Incidental Mutation 'R9267:Polr1a'
ID |
702609 |
Institutional Source |
Beutler Lab
|
Gene Symbol |
Polr1a
|
Ensembl Gene |
ENSMUSG00000049553 |
Gene Name |
polymerase (RNA) I polypeptide A |
Synonyms |
2900087K15Rik, 3010014K16Rik, 194kDa, mRPA1, Rpo1-4, RPA194 |
MMRRC Submission |
|
Accession Numbers |
|
Essential gene? |
Essential
(E-score: 1.000)
|
Stock # |
R9267 (G1)
|
Quality Score |
225.009 |
Status
|
Validated
|
Chromosome |
6 |
Chromosomal Location |
71886037-71956419 bp(+) (GRCm39) |
Type of Mutation |
missense |
DNA Base Change (assembly) |
G to A
at 71942542 bp (GRCm39)
|
Zygosity |
Heterozygous |
Amino Acid Change |
Methionine to Isoleucine
at position 1127
(M1127I)
|
Ref Sequence |
ENSEMBL: ENSMUSP00000060858
(fasta)
|
Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000055296]
|
AlphaFold |
O35134 |
Predicted Effect |
probably benign
Transcript: ENSMUST00000055296
AA Change: M1127I
PolyPhen 2
Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
|
SMART Domains |
Protein: ENSMUSP00000060858 Gene: ENSMUSG00000049553 AA Change: M1127I
Domain | Start | End | E-Value | Type |
RPOLA_N
|
302 |
649 |
8.97e-137 |
SMART |
Pfam:RNA_pol_Rpb1_4
|
846 |
958 |
1.3e-26 |
PFAM |
Pfam:RNA_pol_Rpb1_5
|
965 |
1669 |
7e-103 |
PFAM |
low complexity region
|
1698 |
1708 |
N/A |
INTRINSIC |
|
Predicted Effect |
|
Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.6%
- 20x: 98.7%
|
Validation Efficiency |
98% (79/81) |
MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is the largest subunit of the RNA polymerase I complex. The encoded protein represents the catalytic subunit of the complex, which transcribes DNA into ribosomal RNA precursors. Defects in this gene are a cause of the Cincinnati type of acrofacial dysostosis. [provided by RefSeq, May 2016]
|
Allele List at MGI |
All alleles(18) : Gene trapped(18) |
Other mutations in this stock |
Total: 83 list
Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
Abcc4 |
G |
T |
14: 118,869,657 (GRCm39) |
|
probably benign |
Het |
Abcd2 |
T |
A |
15: 91,063,423 (GRCm39) |
T422S |
possibly damaging |
Het |
Acsl3 |
T |
G |
1: 78,674,623 (GRCm39) |
I390S |
probably damaging |
Het |
Adad1 |
T |
A |
3: 37,139,074 (GRCm39) |
|
probably benign |
Het |
Adam3 |
A |
G |
8: 25,171,605 (GRCm39) |
V742A |
probably benign |
Het |
Adgra3 |
A |
T |
5: 50,155,618 (GRCm39) |
I450K |
possibly damaging |
Het |
Adgrb2 |
T |
C |
4: 129,885,901 (GRCm39) |
S14P |
possibly damaging |
Het |
Arfgef3 |
T |
A |
10: 18,475,028 (GRCm39) |
I1553F |
probably damaging |
Het |
Btbd16 |
C |
T |
7: 130,417,516 (GRCm39) |
R344C |
probably damaging |
Het |
Ccdc27 |
A |
G |
4: 154,117,441 (GRCm39) |
L502P |
probably damaging |
Het |
Ccdc50 |
T |
A |
16: 27,208,700 (GRCm39) |
L5Q |
|
Het |
Cdh16 |
C |
T |
8: 105,341,834 (GRCm39) |
G777D |
probably damaging |
Het |
Cntn2 |
C |
A |
1: 132,449,021 (GRCm39) |
V663L |
probably benign |
Het |
Cpne4 |
T |
A |
9: 104,884,833 (GRCm39) |
V343E |
probably damaging |
Het |
Dctpp1 |
A |
T |
7: 126,856,275 (GRCm39) |
I149N |
probably benign |
Het |
Dlg5 |
T |
C |
14: 24,204,745 (GRCm39) |
N1266S |
probably damaging |
Het |
Dst |
A |
G |
1: 34,232,145 (GRCm39) |
D3246G |
probably benign |
Het |
Efcab8 |
G |
C |
2: 153,646,861 (GRCm39) |
V397L |
unknown |
Het |
Erbin |
T |
C |
13: 103,987,292 (GRCm39) |
T423A |
probably damaging |
Het |
Erich5 |
C |
T |
15: 34,471,563 (GRCm39) |
P264L |
possibly damaging |
Het |
Fam90a1a |
A |
G |
8: 22,453,091 (GRCm39) |
M149V |
probably benign |
Het |
Fbxl18 |
C |
T |
5: 142,880,870 (GRCm39) |
G62R |
possibly damaging |
Het |
Fech |
A |
G |
18: 64,591,267 (GRCm39) |
S386P |
possibly damaging |
Het |
Fhl3 |
T |
A |
4: 124,601,498 (GRCm39) |
|
probably null |
Het |
Fkbp5 |
A |
G |
17: 28,629,558 (GRCm39) |
S256P |
probably benign |
Het |
Frem2 |
A |
G |
3: 53,564,504 (GRCm39) |
M1T |
probably null |
Het |
Galnt5 |
T |
C |
2: 57,925,220 (GRCm39) |
F834L |
possibly damaging |
Het |
Gm3618 |
G |
T |
14: 17,502,588 (GRCm39) |
T99N |
unknown |
Het |
Gm9195 |
T |
A |
14: 72,700,546 (GRCm39) |
Q1127L |
possibly damaging |
Het |
Gpsm1 |
A |
G |
2: 26,236,835 (GRCm39) |
D643G |
probably damaging |
Het |
Grm4 |
A |
G |
17: 27,654,183 (GRCm39) |
V589A |
possibly damaging |
Het |
Gskip |
T |
A |
12: 105,665,052 (GRCm39) |
F30L |
probably benign |
Het |
Haus3 |
T |
A |
5: 34,311,452 (GRCm39) |
|
probably null |
Het |
Heatr1 |
T |
C |
13: 12,421,489 (GRCm39) |
F463S |
probably damaging |
Het |
Hmcn1 |
G |
A |
1: 150,473,740 (GRCm39) |
S4851F |
probably benign |
Het |
Il16 |
A |
C |
7: 83,371,757 (GRCm39) |
F16L |
probably benign |
Het |
Itga1 |
C |
A |
13: 115,185,924 (GRCm39) |
C26F |
possibly damaging |
Het |
Itga6 |
A |
G |
2: 71,668,756 (GRCm39) |
E565G |
probably benign |
Het |
Itgb4 |
A |
G |
11: 115,870,465 (GRCm39) |
H98R |
probably benign |
Het |
Kidins220 |
T |
A |
12: 25,038,558 (GRCm39) |
S37T |
probably benign |
Het |
Lrrc37 |
A |
T |
11: 103,495,406 (GRCm39) |
D2804E |
unknown |
Het |
Lrrk2 |
C |
T |
15: 91,584,629 (GRCm39) |
L349F |
probably damaging |
Het |
Malrd1 |
T |
G |
2: 16,260,077 (GRCm39) |
I2099S |
unknown |
Het |
Mapk8ip1 |
A |
G |
2: 92,216,714 (GRCm39) |
I492T |
possibly damaging |
Het |
Mest |
T |
A |
6: 30,742,141 (GRCm39) |
D68E |
|
Het |
Mthfd1l |
A |
G |
10: 3,934,154 (GRCm39) |
D162G |
probably benign |
Het |
Nalcn |
A |
T |
14: 123,518,567 (GRCm39) |
D1657E |
probably benign |
Het |
Nbeal2 |
C |
A |
9: 110,462,115 (GRCm39) |
V1475F |
probably damaging |
Het |
Nf1 |
C |
A |
11: 79,331,716 (GRCm39) |
Q684K |
possibly damaging |
Het |
Opn5 |
T |
A |
17: 42,903,793 (GRCm39) |
T181S |
probably damaging |
Het |
Or10ag54 |
T |
C |
2: 87,099,326 (GRCm39) |
L67P |
possibly damaging |
Het |
Or1j19 |
A |
T |
2: 36,676,530 (GRCm39) |
|
probably benign |
Het |
Or7a38 |
A |
G |
10: 78,752,803 (GRCm39) |
N43S |
probably damaging |
Het |
Or9s15 |
A |
T |
1: 92,524,994 (GRCm39) |
H251L |
probably damaging |
Het |
Or9s15 |
A |
T |
1: 92,524,996 (GRCm39) |
M252L |
probably benign |
Het |
Orc4 |
T |
A |
2: 48,827,534 (GRCm39) |
R20* |
probably null |
Het |
Pik3ca |
T |
C |
3: 32,492,211 (GRCm39) |
Y270H |
probably damaging |
Het |
Pkn2 |
T |
C |
3: 142,517,676 (GRCm39) |
E474G |
probably null |
Het |
Pnma8b |
A |
T |
7: 16,679,159 (GRCm39) |
N48Y |
unknown |
Het |
Pnpla2 |
A |
C |
7: 141,036,503 (GRCm39) |
|
probably benign |
Het |
Ptpre |
A |
C |
7: 135,274,549 (GRCm39) |
D507A |
probably damaging |
Het |
Rab3gap2 |
T |
C |
1: 184,983,331 (GRCm39) |
V490A |
probably damaging |
Het |
Rab44 |
A |
T |
17: 29,354,338 (GRCm39) |
|
probably benign |
Het |
Rchy1 |
G |
T |
5: 92,105,831 (GRCm39) |
T39N |
probably benign |
Het |
Ripor1 |
A |
G |
8: 106,348,063 (GRCm39) |
D1146G |
unknown |
Het |
Sh3yl1 |
A |
T |
12: 30,972,335 (GRCm39) |
I35F |
possibly damaging |
Het |
Slc22a5 |
A |
G |
11: 53,764,619 (GRCm39) |
F276L |
probably benign |
Het |
Slc8a3 |
T |
A |
12: 81,361,208 (GRCm39) |
D537V |
possibly damaging |
Het |
Smc4 |
T |
A |
3: 68,941,786 (GRCm39) |
I1263N |
probably damaging |
Het |
Snai2 |
A |
T |
16: 14,525,120 (GRCm39) |
T209S |
possibly damaging |
Het |
Stam2 |
A |
T |
2: 52,604,903 (GRCm39) |
Y211N |
probably damaging |
Het |
Stxbp1 |
A |
G |
2: 32,708,517 (GRCm39) |
S83P |
probably damaging |
Het |
Susd4 |
T |
C |
1: 182,716,389 (GRCm39) |
F325L |
probably benign |
Het |
Tmem245 |
A |
G |
4: 56,947,236 (GRCm39) |
V59A |
probably benign |
Het |
Tnk2 |
A |
G |
16: 32,494,489 (GRCm39) |
D469G |
probably damaging |
Het |
Tpx2 |
A |
G |
2: 152,732,517 (GRCm39) |
D597G |
probably damaging |
Het |
Ube2w |
A |
T |
1: 16,612,011 (GRCm39) |
S170R |
|
Het |
Vmn2r102 |
A |
T |
17: 19,896,928 (GRCm39) |
I92F |
probably damaging |
Het |
Vmn2r11 |
T |
A |
5: 109,199,929 (GRCm39) |
E508V |
possibly damaging |
Het |
Vmn2r30 |
T |
A |
7: 7,340,432 (GRCm39) |
I21F |
possibly damaging |
Het |
Yipf2 |
G |
A |
9: 21,501,489 (GRCm39) |
T187M |
possibly damaging |
Het |
Zbtb8a |
T |
A |
4: 129,248,267 (GRCm39) |
K335* |
probably null |
Het |
Zfp609 |
T |
C |
9: 65,608,328 (GRCm39) |
D1184G |
possibly damaging |
Het |
|
Other mutations in Polr1a |
Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
IGL01363:Polr1a
|
APN |
6 |
71,925,470 (GRCm39) |
missense |
probably benign |
0.32 |
IGL01834:Polr1a
|
APN |
6 |
71,925,446 (GRCm39) |
missense |
probably benign |
|
IGL01902:Polr1a
|
APN |
6 |
71,940,732 (GRCm39) |
missense |
probably damaging |
1.00 |
IGL02101:Polr1a
|
APN |
6 |
71,927,786 (GRCm39) |
missense |
probably benign |
0.00 |
IGL02325:Polr1a
|
APN |
6 |
71,897,641 (GRCm39) |
missense |
probably benign |
0.38 |
IGL02398:Polr1a
|
APN |
6 |
71,913,540 (GRCm39) |
splice site |
probably benign |
|
IGL02528:Polr1a
|
APN |
6 |
71,941,701 (GRCm39) |
missense |
probably benign |
|
IGL02555:Polr1a
|
APN |
6 |
71,897,441 (GRCm39) |
missense |
probably damaging |
0.98 |
IGL02613:Polr1a
|
APN |
6 |
71,944,304 (GRCm39) |
missense |
probably damaging |
1.00 |
IGL02693:Polr1a
|
APN |
6 |
71,940,830 (GRCm39) |
splice site |
probably benign |
|
IGL02892:Polr1a
|
APN |
6 |
71,908,680 (GRCm39) |
missense |
possibly damaging |
0.70 |
IGL03059:Polr1a
|
APN |
6 |
71,913,496 (GRCm39) |
missense |
probably benign |
|
IGL03174:Polr1a
|
APN |
6 |
71,954,331 (GRCm39) |
missense |
possibly damaging |
0.82 |
D4043:Polr1a
|
UTSW |
6 |
71,918,401 (GRCm39) |
missense |
possibly damaging |
0.92 |
R0092:Polr1a
|
UTSW |
6 |
71,944,439 (GRCm39) |
splice site |
probably benign |
|
R0217:Polr1a
|
UTSW |
6 |
71,940,687 (GRCm39) |
missense |
probably benign |
0.19 |
R0267:Polr1a
|
UTSW |
6 |
71,951,123 (GRCm39) |
missense |
probably damaging |
0.99 |
R0329:Polr1a
|
UTSW |
6 |
71,943,400 (GRCm39) |
missense |
possibly damaging |
0.96 |
R0330:Polr1a
|
UTSW |
6 |
71,943,400 (GRCm39) |
missense |
possibly damaging |
0.96 |
R0352:Polr1a
|
UTSW |
6 |
71,897,747 (GRCm39) |
splice site |
probably benign |
|
R0411:Polr1a
|
UTSW |
6 |
71,955,405 (GRCm39) |
missense |
possibly damaging |
0.95 |
R0446:Polr1a
|
UTSW |
6 |
71,927,648 (GRCm39) |
critical splice donor site |
probably null |
|
R0846:Polr1a
|
UTSW |
6 |
71,901,627 (GRCm39) |
missense |
probably damaging |
1.00 |
R1035:Polr1a
|
UTSW |
6 |
71,944,900 (GRCm39) |
missense |
probably benign |
|
R1294:Polr1a
|
UTSW |
6 |
71,889,886 (GRCm39) |
missense |
probably damaging |
0.99 |
R1460:Polr1a
|
UTSW |
6 |
71,918,368 (GRCm39) |
missense |
probably damaging |
0.99 |
R1657:Polr1a
|
UTSW |
6 |
71,918,519 (GRCm39) |
missense |
probably damaging |
1.00 |
R1846:Polr1a
|
UTSW |
6 |
71,953,172 (GRCm39) |
missense |
probably damaging |
0.98 |
R1862:Polr1a
|
UTSW |
6 |
71,886,187 (GRCm39) |
missense |
probably damaging |
0.96 |
R1865:Polr1a
|
UTSW |
6 |
71,943,508 (GRCm39) |
missense |
probably damaging |
1.00 |
R1903:Polr1a
|
UTSW |
6 |
71,944,898 (GRCm39) |
missense |
probably benign |
0.02 |
R1937:Polr1a
|
UTSW |
6 |
71,913,536 (GRCm39) |
critical splice donor site |
probably null |
|
R2063:Polr1a
|
UTSW |
6 |
71,913,269 (GRCm39) |
splice site |
probably null |
|
R2071:Polr1a
|
UTSW |
6 |
71,953,058 (GRCm39) |
missense |
possibly damaging |
0.64 |
R2084:Polr1a
|
UTSW |
6 |
71,927,793 (GRCm39) |
missense |
possibly damaging |
0.69 |
R2377:Polr1a
|
UTSW |
6 |
71,949,810 (GRCm39) |
critical splice donor site |
probably null |
|
R2410:Polr1a
|
UTSW |
6 |
71,951,866 (GRCm39) |
missense |
probably benign |
|
R3001:Polr1a
|
UTSW |
6 |
71,890,000 (GRCm39) |
missense |
probably benign |
0.01 |
R3001:Polr1a
|
UTSW |
6 |
71,942,628 (GRCm39) |
missense |
probably benign |
0.02 |
R3002:Polr1a
|
UTSW |
6 |
71,942,628 (GRCm39) |
missense |
probably benign |
0.02 |
R3002:Polr1a
|
UTSW |
6 |
71,890,000 (GRCm39) |
missense |
probably benign |
0.01 |
R3924:Polr1a
|
UTSW |
6 |
71,906,434 (GRCm39) |
missense |
probably benign |
0.00 |
R4105:Polr1a
|
UTSW |
6 |
71,953,175 (GRCm39) |
missense |
probably damaging |
0.98 |
R4125:Polr1a
|
UTSW |
6 |
71,942,690 (GRCm39) |
missense |
probably benign |
0.00 |
R4271:Polr1a
|
UTSW |
6 |
71,930,006 (GRCm39) |
missense |
probably benign |
0.02 |
R4440:Polr1a
|
UTSW |
6 |
71,927,832 (GRCm39) |
missense |
probably damaging |
0.98 |
R4667:Polr1a
|
UTSW |
6 |
71,894,805 (GRCm39) |
missense |
probably benign |
0.30 |
R4769:Polr1a
|
UTSW |
6 |
71,927,852 (GRCm39) |
missense |
probably benign |
0.01 |
R4801:Polr1a
|
UTSW |
6 |
71,953,054 (GRCm39) |
missense |
probably benign |
0.00 |
R4802:Polr1a
|
UTSW |
6 |
71,953,054 (GRCm39) |
missense |
probably benign |
0.00 |
R4828:Polr1a
|
UTSW |
6 |
71,943,385 (GRCm39) |
missense |
possibly damaging |
0.93 |
R4911:Polr1a
|
UTSW |
6 |
71,886,213 (GRCm39) |
missense |
possibly damaging |
0.67 |
R5071:Polr1a
|
UTSW |
6 |
71,908,693 (GRCm39) |
missense |
possibly damaging |
0.71 |
R5165:Polr1a
|
UTSW |
6 |
71,944,909 (GRCm39) |
missense |
probably damaging |
1.00 |
R5223:Polr1a
|
UTSW |
6 |
71,944,891 (GRCm39) |
missense |
possibly damaging |
0.73 |
R5239:Polr1a
|
UTSW |
6 |
71,890,021 (GRCm39) |
missense |
probably damaging |
1.00 |
R5546:Polr1a
|
UTSW |
6 |
71,906,350 (GRCm39) |
missense |
possibly damaging |
0.64 |
R5599:Polr1a
|
UTSW |
6 |
71,944,346 (GRCm39) |
missense |
possibly damaging |
0.95 |
R5696:Polr1a
|
UTSW |
6 |
71,906,410 (GRCm39) |
missense |
probably benign |
0.05 |
R5850:Polr1a
|
UTSW |
6 |
71,903,667 (GRCm39) |
missense |
probably benign |
0.00 |
R6274:Polr1a
|
UTSW |
6 |
71,931,874 (GRCm39) |
splice site |
probably null |
|
R6526:Polr1a
|
UTSW |
6 |
71,906,427 (GRCm39) |
missense |
possibly damaging |
0.89 |
R6578:Polr1a
|
UTSW |
6 |
71,953,025 (GRCm39) |
missense |
possibly damaging |
0.93 |
R6660:Polr1a
|
UTSW |
6 |
71,944,358 (GRCm39) |
missense |
probably damaging |
0.98 |
R6892:Polr1a
|
UTSW |
6 |
71,941,696 (GRCm39) |
missense |
possibly damaging |
0.72 |
R7274:Polr1a
|
UTSW |
6 |
71,897,500 (GRCm39) |
nonsense |
probably null |
|
R7291:Polr1a
|
UTSW |
6 |
71,918,440 (GRCm39) |
missense |
probably benign |
0.02 |
R7311:Polr1a
|
UTSW |
6 |
71,927,863 (GRCm39) |
missense |
possibly damaging |
0.53 |
R7431:Polr1a
|
UTSW |
6 |
71,903,643 (GRCm39) |
missense |
probably benign |
0.14 |
R7479:Polr1a
|
UTSW |
6 |
71,913,281 (GRCm39) |
missense |
probably damaging |
1.00 |
R7607:Polr1a
|
UTSW |
6 |
71,890,005 (GRCm39) |
missense |
probably benign |
|
R7739:Polr1a
|
UTSW |
6 |
71,931,819 (GRCm39) |
missense |
possibly damaging |
0.94 |
R7746:Polr1a
|
UTSW |
6 |
71,918,496 (GRCm39) |
missense |
probably damaging |
1.00 |
R7764:Polr1a
|
UTSW |
6 |
71,930,054 (GRCm39) |
missense |
probably damaging |
1.00 |
R7835:Polr1a
|
UTSW |
6 |
71,892,126 (GRCm39) |
missense |
probably benign |
0.02 |
R8029:Polr1a
|
UTSW |
6 |
71,889,940 (GRCm39) |
nonsense |
probably null |
|
R8057:Polr1a
|
UTSW |
6 |
71,908,644 (GRCm39) |
missense |
possibly damaging |
0.95 |
R8144:Polr1a
|
UTSW |
6 |
71,927,600 (GRCm39) |
missense |
probably benign |
|
R8170:Polr1a
|
UTSW |
6 |
71,897,733 (GRCm39) |
missense |
probably benign |
|
R8320:Polr1a
|
UTSW |
6 |
71,918,368 (GRCm39) |
missense |
probably damaging |
0.99 |
R8328:Polr1a
|
UTSW |
6 |
71,897,718 (GRCm39) |
missense |
probably benign |
|
R8331:Polr1a
|
UTSW |
6 |
71,953,163 (GRCm39) |
missense |
probably damaging |
1.00 |
R8362:Polr1a
|
UTSW |
6 |
71,941,651 (GRCm39) |
missense |
probably benign |
0.00 |
R8511:Polr1a
|
UTSW |
6 |
71,897,504 (GRCm39) |
missense |
probably benign |
0.01 |
R8709:Polr1a
|
UTSW |
6 |
71,951,832 (GRCm39) |
missense |
probably benign |
|
R8745:Polr1a
|
UTSW |
6 |
71,931,755 (GRCm39) |
missense |
probably damaging |
1.00 |
R8784:Polr1a
|
UTSW |
6 |
71,927,612 (GRCm39) |
missense |
probably benign |
|
R9055:Polr1a
|
UTSW |
6 |
71,892,053 (GRCm39) |
missense |
possibly damaging |
0.46 |
R9088:Polr1a
|
UTSW |
6 |
71,908,767 (GRCm39) |
missense |
probably benign |
0.26 |
R9211:Polr1a
|
UTSW |
6 |
71,943,521 (GRCm39) |
missense |
probably damaging |
1.00 |
R9228:Polr1a
|
UTSW |
6 |
71,931,755 (GRCm39) |
missense |
probably damaging |
1.00 |
R9240:Polr1a
|
UTSW |
6 |
71,940,661 (GRCm39) |
nonsense |
probably null |
|
R9302:Polr1a
|
UTSW |
6 |
71,901,683 (GRCm39) |
critical splice donor site |
probably null |
|
R9744:Polr1a
|
UTSW |
6 |
71,906,372 (GRCm39) |
missense |
probably benign |
0.05 |
|
Predicted Primers |
PCR Primer
(F):5'- TGCTATGCTTTGGACAGGCC -3'
(R):5'- CTGCCCATTCCTGACTGAAG -3'
Sequencing Primer
(F):5'- CAGGCCAAGGTGAATTGCC -3'
(R):5'- GCAAAGTAGATGTCTGGG -3'
|
Posted On |
2022-03-25 |