Mutagenetix > Incidental Mutation

Incidental Mutation 'R9167:Tmem266'

696131

Institutional Source

Beutler Lab

Gene Symbol

Tmem266

Ensembl Gene

ENSMUSG00000032313

Gene Name

transmembrane protein 266

Synonyms

AI118078

MMRRC Submission

068946-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.086) question?

Stock #

R9167 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

55234197-55345629 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 55322231 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 174 (D174G)

Ref Sequence

ENSEMBL: ENSMUSP00000034862 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034862] [ENSMUST00000085754]

AlphaFold

Q8BZB3

Predicted Effect

probably damaging
Transcript: ENSMUST00000034862
AA Change: D174G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000034862
Gene: ENSMUSG00000032313
AA Change: D174G

Domain	Start	End	E-Value	Type
low complexity region	16	27	N/A	INTRINSIC
transmembrane domain	95	117	N/A	INTRINSIC
transmembrane domain	137	159	N/A	INTRINSIC
transmembrane domain	172	191	N/A	INTRINSIC
low complexity region	260	283	N/A	INTRINSIC
low complexity region	298	309	N/A	INTRINSIC
low complexity region	381	396	N/A	INTRINSIC
low complexity region	423	439	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000085754
AA Change: D109G

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000082906
Gene: ENSMUSG00000032313
AA Change: D109G

Domain	Start	End	E-Value	Type
transmembrane domain	30	52	N/A	INTRINSIC
transmembrane domain	72	94	N/A	INTRINSIC
low complexity region	195	218	N/A	INTRINSIC
low complexity region	233	244	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

100% (44/44)

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	C	A	11: 119,901,952 (GRCm39)	V815F	possibly damaging	Het
Abcb4	T	A	5: 8,986,849 (GRCm39)	L756*	probably null	Het
Acrbp	T	C	6: 125,039,942 (GRCm39)	L515P	probably damaging	Het
Amot	A	T	X: 144,244,745 (GRCm39)	L435H		Het
Atic	T	A	1: 71,604,040 (GRCm39)	I201N	probably benign	Het
Avpr1b	T	C	1: 131,537,151 (GRCm39)	S312P	probably damaging	Het
Cables1	T	C	18: 12,039,029 (GRCm39)	V314A	possibly damaging	Het
Cd22	G	A	7: 30,575,430 (GRCm39)	T204I	probably benign	Het
Cep250	A	G	2: 155,828,920 (GRCm39)	N95D		Het
Ctla4	C	T	1: 60,951,695 (GRCm39)	R75W	probably damaging	Het
Cul7	A	G	17: 46,966,623 (GRCm39)	M649V	probably benign	Het
Ddx46	T	A	13: 55,802,915 (GRCm39)	V479E	probably null	Het
Dnah7a	T	C	1: 53,657,370 (GRCm39)	N802S	probably benign	Het
Fmn2	G	T	1: 174,331,056 (GRCm39)	R482L	unknown	Het
Fyn	T	G	10: 39,402,811 (GRCm39)	D194E	probably benign	Het
Hdac4	G	A	1: 91,875,256 (GRCm39)	T905M	probably benign	Het
Herc1	A	G	9: 66,411,900 (GRCm39)	I4707V	possibly damaging	Het
Hmgcs2	T	C	3: 98,204,430 (GRCm39)	I277T	possibly damaging	Het
Kcnj15	A	G	16: 95,096,741 (GRCm39)	D121G	probably damaging	Het
Kcnt2	A	G	1: 140,506,200 (GRCm39)	E899G	probably benign	Het
Kif16b	A	T	2: 142,542,840 (GRCm39)	L152*	probably null	Het
Krt73	A	G	15: 101,702,405 (GRCm39)	V486A	probably benign	Het
Lrrc37a	T	A	11: 103,347,658 (GRCm39)	K3012N	unknown	Het
Musk	A	T	4: 58,296,687 (GRCm39)	I96F	probably benign	Het
Myrfl	A	G	10: 116,667,450 (GRCm39)	V330A	probably damaging	Het
Nanp	A	T	2: 150,872,728 (GRCm39)	C67S	probably benign	Het
Nlrp4e	A	G	7: 23,039,951 (GRCm39)	N758S	probably benign	Het
Nrxn3	T	A	12: 89,154,068 (GRCm39)	V345D	probably damaging	Het
Nsun7	G	A	5: 66,435,994 (GRCm39)	V289I	possibly damaging	Het
Or52d1	A	G	7: 103,756,426 (GRCm39)	*313W	probably null	Het
Pde4a	T	C	9: 21,102,798 (GRCm39)	S161P	probably benign	Het
Pom121l2	C	T	13: 22,167,160 (GRCm39)	A477V	probably damaging	Het
Rest	GGGGGCCTGCCCCTCCCACGGGGCCTGCCCCTCCCACGGGGCCTGCCCCTCCCACGGAGCCTGCCCCTCCCACGGGGC	GGGGGCCTGCCCCTCCCACGGGGCCTGCCCCTCCCACGGAGCCTGCCCCTCCCACGGGGC	5: 77,429,651 (GRCm39)		probably benign	Het
Ryr3	A	T	2: 112,664,398 (GRCm39)	I1651N	probably damaging	Het
Serpinb6e	A	G	13: 34,023,009 (GRCm39)	F125L	possibly damaging	Het
Slc2a9	T	A	5: 38,549,092 (GRCm39)	T341S	probably damaging	Het
Taf3	A	G	2: 9,945,804 (GRCm39)	V600A	probably benign	Het
Tasor2	A	G	13: 3,624,724 (GRCm39)	V1742A	probably benign	Het
Tlx2	T	A	6: 83,046,042 (GRCm39)	K211*	probably null	Het
Tpm3	T	A	3: 89,994,824 (GRCm39)	I147N	probably benign	Het
Unc93a	T	C	17: 13,335,108 (GRCm39)	K313E	possibly damaging	Het
Vmn1r9	A	C	6: 57,048,138 (GRCm39)	E71A	probably benign	Het
Vwa2	T	A	19: 56,899,063 (GRCm39)	D754E	probably benign	Het
Zfp677	T	C	17: 21,613,460 (GRCm39)	V18A	probably damaging	Het

Other mutations in Tmem266

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01726:Tmem266	APN	9	55,342,486 (GRCm39)	missense	probably benign	0.12
IGL03366:Tmem266	APN	9	55,344,517 (GRCm39)	missense	probably benign	0.06
R0088:Tmem266	UTSW	9	55,344,613 (GRCm39)	missense	probably damaging	1.00
R0418:Tmem266	UTSW	9	55,344,697 (GRCm39)	missense	probably benign	0.06
R0497:Tmem266	UTSW	9	55,288,168 (GRCm39)	splice site	probably null
R0763:Tmem266	UTSW	9	55,322,239 (GRCm39)	missense	probably damaging	1.00
R1657:Tmem266	UTSW	9	55,325,292 (GRCm39)	missense	probably damaging	1.00
R3617:Tmem266	UTSW	9	55,307,918 (GRCm39)	missense	probably damaging	0.99
R4235:Tmem266	UTSW	9	55,325,391 (GRCm39)	missense	probably damaging	0.99
R4767:Tmem266	UTSW	9	55,288,025 (GRCm39)	missense	probably damaging	1.00
R5587:Tmem266	UTSW	9	55,344,850 (GRCm39)	missense	probably damaging	1.00
R5732:Tmem266	UTSW	9	55,288,120 (GRCm39)	missense	probably damaging	1.00
R5783:Tmem266	UTSW	9	55,305,087 (GRCm39)	missense	probably damaging	1.00
R6193:Tmem266	UTSW	9	55,344,493 (GRCm39)	missense	probably benign	0.01
R6943:Tmem266	UTSW	9	55,284,851 (GRCm39)	start gained	probably benign
R7459:Tmem266	UTSW	9	55,303,883 (GRCm39)	missense	unknown
R9502:Tmem266	UTSW	9	55,344,973 (GRCm39)	missense	probably damaging	0.96
X0063:Tmem266	UTSW	9	55,288,033 (GRCm39)	missense	probably benign	0.00
Z1177:Tmem266	UTSW	9	55,344,754 (GRCm39)	missense	possibly damaging	0.70
Z1177:Tmem266	UTSW	9	55,305,051 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCCCTCAGGTTGTCTAAAC -3'
(R):5'- TTTCTCAAGTGGACAGCCTCC -3'

Sequencing Primer
(F):5'- GCCCTCAGGTTGTCTAAACTAAGAG -3'
(R):5'- CAGCTTCTGCCTCCTCACCG -3'

Posted On

2022-02-07