Incidental Mutation 'R9255:Vmn2r-ps158'
ID 701804
Institutional Source Beutler Lab
Gene Symbol Vmn2r-ps158
Ensembl Gene ENSMUSG00000091528
Gene Name vomeronasal 2, receptor, pseudogene 158
Synonyms Vmn2r126, Gm9268
MMRRC Submission 068961-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.093) question?
Stock # R9255 (G1)
Quality Score 135.008
Status Not validated
Chromosome 7
Chromosomal Location 42668222-42697530 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 42673965 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Phenylalanine at position 348 (Y348F)
Ref Sequence ENSEMBL: ENSMUSP00000129068 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000166914] [ENSMUST00000173815]
AlphaFold E9Q0M3
Predicted Effect possibly damaging
Transcript: ENSMUST00000166914
AA Change: Y348F

PolyPhen 2 Score 0.753 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000129068
Gene: ENSMUSG00000091528
AA Change: Y348F

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
Pfam:ANF_receptor 77 473 1.1e-41 PFAM
Pfam:NCD3G 516 569 1.7e-23 PFAM
Pfam:7tm_3 602 837 9.6e-51 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000173815
AA Change: Y341F

PolyPhen 2 Score 0.958 (Sensitivity: 0.78; Specificity: 0.95)
SMART Domains Protein: ENSMUSP00000134441
Gene: ENSMUSG00000091528
AA Change: Y341F

DomainStartEndE-ValueType
signal peptide 1 18 N/A INTRINSIC
Pfam:ANF_receptor 77 467 5.3e-40 PFAM
Pfam:NCD3G 509 562 4.6e-22 PFAM
Pfam:7tm_3 594 831 1.1e-73 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.2%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 71 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A2m A G 6: 121,626,795 (GRCm39) Y549C probably damaging Het
Abca13 A C 11: 9,278,213 (GRCm39) N3131T probably damaging Het
Acap3 T C 4: 155,990,145 (GRCm39) V775A probably benign Het
Actn3 T A 19: 4,921,820 (GRCm39) I149F probably damaging Het
Adcy2 A T 13: 69,036,199 (GRCm39) M154K possibly damaging Het
Agbl1 A T 7: 76,416,150 (GRCm39) M976L unknown Het
Agbl3 T A 6: 34,789,840 (GRCm39) M531K probably damaging Het
Armc8 A C 9: 99,379,441 (GRCm39) M495R possibly damaging Het
Astn2 T A 4: 65,563,085 (GRCm39) K899* probably null Het
Atp10b T A 11: 43,107,148 (GRCm39) H727Q probably damaging Het
Bach1 A G 16: 87,519,401 (GRCm39) I564V possibly damaging Het
Bean1 CT C 8: 104,908,664 (GRCm39) probably null Het
Ccdc136 T A 6: 29,409,237 (GRCm39) H183Q probably benign Het
Celf3 T C 3: 94,392,594 (GRCm39) F98L probably benign Het
Chrd G A 16: 20,558,801 (GRCm39) C845Y probably damaging Het
Clec1a T G 6: 129,409,208 (GRCm39) K139N probably benign Het
Cntln T G 4: 85,019,103 (GRCm39) N130K possibly damaging Het
Cog1 A T 11: 113,547,019 (GRCm39) T538S probably benign Het
Cog8 A G 8: 107,779,383 (GRCm39) I298T probably damaging Het
Dio1 A T 4: 107,164,102 (GRCm39) W72R probably damaging Het
Elapor1 T C 3: 108,376,864 (GRCm39) R453G probably damaging Het
Exph5 T A 9: 53,284,609 (GRCm39) N563K possibly damaging Het
Foxn1 C A 11: 78,252,399 (GRCm39) E330* probably null Het
Fzd7 G T 1: 59,522,495 (GRCm39) R126L possibly damaging Het
Gmnn A G 13: 24,937,351 (GRCm39) Y147H probably benign Het
Gpa33 A G 1: 165,980,186 (GRCm39) N83S probably benign Het
Hdac4 A G 1: 91,889,173 (GRCm39) probably null Het
Hydin A G 8: 111,261,972 (GRCm39) D2691G probably benign Het
Ifi203 A T 1: 173,756,787 (GRCm39) I332N unknown Het
Inpp1 A G 1: 52,829,306 (GRCm39) S238P probably benign Het
Jmy A G 13: 93,589,894 (GRCm39) probably null Het
Kcnab3 C T 11: 69,222,337 (GRCm39) Q327* probably null Het
Kctd1 A G 18: 15,194,853 (GRCm39) V590A probably benign Het
Lrrc8e A T 8: 4,284,504 (GRCm39) H243L probably damaging Het
Lsr A G 7: 30,657,670 (GRCm39) L498S probably benign Het
Mapkbp1 A G 2: 119,843,556 (GRCm39) D268G probably damaging Het
Mfn1 T C 3: 32,598,287 (GRCm39) V203A possibly damaging Het
Micu1 T A 10: 59,604,051 (GRCm39) I212N probably damaging Het
Nicn1 C T 9: 108,171,708 (GRCm39) R163C possibly damaging Het
Nos1 G C 5: 118,017,402 (GRCm39) R255P probably benign Het
Or11i1 A G 3: 106,729,737 (GRCm39) I46T probably benign Het
Or12j4 T C 7: 140,046,423 (GRCm39) F103S probably damaging Het
Or8g30 T C 9: 39,230,487 (GRCm39) Y141C probably benign Het
Pabpc6 T C 17: 9,886,769 (GRCm39) E594G probably damaging Het
Pald1 A T 10: 61,176,989 (GRCm39) probably null Het
Pdpk1 A G 17: 24,325,938 (GRCm39) I113T possibly damaging Het
Perm1 TGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCT TGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCT 4: 156,302,525 (GRCm39) probably benign Het
Pi4kb T C 3: 94,906,219 (GRCm39) S589P probably damaging Het
Pik3ca G A 3: 32,496,981 (GRCm39) probably null Het
Plekhg4 A T 8: 106,103,271 (GRCm39) M315L probably benign Het
Proz G A 8: 13,123,472 (GRCm39) V248M possibly damaging Het
Prss1 T C 6: 41,438,183 (GRCm39) Y37H probably damaging Het
R3hdml T A 2: 163,337,612 (GRCm39) W89R probably damaging Het
Ranbp3l A G 15: 9,057,293 (GRCm39) T319A probably benign Het
Scn4a C T 11: 106,215,054 (GRCm39) A1246T probably damaging Het
Sdr42e1 T A 8: 118,389,999 (GRCm39) Y214F probably benign Het
Slc10a2 C T 8: 5,148,565 (GRCm39) V127I probably benign Het
Slc16a1 T A 3: 104,560,148 (GRCm39) M151K possibly damaging Het
Slc25a25 C T 2: 32,310,391 (GRCm39) E123K probably damaging Het
Ssbp2 T A 13: 91,818,525 (GRCm39) M171K possibly damaging Het
Styk1 A G 6: 131,286,946 (GRCm39) V183A probably damaging Het
Tmco1 C T 1: 167,136,132 (GRCm39) probably benign Het
Tspan18 T G 2: 93,040,200 (GRCm39) D185A probably benign Het
Tti2 T A 8: 31,645,570 (GRCm39) F320Y probably damaging Het
Ttn T C 2: 76,608,621 (GRCm39) I17798M probably damaging Het
Ube2j1 C A 4: 33,036,759 (GRCm39) H28N probably benign Het
Vmn1r195 A C 13: 22,463,342 (GRCm39) I271L possibly damaging Het
Vmn2r81 A C 10: 79,103,166 (GRCm39) T120P possibly damaging Het
Wtip A G 7: 33,824,908 (GRCm39) probably null Het
Zc3h12d C T 10: 7,729,022 (GRCm39) H129Y probably damaging Het
Zfp600 T G 4: 146,131,673 (GRCm39) S114A possibly damaging Het
Other mutations in Vmn2r-ps158
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01676:Vmn2r-ps158 APN 7 42,674,133 (GRCm39) missense probably damaging 0.97
IGL01753:Vmn2r-ps158 APN 7 42,674,139 (GRCm39) missense probably damaging 1.00
IGL02338:Vmn2r-ps158 APN 7 42,697,160 (GRCm39) missense probably damaging 1.00
IGL02541:Vmn2r-ps158 APN 7 42,673,092 (GRCm39) splice site probably benign
R0751:Vmn2r-ps158 UTSW 7 42,696,833 (GRCm39) missense probably damaging 0.99
R0849:Vmn2r-ps158 UTSW 7 42,674,142 (GRCm39) missense probably damaging 1.00
R1460:Vmn2r-ps158 UTSW 7 42,672,639 (GRCm39) missense probably benign 0.25
R1617:Vmn2r-ps158 UTSW 7 42,673,503 (GRCm39) missense probably benign 0.18
R1962:Vmn2r-ps158 UTSW 7 42,696,824 (GRCm39) missense probably benign 0.00
R1999:Vmn2r-ps158 UTSW 7 42,696,883 (GRCm39) missense probably damaging 1.00
R2022:Vmn2r-ps158 UTSW 7 42,673,454 (GRCm39) missense probably benign
R2434:Vmn2r-ps158 UTSW 7 42,696,881 (GRCm39) missense probably damaging 0.99
R3760:Vmn2r-ps158 UTSW 7 42,673,502 (GRCm39) missense probably benign 0.00
R4562:Vmn2r-ps158 UTSW 7 42,672,986 (GRCm39) nonsense probably null
R4890:Vmn2r-ps158 UTSW 7 42,697,024 (GRCm39) missense probably damaging 1.00
R5221:Vmn2r-ps158 UTSW 7 42,672,684 (GRCm39) missense probably benign 0.31
R5597:Vmn2r-ps158 UTSW 7 42,674,073 (GRCm39) missense probably benign 0.01
R6589:Vmn2r-ps158 UTSW 7 42,673,022 (GRCm39) missense possibly damaging 0.91
R6831:Vmn2r-ps158 UTSW 7 42,673,004 (GRCm39) missense probably damaging 1.00
R6834:Vmn2r-ps158 UTSW 7 42,673,004 (GRCm39) missense probably damaging 1.00
R6910:Vmn2r-ps158 UTSW 7 42,673,475 (GRCm39) missense probably benign 0.01
R6944:Vmn2r-ps158 UTSW 7 42,697,393 (GRCm39) missense possibly damaging 0.95
R7470:Vmn2r-ps158 UTSW 7 42,697,310 (GRCm39) missense probably damaging 1.00
R7553:Vmn2r-ps158 UTSW 7 42,697,447 (GRCm39) missense probably damaging 1.00
R7661:Vmn2r-ps158 UTSW 7 42,672,582 (GRCm39) missense probably benign 0.00
R7677:Vmn2r-ps158 UTSW 7 42,674,163 (GRCm39) missense probably damaging 1.00
R8087:Vmn2r-ps158 UTSW 7 42,697,094 (GRCm39) missense probably benign 0.04
R8210:Vmn2r-ps158 UTSW 7 42,673,462 (GRCm39) missense probably benign 0.16
R8379:Vmn2r-ps158 UTSW 7 42,697,270 (GRCm39) missense probably damaging 1.00
R8692:Vmn2r-ps158 UTSW 7 42,697,108 (GRCm39) missense probably benign 0.13
R8917:Vmn2r-ps158 UTSW 7 42,697,433 (GRCm39) missense probably damaging 1.00
R9375:Vmn2r-ps158 UTSW 7 42,673,499 (GRCm39) missense possibly damaging 0.87
R9452:Vmn2r-ps158 UTSW 7 42,697,257 (GRCm39) missense possibly damaging 0.88
R9647:Vmn2r-ps158 UTSW 7 42,697,171 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ATTGCACAACTGTTAGTGACATGG -3'
(R):5'- CGTCTCCATTGGCATATGGTTG -3'

Sequencing Primer
(F):5'- ACATGGAAAGTCTGGGTCTTGAACTC -3'
(R):5'- TTGAACAGTCATCTCATGGAGGC -3'
Posted On 2022-03-25