Mutagenetix > Incidental Mutation

Incidental Mutation 'R9172:Mroh4'

696503

Institutional Source

Beutler Lab

Gene Symbol

Mroh4

Ensembl Gene

ENSMUSG00000022603

Gene Name

maestro heat-like repeat family member 4

Synonyms

1700016M24Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9172 (G1)

Quality Score

176.009

Status

Validated

Chromosome

Chromosomal Location

74606029-74636353 bp(-) (GRCm38)

Type of Mutation

makesense

DNA Base Change (assembly)

T to C at 74606112 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Tryptophan at position 984 (*984W)

Ref Sequence

ENSEMBL: ENSMUSP00000023271 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023271] [ENSMUST00000137963]

AlphaFold

G3X8W1

Predicted Effect

probably null
Transcript: ENSMUST00000023271
AA Change: *984W

SMART Domains

Protein: ENSMUSP00000023271
Gene: ENSMUSG00000022603
AA Change: *984W

Domain	Start	End	E-Value	Type
low complexity region	1	12	N/A	INTRINSIC
low complexity region	326	337	N/A	INTRINSIC
low complexity region	428	435	N/A	INTRINSIC
low complexity region	520	534	N/A	INTRINSIC
low complexity region	572	591	N/A	INTRINSIC
SCOP:d1ee4a_	709	852	3e-5	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000137963
AA Change: *915W

SMART Domains

Protein: ENSMUSP00000117011
Gene: ENSMUSG00000022603
AA Change: *915W

Domain	Start	End	E-Value	Type
low complexity region	257	268	N/A	INTRINSIC
low complexity region	359	366	N/A	INTRINSIC
low complexity region	451	465	N/A	INTRINSIC
low complexity region	503	522	N/A	INTRINSIC
SCOP:d1ee4a_	640	783	3e-5	SMART

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130019O22Rik	T	C	7: 127,385,454	K159E	probably benign	Het
Accsl	A	T	2: 93,861,488	L332Q	probably damaging	Het
Adcy1	A	G	11: 7,160,317	N855D	probably damaging	Het
Adgrl3	T	A	5: 81,774,404	C1199S	probably benign	Het
Alyref	G	A	11: 120,596,016	R140C	probably benign	Het
Arhgap26	A	T	18: 39,245,329	I92F	probably damaging	Het
Atp9b	G	A	18: 80,917,778	R73*	probably null	Het
Btaf1	G	A	19: 37,000,230	A1483T	probably damaging	Het
Casc1	A	G	6: 145,177,449	F564L	probably benign	Het
Cmtr2	T	C	8: 110,222,129	L357P	probably damaging	Het
Commd7	A	T	2: 153,628,554	L51Q	possibly damaging	Het
Cpd	T	A	11: 76,784,426	I1290L	probably benign	Het
Cry2	C	A	2: 92,413,648	E393D	probably damaging	Het
Ctsm	A	T	13: 61,537,829	M74K		Het
Dync2h1	T	C	9: 7,031,771	Y3491C	probably damaging	Het
Erc1	A	C	6: 119,824,881	N58K	possibly damaging	Het
Fbxw21	T	C	9: 109,146,696	T211A	probably benign	Het
Fry	T	C	5: 150,413,328	V1388A	probably benign	Het
Fuk	A	G	8: 110,883,925	W949R	probably damaging	Het
Gca	T	G	2: 62,690,024	I176S	probably damaging	Het
Gimap7	A	T	6: 48,723,827	K116*	probably null	Het
Gm40460	ACAACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	ACAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 142,240,710		probably benign	Het
Gm5415	G	T	1: 32,546,084	H248Q	probably benign	Het
Gm906	A	G	13: 50,247,381	F303S	probably benign	Het
Gm9195	A	C	14: 72,473,714	L428R	probably damaging	Het
Grin2b	A	G	6: 135,779,257	I490T	possibly damaging	Het
Kif24	T	C	4: 41,400,442	T499A	probably benign	Het
Mbtps1	G	A	8: 119,533,369	T413I	probably damaging	Het
Med4	A	T	14: 73,513,925	S105C	probably benign	Het
Mgat1	G	A	11: 49,261,083	R131Q	probably damaging	Het
Mybpc1	T	C	10: 88,543,753	E628G	possibly damaging	Het
Myh8	C	T	11: 67,292,434	P713L	possibly damaging	Het
Myo7a	T	C	7: 98,083,162	D706G	probably benign	Het
Nbas	T	A	12: 13,374,750	C997S	possibly damaging	Het
Nkx2-1	C	A	12: 56,534,967	G32C	probably damaging	Het
Npas3	A	G	12: 54,065,870	T466A	probably benign	Het
Olfr1085	T	A	2: 86,657,535	I308L	probably benign	Het
Olfr487	T	C	7: 108,211,962	E189G	probably benign	Het
Opa1	C	T	16: 29,620,414	R683C	probably benign	Het
Opa3	C	T	7: 19,255,541	R110C	probably damaging	Het
Ppfibp2	T	A	7: 107,738,318	L609*	probably null	Het
Pygo2	T	A	3: 89,433,310	D338E	possibly damaging	Het
Pzp	A	T	6: 128,525,209	M59K	probably benign	Het
Reln	A	G	5: 21,950,817		probably null	Het
Ripor1	A	G	8: 105,621,201	D1097G	unknown	Het
Rmdn3	T	C	2: 119,138,382	K443E	probably benign	Het
Rnf145	A	G	11: 44,557,435	D373G	possibly damaging	Het
Sec23b	A	T	2: 144,559,259	E13D	probably benign	Het
Slc39a6	A	G	18: 24,582,342	F707S	probably damaging	Het
Sox11	G	A	12: 27,341,537	A291V	possibly damaging	Het
Spg20	T	A	3: 55,124,846	V367D	possibly damaging	Het
Strap	A	T	6: 137,741,367	K156N	probably benign	Het
Stxbp5	T	A	10: 9,769,408	I951F	possibly damaging	Het
Taar7a	T	C	10: 23,992,779	I235V	probably benign	Het
Tek	T	A	4: 94,804,346	N230K	probably benign	Het
Tyw1	T	A	5: 130,296,679	C469*	probably null	Het
Vcan	T	C	13: 89,679,931	H3132R	probably damaging	Het
Vmn2r24	A	G	6: 123,806,473	D544G	probably damaging	Het
Vmn2r3	A	T	3: 64,278,982	M94K	possibly damaging	Het
Vps33a	T	C	5: 123,536,541	T388A	probably benign	Het
Vta1	A	G	10: 14,675,999	I152T	probably damaging	Het
Vtcn1	A	G	3: 100,892,549	D242G	probably benign	Het
Zfp12	T	A	5: 143,245,465	C548S	probably damaging	Het
Zfp263	A	G	16: 3,749,459	D546G	probably benign	Het
Zfp324	T	A	7: 12,970,762	C293S	probably damaging	Het
Zscan4c	A	T	7: 11,009,892	I473F	possibly damaging	Het

Other mutations in Mroh4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01645:Mroh4	APN	15	74611358	splice site	probably benign
IGL02370:Mroh4	APN	15	74625541	missense	probably benign	0.00
IGL02598:Mroh4	APN	15	74611243	critical splice donor site	probably null
IGL02644:Mroh4	APN	15	74610375	missense	possibly damaging	0.90
IGL02666:Mroh4	APN	15	74609775	missense	probably benign	0.04
IGL02723:Mroh4	APN	15	74608237	splice site	probably benign
IGL02724:Mroh4	APN	15	74606151	missense	probably benign	0.00
IGL03000:Mroh4	APN	15	74616114	missense	probably benign
IGL03103:Mroh4	APN	15	74616159	missense	possibly damaging	0.47
IGL03194:Mroh4	APN	15	74611539	missense	probably damaging	1.00
R0013:Mroh4	UTSW	15	74608237	splice site	probably benign
R0042:Mroh4	UTSW	15	74610305	missense	probably damaging	0.99
R0042:Mroh4	UTSW	15	74610305	missense	probably damaging	0.99
R0294:Mroh4	UTSW	15	74606149	missense	probably benign
R0346:Mroh4	UTSW	15	74614292	splice site	probably benign
R0545:Mroh4	UTSW	15	74625427	missense	probably benign	0.00
R0688:Mroh4	UTSW	15	74606678	missense	probably damaging	0.98
R1838:Mroh4	UTSW	15	74616113	missense	probably benign	0.03
R2037:Mroh4	UTSW	15	74609761	missense	possibly damaging	0.91
R4725:Mroh4	UTSW	15	74616107	missense	probably damaging	0.99
R4786:Mroh4	UTSW	15	74610234	missense	probably benign	0.08
R4798:Mroh4	UTSW	15	74626179	missense	probably damaging	1.00
R4945:Mroh4	UTSW	15	74612008	missense	probably benign	0.00
R5065:Mroh4	UTSW	15	74628270	splice site	probably null
R5476:Mroh4	UTSW	15	74611661	missense	probably benign	0.15
R5509:Mroh4	UTSW	15	74606154	missense	probably benign	0.00
R5527:Mroh4	UTSW	15	74615016	missense	probably damaging	1.00
R5662:Mroh4	UTSW	15	74625428	missense	possibly damaging	0.63
R5818:Mroh4	UTSW	15	74611982	missense	probably damaging	0.98
R5861:Mroh4	UTSW	15	74606607	intron	probably benign
R5886:Mroh4	UTSW	15	74606447	missense	possibly damaging	0.90
R5935:Mroh4	UTSW	15	74621154	missense	probably damaging	1.00
R6008:Mroh4	UTSW	15	74625472	nonsense	probably null
R6658:Mroh4	UTSW	15	74621129	missense	possibly damaging	0.83
R6689:Mroh4	UTSW	15	74612003	missense	probably damaging	1.00
R6739:Mroh4	UTSW	15	74609719	missense	probably benign	0.10
R6888:Mroh4	UTSW	15	74613249	missense	possibly damaging	0.93
R7088:Mroh4	UTSW	15	74626144	missense	probably benign	0.25
R7260:Mroh4	UTSW	15	74608129	missense	possibly damaging	0.83
R7365:Mroh4	UTSW	15	74610371	nonsense	probably null
R7735:Mroh4	UTSW	15	74625508	missense	probably damaging	0.98
R7763:Mroh4	UTSW	15	74624705	missense	probably damaging	0.99
R7945:Mroh4	UTSW	15	74624705	missense	probably damaging	0.99
R8090:Mroh4	UTSW	15	74624701	missense	probably benign	0.41
R8242:Mroh4	UTSW	15	74616308	missense	possibly damaging	0.47
R8978:Mroh4	UTSW	15	74627624	missense	probably benign	0.00
R9004:Mroh4	UTSW	15	74614322	missense	possibly damaging	0.65
R9083:Mroh4	UTSW	15	74626291	missense	probably damaging	1.00
R9248:Mroh4	UTSW	15	74613318	missense	possibly damaging	0.59
R9320:Mroh4	UTSW	15	74611556	missense	probably damaging	1.00
R9356:Mroh4	UTSW	15	74610911	missense	probably benign	0.05
R9512:Mroh4	UTSW	15	74613246	missense	probably benign	0.18
Z1177:Mroh4	UTSW	15	74627720	missense	probably damaging	1.00
Z1177:Mroh4	UTSW	15	74628002	missense	possibly damaging	0.83

Predicted Primers

PCR Primer
(F):5'- AACTTCTGGAGCTCTGAGTCTTG -3'
(R):5'- CACTTGCAGGAGCTTTCTGTG -3'

Sequencing Primer
(F):5'- AGCTCTGAGTCTTGAGCCC -3'
(R):5'- GGGGGCTTACTTTTCCCTCCAG -3'

Posted On

2022-02-07