Mutagenetix > Incidental Mutation

Incidental Mutation 'R9221:Vmn2r5'

699443

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r5

Ensembl Gene

ENSMUSG00000068999

Gene Name

vomeronasal 2, receptor 5

Synonyms

EG667060

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.120) question?

Stock #

R9221 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64398242-64417156 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 64411721 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 282 (Y282*)

Ref Sequence

ENSEMBL: ENSMUSP00000135592 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170270] [ENSMUST00000177184]

AlphaFold

K7N788

Predicted Effect

probably null
Transcript: ENSMUST00000170270
AA Change: Y195*

SMART Domains

Protein: ENSMUSP00000131925
Gene: ENSMUSG00000068999
AA Change: Y195*

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	9.9e-78	PFAM
Pfam:NCD3G	458	511	8.5e-18	PFAM
Pfam:7tm_3	542	779	2.3e-74	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000177184
AA Change: Y282*

SMART Domains

Protein: ENSMUSP00000135592
Gene: ENSMUSG00000068999
AA Change: Y282*

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	86	503	1.8e-80	PFAM
Pfam:NCD3G	545	598	1e-14	PFAM
Pfam:7tm_3	631	865	1.4e-45	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	A	G	3: 121,921,828 (GRCm39)	D1128G	probably damaging	Het
Acsm3	A	T	7: 119,368,131 (GRCm39)	R116*	probably null	Het
Acte1	G	T	7: 143,434,902 (GRCm39)		probably null	Het
Ahnak	T	A	19: 8,989,943 (GRCm39)	D3742E	probably damaging	Het
Arhgef17	A	G	7: 100,528,818 (GRCm39)	S598P	possibly damaging	Het
Asxl2	T	C	12: 3,552,310 (GRCm39)	F1351L	probably damaging	Het
Atg7	C	T	6: 114,672,588 (GRCm39)	T267I	possibly damaging	Het
Bace2	A	G	16: 97,209,692 (GRCm39)	K202R	probably benign	Het
Bccip	G	T	7: 133,311,249 (GRCm39)	V55L	probably benign	Het
Bpifa1	T	A	2: 153,988,052 (GRCm39)	H198Q	possibly damaging	Het
Btbd7	A	G	12: 102,777,430 (GRCm39)	Y466H	probably damaging	Het
Cdc45	A	C	16: 18,605,521 (GRCm39)	S480A	probably benign	Het
Cenpe	T	C	3: 134,935,839 (GRCm39)	Y425H	possibly damaging	Het
Cep76	A	C	18: 67,767,977 (GRCm39)	M185R	probably damaging	Het
Chd5	G	T	4: 152,456,122 (GRCm39)	M930I	probably damaging	Het
Clstn2	G	T	9: 97,343,395 (GRCm39)	T684K	probably benign	Het
Col4a2	A	G	8: 11,491,943 (GRCm39)	N1270S	possibly damaging	Het
Crxos	A	G	7: 15,636,850 (GRCm39)	H141R	probably benign	Het
Dcc	A	G	18: 71,553,433 (GRCm39)	I741T	possibly damaging	Het
Ddx20	A	T	3: 105,587,685 (GRCm39)	C430*	probably null	Het
Dgki	G	A	6: 37,273,615 (GRCm39)	T12M	probably benign	Het
Dock6	T	C	9: 21,721,153 (GRCm39)	N1676S	possibly damaging	Het
Exosc6	G	A	8: 111,783,028 (GRCm39)	R9H	probably damaging	Het
Fstl5	A	G	3: 76,569,114 (GRCm39)	Q589R	probably damaging	Het
Gm4846	T	A	1: 166,324,959 (GRCm39)	Y44F	probably benign	Het
Gpr15lg	A	G	14: 36,829,352 (GRCm39)	S44P	probably damaging	Het
Gtf3c2	A	C	5: 31,326,401 (GRCm39)	I370R	probably damaging	Het
Hjurp	CTCTGGGAGGGCTTGCTCCGGGGGCAGTGTGTCCTGTTCTTGTGCAGCCCCT	C	1: 88,193,999 (GRCm39)		probably benign	Het
Hspg2	A	G	4: 137,287,726 (GRCm39)	M3696V	possibly damaging	Het
Itga1	A	T	13: 115,166,695 (GRCm39)	C167*	probably null	Het
Khdc4	T	C	3: 88,593,853 (GRCm39)	S81P	probably benign	Het
Kpna2	A	G	11: 106,880,158 (GRCm39)	S497P	probably damaging	Het
Krt77	T	A	15: 101,774,064 (GRCm39)	T197S	probably damaging	Het
Krtap6-5	T	C	16: 88,844,655 (GRCm39)	Y26C	unknown	Het
Lhfpl5	A	T	17: 28,799,133 (GRCm39)	D214V	possibly damaging	Het
Lrrtm1	A	G	6: 77,221,596 (GRCm39)	E351G	probably damaging	Het
Mphosph9	A	G	5: 124,403,427 (GRCm39)	V867A	probably benign	Het
Mtcl1	T	A	17: 66,650,879 (GRCm39)	M1529L	probably benign	Het
Mthfr	A	T	4: 148,132,626 (GRCm39)	Q268L	probably damaging	Het
Myo1d	A	T	11: 80,565,744 (GRCm39)	N360K	probably damaging	Het
Nbea	AC	A	3: 55,998,393 (GRCm39)		probably null	Het
Or1e33	A	T	11: 73,738,108 (GRCm39)	V281E	probably damaging	Het
Or4p8	T	C	2: 88,727,255 (GRCm39)	S229G	probably benign	Het
Or5an1b	A	T	19: 12,299,336 (GRCm39)	M285K	probably damaging	Het
Or5m11	T	A	2: 85,782,185 (GRCm39)	Y259*	probably null	Het
P2rx5	A	T	11: 73,062,655 (GRCm39)	T455S	probably damaging	Het
Palld	A	T	8: 61,969,591 (GRCm39)	F1244L	unknown	Het
Pbld1	A	G	10: 62,907,829 (GRCm39)	T125A		Het
Pde3b	G	A	7: 114,014,697 (GRCm39)		probably benign	Het
Pfkm	T	C	15: 98,019,188 (GRCm39)	S180P	probably damaging	Het
Pla2g4d	T	A	2: 120,100,453 (GRCm39)	R626S	possibly damaging	Het
Postn	A	G	3: 54,282,515 (GRCm39)	E492G	possibly damaging	Het
Prph2	A	G	17: 47,230,818 (GRCm39)	D237G	probably damaging	Het
Psmd4	T	C	3: 94,942,604 (GRCm39)	H105R	probably damaging	Het
Pygl	T	C	12: 70,242,401 (GRCm39)	N685S	probably damaging	Het
Rp1	A	G	1: 4,315,266 (GRCm39)	F502S	unknown	Het
Sik1	T	C	17: 32,066,167 (GRCm39)	I607V	probably benign	Het
Slc26a3	T	A	12: 31,513,470 (GRCm39)	I464N	possibly damaging	Het
Slc38a9	A	G	13: 112,825,910 (GRCm39)	N116S	probably damaging	Het
Sorcs2	C	A	5: 36,181,910 (GRCm39)		probably null	Het
Spink5	T	C	18: 44,119,367 (GRCm39)	L226P	probably damaging	Het
Tab1	G	A	15: 80,034,754 (GRCm39)	V180I	probably benign	Het
Tacc2	T	G	7: 130,226,058 (GRCm39)	S914R	probably damaging	Het
Tacc2	C	T	7: 130,226,209 (GRCm39)	R965C	probably benign	Het
Tlr9	A	G	9: 106,101,972 (GRCm39)	D421G	probably damaging	Het
Tmem181a	T	C	17: 6,307,265 (GRCm39)	L16P	probably damaging	Het
Trim58	A	T	11: 58,542,075 (GRCm39)	H345L	probably damaging	Het
Ttc13	G	A	8: 125,400,290 (GRCm39)	R688C	probably benign	Het
Ube2o	C	A	11: 116,433,664 (GRCm39)	V685L	probably damaging	Het
Uso1	A	G	5: 92,335,173 (GRCm39)	H511R	probably benign	Het

Other mutations in Vmn2r5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00742:Vmn2r5	APN	3	64,398,834 (GRCm39)	missense	possibly damaging	0.67
IGL01139:Vmn2r5	APN	3	64,398,826 (GRCm39)	missense	probably benign	0.28
IGL03293:Vmn2r5	APN	3	64,398,747 (GRCm39)	missense	probably benign	0.01
PIT4581001:Vmn2r5	UTSW	3	64,411,348 (GRCm39)	nonsense	probably null
R0063:Vmn2r5	UTSW	3	64,411,221 (GRCm39)	missense	probably benign	0.01
R0142:Vmn2r5	UTSW	3	64,400,009 (GRCm39)	missense	probably damaging	1.00
R0193:Vmn2r5	UTSW	3	64,398,951 (GRCm39)	missense	possibly damaging	0.91
R0219:Vmn2r5	UTSW	3	64,411,734 (GRCm39)	missense	probably damaging	0.99
R0313:Vmn2r5	UTSW	3	64,411,248 (GRCm39)	missense	probably benign
R0620:Vmn2r5	UTSW	3	64,411,235 (GRCm39)	nonsense	probably null
R0726:Vmn2r5	UTSW	3	64,411,186 (GRCm39)	missense	probably benign	0.01
R1073:Vmn2r5	UTSW	3	64,398,726 (GRCm39)	nonsense	probably null
R1549:Vmn2r5	UTSW	3	64,411,421 (GRCm39)	missense	probably damaging	1.00
R1581:Vmn2r5	UTSW	3	64,398,640 (GRCm39)	missense	probably damaging	1.00
R1624:Vmn2r5	UTSW	3	64,417,116 (GRCm39)	missense	probably benign	0.19
R1973:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1974:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1975:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R1977:Vmn2r5	UTSW	3	64,411,642 (GRCm39)	missense	probably damaging	1.00
R2189:Vmn2r5	UTSW	3	64,417,014 (GRCm39)	missense	probably benign	0.27
R2484:Vmn2r5	UTSW	3	64,411,392 (GRCm39)	missense	possibly damaging	0.94
R4573:Vmn2r5	UTSW	3	64,411,339 (GRCm39)	missense	probably damaging	0.98
R5243:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5384:Vmn2r5	UTSW	3	64,416,931 (GRCm39)	missense	probably benign	0.02
R5385:Vmn2r5	UTSW	3	64,416,931 (GRCm39)	missense	probably benign	0.02
R5587:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5588:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5589:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5590:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R5739:Vmn2r5	UTSW	3	64,411,497 (GRCm39)	missense	probably damaging	1.00
R6113:Vmn2r5	UTSW	3	64,398,820 (GRCm39)	missense	probably benign	0.03
R6739:Vmn2r5	UTSW	3	64,398,637 (GRCm39)	missense	probably damaging	0.97
R7106:Vmn2r5	UTSW	3	64,399,104 (GRCm39)	missense	probably benign	0.05
R7304:Vmn2r5	UTSW	3	64,411,671 (GRCm39)	missense	probably damaging	0.97
R7402:Vmn2r5	UTSW	3	64,403,176 (GRCm39)	missense	probably benign	0.05
R7477:Vmn2r5	UTSW	3	64,399,060 (GRCm39)	missense	probably damaging	1.00
R7571:Vmn2r5	UTSW	3	64,411,825 (GRCm39)	missense	probably damaging	1.00
R7678:Vmn2r5	UTSW	3	64,416,943 (GRCm39)	missense	probably benign
R7874:Vmn2r5	UTSW	3	64,398,453 (GRCm39)	missense	probably damaging	1.00
R8110:Vmn2r5	UTSW	3	64,398,709 (GRCm39)	missense	probably benign	0.00
R8223:Vmn2r5	UTSW	3	64,398,726 (GRCm39)	nonsense	probably null
R8767:Vmn2r5	UTSW	3	64,415,103 (GRCm39)	missense	possibly damaging	0.79
R8896:Vmn2r5	UTSW	3	64,411,203 (GRCm39)	missense	probably benign	0.00
R8948:Vmn2r5	UTSW	3	64,398,522 (GRCm39)	missense	probably damaging	1.00
R8950:Vmn2r5	UTSW	3	64,398,522 (GRCm39)	missense	probably damaging	1.00
R8962:Vmn2r5	UTSW	3	64,398,564 (GRCm39)	missense	probably damaging	1.00
R9012:Vmn2r5	UTSW	3	64,411,915 (GRCm39)	missense	probably damaging	1.00
R9109:Vmn2r5	UTSW	3	64,411,411 (GRCm39)	missense	possibly damaging	0.58
R9126:Vmn2r5	UTSW	3	64,399,159 (GRCm39)	missense	probably benign	0.13
R9192:Vmn2r5	UTSW	3	64,398,938 (GRCm39)	nonsense	probably null
R9375:Vmn2r5	UTSW	3	64,411,316 (GRCm39)	missense	probably damaging	1.00
R9529:Vmn2r5	UTSW	3	64,400,018 (GRCm39)	missense	probably benign	0.06
Z1088:Vmn2r5	UTSW	3	64,416,963 (GRCm39)	missense	probably benign	0.02
Z1177:Vmn2r5	UTSW	3	64,398,472 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTCCTGGTATAACACTTCTCGG -3'
(R):5'- AGGCCATGGTGACTCTCATC -3'

Sequencing Primer
(F):5'- GGTATAACACTTCTCGGTATTGC -3'
(R):5'- GACTCTCATCATACACTTTGGTTGGG -3'

Posted On

2022-02-07