Mutagenetix > Incidental Mutation

Incidental Mutation 'R9234:Wnk2'

701000

Institutional Source

Beutler Lab

Gene Symbol

Wnk2

Ensembl Gene

ENSMUSG00000037989

Gene Name

WNK lysine deficient protein kinase 2

Synonyms

X83337, ESTM15, 1810073P09Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.287) question?

Stock #

R9234 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

49189779-49301490 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 49224274 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 1171 (D1171G)

Ref Sequence

ENSEMBL: ENSMUSP00000047231 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035538] [ENSMUST00000049265] [ENSMUST00000091623] [ENSMUST00000110096] [ENSMUST00000110097] [ENSMUST00000159559] [ENSMUST00000160087] [ENSMUST00000162403] [ENSMUST00000162581]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000035538
AA Change: D1171G

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000047231
Gene: ENSMUSG00000037989
AA Change: D1171G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000049265
AA Change: D1171G

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049327
Gene: ENSMUSG00000037989
AA Change: D1171G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
S_TKc	195	453	3.3e-19	SMART
Pfam:OSR1_C	474	511	5.6e-19	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	9.51e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1703	2.12e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2023	2.12e-5	PROSPERO
low complexity region	2069	2089	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000091623
AA Change: D1171G

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000089212
Gene: ENSMUSG00000037989
AA Change: D1171G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	1.6e-41	PFAM
Pfam:Pkinase	195	453	1e-54	PFAM
Pfam:OSR1_C	474	511	4e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.52e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	7.41e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	7.41e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC
low complexity region	2191	2202	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110096
AA Change: D1073G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000105723
Gene: ENSMUSG00000037989
AA Change: D1073G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.6e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	1015	1038	N/A	INTRINSIC
internal_repeat_1	1064	1087	2.89e-5	PROSPERO
low complexity region	1177	1194	N/A	INTRINSIC
low complexity region	1219	1255	N/A	INTRINSIC
low complexity region	1331	1343	N/A	INTRINSIC
low complexity region	1351	1359	N/A	INTRINSIC
low complexity region	1366	1391	N/A	INTRINSIC
low complexity region	1489	1498	N/A	INTRINSIC
low complexity region	1721	1732	N/A	INTRINSIC
coiled coil region	1737	1768	N/A	INTRINSIC
low complexity region	2018	2038	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000110097
AA Change: D1171G

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000105724
Gene: ENSMUSG00000037989
AA Change: D1171G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	1.16e-5	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
internal_repeat_2	1611	1713	5.74e-5	PROSPERO
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
internal_repeat_2	1923	2027	5.74e-5	PROSPERO
low complexity region	2117	2137	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000159559
AA Change: D1159G

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000123915
Gene: ENSMUSG00000037989
AA Change: D1159G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.7e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.8e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	971	1002	N/A	INTRINSIC
low complexity region	1009	1036	N/A	INTRINSIC
low complexity region	1101	1124	N/A	INTRINSIC
internal_repeat_1	1150	1173	7.19e-6	PROSPERO
low complexity region	1263	1280	N/A	INTRINSIC
low complexity region	1305	1341	N/A	INTRINSIC
low complexity region	1417	1429	N/A	INTRINSIC
low complexity region	1437	1445	N/A	INTRINSIC
low complexity region	1452	1477	N/A	INTRINSIC
low complexity region	1575	1585	N/A	INTRINSIC
internal_repeat_2	1599	1701	3.66e-5	PROSPERO
low complexity region	1808	1819	N/A	INTRINSIC
coiled coil region	1824	1855	N/A	INTRINSIC
internal_repeat_2	1911	2015	3.66e-5	PROSPERO
low complexity region	2105	2125	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000159633
AA Change: D50G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000160087
AA Change: D313G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000124614
Gene: ENSMUSG00000037989
AA Change: D313G

Domain	Start	End	E-Value	Type
low complexity region	61	71	N/A	INTRINSIC
low complexity region	80	94	N/A	INTRINSIC
low complexity region	115	130	N/A	INTRINSIC
low complexity region	178	194	N/A	INTRINSIC
low complexity region	255	278	N/A	INTRINSIC
low complexity region	417	434	N/A	INTRINSIC
low complexity region	459	495	N/A	INTRINSIC
low complexity region	571	583	N/A	INTRINSIC
low complexity region	591	599	N/A	INTRINSIC
low complexity region	606	631	N/A	INTRINSIC
low complexity region	729	738	N/A	INTRINSIC
low complexity region	961	972	N/A	INTRINSIC
coiled coil region	977	1008	N/A	INTRINSIC
low complexity region	1258	1278	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000162403
AA Change: D1061G

PolyPhen 2 Score 0.682 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000125539
Gene: ENSMUSG00000037989
AA Change: D1061G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.3e-42	PFAM
Pfam:Pkinase	195	453	1.6e-56	PFAM
Pfam:OSR1_C	474	511	5.3e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	736	N/A	INTRINSIC
low complexity region	759	791	N/A	INTRINSIC
low complexity region	809	819	N/A	INTRINSIC
low complexity region	828	842	N/A	INTRINSIC
low complexity region	863	878	N/A	INTRINSIC
low complexity region	926	942	N/A	INTRINSIC
low complexity region	1003	1026	N/A	INTRINSIC
internal_repeat_1	1052	1075	1.05e-5	PROSPERO
low complexity region	1165	1182	N/A	INTRINSIC
low complexity region	1207	1243	N/A	INTRINSIC
low complexity region	1319	1331	N/A	INTRINSIC
low complexity region	1339	1347	N/A	INTRINSIC
low complexity region	1354	1379	N/A	INTRINSIC
low complexity region	1477	1487	N/A	INTRINSIC
internal_repeat_2	1501	1593	2.32e-5	PROSPERO
low complexity region	1710	1721	N/A	INTRINSIC
coiled coil region	1726	1757	N/A	INTRINSIC
internal_repeat_2	1813	1913	2.32e-5	PROSPERO
low complexity region	1959	1979	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000162581
AA Change: D1171G

PolyPhen 2 Score 0.985 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000125448
Gene: ENSMUSG00000037989
AA Change: D1171G

Domain	Start	End	E-Value	Type
low complexity region	2	9	N/A	INTRINSIC
low complexity region	66	78	N/A	INTRINSIC
low complexity region	88	116	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
low complexity region	171	182	N/A	INTRINSIC
Pfam:Pkinase_Tyr	195	451	4.5e-42	PFAM
Pfam:Pkinase	195	453	1.7e-56	PFAM
Pfam:OSR1_C	474	511	5.6e-22	PFAM
low complexity region	582	593	N/A	INTRINSIC
low complexity region	626	642	N/A	INTRINSIC
low complexity region	678	693	N/A	INTRINSIC
low complexity region	707	724	N/A	INTRINSIC
low complexity region	771	803	N/A	INTRINSIC
low complexity region	821	831	N/A	INTRINSIC
low complexity region	840	854	N/A	INTRINSIC
low complexity region	875	890	N/A	INTRINSIC
low complexity region	938	954	N/A	INTRINSIC
low complexity region	983	1014	N/A	INTRINSIC
low complexity region	1021	1048	N/A	INTRINSIC
low complexity region	1113	1136	N/A	INTRINSIC
internal_repeat_1	1162	1185	6.72e-6	PROSPERO
low complexity region	1275	1292	N/A	INTRINSIC
low complexity region	1317	1353	N/A	INTRINSIC
low complexity region	1429	1441	N/A	INTRINSIC
low complexity region	1449	1457	N/A	INTRINSIC
low complexity region	1464	1489	N/A	INTRINSIC
low complexity region	1587	1597	N/A	INTRINSIC
low complexity region	1820	1831	N/A	INTRINSIC
coiled coil region	1836	1867	N/A	INTRINSIC
low complexity region	2033	2053	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic serine-threonine kinase that belongs to the protein kinase superfamily. The protein plays an important role in the regulation of electrolyte homeostasis, cell signaling survival, and proliferation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430550D23Rik	A	G	2: 155,845,798 (GRCm39)	Y20H	probably benign	Het
Arid5b	A	G	10: 67,964,628 (GRCm39)	F348L	possibly damaging	Het
Bms1	C	A	6: 118,375,044 (GRCm39)	A777S	probably damaging	Het
C2cd3	A	G	7: 100,049,012 (GRCm39)	Y61C		Het
Caprin2	A	T	6: 148,744,337 (GRCm39)	Y1029*	probably null	Het
Casp1	A	T	9: 5,303,128 (GRCm39)	D194V	probably benign	Het
Crkl	A	G	16: 17,286,822 (GRCm39)	E126G	probably damaging	Het
Crp	A	T	1: 172,526,413 (GRCm39)	D166V	probably damaging	Het
Dnah10	A	C	5: 124,818,989 (GRCm39)	D425A	possibly damaging	Het
Dnah11	A	T	12: 117,951,095 (GRCm39)	L3071Q	probably damaging	Het
Dnah9	T	A	11: 65,924,751 (GRCm39)	I2168F	possibly damaging	Het
Elp3	T	A	14: 65,788,920 (GRCm39)	I388F	probably damaging	Het
Espn	C	A	4: 152,217,380 (GRCm39)		probably null	Het
Fbrsl1	G	T	5: 110,511,250 (GRCm39)	F504L	probably benign	Het
Fzd9	A	C	5: 135,279,540 (GRCm39)	M115R	probably damaging	Het
Git2	A	T	5: 114,899,682 (GRCm39)	Y224N	possibly damaging	Het
Gm9195	A	T	14: 72,695,786 (GRCm39)	Y1415*	probably null	Het
Gnpat	T	A	8: 125,610,179 (GRCm39)	L454Q	probably damaging	Het
Grm5	C	T	7: 87,723,440 (GRCm39)	P577S	probably damaging	Het
Hfm1	T	C	5: 107,041,334 (GRCm39)	N683S	probably benign	Het
Hps4	G	A	5: 112,525,905 (GRCm39)	S642N	possibly damaging	Het
Leng8	C	A	7: 4,145,247 (GRCm39)	P216T	probably damaging	Het
Letm2	C	A	8: 26,084,102 (GRCm39)	R43L	probably benign	Het
Lims2	A	T	18: 32,090,943 (GRCm39)	Y309F	probably benign	Het
Llgl1	T	C	11: 60,600,956 (GRCm39)		probably null	Het
Ltn1	A	T	16: 87,194,089 (GRCm39)	D1417E	probably damaging	Het
Map4k4	T	A	1: 40,029,261 (GRCm39)	N345K	unknown	Het
Mapre2	T	C	18: 23,937,236 (GRCm39)	I26T	probably benign	Het
Nfe2	A	G	15: 103,159,636 (GRCm39)		probably benign	Het
Nlgn3	T	C	X: 100,352,390 (GRCm39)	V179A	probably damaging	Het
Ntrk1	A	G	3: 87,695,622 (GRCm39)		probably null	Het
Or51b6b	T	A	7: 103,309,950 (GRCm39)	Y169F	possibly damaging	Het
Or52e18	A	G	7: 104,609,651 (GRCm39)	F96S	probably damaging	Het
Or5h19	T	C	16: 58,856,789 (GRCm39)	N104D	probably benign	Het
Otud7b	G	T	3: 96,047,771 (GRCm39)	Q43H	probably damaging	Het
Pcdhb5	T	C	18: 37,453,695 (GRCm39)	V25A	probably benign	Het
Pcolce2	A	G	9: 95,560,439 (GRCm39)	T163A	probably benign	Het
Pdgfra	T	A	5: 75,324,262 (GRCm39)	V39E	possibly damaging	Het
Pdgfrb	T	A	18: 61,194,300 (GRCm39)	W26R	probably null	Het
Pigp	T	A	16: 94,165,522 (GRCm39)	*206Y	probably null	Het
Pip5kl1	G	T	2: 32,468,211 (GRCm39)	G126C	probably benign	Het
Plekhg2	G	T	7: 28,064,215 (GRCm39)	T442K	probably benign	Het
Prmt5	A	T	14: 54,748,674 (GRCm39)	S375T	possibly damaging	Het
Rnf213	T	A	11: 119,340,943 (GRCm39)	M3529K		Het
Ror2	C	A	13: 53,265,374 (GRCm39)	D573Y	probably damaging	Het
Shank1	A	G	7: 43,962,579 (GRCm39)	T98A	unknown	Het
Shcbp1	A	T	8: 4,798,800 (GRCm39)	I373K	possibly damaging	Het
Slc25a42	A	G	8: 70,642,736 (GRCm39)	S64P	probably damaging	Het
Smco3	T	A	6: 136,808,410 (GRCm39)	I155F	probably damaging	Het
Snx4	C	A	16: 33,087,161 (GRCm39)	S102*	probably null	Het
Snx4	T	A	16: 33,108,069 (GRCm39)	S288T	probably benign	Het
Socs4	A	G	14: 47,527,716 (GRCm39)	N217S	probably benign	Het
Tatdn2	G	A	6: 113,679,683 (GRCm39)		probably null	Het
Tmprss2	A	G	16: 97,379,821 (GRCm39)	Y160H	probably damaging	Het
Trim37	T	C	11: 87,036,393 (GRCm39)	I158T	possibly damaging	Het
Trip11	C	T	12: 101,811,990 (GRCm39)		probably null	Het
Ubr3	G	A	2: 69,727,990 (GRCm39)	A118T	probably benign	Het
Ubxn7	T	C	16: 32,178,895 (GRCm39)		probably null	Het
Unc5cl	C	T	17: 48,770,658 (GRCm39)	H369Y	probably damaging	Het
Unc5d	A	T	8: 29,250,877 (GRCm39)	N263K	probably damaging	Het
Vmn2r59	A	G	7: 41,661,907 (GRCm39)	I636T	possibly damaging	Het
Vps13d	G	T	4: 144,875,792 (GRCm39)	Q1578K		Het
Zfpm2	A	G	15: 40,966,470 (GRCm39)	Y985C	probably damaging	Het
Zpbp2	A	T	11: 98,443,398 (GRCm39)	N77I	probably damaging	Het

Other mutations in Wnk2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Wnk2	APN	13	49,221,668 (GRCm39)	missense	possibly damaging	0.89
IGL01575:Wnk2	APN	13	49,300,152 (GRCm39)	missense	probably damaging	1.00
IGL01601:Wnk2	APN	13	49,230,038 (GRCm39)	missense	probably damaging	1.00
IGL01775:Wnk2	APN	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
IGL02013:Wnk2	APN	13	49,235,510 (GRCm39)	missense	possibly damaging	0.46
IGL02016:Wnk2	APN	13	49,210,381 (GRCm39)	missense	probably damaging	1.00
IGL02167:Wnk2	APN	13	49,224,601 (GRCm39)	critical splice acceptor site	probably null
IGL02174:Wnk2	APN	13	49,210,643 (GRCm39)	missense	probably damaging	1.00
IGL02210:Wnk2	APN	13	49,244,345 (GRCm39)	missense	probably damaging	0.98
IGL02228:Wnk2	APN	13	49,210,416 (GRCm39)	missense	probably damaging	1.00
IGL02282:Wnk2	APN	13	49,221,601 (GRCm39)	missense	probably damaging	1.00
IGL02319:Wnk2	APN	13	49,214,914 (GRCm39)	missense	possibly damaging	0.73
IGL02394:Wnk2	APN	13	49,235,375 (GRCm39)	splice site	probably null
IGL02624:Wnk2	APN	13	49,256,278 (GRCm39)	missense	probably damaging	1.00
IGL02743:Wnk2	APN	13	49,248,920 (GRCm39)	missense	probably damaging	1.00
IGL03012:Wnk2	APN	13	49,197,865 (GRCm39)	missense	probably damaging	0.99
IGL03166:Wnk2	APN	13	49,224,520 (GRCm39)	nonsense	probably null
R0034:Wnk2	UTSW	13	49,221,556 (GRCm39)	missense	possibly damaging	0.64
R0385:Wnk2	UTSW	13	49,221,604 (GRCm39)	missense	probably damaging	1.00
R0423:Wnk2	UTSW	13	49,248,894 (GRCm39)	missense	possibly damaging	0.91
R0504:Wnk2	UTSW	13	49,238,872 (GRCm39)	missense	probably damaging	1.00
R0504:Wnk2	UTSW	13	49,238,870 (GRCm39)	missense	possibly damaging	0.92
R0653:Wnk2	UTSW	13	49,210,492 (GRCm39)	missense	possibly damaging	0.85
R1135:Wnk2	UTSW	13	49,230,034 (GRCm39)	missense	probably damaging	1.00
R1445:Wnk2	UTSW	13	49,224,586 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1464:Wnk2	UTSW	13	49,235,451 (GRCm39)	missense	probably damaging	1.00
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1468:Wnk2	UTSW	13	49,235,571 (GRCm39)	missense	probably damaging	0.99
R1480:Wnk2	UTSW	13	49,210,708 (GRCm39)	missense	probably damaging	1.00
R1605:Wnk2	UTSW	13	49,214,370 (GRCm39)	missense	probably damaging	1.00
R1719:Wnk2	UTSW	13	49,214,202 (GRCm39)	missense	possibly damaging	0.76
R1891:Wnk2	UTSW	13	49,206,200 (GRCm39)	nonsense	probably null
R1966:Wnk2	UTSW	13	49,192,487 (GRCm39)	missense	probably damaging	0.96
R2001:Wnk2	UTSW	13	49,232,158 (GRCm39)	missense	possibly damaging	0.61
R2310:Wnk2	UTSW	13	49,204,053 (GRCm39)	missense	probably damaging	0.97
R2356:Wnk2	UTSW	13	49,192,644 (GRCm39)	nonsense	probably null
R2406:Wnk2	UTSW	13	49,214,964 (GRCm39)	missense	possibly damaging	0.86
R2519:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably damaging	0.99
R3962:Wnk2	UTSW	13	49,224,453 (GRCm39)	missense	probably damaging	1.00
R4160:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4161:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4226:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4227:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4234:Wnk2	UTSW	13	49,214,604 (GRCm39)	missense	probably benign	0.33
R4304:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4308:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4584:Wnk2	UTSW	13	49,244,313 (GRCm39)	missense	probably damaging	1.00
R4655:Wnk2	UTSW	13	49,210,359 (GRCm39)	missense	probably damaging	1.00
R4715:Wnk2	UTSW	13	49,300,708 (GRCm39)	start codon destroyed	unknown
R4887:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4888:Wnk2	UTSW	13	49,224,478 (GRCm39)	missense	probably damaging	1.00
R4945:Wnk2	UTSW	13	49,210,722 (GRCm39)	missense	probably damaging	1.00
R5182:Wnk2	UTSW	13	49,214,637 (GRCm39)	missense	possibly damaging	0.92
R5243:Wnk2	UTSW	13	49,226,054 (GRCm39)	missense	possibly damaging	0.51
R5370:Wnk2	UTSW	13	49,256,437 (GRCm39)	missense	probably damaging	1.00
R5771:Wnk2	UTSW	13	49,256,276 (GRCm39)	missense	probably damaging	1.00
R5877:Wnk2	UTSW	13	49,220,782 (GRCm39)	missense	probably damaging	0.98
R5900:Wnk2	UTSW	13	49,256,308 (GRCm39)	missense	probably damaging	1.00
R5905:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R5912:Wnk2	UTSW	13	49,214,250 (GRCm39)	missense	probably damaging	1.00
R5915:Wnk2	UTSW	13	49,231,561 (GRCm39)	missense	probably damaging	0.99
R6028:Wnk2	UTSW	13	49,229,821 (GRCm39)	missense	probably damaging	0.99
R6074:Wnk2	UTSW	13	49,204,875 (GRCm39)	missense	probably damaging	1.00
R6171:Wnk2	UTSW	13	49,214,308 (GRCm39)	missense	probably damaging	1.00
R6368:Wnk2	UTSW	13	49,214,814 (GRCm39)	missense	probably damaging	0.99
R6467:Wnk2	UTSW	13	49,300,081 (GRCm39)	missense	probably damaging	1.00
R6501:Wnk2	UTSW	13	49,300,159 (GRCm39)	missense	probably damaging	1.00
R6849:Wnk2	UTSW	13	49,220,834 (GRCm39)	missense	probably damaging	1.00
R6898:Wnk2	UTSW	13	49,224,557 (GRCm39)	missense	probably damaging	1.00
R6949:Wnk2	UTSW	13	49,254,616 (GRCm39)	missense	probably damaging	1.00
R7011:Wnk2	UTSW	13	49,224,567 (GRCm39)	missense	probably damaging	0.99
R7097:Wnk2	UTSW	13	49,256,314 (GRCm39)	missense	possibly damaging	0.86
R7121:Wnk2	UTSW	13	49,300,653 (GRCm39)	missense	probably benign	0.26
R7123:Wnk2	UTSW	13	49,235,462 (GRCm39)	missense	possibly damaging	0.90
R7423:Wnk2	UTSW	13	49,191,608 (GRCm39)	missense	probably benign	0.07
R7502:Wnk2	UTSW	13	49,300,720 (GRCm39)	splice site	probably null
R7529:Wnk2	UTSW	13	49,254,457 (GRCm39)	missense	possibly damaging	0.50
R7751:Wnk2	UTSW	13	49,231,493 (GRCm39)	missense	unknown
R7979:Wnk2	UTSW	13	49,248,884 (GRCm39)	missense	probably damaging	1.00
R8118:Wnk2	UTSW	13	49,244,459 (GRCm39)	missense	probably damaging	0.99
R8121:Wnk2	UTSW	13	49,214,415 (GRCm39)	nonsense	probably null
R8155:Wnk2	UTSW	13	49,192,577 (GRCm39)	missense	unknown
R8329:Wnk2	UTSW	13	49,248,914 (GRCm39)	missense	probably damaging	1.00
R8334:Wnk2	UTSW	13	49,203,958 (GRCm39)	critical splice donor site	probably null
R8872:Wnk2	UTSW	13	49,210,960 (GRCm39)	missense	probably benign	0.00
R8919:Wnk2	UTSW	13	49,221,711 (GRCm39)	missense	possibly damaging	0.86
R9091:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9262:Wnk2	UTSW	13	49,221,430 (GRCm39)	missense	probably benign	0.12
R9268:Wnk2	UTSW	13	49,235,507 (GRCm39)	missense	possibly damaging	0.82
R9270:Wnk2	UTSW	13	49,224,505 (GRCm39)	missense	probably benign	0.41
R9386:Wnk2	UTSW	13	49,220,822 (GRCm39)	missense	probably damaging	0.98
R9582:Wnk2	UTSW	13	49,210,975 (GRCm39)	missense	probably benign	0.01
R9617:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
R9625:Wnk2	UTSW	13	49,254,445 (GRCm39)	missense	probably benign	0.20
R9794:Wnk2	UTSW	13	49,229,674 (GRCm39)	missense	probably benign	0.02
RF023:Wnk2	UTSW	13	49,300,255 (GRCm39)	missense	probably benign	0.00
X0025:Wnk2	UTSW	13	49,214,418 (GRCm39)	missense	probably damaging	0.99
X0063:Wnk2	UTSW	13	49,192,453 (GRCm39)	missense	unknown
Z1176:Wnk2	UTSW	13	49,191,537 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TTACTAAGGAGTCTGGGGAAAACC -3'
(R):5'- CCGGCTCACTATCCTAAATGTGAG -3'

Sequencing Primer
(F):5'- TCTGCAGGAAGCTGATGC -3'
(R):5'- CACTATCCTAAATGTGAGTGGCTGC -3'

Posted On

2022-03-25