Mutagenetix > Incidental Mutation

Incidental Mutation 'R9209:Micall2'

698705

Institutional Source

Beutler Lab

Gene Symbol

Micall2

Ensembl Gene

ENSMUSG00000036718

Gene Name

MICAL-like 2

Synonyms

MICAL-L2, Jrab, A930021H16Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.352) question?

Stock #

R9209 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

139692451-139722091 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 139696170 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 831 (T831I)

Ref Sequence

ENSEMBL: ENSMUSP00000039707 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044642] [ENSMUST00000170773]

AlphaFold

Q3TN34

Predicted Effect

unknown
Transcript: ENSMUST00000044642
AA Change: T831I

SMART Domains

Protein: ENSMUSP00000039707
Gene: ENSMUSG00000036718
AA Change: T831I

Domain	Start	End	E-Value	Type
CH	3	102	4.34e-20	SMART
LIM	187	241	1.62e-5	SMART
low complexity region	242	253	N/A	INTRINSIC
low complexity region	326	344	N/A	INTRINSIC
low complexity region	428	489	N/A	INTRINSIC
low complexity region	502	518	N/A	INTRINSIC
low complexity region	728	746	N/A	INTRINSIC
low complexity region	755	770	N/A	INTRINSIC
DUF3585	840	980	3.1e-63	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000165645

Predicted Effect

unknown
Transcript: ENSMUST00000170773
AA Change: T748I

SMART Domains

Protein: ENSMUSP00000127937
Gene: ENSMUSG00000036718
AA Change: T748I

Domain	Start	End	E-Value	Type
SCOP:d1bkra_	1	25	9e-5	SMART
Blast:DUF3585	1	45	2e-7	BLAST
LIM	104	158	1.62e-5	SMART
low complexity region	159	170	N/A	INTRINSIC
low complexity region	243	261	N/A	INTRINSIC
low complexity region	345	406	N/A	INTRINSIC
low complexity region	419	435	N/A	INTRINSIC
low complexity region	645	663	N/A	INTRINSIC
low complexity region	672	687	N/A	INTRINSIC
DUF3585	757	897	3.1e-63	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

96% (73/76)

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	T	A	7: 41,275,837 (GRCm39)	N513K	possibly damaging	Het
Actl6a	T	A	3: 32,779,469 (GRCm39)	I399N	probably damaging	Het
Adamts5	A	G	16: 85,666,971 (GRCm39)	V507A	probably damaging	Het
Ap3d1	A	G	10: 80,554,918 (GRCm39)	V469A	probably benign	Het
Arhgef4	G	T	1: 34,764,241 (GRCm39)		probably null	Het
Arhgef4	A	T	1: 34,849,576 (GRCm39)	Q389L	probably benign	Het
Atad2	A	G	15: 57,980,194 (GRCm39)	V106A	possibly damaging	Het
Azin2	T	C	4: 128,841,341 (GRCm39)	E275G	probably damaging	Het
Bhlhe23	A	G	2: 180,418,143 (GRCm39)	S132P	probably damaging	Het
Capn12	A	G	7: 28,581,243 (GRCm39)	Y32C	probably damaging	Het
Celsr2	A	T	3: 108,321,349 (GRCm39)	S488T	probably benign	Het
Cenpx	A	G	11: 120,602,582 (GRCm39)	V27A	possibly damaging	Het
Clca3a2	G	A	3: 144,778,005 (GRCm39)	P760L	probably benign	Het
Clstn3	T	C	6: 124,408,571 (GRCm39)	S951G	probably benign	Het
Clybl	C	A	14: 122,621,670 (GRCm39)	P286Q	probably benign	Het
Cmya5	T	A	13: 93,226,866 (GRCm39)	M2741L	probably benign	Het
Cntnap2	G	A	6: 47,026,183 (GRCm39)	G944E	probably damaging	Het
Col8a1	A	C	16: 57,447,283 (GRCm39)	Y742*	probably null	Het
Crb1	T	C	1: 139,171,051 (GRCm39)	K780E	probably damaging	Het
Crygc	T	A	1: 65,112,376 (GRCm39)	Y66F	probably benign	Het
Cyp2b9	G	A	7: 25,873,004 (GRCm39)	G49E	possibly damaging	Het
D130043K22Rik	T	C	13: 25,041,090 (GRCm39)	S171P	possibly damaging	Het
Dhx36	T	C	3: 62,378,895 (GRCm39)	I890V	probably benign	Het
Dpp9	C	T	17: 56,512,765 (GRCm39)	G148S	probably damaging	Het
Eml6	G	T	11: 29,781,175 (GRCm39)	S619Y	probably damaging	Het
Enpp4	A	T	17: 44,412,252 (GRCm39)	L319*	probably null	Het
Esyt1	T	C	10: 128,361,356 (GRCm39)	S113G	probably benign	Het
Fat1	A	G	8: 45,404,791 (GRCm39)	D514G	possibly damaging	Het
Fbxo16	G	A	14: 65,524,594 (GRCm39)	R38Q	probably damaging	Het
Fchsd1	A	T	18: 38,092,706 (GRCm39)	M668K	unknown	Het
Gpr149	C	T	3: 62,511,093 (GRCm39)	S302N	probably benign	Het
Gsap	A	G	5: 21,433,064 (GRCm39)	K258E	probably benign	Het
Helq	C	A	5: 100,939,218 (GRCm39)	V443F	probably benign	Het
Helq	T	A	5: 100,939,219 (GRCm39)	K442N	probably damaging	Het
Ighv1-37	T	C	12: 114,860,123 (GRCm39)	E29G	possibly damaging	Het
Ighv1-9	C	A	12: 114,547,620 (GRCm39)	M1I	probably null	Het
Itga6	T	G	2: 71,671,477 (GRCm39)	F743V	probably benign	Het
Lpin1	C	A	12: 16,588,548 (GRCm39)	D881Y		Het
Lypd8	T	A	11: 58,273,640 (GRCm39)	C40S	possibly damaging	Het
Macf1	C	T	4: 123,326,227 (GRCm39)	R5092Q	probably damaging	Het
Mcm7	A	G	5: 138,166,593 (GRCm39)		probably null	Het
Myo18b	A	T	5: 113,022,927 (GRCm39)	M155K	unknown	Het
Nckap5	T	A	1: 125,867,928 (GRCm39)	H201L	unknown	Het
Ndst2	A	T	14: 20,779,240 (GRCm39)	D333E	possibly damaging	Het
Nlgn1	A	G	3: 25,966,804 (GRCm39)		probably null	Het
Nup188	T	G	2: 30,232,397 (GRCm39)	Y1483D	probably benign	Het
Or4c109	A	C	2: 88,818,057 (GRCm39)	L163*	probably null	Het
Or52ae9	T	A	7: 103,390,319 (GRCm39)	S43C	probably benign	Het
Or52n4b	A	C	7: 108,144,664 (GRCm39)	I311L	probably benign	Het
Or5p66	T	A	7: 107,885,526 (GRCm39)	Y269F	probably benign	Het
Or8g33	T	A	9: 39,337,635 (GRCm39)	H244L	probably damaging	Het
Peg3	A	C	7: 6,711,226 (GRCm39)	I1332S	possibly damaging	Het
Piezo2	T	C	18: 63,154,372 (GRCm39)	K2469R	probably damaging	Het
Pik3cg	T	C	12: 32,247,312 (GRCm39)	M804V	probably damaging	Het
Polq	A	T	16: 36,869,011 (GRCm39)	I794F	possibly damaging	Het
Rasgef1b	A	G	5: 99,370,191 (GRCm39)	V437A	probably benign	Het
Rdm1	T	A	11: 101,518,857 (GRCm39)	D21E	probably benign	Het
Ripk4	G	T	16: 97,551,311 (GRCm39)	Q219K	possibly damaging	Het
Ropn1l	T	C	15: 31,441,471 (GRCm39)	I217V		Het
Sdf2	T	C	11: 78,136,858 (GRCm39)	S13P	unknown	Het
Selenbp1	A	G	3: 94,847,079 (GRCm39)	T202A	probably benign	Het
Slfn5	T	C	11: 82,850,933 (GRCm39)	F410S	possibly damaging	Het
Srrm2	A	G	17: 24,039,880 (GRCm39)	T2175A	probably benign	Het
St6galnac3	T	C	3: 153,117,360 (GRCm39)	Y121C	possibly damaging	Het
Stat1	A	G	1: 52,184,337 (GRCm39)	N417S	probably benign	Het
Tead1	T	C	7: 112,475,378 (GRCm39)	F276L	probably damaging	Het
Teddm3	A	T	16: 20,971,737 (GRCm39)	S277R	probably benign	Het
Tmc2	A	G	2: 130,103,317 (GRCm39)		probably null	Het
Tmem156	G	A	5: 65,231,127 (GRCm39)	L248F	probably damaging	Het
Tnn	T	C	1: 159,953,986 (GRCm39)	S590G	probably benign	Het
Trmt44	A	G	5: 35,731,422 (GRCm39)		probably null	Het
Upp2	T	A	2: 58,668,022 (GRCm39)	Y238*	probably null	Het
Usp32	T	C	11: 84,930,838 (GRCm39)	T531A	probably damaging	Het
Vmn2r78	A	T	7: 86,569,431 (GRCm39)	D108V	probably benign	Het
Wdfy3	G	T	5: 102,078,830 (GRCm39)	A824E	probably benign	Het
Xylt1	T	C	7: 117,255,870 (GRCm39)	V814A	probably benign	Het
Zfy1	C	T	Y: 732,990 (GRCm39)	R281H	unknown	Het

Other mutations in Micall2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00231:Micall2	APN	5	139,703,311 (GRCm39)	critical splice acceptor site	probably null
IGL00496:Micall2	APN	5	139,702,083 (GRCm39)	missense	probably benign	0.01
IGL02641:Micall2	APN	5	139,705,094 (GRCm39)	missense	probably damaging	1.00
IGL03245:Micall2	APN	5	139,705,014 (GRCm39)	missense	probably damaging	1.00
IGL03252:Micall2	APN	5	139,702,481 (GRCm39)	missense	probably benign	0.01
R1214:Micall2	UTSW	5	139,697,396 (GRCm39)	missense	probably damaging	0.97
R1468:Micall2	UTSW	5	139,705,097 (GRCm39)	missense	probably damaging	1.00
R1468:Micall2	UTSW	5	139,705,097 (GRCm39)	missense	probably damaging	1.00
R1831:Micall2	UTSW	5	139,702,508 (GRCm39)	missense	probably benign	0.09
R1833:Micall2	UTSW	5	139,702,508 (GRCm39)	missense	probably benign	0.09
R1969:Micall2	UTSW	5	139,721,885 (GRCm39)	missense	probably damaging	1.00
R2023:Micall2	UTSW	5	139,703,266 (GRCm39)	missense	possibly damaging	0.51
R2060:Micall2	UTSW	5	139,697,317 (GRCm39)	missense	probably damaging	0.98
R2330:Micall2	UTSW	5	139,703,270 (GRCm39)	missense	probably damaging	1.00
R3820:Micall2	UTSW	5	139,701,611 (GRCm39)	missense	possibly damaging	0.92
R4299:Micall2	UTSW	5	139,695,226 (GRCm39)	intron	probably benign
R4334:Micall2	UTSW	5	139,699,105 (GRCm39)	missense	probably damaging	1.00
R4451:Micall2	UTSW	5	139,692,852 (GRCm39)	missense	probably damaging	1.00
R4769:Micall2	UTSW	5	139,692,641 (GRCm39)	missense	probably damaging	0.97
R4911:Micall2	UTSW	5	139,702,580 (GRCm39)	missense	probably damaging	1.00
R4996:Micall2	UTSW	5	139,696,344 (GRCm39)	missense	probably benign	0.31
R5118:Micall2	UTSW	5	139,702,202 (GRCm39)	missense	probably damaging	1.00
R5155:Micall2	UTSW	5	139,695,986 (GRCm39)	missense	probably damaging	1.00
R5475:Micall2	UTSW	5	139,702,224 (GRCm39)	missense	probably damaging	1.00
R5750:Micall2	UTSW	5	139,701,456 (GRCm39)	splice site	probably null
R5998:Micall2	UTSW	5	139,692,666 (GRCm39)	critical splice acceptor site	probably null
R6181:Micall2	UTSW	5	139,702,506 (GRCm39)	missense	probably benign	0.41
R6852:Micall2	UTSW	5	139,701,548 (GRCm39)	missense	possibly damaging	0.90
R7046:Micall2	UTSW	5	139,694,699 (GRCm39)	unclassified	probably benign
R7395:Micall2	UTSW	5	139,702,124 (GRCm39)	missense	possibly damaging	0.78
R8514:Micall2	UTSW	5	139,701,977 (GRCm39)	missense	probably damaging	1.00
R8889:Micall2	UTSW	5	139,703,254 (GRCm39)	missense	probably damaging	0.98
R8892:Micall2	UTSW	5	139,703,254 (GRCm39)	missense	probably damaging	0.98
R8960:Micall2	UTSW	5	139,702,025 (GRCm39)	missense	probably benign	0.23
R9060:Micall2	UTSW	5	139,705,035 (GRCm39)	missense	probably damaging	1.00
R9227:Micall2	UTSW	5	139,701,827 (GRCm39)	missense	unknown
R9230:Micall2	UTSW	5	139,701,827 (GRCm39)	missense	unknown
R9260:Micall2	UTSW	5	139,695,453 (GRCm39)	missense	unknown
R9452:Micall2	UTSW	5	139,703,311 (GRCm39)	critical splice acceptor site	probably null
Z1088:Micall2	UTSW	5	139,702,050 (GRCm39)	missense	probably benign	0.12
Z1088:Micall2	UTSW	5	139,692,649 (GRCm39)	missense	probably damaging	1.00
Z1177:Micall2	UTSW	5	139,696,057 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- AAAGCTGACAGAGTGTGGCC -3'
(R):5'- TCAGTTCCAGGGATGCTTGG -3'

Sequencing Primer
(F):5'- ACAGAGTGTGGCCATGGTC -3'
(R):5'- CCAGGGATGCTTGGAGGGG -3'

Posted On

2022-02-07