Mutagenetix > Incidental Mutation

Incidental Mutation 'R9209:Crb1'

698678

Institutional Source

Beutler Lab

Gene Symbol

Crb1

Ensembl Gene

ENSMUSG00000063681

Gene Name

crumbs family member 1, photoreceptor morphogenesis associated

Synonyms

A930008G09Rik, 7530426H14Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.303) question?

Stock #

R9209 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

139124794-139304838 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 139171051 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 780 (K780E)

Ref Sequence

ENSEMBL: ENSMUSP00000060769 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059825] [ENSMUST00000196402] [ENSMUST00000198445]

AlphaFold

Q8VHS2

Predicted Effect

probably damaging
Transcript: ENSMUST00000059825
AA Change: K780E

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000060769
Gene: ENSMUSG00000063681
AA Change: K780E

Domain	Start	End	E-Value	Type
low complexity region	8	19	N/A	INTRINSIC
EGF	72	107	5.97e-4	SMART
EGF	112	145	9.19e-5	SMART
EGF_CA	147	183	2.89e-11	SMART
EGF_CA	185	221	1.14e-9	SMART
EGF_CA	223	259	2.26e-13	SMART
EGF_CA	261	298	5.15e-8	SMART
EGF	303	336	8.12e-6	SMART
EGF	341	394	2.6e-4	SMART
EGF_CA	396	438	2.54e-7	SMART
EGF	443	480	1.47e-3	SMART
LamG	505	650	1.75e-9	SMART
EGF	674	707	6.5e-5	SMART
LamG	734	859	1.05e-7	SMART
EGF	889	922	1.19e-3	SMART
LamG	971	1104	6.85e-12	SMART
EGF	1141	1174	7.07e-6	SMART
EGF_CA	1176	1211	3.01e-9	SMART
EGF	1216	1249	3.57e-2	SMART
EGF	1257	1294	6.92e0	SMART
EGF_CA	1296	1332	4.19e-8	SMART
transmembrane domain	1346	1368	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000196402

SMART Domains

Protein: ENSMUSP00000142702
Gene: ENSMUSG00000063681

Domain	Start	End	E-Value	Type
low complexity region	8	19	N/A	INTRINSIC
EGF	72	107	5.97e-4	SMART
EGF	112	145	9.19e-5	SMART
EGF_CA	147	183	2.89e-11	SMART
EGF_CA	185	221	1.14e-9	SMART
EGF_CA	223	259	2.26e-13	SMART
EGF_CA	261	298	5.15e-8	SMART
EGF	303	336	8.12e-6	SMART
EGF	341	394	2.6e-4	SMART
EGF_CA	396	438	2.54e-7	SMART
EGF	443	480	1.47e-3	SMART
LamG	505	650	1.75e-9	SMART
EGF	674	707	6.5e-5	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000198445
AA Change: K719E

PolyPhen 2 Score 0.978 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000142552
Gene: ENSMUSG00000063681
AA Change: K719E

Domain	Start	End	E-Value	Type
low complexity region	8	19	N/A	INTRINSIC
EGF	72	107	5.97e-4	SMART
EGF	112	145	9.19e-5	SMART
EGF_CA	147	183	2.89e-11	SMART
EGF_CA	185	221	1.14e-9	SMART
EGF_CA	223	259	2.26e-13	SMART
EGF_CA	261	298	5.15e-8	SMART
EGF	303	333	1.63e1	SMART
EGF_CA	335	377	2.54e-7	SMART
EGF	382	419	1.47e-3	SMART
LamG	444	589	1.75e-9	SMART
EGF	613	646	6.5e-5	SMART
LamG	673	798	1.05e-7	SMART
EGF	828	861	1.19e-3	SMART
LamG	910	1043	6.85e-12	SMART
EGF	1080	1113	7.07e-6	SMART
EGF_CA	1115	1150	3.01e-9	SMART
EGF	1155	1188	3.57e-2	SMART
EGF	1196	1233	6.92e0	SMART
EGF_CA	1235	1271	4.19e-8	SMART
low complexity region	1282	1296	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

96% (73/76)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein which is similar to the Drosophila crumbs protein and localizes to the inner segment of mammalian photoreceptors. In Drosophila crumbs localizes to the stalk of the fly photoreceptor and may be a component of the molecular scaffold that controls proper development of polarity in the eye. Mutations in this gene are associated with a severe form of retinitis pigmentosa, RP12, and with Leber congenital amaurosis. Alternate splicing results in multiple transcript variants, some protein coding and some non-protein coding.[provided by RefSeq, Apr 2012]
PHENOTYPE: Homozygotes for a null allele show focal retinal lesions, loss of adherens junctions between photoreceptors and Muller glia cells, and light-accelerated retinal degeneration. Homozygotes for a spontaneous allele show background-sensitive retinal spotting, photoreceptor dysplasia and degeneration. [provided by MGI curators]

Allele List at MGI

All alleles(5) : Targeted(4) Spontaneous(1)

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	T	A	7: 41,275,837 (GRCm39)	N513K	possibly damaging	Het
Actl6a	T	A	3: 32,779,469 (GRCm39)	I399N	probably damaging	Het
Adamts5	A	G	16: 85,666,971 (GRCm39)	V507A	probably damaging	Het
Ap3d1	A	G	10: 80,554,918 (GRCm39)	V469A	probably benign	Het
Arhgef4	G	T	1: 34,764,241 (GRCm39)		probably null	Het
Arhgef4	A	T	1: 34,849,576 (GRCm39)	Q389L	probably benign	Het
Atad2	A	G	15: 57,980,194 (GRCm39)	V106A	possibly damaging	Het
Azin2	T	C	4: 128,841,341 (GRCm39)	E275G	probably damaging	Het
Bhlhe23	A	G	2: 180,418,143 (GRCm39)	S132P	probably damaging	Het
Capn12	A	G	7: 28,581,243 (GRCm39)	Y32C	probably damaging	Het
Celsr2	A	T	3: 108,321,349 (GRCm39)	S488T	probably benign	Het
Cenpx	A	G	11: 120,602,582 (GRCm39)	V27A	possibly damaging	Het
Clca3a2	G	A	3: 144,778,005 (GRCm39)	P760L	probably benign	Het
Clstn3	T	C	6: 124,408,571 (GRCm39)	S951G	probably benign	Het
Clybl	C	A	14: 122,621,670 (GRCm39)	P286Q	probably benign	Het
Cmya5	T	A	13: 93,226,866 (GRCm39)	M2741L	probably benign	Het
Cntnap2	G	A	6: 47,026,183 (GRCm39)	G944E	probably damaging	Het
Col8a1	A	C	16: 57,447,283 (GRCm39)	Y742*	probably null	Het
Crygc	T	A	1: 65,112,376 (GRCm39)	Y66F	probably benign	Het
Cyp2b9	G	A	7: 25,873,004 (GRCm39)	G49E	possibly damaging	Het
D130043K22Rik	T	C	13: 25,041,090 (GRCm39)	S171P	possibly damaging	Het
Dhx36	T	C	3: 62,378,895 (GRCm39)	I890V	probably benign	Het
Dpp9	C	T	17: 56,512,765 (GRCm39)	G148S	probably damaging	Het
Eml6	G	T	11: 29,781,175 (GRCm39)	S619Y	probably damaging	Het
Enpp4	A	T	17: 44,412,252 (GRCm39)	L319*	probably null	Het
Esyt1	T	C	10: 128,361,356 (GRCm39)	S113G	probably benign	Het
Fat1	A	G	8: 45,404,791 (GRCm39)	D514G	possibly damaging	Het
Fbxo16	G	A	14: 65,524,594 (GRCm39)	R38Q	probably damaging	Het
Fchsd1	A	T	18: 38,092,706 (GRCm39)	M668K	unknown	Het
Gpr149	C	T	3: 62,511,093 (GRCm39)	S302N	probably benign	Het
Gsap	A	G	5: 21,433,064 (GRCm39)	K258E	probably benign	Het
Helq	C	A	5: 100,939,218 (GRCm39)	V443F	probably benign	Het
Helq	T	A	5: 100,939,219 (GRCm39)	K442N	probably damaging	Het
Ighv1-37	T	C	12: 114,860,123 (GRCm39)	E29G	possibly damaging	Het
Ighv1-9	C	A	12: 114,547,620 (GRCm39)	M1I	probably null	Het
Itga6	T	G	2: 71,671,477 (GRCm39)	F743V	probably benign	Het
Lpin1	C	A	12: 16,588,548 (GRCm39)	D881Y		Het
Lypd8	T	A	11: 58,273,640 (GRCm39)	C40S	possibly damaging	Het
Macf1	C	T	4: 123,326,227 (GRCm39)	R5092Q	probably damaging	Het
Mcm7	A	G	5: 138,166,593 (GRCm39)		probably null	Het
Micall2	G	A	5: 139,696,170 (GRCm39)	T831I	unknown	Het
Myo18b	A	T	5: 113,022,927 (GRCm39)	M155K	unknown	Het
Nckap5	T	A	1: 125,867,928 (GRCm39)	H201L	unknown	Het
Ndst2	A	T	14: 20,779,240 (GRCm39)	D333E	possibly damaging	Het
Nlgn1	A	G	3: 25,966,804 (GRCm39)		probably null	Het
Nup188	T	G	2: 30,232,397 (GRCm39)	Y1483D	probably benign	Het
Or4c109	A	C	2: 88,818,057 (GRCm39)	L163*	probably null	Het
Or52ae9	T	A	7: 103,390,319 (GRCm39)	S43C	probably benign	Het
Or52n4b	A	C	7: 108,144,664 (GRCm39)	I311L	probably benign	Het
Or5p66	T	A	7: 107,885,526 (GRCm39)	Y269F	probably benign	Het
Or8g33	T	A	9: 39,337,635 (GRCm39)	H244L	probably damaging	Het
Peg3	A	C	7: 6,711,226 (GRCm39)	I1332S	possibly damaging	Het
Piezo2	T	C	18: 63,154,372 (GRCm39)	K2469R	probably damaging	Het
Pik3cg	T	C	12: 32,247,312 (GRCm39)	M804V	probably damaging	Het
Polq	A	T	16: 36,869,011 (GRCm39)	I794F	possibly damaging	Het
Rasgef1b	A	G	5: 99,370,191 (GRCm39)	V437A	probably benign	Het
Rdm1	T	A	11: 101,518,857 (GRCm39)	D21E	probably benign	Het
Ripk4	G	T	16: 97,551,311 (GRCm39)	Q219K	possibly damaging	Het
Ropn1l	T	C	15: 31,441,471 (GRCm39)	I217V		Het
Sdf2	T	C	11: 78,136,858 (GRCm39)	S13P	unknown	Het
Selenbp1	A	G	3: 94,847,079 (GRCm39)	T202A	probably benign	Het
Slfn5	T	C	11: 82,850,933 (GRCm39)	F410S	possibly damaging	Het
Srrm2	A	G	17: 24,039,880 (GRCm39)	T2175A	probably benign	Het
St6galnac3	T	C	3: 153,117,360 (GRCm39)	Y121C	possibly damaging	Het
Stat1	A	G	1: 52,184,337 (GRCm39)	N417S	probably benign	Het
Tead1	T	C	7: 112,475,378 (GRCm39)	F276L	probably damaging	Het
Teddm3	A	T	16: 20,971,737 (GRCm39)	S277R	probably benign	Het
Tmc2	A	G	2: 130,103,317 (GRCm39)		probably null	Het
Tmem156	G	A	5: 65,231,127 (GRCm39)	L248F	probably damaging	Het
Tnn	T	C	1: 159,953,986 (GRCm39)	S590G	probably benign	Het
Trmt44	A	G	5: 35,731,422 (GRCm39)		probably null	Het
Upp2	T	A	2: 58,668,022 (GRCm39)	Y238*	probably null	Het
Usp32	T	C	11: 84,930,838 (GRCm39)	T531A	probably damaging	Het
Vmn2r78	A	T	7: 86,569,431 (GRCm39)	D108V	probably benign	Het
Wdfy3	G	T	5: 102,078,830 (GRCm39)	A824E	probably benign	Het
Xylt1	T	C	7: 117,255,870 (GRCm39)	V814A	probably benign	Het
Zfy1	C	T	Y: 732,990 (GRCm39)	R281H	unknown	Het

Other mutations in Crb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Crb1	APN	1	139,250,983 (GRCm39)	missense	probably benign	0.16
IGL01591:Crb1	APN	1	139,165,077 (GRCm39)	missense	probably damaging	1.00
IGL01644:Crb1	APN	1	139,165,368 (GRCm39)	nonsense	probably null
IGL01769:Crb1	APN	1	139,264,806 (GRCm39)	missense	probably damaging	1.00
IGL02172:Crb1	APN	1	139,164,965 (GRCm39)	missense	probably damaging	1.00
IGL02294:Crb1	APN	1	139,162,520 (GRCm39)	missense	possibly damaging	0.89
IGL02382:Crb1	APN	1	139,165,352 (GRCm39)	missense	probably damaging	0.99
IGL02411:Crb1	APN	1	139,176,213 (GRCm39)	missense	probably damaging	1.00
IGL03070:Crb1	APN	1	139,168,996 (GRCm39)	missense	possibly damaging	0.79
IGL02984:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL02988:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL02991:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL02991:Crb1	UTSW	1	139,164,822 (GRCm39)	frame shift	probably null
IGL03014:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03050:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03054:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03055:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03097:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03098:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03134:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03138:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
IGL03147:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
P0017:Crb1	UTSW	1	139,176,678 (GRCm39)	missense	possibly damaging	0.64
R0276:Crb1	UTSW	1	139,251,073 (GRCm39)	missense	possibly damaging	0.85
R0325:Crb1	UTSW	1	139,168,904 (GRCm39)	missense	probably damaging	1.00
R0401:Crb1	UTSW	1	139,126,529 (GRCm39)	splice site	probably benign
R0479:Crb1	UTSW	1	139,126,352 (GRCm39)	missense	probably damaging	0.98
R0734:Crb1	UTSW	1	139,264,822 (GRCm39)	missense	probably benign	0.25
R1573:Crb1	UTSW	1	139,265,344 (GRCm39)	missense	probably damaging	1.00
R1728:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1728:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1728:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1728:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1728:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1729:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1729:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1729:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1729:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1729:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1730:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1730:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1730:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1730:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1730:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1739:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1739:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1739:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1739:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1739:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1762:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1762:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1762:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1762:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1762:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1762:Crb1	UTSW	1	139,165,269 (GRCm39)	missense	probably damaging	1.00
R1783:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1783:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1783:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1783:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1783:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1784:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1784:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1784:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1784:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1784:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1785:Crb1	UTSW	1	139,170,733 (GRCm39)	missense	probably damaging	1.00
R1785:Crb1	UTSW	1	139,171,155 (GRCm39)	missense	probably benign	0.00
R1785:Crb1	UTSW	1	139,162,517 (GRCm39)	missense	probably benign
R1785:Crb1	UTSW	1	139,165,360 (GRCm39)	missense	probably benign	0.00
R1785:Crb1	UTSW	1	139,168,876 (GRCm39)	missense	probably damaging	1.00
R1848:Crb1	UTSW	1	139,164,750 (GRCm39)	missense	probably damaging	0.97
R1894:Crb1	UTSW	1	139,170,931 (GRCm39)	missense	probably benign	0.02
R2057:Crb1	UTSW	1	139,242,488 (GRCm39)	missense	probably damaging	1.00
R2136:Crb1	UTSW	1	139,265,163 (GRCm39)	missense	probably benign	0.03
R2140:Crb1	UTSW	1	139,164,750 (GRCm39)	missense	probably benign	0.01
R2363:Crb1	UTSW	1	139,265,016 (GRCm39)	missense	possibly damaging	0.89
R3605:Crb1	UTSW	1	139,165,077 (GRCm39)	missense	probably damaging	1.00
R3817:Crb1	UTSW	1	139,175,835 (GRCm39)	missense	probably benign
R3942:Crb1	UTSW	1	139,265,211 (GRCm39)	missense	possibly damaging	0.49
R4272:Crb1	UTSW	1	139,251,049 (GRCm39)	missense	probably benign	0.04
R4301:Crb1	UTSW	1	139,176,568 (GRCm39)	missense	probably benign	0.01
R4403:Crb1	UTSW	1	139,176,117 (GRCm39)	missense	probably benign	0.00
R4700:Crb1	UTSW	1	139,126,509 (GRCm39)	missense	probably damaging	0.96
R4771:Crb1	UTSW	1	139,255,942 (GRCm39)	missense	probably damaging	1.00
R4845:Crb1	UTSW	1	139,170,772 (GRCm39)	missense	probably benign	0.06
R4867:Crb1	UTSW	1	139,170,752 (GRCm39)	missense	probably damaging	1.00
R5159:Crb1	UTSW	1	139,170,756 (GRCm39)	missense	probably damaging	0.99
R5270:Crb1	UTSW	1	139,164,602 (GRCm39)	missense	probably damaging	0.97
R5347:Crb1	UTSW	1	139,265,109 (GRCm39)	missense	probably damaging	1.00
R5513:Crb1	UTSW	1	139,164,559 (GRCm39)	critical splice donor site	probably null
R5641:Crb1	UTSW	1	139,176,627 (GRCm39)	missense	probably damaging	0.99
R5754:Crb1	UTSW	1	139,159,337 (GRCm39)	missense	probably damaging	1.00
R5968:Crb1	UTSW	1	139,170,739 (GRCm39)	missense	probably damaging	1.00
R6122:Crb1	UTSW	1	139,176,686 (GRCm39)	nonsense	probably null
R6369:Crb1	UTSW	1	139,165,200 (GRCm39)	missense	probably damaging	1.00
R6809:Crb1	UTSW	1	139,170,864 (GRCm39)	missense	probably benign	0.00
R7020:Crb1	UTSW	1	139,159,341 (GRCm39)	missense	possibly damaging	0.85
R7072:Crb1	UTSW	1	139,165,013 (GRCm39)	missense	probably damaging	1.00
R7073:Crb1	UTSW	1	139,176,049 (GRCm39)	missense	probably damaging	0.99
R7135:Crb1	UTSW	1	139,171,105 (GRCm39)	missense	probably damaging	0.97
R7493:Crb1	UTSW	1	139,164,768 (GRCm39)	missense	probably damaging	1.00
R7539:Crb1	UTSW	1	139,175,967 (GRCm39)	missense	probably damaging	1.00
R7554:Crb1	UTSW	1	139,265,019 (GRCm39)	missense	probably damaging	1.00
R7593:Crb1	UTSW	1	139,164,978 (GRCm39)	missense	probably damaging	1.00
R7740:Crb1	UTSW	1	139,165,428 (GRCm39)	missense	probably benign	0.01
R7912:Crb1	UTSW	1	139,170,909 (GRCm39)	missense	probably damaging	1.00
R8036:Crb1	UTSW	1	139,165,122 (GRCm39)	missense	probably benign	0.07
R8042:Crb1	UTSW	1	139,242,392 (GRCm39)	missense	probably damaging	0.99
R8329:Crb1	UTSW	1	139,165,005 (GRCm39)	missense	probably damaging	1.00
R8332:Crb1	UTSW	1	139,165,152 (GRCm39)	missense	probably damaging	0.96
R8880:Crb1	UTSW	1	139,164,886 (GRCm39)	missense	probably benign	0.19
R8894:Crb1	UTSW	1	139,175,750 (GRCm39)	missense	possibly damaging	0.95
R9052:Crb1	UTSW	1	139,171,161 (GRCm39)	missense	possibly damaging	0.50
R9138:Crb1	UTSW	1	139,162,468 (GRCm39)	missense
R9567:Crb1	UTSW	1	139,171,208 (GRCm39)	missense	probably benign	0.04
X0066:Crb1	UTSW	1	139,175,983 (GRCm39)	missense	probably benign	0.10
Z1176:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null
Z1177:Crb1	UTSW	1	139,264,766 (GRCm39)	critical splice donor site	probably null
Z1177:Crb1	UTSW	1	139,176,639 (GRCm39)	missense	possibly damaging	0.80
Z1177:Crb1	UTSW	1	139,164,824 (GRCm39)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- CTCTCTGTCAGGTAAGCCAC -3'
(R):5'- GGACATTGCAGAGTATGTAGCG -3'

Sequencing Primer
(F):5'- ACCAATGAAGATGACGTCTCCTCTTC -3'
(R):5'- TTTGGCCAAGATGACTCCACAGG -3'

Posted On

2022-02-07