Mutagenetix > Incidental Mutation

Incidental Mutation 'R9183:Zfp160'

697213

Institutional Source

Beutler Lab

Gene Symbol

Zfp160

Ensembl Gene

ENSMUSG00000067942

Gene Name

zinc finger protein 160

Synonyms

6720480D16Rik, 6720480D16Rik

MMRRC Submission

068950-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9183 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

21229203-21249119 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 21240354 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 12 (D12E)

Ref Sequence

ENSEMBL: ENSMUSP00000086191 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088811] [ENSMUST00000231482] [ENSMUST00000232320] [ENSMUST00000232354] [ENSMUST00000232473] [ENSMUST00000232595] [ENSMUST00000232663]

AlphaFold

E9Q459

Predicted Effect

possibly damaging
Transcript: ENSMUST00000088811
AA Change: D12E

PolyPhen 2 Score 0.837 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000086191
Gene: ENSMUSG00000067942
AA Change: D12E

Domain	Start	End	E-Value	Type
KRAB	8	68	1.91e-29	SMART
low complexity region	100	110	N/A	INTRINSIC
ZnF_C2H2	146	168	1.69e-3	SMART
ZnF_C2H2	174	196	2.91e-2	SMART
ZnF_C2H2	202	224	1.4e-4	SMART
ZnF_C2H2	230	252	3.89e-3	SMART
ZnF_C2H2	258	280	1.72e-4	SMART
ZnF_C2H2	286	308	4.94e-5	SMART
ZnF_C2H2	314	336	2.12e-4	SMART
ZnF_C2H2	342	364	1.12e-3	SMART
ZnF_C2H2	370	392	1.2e-3	SMART
ZnF_C2H2	398	420	6.42e-4	SMART
ZnF_C2H2	426	448	9.08e-4	SMART
ZnF_C2H2	454	476	1.84e-4	SMART
ZnF_C2H2	482	504	1.5e-4	SMART
ZnF_C2H2	510	532	3.44e-4	SMART
ZnF_C2H2	538	560	1.12e-3	SMART
ZnF_C2H2	566	588	2.27e-4	SMART
ZnF_C2H2	594	616	1.04e-3	SMART
ZnF_C2H2	622	644	1.13e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000231482

Predicted Effect

probably damaging
Transcript: ENSMUST00000232320
AA Change: D12E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000232354
AA Change: D12E

PolyPhen 2 Score 0.837 (Sensitivity: 0.84; Specificity: 0.93)

Predicted Effect

probably benign
Transcript: ENSMUST00000232473

Predicted Effect

probably damaging
Transcript: ENSMUST00000232595
AA Change: D12E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000232663
AA Change: D12E

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox2	A	G	14: 8,251,559 (GRCm38)	C313R	probably damaging	Het
Adap2	A	G	11: 80,045,882 (GRCm39)	N54S	probably damaging	Het
Adcy8	T	C	15: 64,694,116 (GRCm39)	D387G	probably damaging	Het
Adgra1	G	A	7: 139,455,716 (GRCm39)	R448Q	probably benign	Het
Antxr1	A	T	6: 87,264,025 (GRCm39)	D148E	probably damaging	Het
Ap3d1	A	T	10: 80,545,627 (GRCm39)	V1034E	probably null	Het
Arpp21	C	T	9: 111,895,066 (GRCm39)	A731T	probably benign	Het
Asl	T	A	5: 130,042,312 (GRCm39)	R255W	probably damaging	Het
Asxl1	T	A	2: 153,239,840 (GRCm39)	S544T	probably damaging	Het
Atp8a1	A	C	5: 67,924,378 (GRCm39)	Y329D		Het
Calcr	A	T	6: 3,711,463 (GRCm39)	I186N	probably damaging	Het
Cdh18	T	C	15: 23,227,065 (GRCm39)		probably null	Het
Celsr3	T	C	9: 108,706,595 (GRCm39)	L1026P	probably damaging	Het
Ces2a	T	A	8: 105,460,774 (GRCm39)	V24E	possibly damaging	Het
Clybl	T	C	14: 122,639,387 (GRCm39)	I317T	probably damaging	Het
Col13a1	T	A	10: 61,699,758 (GRCm39)	T476S	unknown	Het
Cyp4f15	T	C	17: 32,919,205 (GRCm39)	S343P	probably damaging	Het
D630003M21Rik	T	C	2: 158,059,112 (GRCm39)	T263A	probably benign	Het
Fbxo28	A	G	1: 182,157,526 (GRCm39)	V97A	possibly damaging	Het
Frem2	C	T	3: 53,427,486 (GRCm39)	V2889I	probably damaging	Het
Garnl3	T	C	2: 32,895,080 (GRCm39)	E663G	probably damaging	Het
Gen1	T	A	12: 11,299,186 (GRCm39)	K338M	probably damaging	Het
Gfi1	A	G	5: 107,873,819 (GRCm39)		probably null	Het
Gfod2	A	G	8: 106,449,653 (GRCm39)	S95P	probably benign	Het
H2-T13	T	A	17: 36,392,382 (GRCm39)	H31L	unknown	Het
Heatr1	A	G	13: 12,436,266 (GRCm39)	Q1224R	probably damaging	Het
Hectd4	A	G	5: 121,437,551 (GRCm39)	K1059E	possibly damaging	Het
Hmgcs2	C	T	3: 98,198,232 (GRCm39)	A45V	possibly damaging	Het
Hnmt	C	A	2: 23,893,655 (GRCm39)	V280L	probably benign	Het
Ifi35	G	T	11: 101,348,091 (GRCm39)	V66L	probably benign	Het
Kcnn2	T	C	18: 45,694,379 (GRCm39)	I188T	probably damaging	Het
Klhl8	C	A	5: 104,012,111 (GRCm39)	A575S	probably benign	Het
Krt6a	T	C	15: 101,601,446 (GRCm39)	D225G	probably benign	Het
Lrtm2	T	C	6: 119,294,384 (GRCm39)	Y249C	probably damaging	Het
Med12l	C	T	3: 58,984,498 (GRCm39)	R479C	probably damaging	Het
Muc5ac	A	T	7: 141,352,637 (GRCm39)	Y709F	possibly damaging	Het
Nsd2	G	A	5: 34,028,796 (GRCm39)	A162T	probably damaging	Het
Or6c38	A	G	10: 128,929,201 (GRCm39)	M214T	probably benign	Het
Phaf1	C	A	8: 105,957,840 (GRCm39)	Q49K	probably benign	Het
Polr2h	T	C	16: 20,539,285 (GRCm39)	Y90H	possibly damaging	Het
Prkaa1	A	T	15: 5,205,969 (GRCm39)	E275V	probably damaging	Het
Prss22	C	T	17: 24,213,592 (GRCm39)	G233E	probably damaging	Het
Rhcg	A	T	7: 79,244,564 (GRCm39)	L496*	probably null	Het
Rnf213	A	G	11: 119,318,448 (GRCm39)	E1084G		Het
Safb2	ACTTCTTCT	ACTTCT	17: 56,878,292 (GRCm39)		probably benign	Het
Slc14a1	T	A	18: 78,154,598 (GRCm39)	I263F	probably benign	Het
Slc40a1	C	T	1: 45,948,671 (GRCm39)	M536I	possibly damaging	Het
Slco6c1	T	A	1: 96,996,775 (GRCm39)		probably null	Het
Stk32a	T	C	18: 43,394,405 (GRCm39)	F118S	probably damaging	Het
Sytl1	G	T	4: 132,980,934 (GRCm39)	R467S	possibly damaging	Het
Thbs2	T	C	17: 14,896,526 (GRCm39)	I788V	probably benign	Het
Tmed6	T	A	8: 107,788,390 (GRCm39)	R186*	probably null	Het
Trnau1ap	G	A	4: 132,052,565 (GRCm39)	R78C	probably damaging	Het
Trpv1	A	T	11: 73,135,039 (GRCm39)	D412V	possibly damaging	Het
Ttc16	C	T	2: 32,647,329 (GRCm39)	V688M	probably benign	Het
Ubr5	C	A	15: 37,997,420 (GRCm39)	L1738F		Het
Vmn2r89	A	G	14: 51,692,501 (GRCm39)	I101M	probably benign	Het
Wdr27	C	T	17: 15,148,651 (GRCm39)	R114Q	possibly damaging	Het
Wdr48	A	G	9: 119,749,730 (GRCm39)	H563R	possibly damaging	Het
Wdr49	A	C	3: 75,205,419 (GRCm39)	S666A	probably benign	Het
Wwox	T	C	8: 115,433,110 (GRCm39)	S259P	probably damaging	Het
Zfp800	A	G	6: 28,243,172 (GRCm39)	Y598H	probably benign	Het
Zfp970	T	C	2: 177,167,536 (GRCm39)	L370P	probably damaging	Het
Zmat5	A	T	11: 4,672,431 (GRCm39)	D16V	probably damaging	Het

Other mutations in Zfp160

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00714:Zfp160	APN	17	21,246,964 (GRCm39)	missense	probably benign
IGL01019:Zfp160	APN	17	21,241,088 (GRCm39)	missense	possibly damaging	0.68
IGL02430:Zfp160	APN	17	21,245,792 (GRCm39)	missense	possibly damaging	0.76
R0412:Zfp160	UTSW	17	21,247,139 (GRCm39)	missense	probably damaging	0.97
R0600:Zfp160	UTSW	17	21,247,268 (GRCm39)	missense	probably benign	0.00
R2146:Zfp160	UTSW	17	21,247,244 (GRCm39)	missense	probably benign	0.13
R2157:Zfp160	UTSW	17	21,241,090 (GRCm39)	missense	probably benign	0.23
R2411:Zfp160	UTSW	17	21,246,007 (GRCm39)	missense	possibly damaging	0.94
R2904:Zfp160	UTSW	17	21,245,911 (GRCm39)	missense	probably benign	0.00
R4249:Zfp160	UTSW	17	21,246,000 (GRCm39)	missense	probably benign	0.11
R4896:Zfp160	UTSW	17	21,240,343 (GRCm39)	missense	probably benign	0.00
R5106:Zfp160	UTSW	17	21,247,023 (GRCm39)	missense	probably damaging	0.99
R5342:Zfp160	UTSW	17	21,240,995 (GRCm39)	missense	possibly damaging	0.95
R5352:Zfp160	UTSW	17	21,247,114 (GRCm39)	missense	probably benign	0.02
R6193:Zfp160	UTSW	17	21,247,124 (GRCm39)	missense	probably benign	0.24
R6230:Zfp160	UTSW	17	21,246,707 (GRCm39)	missense	probably benign	0.38
R6753:Zfp160	UTSW	17	21,240,996 (GRCm39)	missense	probably benign	0.02
R6928:Zfp160	UTSW	17	21,261,724 (GRCm39)	missense	probably benign	0.04
R7040:Zfp160	UTSW	17	21,246,794 (GRCm39)	missense	probably damaging	1.00
R7255:Zfp160	UTSW	17	21,245,749 (GRCm39)	missense	probably benign	0.18
R7497:Zfp160	UTSW	17	21,246,455 (GRCm39)	missense	probably benign	0.08
R7510:Zfp160	UTSW	17	21,246,655 (GRCm39)	missense	probably benign	0.00
R7540:Zfp160	UTSW	17	21,245,922 (GRCm39)	nonsense	probably null
R7627:Zfp160	UTSW	17	21,247,270 (GRCm39)	missense	probably damaging	0.99
R8169:Zfp160	UTSW	17	21,247,298 (GRCm39)	missense	probably damaging	0.97
R8240:Zfp160	UTSW	17	21,246,350 (GRCm39)	missense	probably damaging	0.99
R8330:Zfp160	UTSW	17	21,246,313 (GRCm39)	missense	probably damaging	1.00
R8367:Zfp160	UTSW	17	21,245,804 (GRCm39)	missense	probably benign	0.22
R8802:Zfp160	UTSW	17	21,246,867 (GRCm39)	missense	probably damaging	1.00
R9556:Zfp160	UTSW	17	21,247,031 (GRCm39)	missense	probably benign	0.03
R9695:Zfp160	UTSW	17	21,245,746 (GRCm39)	missense	possibly damaging	0.53
Z1177:Zfp160	UTSW	17	21,247,152 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTTATCAGTCCAGAGATGACAAG -3'
(R):5'- GAAAAGTTAAATGGCTCCCCAAAG -3'

Sequencing Primer
(F):5'- GTCCAGAGATGACAAGTGAATTACAC -3'
(R):5'- TGGCTCCCCAAAGGCACAG -3'

Posted On

2022-02-07