Mutagenetix > Incidental Mutation

Incidental Mutation 'R9197:Zfp970'

697974

Institutional Source

Beutler Lab

Gene Symbol

Zfp970

Ensembl Gene

ENSMUSG00000078866

Gene Name

zinc finger protein 970

Synonyms

Gm14420

MMRRC Submission

068956-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.223) question?

Stock #

R9197 (G1)

Quality Score

180.009

Status

Not validated

Chromosome

Chromosomal Location

177156526-177169922 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 177167878 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 484 (I484T)

Ref Sequence

ENSEMBL: ENSMUSP00000104580 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099002] [ENSMUST00000108952]

AlphaFold

Q08BU3

Predicted Effect

probably benign
Transcript: ENSMUST00000099002

SMART Domains

Protein: ENSMUSP00000096600
Gene: ENSMUSG00000078866

Domain	Start	End	E-Value	Type
KRAB	4	66	3.68e-13	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108952
AA Change: I484T

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000104580
Gene: ENSMUSG00000078866
AA Change: I484T

Domain	Start	End	E-Value	Type
KRAB	4	66	1.13e-13	SMART
ZnF_C2H2	78	97	1.53e2	SMART
ZnF_C2H2	103	125	8.75e0	SMART
ZnF_C2H2	131	153	1.28e-3	SMART
ZnF_C2H2	159	181	3.89e-3	SMART
ZnF_C2H2	187	209	3.63e-3	SMART
ZnF_C2H2	215	237	3.89e-3	SMART
ZnF_C2H2	243	265	1.58e-3	SMART
ZnF_C2H2	271	293	7.9e-4	SMART
ZnF_C2H2	299	321	3.69e-4	SMART
ZnF_C2H2	327	349	2.12e-4	SMART
ZnF_C2H2	355	377	1.56e-2	SMART
ZnF_C2H2	383	405	1.12e-3	SMART
ZnF_C2H2	411	433	1.79e-2	SMART
ZnF_C2H2	439	461	3.69e-4	SMART
ZnF_C2H2	467	489	3.21e-4	SMART
ZnF_C2H2	495	517	7.9e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

100% (44/44)

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
BC048679	C	T	7: 81,144,966 (GRCm39)	V126M	probably benign	Het
Brd2	T	C	17: 34,333,370 (GRCm39)	Y427C	probably damaging	Het
Btbd1	T	A	7: 81,443,363 (GRCm39)	T474S	probably damaging	Het
Cacna1c	T	C	6: 118,590,950 (GRCm39)	T1524A		Het
Capn9	T	A	8: 125,340,600 (GRCm39)	V588D	probably damaging	Het
Ccdc88b	T	C	19: 6,833,213 (GRCm39)	E278G	probably damaging	Het
Ccr3	T	C	9: 123,829,732 (GRCm39)	S356P	probably damaging	Het
Cplane1	A	T	15: 8,280,536 (GRCm39)	I2820F	unknown	Het
Cyfip1	C	T	7: 55,554,222 (GRCm39)	H754Y	probably null	Het
Dolk	A	G	2: 30,174,693 (GRCm39)	S451P	probably damaging	Het
Elac2	G	A	11: 64,892,682 (GRCm39)	A829T	probably benign	Het
Fahd2a	T	C	2: 127,278,284 (GRCm39)	I308V	possibly damaging	Het
Gm7168	T	A	17: 14,169,489 (GRCm39)	N285K	probably benign	Het
Hgf	T	A	5: 16,766,059 (GRCm39)	V65E	probably benign	Het
Igsf21	A	T	4: 139,762,084 (GRCm39)	L198Q	probably benign	Het
Il18rap	C	T	1: 40,582,177 (GRCm39)	T366M	probably benign	Het
Ipo4	C	T	14: 55,870,840 (GRCm39)	R264H	probably damaging	Het
Jak2	C	T	19: 29,289,157 (GRCm39)	T1103I	probably benign	Het
Kdm4d	T	A	9: 14,375,537 (GRCm39)	Y107F	probably damaging	Het
Lad1	T	A	1: 135,759,630 (GRCm39)	S509T	probably benign	Het
Lhx5	C	A	5: 120,573,446 (GRCm39)	P143Q	possibly damaging	Het
Lilra6	T	C	7: 3,915,716 (GRCm39)	T340A	possibly damaging	Het
Lrrc4	T	C	6: 28,831,318 (GRCm39)	H99R	probably benign	Het
Mmp11	T	A	10: 75,763,067 (GRCm39)	D175V	probably damaging	Het
Msh2	T	A	17: 88,026,943 (GRCm39)	M813K	possibly damaging	Het
Mvp	T	C	7: 126,588,959 (GRCm39)	N603D	probably damaging	Het
Or5k3	T	A	16: 58,969,489 (GRCm39)	I92N	probably damaging	Het
Or8c18	G	A	9: 38,204,080 (GRCm39)	V280M	possibly damaging	Het
Pcdha2	A	C	18: 37,072,879 (GRCm39)	Y170S	probably damaging	Het
Pcdhb19	G	A	18: 37,631,354 (GRCm39)	C383Y	probably damaging	Het
Pcdhga2	A	T	18: 37,804,553 (GRCm39)	D799V	probably benign	Het
Pias3	A	G	3: 96,611,064 (GRCm39)	Y430C	probably benign	Het
Pign	A	G	1: 105,516,818 (GRCm39)	F575L	probably benign	Het
Rin3	A	T	12: 102,335,306 (GRCm39)	I406F	probably benign	Het
Rpgrip1	A	T	14: 52,382,857 (GRCm39)	E674D	possibly damaging	Het
Snx13	T	C	12: 35,155,196 (GRCm39)	V420A	probably benign	Het
Spata31d1c	A	T	13: 65,183,690 (GRCm39)	M411L	probably benign	Het
Spata31h1	C	A	10: 82,120,401 (GRCm39)	C4203F	possibly damaging	Het
Tmem150a	T	G	6: 72,333,722 (GRCm39)	M28R	probably benign	Het
Trp53	T	C	11: 69,480,000 (GRCm39)	Y233H	probably damaging	Het
Ufl1	A	G	4: 25,250,519 (GRCm39)	I778T	possibly damaging	Het
Vmn2r89	T	A	14: 51,693,596 (GRCm39)	H315Q	possibly damaging	Het
Wapl	T	A	14: 34,444,244 (GRCm39)	N565K	probably damaging	Het
Zfhx2	G	A	14: 55,312,179 (GRCm39)	Q172*	probably null	Het
Zfp592	T	A	7: 80,674,067 (GRCm39)	S344T	possibly damaging	Het

Other mutations in Zfp970

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02900:Zfp970	APN	2	177,166,610 (GRCm39)	splice site	probably benign
R1817:Zfp970	UTSW	2	177,167,976 (GRCm39)	missense	probably damaging	1.00
R1992:Zfp970	UTSW	2	177,166,663 (GRCm39)	missense	possibly damaging	0.55
R2252:Zfp970	UTSW	2	177,166,614 (GRCm39)	splice site	probably null
R2253:Zfp970	UTSW	2	177,166,614 (GRCm39)	splice site	probably null
R4576:Zfp970	UTSW	2	177,167,473 (GRCm39)	missense	probably damaging	0.99
R4617:Zfp970	UTSW	2	177,167,961 (GRCm39)	missense	probably benign	0.16
R4828:Zfp970	UTSW	2	177,167,146 (GRCm39)	missense	probably damaging	0.98
R5801:Zfp970	UTSW	2	177,165,151 (GRCm39)	missense	probably damaging	1.00
R6198:Zfp970	UTSW	2	177,167,253 (GRCm39)	missense	probably damaging	1.00
R6540:Zfp970	UTSW	2	177,167,388 (GRCm39)	missense	probably damaging	1.00
R6793:Zfp970	UTSW	2	177,167,338 (GRCm39)	missense	probably damaging	1.00
R7092:Zfp970	UTSW	2	177,167,085 (GRCm39)	missense	probably damaging	1.00
R7289:Zfp970	UTSW	2	177,167,086 (GRCm39)	missense	probably damaging	1.00
R7830:Zfp970	UTSW	2	177,167,338 (GRCm39)	missense	probably damaging	1.00
R8854:Zfp970	UTSW	2	177,165,088 (GRCm39)	missense	probably damaging	0.98
R9003:Zfp970	UTSW	2	177,167,010 (GRCm39)	missense	probably damaging	1.00
R9183:Zfp970	UTSW	2	177,167,536 (GRCm39)	missense	probably damaging	1.00
R9446:Zfp970	UTSW	2	177,167,064 (GRCm39)	nonsense	probably null
R9625:Zfp970	UTSW	2	177,167,790 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TCTCCAAGGACATGAGCGA -3'
(R):5'- TGTATTTGAAGGTCACCACTTCTT -3'

Sequencing Primer
(F):5'- GTACAATGCATTGCTCTCCGAATAC -3'
(R):5'- CACATTGCTTACATTCACAGGG -3'

Posted On

2022-02-07