Incidental Mutation 'R9182:Fstl4'
ID 697128
Institutional Source Beutler Lab
Gene Symbol Fstl4
Ensembl Gene ENSMUSG00000036264
Gene Name follistatin-like 4
Synonyms SPIG1, B230374F23Rik
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.151) question?
Stock # R9182 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 52655461-53079365 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 53024905 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Valine to Alanine at position 259 (V259A)
Ref Sequence ENSEMBL: ENSMUSP00000042007 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000036796]
AlphaFold Q5STE3
Predicted Effect probably damaging
Transcript: ENSMUST00000036796
AA Change: V259A

PolyPhen 2 Score 0.980 (Sensitivity: 0.75; Specificity: 0.96)
SMART Domains Protein: ENSMUSP00000042007
Gene: ENSMUSG00000036264
AA Change: V259A

DomainStartEndE-ValueType
signal peptide 1 22 N/A INTRINSIC
KAZAL 87 132 4.7e-13 SMART
Blast:IG_like 215 241 6e-7 BLAST
IGc2 260 327 1.9e-6 SMART
IGc2 352 419 1e-14 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.5%
Validation Efficiency 100% (99/99)
MGI Phenotype PHENOTYPE: Homozygous null mice were born at expected Mendelian ratio and healthy, fertile, apparently normal with normal retinal laminar structure. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 102 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2900026A02Rik A T 5: 113,243,473 (GRCm39) M190K probably damaging Het
4931406B18Rik A G 7: 43,153,640 (GRCm39) W3R probably damaging Het
Acap3 T C 4: 155,989,892 (GRCm39) L720P probably damaging Het
Actc1 T C 2: 113,882,494 (GRCm39) T7A probably benign Het
Angpt2 T A 8: 18,760,658 (GRCm39) probably null Het
Babam2 T A 5: 32,058,401 (GRCm39) D228E possibly damaging Het
Ccar1 G A 10: 62,621,347 (GRCm39) L34F probably damaging Het
Ccdc83 T C 7: 89,886,102 (GRCm39) D219G probably damaging Het
Cct2 A G 10: 116,892,025 (GRCm39) V341A probably benign Het
Cdc20b G A 13: 113,208,503 (GRCm39) probably null Het
Chd7 T A 4: 8,838,737 (GRCm39) L1312Q probably damaging Het
Cltc G A 11: 86,595,982 (GRCm39) T1252I probably damaging Het
Cnpy1 G T 5: 28,450,799 (GRCm39) D2E possibly damaging Het
Crat C T 2: 30,298,085 (GRCm39) V205M probably damaging Het
Cspg4b A G 13: 113,457,358 (GRCm39) N1135D Het
Dgkb T C 12: 38,216,776 (GRCm39) probably null Het
Dglucy T C 12: 100,811,028 (GRCm39) V254A possibly damaging Het
Disp3 A G 4: 148,354,841 (GRCm39) S392P probably damaging Het
Dlx6 A T 6: 6,863,456 (GRCm39) Q26L probably benign Het
Dnah3 T C 7: 119,684,351 (GRCm39) D258G probably damaging Het
Dnah6 A T 6: 73,121,688 (GRCm39) Y1431* probably null Het
Dnah7b T C 1: 46,330,038 (GRCm39) I3294T probably benign Het
Dnai2 A G 11: 114,623,839 (GRCm39) N34S probably benign Het
Dock2 T C 11: 34,260,398 (GRCm39) K981R possibly damaging Het
Entpd8 T C 2: 24,971,931 (GRCm39) I33T probably benign Het
Eppk1 A G 15: 75,995,453 (GRCm39) L476P probably benign Het
Etv1 T A 12: 38,830,716 (GRCm39) probably null Het
Fads2b T C 2: 85,330,581 (GRCm39) H242R probably benign Het
Fam193a T A 5: 34,623,361 (GRCm39) N1316K possibly damaging Het
Fkbp4 T C 6: 128,415,382 (GRCm39) E31G probably benign Het
Fnbp1l C T 3: 122,363,345 (GRCm39) D81N probably damaging Het
Glipr1l1 T C 10: 111,912,056 (GRCm39) S197P probably damaging Het
Gprin3 A T 6: 59,331,197 (GRCm39) M370K probably benign Het
Gsc G T 12: 104,439,120 (GRCm39) Y85* probably null Het
Gys2 T G 6: 142,391,735 (GRCm39) Y509S probably damaging Het
Gys2 T C 6: 142,406,978 (GRCm39) N126S possibly damaging Het
Hmcn1 A T 1: 150,488,405 (GRCm39) W4292R probably damaging Het
Hmcn1 C A 1: 150,500,337 (GRCm39) E3952* probably null Het
Hsd3b9 C T 3: 98,354,005 (GRCm39) A165T possibly damaging Het
Htr1f T C 16: 64,746,825 (GRCm39) M156V probably benign Het
Il21r G A 7: 125,228,213 (GRCm39) G228E probably benign Het
Kat6b C T 14: 21,567,187 (GRCm39) S82L probably benign Het
Kntc1 T A 5: 123,940,355 (GRCm39) L1651Q probably damaging Het
Lipi G T 16: 75,357,673 (GRCm39) Y297* probably null Het
Mapre3 T C 5: 31,022,016 (GRCm39) S190P possibly damaging Het
Mmp8 T A 9: 7,567,467 (GRCm39) Y442* probably null Het
Mmut A G 17: 41,252,310 (GRCm39) E284G probably damaging Het
Mt3 G A 8: 94,880,615 (GRCm39) E62K unknown Het
Myh13 A G 11: 67,228,579 (GRCm39) E373G probably damaging Het
Myh7 T C 14: 55,226,374 (GRCm39) Y422C probably damaging Het
Nalcn A G 14: 123,834,016 (GRCm39) V53A probably damaging Het
Nucb2 A G 7: 116,121,070 (GRCm39) probably null Het
Opn5 A T 17: 42,903,890 (GRCm39) H148Q probably damaging Het
Or13a23-ps1 A T 7: 140,118,782 (GRCm39) L117F probably benign Het
Or51h1 A T 7: 102,308,927 (GRCm39) I300L probably damaging Het
Or51v14 T C 7: 103,261,063 (GRCm39) K166E possibly damaging Het
Or6b1 A T 6: 42,815,010 (GRCm39) N65I probably benign Het
Or7g16 G T 9: 18,726,742 (GRCm39) Q283K probably damaging Het
Or8b56 C A 9: 38,739,172 (GRCm39) H56N probably damaging Het
Osmr T A 15: 6,850,569 (GRCm39) D679V probably damaging Het
Pde4dip T C 3: 97,602,314 (GRCm39) D2305G probably benign Het
Pdgfrl A T 8: 41,429,996 (GRCm39) Q121L probably damaging Het
Pls1 C A 9: 95,658,811 (GRCm39) V226F probably damaging Het
Plxnd1 T A 6: 115,970,746 (GRCm39) I341F probably damaging Het
Ppfia2 T A 10: 106,763,640 (GRCm39) M1211K probably benign Het
Ppfibp2 T A 7: 107,308,053 (GRCm39) Y229N possibly damaging Het
Prss53 A G 7: 127,487,554 (GRCm39) S266P probably benign Het
Pycr2 C T 1: 180,734,397 (GRCm39) Q226* probably null Het
Rad50 T C 11: 53,583,590 (GRCm39) H397R probably benign Het
Raf1 T C 6: 115,600,440 (GRCm39) E393G probably damaging Het
Reln T C 5: 22,106,617 (GRCm39) D3251G probably benign Het
Robo4 A T 9: 37,313,206 (GRCm39) probably benign Het
Sbf1 A T 15: 89,173,806 (GRCm39) probably null Het
Serpinb6a T C 13: 34,109,360 (GRCm39) D145G probably damaging Het
Setx T A 2: 29,061,299 (GRCm39) M2236K probably damaging Het
Sgms1 A G 19: 32,101,758 (GRCm39) V416A probably benign Het
Six5 T G 7: 18,830,932 (GRCm39) S520A probably benign Het
Sox5 T A 6: 143,779,118 (GRCm39) I722F possibly damaging Het
Spag17 T G 3: 99,966,158 (GRCm39) I1147S possibly damaging Het
Spice1 C T 16: 44,206,065 (GRCm39) T842I possibly damaging Het
Ssbp2 A G 13: 91,848,800 (GRCm39) T358A probably damaging Het
Taok3 T C 5: 117,355,307 (GRCm39) probably null Het
Tbc1d2b C A 9: 90,152,652 (GRCm39) C38F probably damaging Het
Tecrl C A 5: 83,457,049 (GRCm39) probably null Het
Tgm7 T C 2: 120,926,980 (GRCm39) N390D probably benign Het
Thsd4 T G 9: 59,894,649 (GRCm39) M770L probably benign Het
Tmod2 C A 9: 75,504,624 (GRCm39) probably benign Het
Tnks A G 8: 35,308,905 (GRCm39) probably null Het
Tox3 T C 8: 90,984,507 (GRCm39) N224S probably benign Het
Trappc10 C T 10: 78,050,464 (GRCm39) E326K probably damaging Het
Usp16 T A 16: 87,276,542 (GRCm39) F627I probably benign Het
Vcam1 T C 3: 115,911,004 (GRCm39) I465V probably benign Het
Vmn1r128 T C 7: 21,083,683 (GRCm39) I129T possibly damaging Het
Vmn1r217 T A 13: 23,298,495 (GRCm39) I136L probably benign Het
Vmn2r2 A T 3: 64,044,802 (GRCm39) C115S probably damaging Het
Vmn2r85 A C 10: 130,265,350 (GRCm39) S44R probably benign Het
Wasf3 T A 5: 146,403,635 (GRCm39) Y248* probably null Het
Yipf5 A T 18: 40,343,851 (GRCm39) L127M probably damaging Het
Zbp1 G T 2: 173,050,933 (GRCm39) C312* probably null Het
Zfp423 A T 8: 88,508,742 (GRCm39) F534Y probably damaging Het
Zfp69 T G 4: 120,792,308 (GRCm39) Y156S probably damaging Het
Zmat4 A G 8: 24,419,226 (GRCm39) N155S probably damaging Het
Other mutations in Fstl4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00093:Fstl4 APN 11 53,077,102 (GRCm39) missense probably benign 0.01
IGL00885:Fstl4 APN 11 53,039,809 (GRCm39) missense possibly damaging 0.90
IGL00915:Fstl4 APN 11 53,067,825 (GRCm39) missense probably benign
IGL00933:Fstl4 APN 11 53,077,588 (GRCm39) missense possibly damaging 0.80
IGL01022:Fstl4 APN 11 53,077,568 (GRCm39) missense probably benign 0.18
IGL01121:Fstl4 APN 11 52,705,464 (GRCm39) missense probably benign 0.00
IGL01656:Fstl4 APN 11 52,891,201 (GRCm39) missense probably damaging 1.00
IGL01805:Fstl4 APN 11 53,077,184 (GRCm39) missense probably damaging 1.00
IGL01997:Fstl4 APN 11 53,053,881 (GRCm39) nonsense probably null
IGL02386:Fstl4 APN 11 52,664,698 (GRCm39) missense probably benign 0.21
IGL02536:Fstl4 APN 11 53,024,851 (GRCm39) splice site probably benign
IGL02807:Fstl4 APN 11 53,077,501 (GRCm39) missense probably benign 0.03
IGL03037:Fstl4 APN 11 53,059,050 (GRCm39) missense possibly damaging 0.83
R0462:Fstl4 UTSW 11 53,077,229 (GRCm39) missense probably benign 0.09
R1190:Fstl4 UTSW 11 52,959,373 (GRCm39) missense probably benign
R1300:Fstl4 UTSW 11 52,959,454 (GRCm39) missense probably benign
R1626:Fstl4 UTSW 11 52,891,117 (GRCm39) nonsense probably null
R1695:Fstl4 UTSW 11 53,056,705 (GRCm39) splice site probably null
R1699:Fstl4 UTSW 11 53,059,005 (GRCm39) missense possibly damaging 0.81
R1727:Fstl4 UTSW 11 52,959,478 (GRCm39) missense probably damaging 1.00
R1752:Fstl4 UTSW 11 53,077,622 (GRCm39) missense probably benign 0.09
R1866:Fstl4 UTSW 11 53,077,225 (GRCm39) missense probably benign 0.00
R4689:Fstl4 UTSW 11 52,959,477 (GRCm39) nonsense probably null
R5126:Fstl4 UTSW 11 53,077,388 (GRCm39) missense possibly damaging 0.71
R5129:Fstl4 UTSW 11 53,077,266 (GRCm39) missense probably damaging 1.00
R5499:Fstl4 UTSW 11 52,959,374 (GRCm39) missense probably benign 0.01
R5578:Fstl4 UTSW 11 53,056,608 (GRCm39) missense probably damaging 1.00
R5715:Fstl4 UTSW 11 52,891,243 (GRCm39) missense possibly damaging 0.53
R6125:Fstl4 UTSW 11 53,077,130 (GRCm39) missense probably benign
R6177:Fstl4 UTSW 11 53,059,031 (GRCm39) missense probably benign 0.00
R6236:Fstl4 UTSW 11 53,077,162 (GRCm39) missense probably benign 0.00
R6311:Fstl4 UTSW 11 53,067,804 (GRCm39) missense probably damaging 1.00
R6611:Fstl4 UTSW 11 53,077,552 (GRCm39) missense probably benign 0.01
R6886:Fstl4 UTSW 11 53,077,277 (GRCm39) missense probably damaging 1.00
R7404:Fstl4 UTSW 11 53,024,898 (GRCm39) missense probably benign 0.03
R7423:Fstl4 UTSW 11 52,959,382 (GRCm39) missense possibly damaging 0.54
R7586:Fstl4 UTSW 11 52,963,256 (GRCm39) missense probably benign 0.00
R7756:Fstl4 UTSW 11 53,059,123 (GRCm39) missense possibly damaging 0.69
R7758:Fstl4 UTSW 11 53,059,123 (GRCm39) missense possibly damaging 0.69
R7775:Fstl4 UTSW 11 53,067,798 (GRCm39) nonsense probably null
R7953:Fstl4 UTSW 11 52,891,050 (GRCm39) missense probably benign 0.01
R8026:Fstl4 UTSW 11 52,959,496 (GRCm39) missense probably damaging 0.99
R8043:Fstl4 UTSW 11 52,891,050 (GRCm39) missense probably benign 0.01
R8375:Fstl4 UTSW 11 53,053,502 (GRCm39) missense possibly damaging 0.63
R8866:Fstl4 UTSW 11 52,963,233 (GRCm39) missense possibly damaging 0.54
R9103:Fstl4 UTSW 11 52,664,696 (GRCm39) missense probably benign 0.21
R9297:Fstl4 UTSW 11 53,024,973 (GRCm39) missense possibly damaging 0.50
R9390:Fstl4 UTSW 11 52,891,102 (GRCm39) missense probably benign
R9396:Fstl4 UTSW 11 52,664,778 (GRCm39) missense probably benign
R9447:Fstl4 UTSW 11 53,077,166 (GRCm39) missense probably damaging 1.00
R9506:Fstl4 UTSW 11 53,024,950 (GRCm39) missense probably benign 0.18
R9518:Fstl4 UTSW 11 53,056,647 (GRCm39) missense possibly damaging 0.71
R9523:Fstl4 UTSW 11 53,075,466 (GRCm39) missense probably benign 0.04
R9586:Fstl4 UTSW 11 53,077,729 (GRCm39) missense probably benign 0.02
R9594:Fstl4 UTSW 11 52,664,694 (GRCm39) missense probably benign
X0013:Fstl4 UTSW 11 53,053,446 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ACCCAGTGAGGATGTCTATCAG -3'
(R):5'- TCTGCCACTACTGCCACATG -3'

Sequencing Primer
(F):5'- ACTGTCACATTATAACCCATTTCTGG -3'
(R):5'- ACATGGTGCCCCACAAGTTG -3'
Posted On 2022-02-07