Mutagenetix > Incidental Mutation

Incidental Mutation 'R9269:Zfp442'

702728

Institutional Source

Beutler Lab

Gene Symbol

Zfp442

Ensembl Gene

ENSMUSG00000068130

Gene Name

zinc finger protein 442

Synonyms

OTTMUSG00000015730

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R9269 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

150407141-150451486 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 150409367 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 205 (H205L)

Ref Sequence

ENSEMBL: ENSMUSP00000105542 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109916] [ENSMUST00000185796]

AlphaFold

A2AQA0

Predicted Effect

probably benign
Transcript: ENSMUST00000109916
AA Change: H205L

PolyPhen 2 Score 0.187 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130
AA Change: H205L

Domain	Start	End	E-Value	Type
KRAB	4	66	3.27e-19	SMART
ZnF_C2H2	159	181	8.34e-3	SMART
ZnF_C2H2	211	233	9.58e-3	SMART
ZnF_C2H2	239	261	2.43e-4	SMART
ZnF_C2H2	267	289	1.38e-3	SMART
ZnF_C2H2	295	317	4.17e-3	SMART
ZnF_C2H2	323	345	3.16e-3	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	9.58e-3	SMART
ZnF_C2H2	407	429	2.09e-3	SMART
ZnF_C2H2	435	457	2.2e-2	SMART
ZnF_C2H2	463	485	1.6e-4	SMART
ZnF_C2H2	491	513	1.82e-3	SMART
ZnF_C2H2	519	541	4.47e-3	SMART
ZnF_C2H2	547	569	3.63e-3	SMART
ZnF_C2H2	575	597	4.79e-3	SMART
ZnF_C2H2	603	625	8.47e-4	SMART
ZnF_C2H2	631	654	3.11e-2	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000185796
AA Change: H204L

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000140098
Gene: ENSMUSG00000068130
AA Change: H204L

Domain	Start	End	E-Value	Type
KRAB	3	65	1.4e-21	SMART
ZnF_C2H2	158	180	3.4e-5	SMART
ZnF_C2H2	210	232	3.9e-5	SMART
ZnF_C2H2	238	260	1e-6	SMART
ZnF_C2H2	266	288	5.6e-6	SMART
ZnF_C2H2	294	316	1.8e-5	SMART
ZnF_C2H2	322	344	1.3e-5	SMART
ZnF_C2H2	350	372	6.7e-6	SMART
ZnF_C2H2	378	400	9.6e-5	SMART
ZnF_C2H2	406	428	6.9e-7	SMART
ZnF_C2H2	434	456	7.7e-6	SMART
ZnF_C2H2	462	484	1.9e-5	SMART
ZnF_C2H2	490	512	1.5e-5	SMART
ZnF_C2H2	518	540	2e-5	SMART
ZnF_C2H2	546	568	3.5e-6	SMART
ZnF_C2H2	574	597	1.3e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	T	C	6: 121,660,906 (GRCm38)	S845P	probably benign	Het
Actn1	T	A	12: 80,172,971 (GRCm38)	N709Y	probably benign	Het
Alox5ap	A	G	5: 149,279,196 (GRCm38)	Y64C	probably damaging	Het
Anapc4	T	A	5: 52,861,278 (GRCm38)	S521T	possibly damaging	Het
Ap3b1	C	A	13: 94,404,062 (GRCm38)	P164Q	probably damaging	Het
Arhgef26	A	G	3: 62,340,499 (GRCm38)	N335D	probably damaging	Het
Arrdc2	G	A	8: 70,836,329 (GRCm38)	A354V	probably benign	Het
Atg2b	A	G	12: 105,652,100 (GRCm38)	S898P	probably damaging	Het
Atxn1	T	A	13: 45,557,204 (GRCm38)	T751S	probably benign	Het
Begain	T	A	12: 109,033,193 (GRCm38)	R551W	possibly damaging	Het
Borcs8	C	A	8: 70,141,871 (GRCm38)	D15E	probably benign	Het
Btbd16	C	T	7: 130,815,786 (GRCm38)	R344C	probably damaging	Het
Card11	A	T	5: 140,906,761 (GRCm38)	M183K	probably damaging	Het
Cldn3	T	C	5: 134,986,725 (GRCm38)	L94P	probably damaging	Het
Clvs2	A	T	10: 33,543,426 (GRCm38)	Y211N	probably damaging	Het
Col15a1	G	C	4: 47,288,200 (GRCm38)		probably benign	Het
Cop1	C	T	1: 159,288,983 (GRCm38)	P409L	probably benign	Het
Cplane1	A	C	15: 8,219,016 (GRCm38)	Q1683P	probably damaging	Het
Crhbp	C	A	13: 95,436,516 (GRCm38)	A241S	probably benign	Het
Cyfip1	C	A	7: 55,907,431 (GRCm38)	S794*	probably null	Het
Dlg5	T	A	14: 24,192,813 (GRCm38)	H172L	probably damaging	Het
Doc2a	T	A	7: 126,850,987 (GRCm38)	I199N	probably benign	Het
Dock3	T	C	9: 106,941,323 (GRCm38)	T1191A	probably benign	Het
Efcab8	G	C	2: 153,804,941 (GRCm38)	V397L	unknown	Het
Eif4a3l2	C	T	6: 116,552,356 (GRCm38)	T398I	probably damaging	Het
Fcgr1	C	T	3: 96,285,838 (GRCm38)	R281H	probably benign	Het
Fndc10	T	C	4: 155,694,748 (GRCm38)	V83A	possibly damaging	Het
Galnt2	T	A	8: 124,338,463 (GRCm38)	I444K	probably benign	Het
Gcnt3	T	C	9: 70,034,008 (GRCm38)	Y426C	probably damaging	Het
Gtpbp1	C	T	15: 79,717,654 (GRCm38)	R533C	probably damaging	Het
Hid1	T	C	11: 115,361,676 (GRCm38)	E60G	probably damaging	Het
Ighv1-81	A	T	12: 115,920,541 (GRCm38)	L30Q	possibly damaging	Het
Ighv5-8	TATATATATATATATATATATA	TATATATATATATATATATATATA	12: 113,654,945 (GRCm38)		probably null	Het
Ilrun	A	C	17: 27,786,075 (GRCm38)	Y169*	probably null	Het
Klf10	A	G	15: 38,297,758 (GRCm38)	L44P	probably damaging	Het
Lamc3	T	C	2: 31,923,005 (GRCm38)	V1001A	probably benign	Het
Lamc3	C	A	2: 31,928,896 (GRCm38)	S1211*	probably null	Het
Lmx1a	T	A	1: 167,830,625 (GRCm38)	H192Q	probably benign	Het
Nkx2-4	T	C	2: 147,084,264 (GRCm38)	H226R	possibly damaging	Het
Or2d2	T	C	7: 107,128,626 (GRCm38)	I256V	probably benign	Het
Or4a68	T	A	2: 89,439,932 (GRCm38)	M116L	probably damaging	Het
Or4k37	T	C	2: 111,328,952 (GRCm38)	F178L	probably damaging	Het
Or4p18	G	A	2: 88,402,242 (GRCm38)	R231C	probably benign	Het
Or5b101	T	C	19: 13,027,630 (GRCm38)	H233R	probably benign	Het
Or5b114-ps1	A	T	19: 13,375,640 (GRCm38)	N226I	unknown	Het
Or5p52	T	A	7: 107,903,320 (GRCm38)	I201N	possibly damaging	Het
Or7e175	T	C	9: 20,137,461 (GRCm38)	M115T	probably damaging	Het
Or8k1	T	C	2: 86,216,903 (GRCm38)	H269R	probably damaging	Het
Orai3	G	T	7: 127,774,022 (GRCm38)	A232S	probably benign	Het
Pak1ip1	G	A	13: 41,009,251 (GRCm38)	G177R	probably benign	Het
Pcp4l1	T	C	1: 171,174,406 (GRCm38)	R62G	possibly damaging	Het
Phox2b	T	A	5: 67,098,721 (GRCm38)	Q74L	probably benign	Het
Pi4kb	A	G	3: 94,984,486 (GRCm38)	Y159C	probably damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195 (GRCm38)		probably benign	Het
Plxna1	T	C	6: 89,329,559 (GRCm38)	R1430G	probably null	Het
Plxna4	T	C	6: 32,178,380 (GRCm38)	H1543R	probably benign	Het
Prcc	A	T	3: 87,869,731 (GRCm38)	F312Y	probably damaging	Het
Ranbp6	A	G	19: 29,809,988 (GRCm38)	L988P	probably damaging	Het
Rbm27	A	G	18: 42,327,507 (GRCm38)	T840A	probably benign	Het
Rchy1	G	T	5: 91,957,972 (GRCm38)	T39N	probably benign	Het
Rmi1	C	T	13: 58,409,026 (GRCm38)	T363I	probably benign	Het
Rnpc3	A	C	3: 113,611,246 (GRCm38)	I420S	probably damaging	Het
Rsph4a	T	A	10: 33,909,398 (GRCm38)	V435E	probably benign	Het
Rwdd1	T	C	10: 34,012,099 (GRCm38)	T38A	probably damaging	Het
S100a13	G	T	3: 90,515,863 (GRCm38)	D54Y	unknown	Het
Sel1l3	T	C	5: 53,154,286 (GRCm38)	Y619C	probably damaging	Het
Sf3b3	T	C	8: 110,812,026 (GRCm38)	T1121A	probably damaging	Het
Sgk3	T	C	1: 9,872,309 (GRCm38)	V102A	probably benign	Het
Sh2b1	T	C	7: 126,469,182 (GRCm38)	T486A	probably damaging	Het
Slc22a5	C	T	11: 53,876,155 (GRCm38)	R169H	probably damaging	Het
Slc4a5	T	C	6: 83,289,241 (GRCm38)	V889A	possibly damaging	Het
Spast	C	A	17: 74,339,074 (GRCm38)	S13*	probably null	Het
Srek1	T	C	13: 103,753,146 (GRCm38)		probably null	Het
Sugp1	G	T	8: 70,056,570 (GRCm38)	E164D	probably benign	Het
Synpo2	T	C	3: 123,117,324 (GRCm38)	D224G	probably benign	Het
Tanc1	A	G	2: 59,800,088 (GRCm38)	D804G	probably damaging	Het
Tep1	C	T	14: 50,844,309 (GRCm38)	C1228Y	probably damaging	Het
Tes	T	A	6: 17,100,342 (GRCm38)	C325S	probably benign	Het
Themis	T	C	10: 28,863,394 (GRCm38)	V620A	probably benign	Het
Trim17	T	C	11: 58,971,431 (GRCm38)	S430P	probably damaging	Het
Tubgcp5	T	C	7: 55,795,945 (GRCm38)	I65T	possibly damaging	Het
Unc13b	T	C	4: 43,171,955 (GRCm38)	S928P	unknown	Het
Vat1l	C	T	8: 114,289,432 (GRCm38)	A354V	probably damaging	Het
Vmn1r170	T	A	7: 23,606,838 (GRCm38)	S222T	probably benign	Het
Vmn2r112	A	T	17: 22,601,232 (GRCm38)	M29L	probably benign	Het
Vmn2r72	T	C	7: 85,751,203 (GRCm38)	I213V	probably benign	Het
Wdr89	T	A	12: 75,632,790 (GRCm38)	Q230L	possibly damaging	Het
Zfp386	A	G	12: 116,059,663 (GRCm38)	T334A	probably benign	Het
Zfyve26	A	T	12: 79,276,302 (GRCm38)	I890N	possibly damaging	Het
Zic1	T	C	9: 91,364,320 (GRCm38)	E233G	probably damaging	Het

Other mutations in Zfp442

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Zfp442	APN	2	150,409,347 (GRCm38)	nonsense	probably null
IGL02566:Zfp442	APN	2	150,409,791 (GRCm38)	critical splice acceptor site	probably null
IGL03217:Zfp442	APN	2	150,409,794 (GRCm38)	splice site	probably benign
LCD18:Zfp442	UTSW	2	150,419,848 (GRCm38)	intron	probably benign
PIT4812001:Zfp442	UTSW	2	150,409,741 (GRCm38)	nonsense	probably null
R0219:Zfp442	UTSW	2	150,411,240 (GRCm38)	missense	probably damaging	0.99
R0521:Zfp442	UTSW	2	150,411,249 (GRCm38)	missense	possibly damaging	0.92
R1633:Zfp442	UTSW	2	150,408,340 (GRCm38)	nonsense	probably null
R1702:Zfp442	UTSW	2	150,409,180 (GRCm38)	nonsense	probably null
R1829:Zfp442	UTSW	2	150,409,063 (GRCm38)	missense	probably damaging	0.99
R1868:Zfp442	UTSW	2	150,408,180 (GRCm38)	missense	probably damaging	1.00
R1898:Zfp442	UTSW	2	150,408,662 (GRCm38)	missense	probably damaging	1.00
R2030:Zfp442	UTSW	2	150,408,122 (GRCm38)	missense	possibly damaging	0.58
R4676:Zfp442	UTSW	2	150,409,606 (GRCm38)	missense	probably damaging	1.00
R4717:Zfp442	UTSW	2	150,408,229 (GRCm38)	missense	probably damaging	1.00
R4894:Zfp442	UTSW	2	150,411,210 (GRCm38)	critical splice donor site	probably null
R4932:Zfp442	UTSW	2	150,409,715 (GRCm38)	missense	possibly damaging	0.53
R4963:Zfp442	UTSW	2	150,408,495 (GRCm38)	missense	probably damaging	1.00
R5130:Zfp442	UTSW	2	150,409,610 (GRCm38)	missense	possibly damaging	0.91
R5476:Zfp442	UTSW	2	150,408,159 (GRCm38)	missense	probably damaging	1.00
R5986:Zfp442	UTSW	2	150,408,024 (GRCm38)	nonsense	probably null
R6042:Zfp442	UTSW	2	150,408,096 (GRCm38)	missense	probably damaging	0.97
R6383:Zfp442	UTSW	2	150,451,401 (GRCm38)	critical splice donor site	probably null
R6452:Zfp442	UTSW	2	150,408,108 (GRCm38)	missense	probably damaging	1.00
R6787:Zfp442	UTSW	2	150,409,579 (GRCm38)	missense	possibly damaging	0.72
R6931:Zfp442	UTSW	2	150,410,940 (GRCm38)	critical splice donor site	probably null
R7061:Zfp442	UTSW	2	150,408,017 (GRCm38)	missense	probably benign	0.33
R7184:Zfp442	UTSW	2	150,408,136 (GRCm38)	missense	possibly damaging	0.71
R7214:Zfp442	UTSW	2	150,409,281 (GRCm38)	missense	probably benign	0.04
R7225:Zfp442	UTSW	2	150,409,005 (GRCm38)	missense	probably benign	0.00
R7513:Zfp442	UTSW	2	150,408,756 (GRCm38)	missense	unknown
R7591:Zfp442	UTSW	2	150,408,172 (GRCm38)	nonsense	probably null
R7679:Zfp442	UTSW	2	150,410,997 (GRCm38)	nonsense	probably null
R7768:Zfp442	UTSW	2	150,408,321 (GRCm38)	missense	possibly damaging	0.53
R7801:Zfp442	UTSW	2	150,409,719 (GRCm38)	missense	probably benign	0.28
R7814:Zfp442	UTSW	2	150,409,482 (GRCm38)	missense	possibly damaging	0.92
R7848:Zfp442	UTSW	2	150,411,226 (GRCm38)	missense	possibly damaging	0.71
R8158:Zfp442	UTSW	2	150,409,176 (GRCm38)	missense	possibly damaging	0.83
R8192:Zfp442	UTSW	2	150,408,709 (GRCm38)	missense	unknown
R8528:Zfp442	UTSW	2	150,409,042 (GRCm38)	missense	probably damaging	1.00
R9110:Zfp442	UTSW	2	150,408,173 (GRCm38)	missense	probably benign	0.30
R9371:Zfp442	UTSW	2	150,408,756 (GRCm38)	missense	unknown
R9401:Zfp442	UTSW	2	150,409,695 (GRCm38)	missense	possibly damaging	0.53
R9459:Zfp442	UTSW	2	150,408,748 (GRCm38)	missense	unknown
R9711:Zfp442	UTSW	2	150,408,287 (GRCm38)	missense	possibly damaging	0.93
Z1177:Zfp442	UTSW	2	150,408,479 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- CCACATTGCTTACATTCATAGGG -3'
(R):5'- ACCCTCTGAATTTACTCAATGTGGT -3'

Sequencing Primer
(F):5'- ATTCATAGGGTTTCTCTCCAGTATG -3'
(R):5'- AAGTCTTTGCGTATCAGAGCC -3'

Posted On

2022-03-25