Incidental Mutation 'R9230:Mmp15'
ID 700175
Institutional Source Beutler Lab
Gene Symbol Mmp15
Ensembl Gene ENSMUSG00000031790
Gene Name matrix metallopeptidase 15
Synonyms MT2-MMP, Membrane type 2-MMP
MMRRC Submission
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R9230 (G1)
Quality Score 225.009
Status Validated
Chromosome 8
Chromosomal Location 96078924-96100921 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 96092959 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Leucine at position 113 (F113L)
Ref Sequence ENSEMBL: ENSMUSP00000034243 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000034243]
AlphaFold O54732
Predicted Effect probably benign
Transcript: ENSMUST00000034243
AA Change: F113L

PolyPhen 2 Score 0.061 (Sensitivity: 0.94; Specificity: 0.84)
SMART Domains Protein: ENSMUSP00000034243
Gene: ENSMUSG00000031790
AA Change: F113L

DomainStartEndE-ValueType
signal peptide 1 40 N/A INTRINSIC
Pfam:PG_binding_1 42 102 3.2e-13 PFAM
ZnMc 131 301 5.31e-59 SMART
low complexity region 306 353 N/A INTRINSIC
HX 370 413 5.92e-8 SMART
HX 415 459 2.31e-10 SMART
HX 462 508 2.98e-13 SMART
HX 510 555 2.01e-10 SMART
Pfam:DUF3377 586 657 1.2e-32 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.3%
Validation Efficiency 100% (68/68)
MGI Phenotype FUNCTION: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. A deficiency of the encoded protein in mice is compatible with normal development and postnatal growth. [provided by RefSeq, Feb 2016]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit normal phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adarb1 A T 10: 77,157,626 (GRCm39) F274I probably damaging Het
Aen T A 7: 78,552,107 (GRCm39) D22E probably damaging Het
Appl1 T C 14: 26,645,692 (GRCm39) E706G unknown Het
Arhgap21 T C 2: 20,860,469 (GRCm39) T1313A possibly damaging Het
Armc2 T C 10: 41,823,935 (GRCm39) Y511C probably damaging Het
Bsn T C 9: 107,989,459 (GRCm39) T2098A probably damaging Het
Cacna1h T C 17: 25,599,856 (GRCm39) Y1659C probably damaging Het
Catsperb T C 12: 101,516,053 (GRCm39) I563T probably benign Het
Cblif G A 19: 11,737,748 (GRCm39) W386* probably null Het
Cd180 TA TAA 13: 102,841,514 (GRCm39) probably null Het
Cd55b A T 1: 130,350,619 (GRCm39) L26* probably null Het
Cdc42bpa A G 1: 179,933,638 (GRCm39) N759S probably benign Het
Chrm3 C A 13: 9,928,479 (GRCm39) V186L probably benign Het
Clrn2 A G 5: 45,621,283 (GRCm39) T226A probably damaging Het
Col3a1 C T 1: 45,383,138 (GRCm39) P1071S unknown Het
Csgalnact2 A G 6: 118,103,212 (GRCm39) L250P probably damaging Het
Cyp4a31 G T 4: 115,428,281 (GRCm39) E326* probably null Het
Def8 A G 8: 124,186,317 (GRCm39) E352G probably benign Het
Dmbt1 A G 7: 130,639,642 (GRCm39) D60G probably benign Het
Dock3 A G 9: 106,807,223 (GRCm39) L1368P probably damaging Het
Dytn A T 1: 63,686,611 (GRCm39) V353D probably benign Het
Egfem1 G A 3: 29,411,317 (GRCm39) E155K probably benign Het
Eif3m A T 2: 104,831,705 (GRCm39) M285K probably damaging Het
Fam135b T C 15: 71,335,856 (GRCm39) K446R probably benign Het
Fbxo22 T C 9: 55,116,442 (GRCm39) L36P probably damaging Het
Frmd4a T C 2: 4,612,844 (GRCm39) S1025P possibly damaging Het
Gja8 C T 3: 96,826,664 (GRCm39) V333M possibly damaging Het
Glis1 T C 4: 107,425,327 (GRCm39) S313P probably benign Het
Grid2ip G A 5: 143,359,194 (GRCm39) R270Q probably damaging Het
Hps4 G A 5: 112,525,905 (GRCm39) S642N possibly damaging Het
Hspa4 T C 11: 53,171,466 (GRCm39) E246G probably benign Het
Ibtk A G 9: 85,585,702 (GRCm39) probably null Het
Ifit1 A G 19: 34,625,236 (GRCm39) E124G possibly damaging Het
Ifna14 T C 4: 88,489,752 (GRCm39) D95G probably benign Het
Igsf10 G A 3: 59,243,843 (GRCm39) R164W probably damaging Het
Ilkap A C 1: 91,314,937 (GRCm39) I142S probably benign Het
Insyn2a A T 7: 134,520,439 (GRCm39) Y30* probably null Het
Kctd14 C G 7: 97,104,104 (GRCm39) P53R unknown Het
Kndc1 A T 7: 139,500,600 (GRCm39) D655V probably damaging Het
Krt1c A C 15: 101,725,948 (GRCm39) S197A probably benign Het
Lpin1 A T 12: 16,588,483 (GRCm39) S902R unknown Het
Mapk13 T C 17: 28,994,532 (GRCm39) I141T probably damaging Het
Micall2 A G 5: 139,701,827 (GRCm39) V472A unknown Het
Msantd5f6 C T 4: 73,319,685 (GRCm39) A264T probably benign Het
Msh2 T G 17: 88,026,717 (GRCm39) S738A probably benign Het
Muc20 T G 16: 32,613,584 (GRCm39) T598P probably damaging Het
Myo1f T C 17: 33,795,424 (GRCm39) V53A probably damaging Het
Ncf1 A T 5: 134,250,718 (GRCm39) N367K probably benign Het
Nme8 T G 13: 19,874,384 (GRCm39) I139L probably benign Het
Odam T C 5: 88,034,457 (GRCm39) F46L probably benign Het
Or51a25 C A 7: 102,372,795 (GRCm39) V301F possibly damaging Het
Or7a42 G A 10: 78,791,929 (GRCm39) D297N possibly damaging Het
Pcare A G 17: 72,057,217 (GRCm39) L820P probably damaging Het
Pi4ka A G 16: 17,099,788 (GRCm39) I1945T Het
Rbmyf9 T G Y: 3,774,819 (GRCm39) D5E probably damaging Het
Rell1 T C 5: 64,097,105 (GRCm39) probably benign Het
Ripk3 A G 14: 56,023,303 (GRCm39) F134S probably benign Het
Sclt1 A T 3: 41,665,631 (GRCm39) W146R probably benign Het
Slc18a2 T C 19: 59,261,647 (GRCm39) M178T probably benign Het
Slc24a5 G T 2: 124,922,568 (GRCm39) G110V probably damaging Het
Slc38a10 T A 11: 119,996,781 (GRCm39) D772V probably benign Het
Slc9c1 T C 16: 45,398,275 (GRCm39) L680P possibly damaging Het
Tll2 T A 19: 41,093,436 (GRCm39) Y477F probably benign Het
Tmco1 C T 1: 167,136,132 (GRCm39) probably benign Het
Tnrc18 G T 5: 142,773,392 (GRCm39) A479D Het
Topaz1 T A 9: 122,596,097 (GRCm39) M956K probably benign Het
Vmn2r56 T A 7: 12,444,237 (GRCm39) D465V possibly damaging Het
Xndc1 T A 7: 101,722,476 (GRCm39) V47E probably damaging Het
Other mutations in Mmp15
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01467:Mmp15 APN 8 96,092,959 (GRCm39) missense probably benign 0.31
IGL03001:Mmp15 APN 8 96,094,845 (GRCm39) missense probably damaging 0.97
R0147:Mmp15 UTSW 8 96,098,945 (GRCm39) missense probably benign 0.18
R0148:Mmp15 UTSW 8 96,098,945 (GRCm39) missense probably benign 0.18
R0437:Mmp15 UTSW 8 96,097,400 (GRCm39) missense probably benign 0.04
R0465:Mmp15 UTSW 8 96,094,626 (GRCm39) missense probably damaging 1.00
R0548:Mmp15 UTSW 8 96,098,979 (GRCm39) missense probably damaging 1.00
R0574:Mmp15 UTSW 8 96,092,029 (GRCm39) missense possibly damaging 0.73
R0685:Mmp15 UTSW 8 96,098,762 (GRCm39) missense possibly damaging 0.81
R0763:Mmp15 UTSW 8 96,094,856 (GRCm39) missense probably benign 0.01
R1341:Mmp15 UTSW 8 96,098,931 (GRCm39) missense probably benign 0.03
R1428:Mmp15 UTSW 8 96,096,190 (GRCm39) missense probably benign 0.34
R1840:Mmp15 UTSW 8 96,092,048 (GRCm39) missense probably damaging 1.00
R2061:Mmp15 UTSW 8 96,097,407 (GRCm39) missense possibly damaging 0.91
R2219:Mmp15 UTSW 8 96,096,801 (GRCm39) missense probably benign 0.38
R4760:Mmp15 UTSW 8 96,094,824 (GRCm39) missense possibly damaging 0.61
R4762:Mmp15 UTSW 8 96,098,958 (GRCm39) missense probably benign 0.00
R5233:Mmp15 UTSW 8 96,097,696 (GRCm39) missense probably benign 0.08
R5394:Mmp15 UTSW 8 96,093,032 (GRCm39) missense probably damaging 0.96
R5502:Mmp15 UTSW 8 96,094,812 (GRCm39) missense possibly damaging 0.96
R5543:Mmp15 UTSW 8 96,094,729 (GRCm39) missense possibly damaging 0.85
R6027:Mmp15 UTSW 8 96,098,804 (GRCm39) missense probably benign 0.00
R6341:Mmp15 UTSW 8 96,092,091 (GRCm39) critical splice donor site probably null
R6720:Mmp15 UTSW 8 96,091,942 (GRCm39) missense probably benign 0.22
R7788:Mmp15 UTSW 8 96,094,776 (GRCm39) missense probably damaging 1.00
R8033:Mmp15 UTSW 8 96,094,590 (GRCm39) missense probably benign 0.01
R8679:Mmp15 UTSW 8 96,092,982 (GRCm39) missense possibly damaging 0.83
R8791:Mmp15 UTSW 8 96,096,288 (GRCm39) nonsense probably null
R9028:Mmp15 UTSW 8 96,096,316 (GRCm39) missense probably benign 0.01
R9227:Mmp15 UTSW 8 96,092,959 (GRCm39) missense probably benign 0.06
R9350:Mmp15 UTSW 8 96,093,002 (GRCm39) missense probably damaging 0.97
R9632:Mmp15 UTSW 8 96,098,731 (GRCm39) critical splice acceptor site probably null
R9695:Mmp15 UTSW 8 96,097,414 (GRCm39) missense possibly damaging 0.48
Predicted Primers PCR Primer
(F):5'- GAGTCACTCCTTAGCGTTCAATC -3'
(R):5'- AGTGACCCATCTTTCCCTGG -3'

Sequencing Primer
(F):5'- GCGTTCAATCTTCTTAAAATGGAATC -3'
(R):5'- CCCTAGATTGTGAGCTGGGAC -3'
Posted On 2022-02-07