Mutagenetix > Incidental Mutation

Incidental Mutation 'R9230:Or51a25'

700171

Institutional Source

Beutler Lab

Gene Symbol

Or51a25

Ensembl Gene

ENSMUSG00000066272

Gene Name

olfactory receptor family 51 subfamily A member 25

Synonyms

MOR11-1, Olfr559, GA_x6K02T2PBJ9-5441154-5440198

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R9230 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

102372739-102373695 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 102372795 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Phenylalanine at position 301 (V301F)

Ref Sequence

ENSEMBL: ENSMUSP00000151100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084816] [ENSMUST00000215657] [ENSMUST00000216312]

AlphaFold

Q8VH14

Predicted Effect

possibly damaging
Transcript: ENSMUST00000084816
AA Change: V301F

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000095820
Gene: ENSMUSG00000066272
AA Change: V301F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	317	1.1e-117	PFAM
Pfam:7TM_GPCR_Srsx	41	215	1e-10	PFAM
Pfam:7tm_1	47	299	3e-18	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215657
AA Change: V301F

PolyPhen 2 Score 0.948 (Sensitivity: 0.79; Specificity: 0.95)

Predicted Effect

probably benign
Transcript: ENSMUST00000216312

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (68/68)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adarb1	A	T	10: 77,157,626 (GRCm39)	F274I	probably damaging	Het
Aen	T	A	7: 78,552,107 (GRCm39)	D22E	probably damaging	Het
Appl1	T	C	14: 26,645,692 (GRCm39)	E706G	unknown	Het
Arhgap21	T	C	2: 20,860,469 (GRCm39)	T1313A	possibly damaging	Het
Armc2	T	C	10: 41,823,935 (GRCm39)	Y511C	probably damaging	Het
Bsn	T	C	9: 107,989,459 (GRCm39)	T2098A	probably damaging	Het
Cacna1h	T	C	17: 25,599,856 (GRCm39)	Y1659C	probably damaging	Het
Catsperb	T	C	12: 101,516,053 (GRCm39)	I563T	probably benign	Het
Cblif	G	A	19: 11,737,748 (GRCm39)	W386*	probably null	Het
Cd180	TA	TAA	13: 102,841,514 (GRCm39)		probably null	Het
Cd55b	A	T	1: 130,350,619 (GRCm39)	L26*	probably null	Het
Cdc42bpa	A	G	1: 179,933,638 (GRCm39)	N759S	probably benign	Het
Chrm3	C	A	13: 9,928,479 (GRCm39)	V186L	probably benign	Het
Clrn2	A	G	5: 45,621,283 (GRCm39)	T226A	probably damaging	Het
Col3a1	C	T	1: 45,383,138 (GRCm39)	P1071S	unknown	Het
Csgalnact2	A	G	6: 118,103,212 (GRCm39)	L250P	probably damaging	Het
Cyp4a31	G	T	4: 115,428,281 (GRCm39)	E326*	probably null	Het
Def8	A	G	8: 124,186,317 (GRCm39)	E352G	probably benign	Het
Dmbt1	A	G	7: 130,639,642 (GRCm39)	D60G	probably benign	Het
Dock3	A	G	9: 106,807,223 (GRCm39)	L1368P	probably damaging	Het
Dytn	A	T	1: 63,686,611 (GRCm39)	V353D	probably benign	Het
Egfem1	G	A	3: 29,411,317 (GRCm39)	E155K	probably benign	Het
Eif3m	A	T	2: 104,831,705 (GRCm39)	M285K	probably damaging	Het
Fam135b	T	C	15: 71,335,856 (GRCm39)	K446R	probably benign	Het
Fbxo22	T	C	9: 55,116,442 (GRCm39)	L36P	probably damaging	Het
Frmd4a	T	C	2: 4,612,844 (GRCm39)	S1025P	possibly damaging	Het
Gja8	C	T	3: 96,826,664 (GRCm39)	V333M	possibly damaging	Het
Glis1	T	C	4: 107,425,327 (GRCm39)	S313P	probably benign	Het
Grid2ip	G	A	5: 143,359,194 (GRCm39)	R270Q	probably damaging	Het
Hps4	G	A	5: 112,525,905 (GRCm39)	S642N	possibly damaging	Het
Hspa4	T	C	11: 53,171,466 (GRCm39)	E246G	probably benign	Het
Ibtk	A	G	9: 85,585,702 (GRCm39)		probably null	Het
Ifit1	A	G	19: 34,625,236 (GRCm39)	E124G	possibly damaging	Het
Ifna14	T	C	4: 88,489,752 (GRCm39)	D95G	probably benign	Het
Igsf10	G	A	3: 59,243,843 (GRCm39)	R164W	probably damaging	Het
Ilkap	A	C	1: 91,314,937 (GRCm39)	I142S	probably benign	Het
Insyn2a	A	T	7: 134,520,439 (GRCm39)	Y30*	probably null	Het
Kctd14	C	G	7: 97,104,104 (GRCm39)	P53R	unknown	Het
Kndc1	A	T	7: 139,500,600 (GRCm39)	D655V	probably damaging	Het
Krt1c	A	C	15: 101,725,948 (GRCm39)	S197A	probably benign	Het
Lpin1	A	T	12: 16,588,483 (GRCm39)	S902R	unknown	Het
Mapk13	T	C	17: 28,994,532 (GRCm39)	I141T	probably damaging	Het
Micall2	A	G	5: 139,701,827 (GRCm39)	V472A	unknown	Het
Mmp15	T	C	8: 96,092,959 (GRCm39)	F113L	probably benign	Het
Msantd5f6	C	T	4: 73,319,685 (GRCm39)	A264T	probably benign	Het
Msh2	T	G	17: 88,026,717 (GRCm39)	S738A	probably benign	Het
Muc20	T	G	16: 32,613,584 (GRCm39)	T598P	probably damaging	Het
Myo1f	T	C	17: 33,795,424 (GRCm39)	V53A	probably damaging	Het
Ncf1	A	T	5: 134,250,718 (GRCm39)	N367K	probably benign	Het
Nme8	T	G	13: 19,874,384 (GRCm39)	I139L	probably benign	Het
Odam	T	C	5: 88,034,457 (GRCm39)	F46L	probably benign	Het
Or7a42	G	A	10: 78,791,929 (GRCm39)	D297N	possibly damaging	Het
Pcare	A	G	17: 72,057,217 (GRCm39)	L820P	probably damaging	Het
Pi4ka	A	G	16: 17,099,788 (GRCm39)	I1945T		Het
Rbmyf9	T	G	Y: 3,774,819 (GRCm39)	D5E	probably damaging	Het
Rell1	T	C	5: 64,097,105 (GRCm39)		probably benign	Het
Ripk3	A	G	14: 56,023,303 (GRCm39)	F134S	probably benign	Het
Sclt1	A	T	3: 41,665,631 (GRCm39)	W146R	probably benign	Het
Slc18a2	T	C	19: 59,261,647 (GRCm39)	M178T	probably benign	Het
Slc24a5	G	T	2: 124,922,568 (GRCm39)	G110V	probably damaging	Het
Slc38a10	T	A	11: 119,996,781 (GRCm39)	D772V	probably benign	Het
Slc9c1	T	C	16: 45,398,275 (GRCm39)	L680P	possibly damaging	Het
Tll2	T	A	19: 41,093,436 (GRCm39)	Y477F	probably benign	Het
Tmco1	C	T	1: 167,136,132 (GRCm39)		probably benign	Het
Tnrc18	G	T	5: 142,773,392 (GRCm39)	A479D		Het
Topaz1	T	A	9: 122,596,097 (GRCm39)	M956K	probably benign	Het
Vmn2r56	T	A	7: 12,444,237 (GRCm39)	D465V	possibly damaging	Het
Xndc1	T	A	7: 101,722,476 (GRCm39)	V47E	probably damaging	Het

Other mutations in Or51a25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02832:Or51a25	APN	7	102,372,858 (GRCm39)	missense	probably benign	0.01
R0505:Or51a25	UTSW	7	102,373,236 (GRCm39)	missense	probably damaging	0.97
R1131:Or51a25	UTSW	7	102,372,887 (GRCm39)	missense	probably damaging	0.99
R1449:Or51a25	UTSW	7	102,373,397 (GRCm39)	missense	probably damaging	1.00
R3788:Or51a25	UTSW	7	102,372,694 (GRCm39)	splice site	probably null
R3915:Or51a25	UTSW	7	102,373,409 (GRCm39)	missense	possibly damaging	0.95
R3980:Or51a25	UTSW	7	102,372,959 (GRCm39)	missense	probably damaging	0.98
R4647:Or51a25	UTSW	7	102,373,299 (GRCm39)	missense	probably damaging	1.00
R5242:Or51a25	UTSW	7	102,373,483 (GRCm39)	missense	probably benign	0.41
R5404:Or51a25	UTSW	7	102,372,807 (GRCm39)	missense	possibly damaging	0.95
R5464:Or51a25	UTSW	7	102,373,124 (GRCm39)	missense	possibly damaging	0.85
R5807:Or51a25	UTSW	7	102,373,409 (GRCm39)	missense	possibly damaging	0.95
R5965:Or51a25	UTSW	7	102,373,467 (GRCm39)	missense	probably benign	0.00
R6008:Or51a25	UTSW	7	102,373,574 (GRCm39)	missense	probably damaging	1.00
R6036:Or51a25	UTSW	7	102,373,692 (GRCm39)	missense	probably benign
R6036:Or51a25	UTSW	7	102,373,692 (GRCm39)	missense	probably benign
R6493:Or51a25	UTSW	7	102,373,287 (GRCm39)	missense	possibly damaging	0.85
R6756:Or51a25	UTSW	7	102,373,295 (GRCm39)	missense	probably benign	0.03
R7201:Or51a25	UTSW	7	102,373,692 (GRCm39)	missense	probably benign
R7460:Or51a25	UTSW	7	102,373,028 (GRCm39)	missense	probably benign	0.09
R7695:Or51a25	UTSW	7	102,372,866 (GRCm39)	missense	probably benign	0.37
R8145:Or51a25	UTSW	7	102,372,937 (GRCm39)	missense	probably damaging	0.98
R8371:Or51a25	UTSW	7	102,372,790 (GRCm39)	missense	probably damaging	1.00
R8499:Or51a25	UTSW	7	102,372,932 (GRCm39)	missense	probably damaging	1.00
R9185:Or51a25	UTSW	7	102,373,409 (GRCm39)	missense	possibly damaging	0.95
X0025:Or51a25	UTSW	7	102,373,022 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTGTTGATACACACAACCAAGC -3'
(R):5'- GTAGACTCCTTGCTTATTGTCATG -3'

Sequencing Primer
(F):5'- CCAAGCAACTTGTATGACTTGATCC -3'
(R):5'- ATTCTTCACACAGTCATGGGGCTAG -3'

Posted On

2022-02-07