	possibly damaging	0.94
R2087:Vmn2r23	UTSW	6	123741499	missense	probably benign	0.00
R2338:Vmn2r23	UTSW	6	123704425	missense	possibly damaging	0.88
R2568:Vmn2r23	UTSW	6	123742188	nonsense	probably null
R2867:Vmn2r23	UTSW	6	123713164	missense	possibly damaging	0.94
R2867:Vmn2r23	UTSW	6	123713164	missense	possibly damaging	0.94
R3500:Vmn2r23	UTSW	6	123713170	missense	possibly damaging	0.81
R3789:Vmn2r23	UTSW	6	123741389	missense	probably damaging	1.00
R4164:Vmn2r23	UTSW	6	123729738	missense	probably benign
R4506:Vmn2r23	UTSW	6	123702925	missense	probably damaging	1.00
R4652:Vmn2r23	UTSW	6	123741730	missense	probably damaging	1.00
R4697:Vmn2r23	UTSW	6	123741826	missense	probably damaging	1.00
R4840:Vmn2r23	UTSW	6	123713074	missense	probably damaging	1.00
R4983:Vmn2r23	UTSW	6	123733349	missense	probably damaging	1.00
R5276:Vmn2r23	UTSW	6	123712977	missense	possibly damaging	0.62
R5392:Vmn2r23	UTSW	6	123704364	missense	probably benign	0.36
R5528:Vmn2r23	UTSW	6	123713002	missense	probably damaging	1.00
R5529:Vmn2r23	UTSW	6	123713451	missense	probably benign	0.00
R5664:Vmn2r23	UTSW	6	123713074	missense	probably damaging	1.00
R5749:Vmn2r23	UTSW	6	123733273	missense	probably benign
R5761:Vmn2r23	UTSW	6	123712759	missense	probably benign	0.39
R5762:Vmn2r23	UTSW	6	123733393	missense	probably damaging	1.00
R5868:Vmn2r23	UTSW	6	123712942	missense	probably benign	0.12
R5935:Vmn2r23	UTSW	6	123741895	missense	possibly damaging	0.94
R6242:Vmn2r23	UTSW	6	123704400	missense	possibly damaging	0.82
R6416:Vmn2r23	UTSW	6	123712902	missense	probably damaging	1.00
R6524:Vmn2r23	UTSW	6	123713425	missense	probably damaging	1.00
R6576:Vmn2r23	UTSW	6	123733273	missense	probably benign
R6925:Vmn2r23	UTSW	6	123704553	missense	probably damaging	1.00
R7148:Vmn2r23	UTSW	6	123713022	missense	probably benign
R7215:Vmn2r23	UTSW	6	123704364	missense	probably benign	0.36
R7252:Vmn2r23	UTSW	6	123741581	missense	probably damaging	0.97
R7403:Vmn2r23	UTSW	6	123704579	missense	probably benign	0.01
R8015:Vmn2r23	UTSW	6	123704541	missense	probably benign	0.00
R8143:Vmn2r23	UTSW	6	123741353	missense	probably damaging	0.99
R8474:Vmn2r23	UTSW	6	123704640	missense	probably benign	0.36
R8520:Vmn2r23	UTSW	6	123741656	missense	probably damaging	0.99
R8679:Vmn2r23	UTSW	6	123713472	missense	probably damaging	0.99
R8713:Vmn2r23	UTSW	6	123703032	missense
R8966:Vmn2r23	UTSW	6	123742120	missense	possibly damaging	0.94
R9124:Vmn2r23	UTSW	6	123742079	missense	possibly damaging	0.57
R9189:Vmn2r23	UTSW	6	123704364	missense	probably benign	0.36
R9451:Vmn2r23	UTSW	6	123733393	missense	probably damaging	1.00
R9495:Vmn2r23	UTSW	6	123712713	missense	probably benign	0.30
R9514:Vmn2r23	UTSW	6	123712713	missense	probably benign	0.30
RF018:Vmn2r23	UTSW	6	123713116	missense	probably benign	0.00
T0975:Vmn2r23	UTSW	6	123713161	missense	probably benign	0.00
Z1088:Vmn2r23	UTSW	6	123742108	missense	probably damaging	0.98
Z1177:Vmn2r23	UTSW	6	123729725	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- AATATTCATTGGCCAACCTGGAAC -3'
(R):5'- TGTCAGGTAGCCTTCTCACCAG -3'

Sequencing Primer
(F):5'- GAACAGTCACTTGTGTCTTGAGAC -3'
(R):5'- GGTAGCCTTCTCACCAGAAAAG -3'

Posted On

2022-02-07