Mutagenetix > Incidental Mutation

Incidental Mutation 'R9269:Unc13b'

702737

Institutional Source

Beutler Lab

Gene Symbol

Unc13b

Ensembl Gene

ENSMUSG00000028456

Gene Name

unc-13 homolog B (C. elegans)

Synonyms

Unc13h2, Munc13-2

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.317) question?

Stock #

R9269 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

43058953-43264871 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 43171955 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 928 (S928P)

Ref Sequence

ENSEMBL: ENSMUSP00000147100 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000079978] [ENSMUST00000107952] [ENSMUST00000107953] [ENSMUST00000163653] [ENSMUST00000207569]

AlphaFold

Q9Z1N9

Predicted Effect

probably benign
Transcript: ENSMUST00000079978

SMART Domains

Protein: ENSMUSP00000078894
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	179	193	N/A	INTRINSIC
low complexity region	292	303	N/A	INTRINSIC
low complexity region	322	333	N/A	INTRINSIC
C1	478	527	4.21e-18	SMART
C2	601	708	2.07e-22	SMART
DUF1041	917	1021	2.02e-53	SMART
Pfam:Membr_traf_MHD	1262	1404	4.8e-60	PFAM
C2	1438	1544	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107952

SMART Domains

Protein: ENSMUSP00000103586
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	191	205	N/A	INTRINSIC
low complexity region	304	315	N/A	INTRINSIC
low complexity region	334	345	N/A	INTRINSIC
C1	490	539	4.21e-18	SMART
C2	613	720	2.07e-22	SMART
DUF1041	929	1033	2.02e-53	SMART
Pfam:Membr_traf_MHD	1274	1416	4.8e-60	PFAM
C2	1450	1556	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107953

SMART Domains

Protein: ENSMUSP00000103587
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	179	193	N/A	INTRINSIC
low complexity region	292	303	N/A	INTRINSIC
low complexity region	322	333	N/A	INTRINSIC
C1	478	527	4.21e-18	SMART
C2	601	708	2.07e-22	SMART
DUF1041	917	1021	2.02e-53	SMART
Pfam:Membr_traf_MHD	1263	1403	2.3e-56	PFAM
C2	1457	1563	7.56e-16	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000163653

SMART Domains

Protein: ENSMUSP00000128608
Gene: ENSMUSG00000028456

Domain	Start	End	E-Value	Type
C2	3	94	1.2e-9	SMART
low complexity region	191	205	N/A	INTRINSIC
low complexity region	304	315	N/A	INTRINSIC
low complexity region	334	345	N/A	INTRINSIC
C1	490	539	4.21e-18	SMART
C2	613	720	2.07e-22	SMART
DUF1041	929	1032	4.64e-53	SMART
Pfam:Membr_traf_MHD	1273	1415	4.8e-60	PFAM
C2	1449	1555	7.56e-16	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000207569
AA Change: S928P

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous mutant mice are grossly phenotypically normal. Mice older than 12 months will exhibit sporadic seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	A	C	15: 8,219,016 (GRCm38)	Q1683P	probably damaging	Het
A2m	T	C	6: 121,660,906 (GRCm38)	S845P	probably benign	Het
Actn1	T	A	12: 80,172,971 (GRCm38)	N709Y	probably benign	Het
Alox5ap	A	G	5: 149,279,196 (GRCm38)	Y64C	probably damaging	Het
Anapc4	T	A	5: 52,861,278 (GRCm38)	S521T	possibly damaging	Het
Ap3b1	C	A	13: 94,404,062 (GRCm38)	P164Q	probably damaging	Het
Arhgef26	A	G	3: 62,340,499 (GRCm38)	N335D	probably damaging	Het
Arrdc2	G	A	8: 70,836,329 (GRCm38)	A354V	probably benign	Het
Atg2b	A	G	12: 105,652,100 (GRCm38)	S898P	probably damaging	Het
Atxn1	T	A	13: 45,557,204 (GRCm38)	T751S	probably benign	Het
Begain	T	A	12: 109,033,193 (GRCm38)	R551W	possibly damaging	Het
Borcs8	C	A	8: 70,141,871 (GRCm38)	D15E	probably benign	Het
Btbd16	C	T	7: 130,815,786 (GRCm38)	R344C	probably damaging	Het
Card11	A	T	5: 140,906,761 (GRCm38)	M183K	probably damaging	Het
Cldn3	T	C	5: 134,986,725 (GRCm38)	L94P	probably damaging	Het
Clvs2	A	T	10: 33,543,426 (GRCm38)	Y211N	probably damaging	Het
Col15a1	G	C	4: 47,288,200 (GRCm38)		probably benign	Het
Cop1	C	T	1: 159,288,983 (GRCm38)	P409L	probably benign	Het
Crhbp	C	A	13: 95,436,516 (GRCm38)	A241S	probably benign	Het
Cyfip1	C	A	7: 55,907,431 (GRCm38)	S794*	probably null	Het
D17Wsu92e	A	C	17: 27,786,075 (GRCm38)	Y169*	probably null	Het
Dlg5	T	A	14: 24,192,813 (GRCm38)	H172L	probably damaging	Het
Doc2a	T	A	7: 126,850,987 (GRCm38)	I199N	probably benign	Het
Dock3	T	C	9: 106,941,323 (GRCm38)	T1191A	probably benign	Het
Efcab8	G	C	2: 153,804,941 (GRCm38)	V397L	unknown	Het
Fcgr1	C	T	3: 96,285,838 (GRCm38)	R281H	probably benign	Het
Fndc10	T	C	4: 155,694,748 (GRCm38)	V83A	possibly damaging	Het
Galnt2	T	A	8: 124,338,463 (GRCm38)	I444K	probably benign	Het
Gcnt3	T	C	9: 70,034,008 (GRCm38)	Y426C	probably damaging	Het
Gm5580	C	T	6: 116,552,356 (GRCm38)	T398I	probably damaging	Het
Gtpbp1	C	T	15: 79,717,654 (GRCm38)	R533C	probably damaging	Het
Hid1	T	C	11: 115,361,676 (GRCm38)	E60G	probably damaging	Het
Ighv1-81	A	T	12: 115,920,541 (GRCm38)	L30Q	possibly damaging	Het
Ighv5-8	TATATATATATATATATATATA	TATATATATATATATATATATATA	12: 113,654,945 (GRCm38)		probably null	Het
Klf10	A	G	15: 38,297,758 (GRCm38)	L44P	probably damaging	Het
Lamc3	C	A	2: 31,928,896 (GRCm38)	S1211*	probably null	Het
Lamc3	T	C	2: 31,923,005 (GRCm38)	V1001A	probably benign	Het
Lmx1a	T	A	1: 167,830,625 (GRCm38)	H192Q	probably benign	Het
Nkx2-4	T	C	2: 147,084,264 (GRCm38)	H226R	possibly damaging	Het
Olfr1046	T	C	2: 86,216,903 (GRCm38)	H269R	probably damaging	Het
Olfr1179	G	A	2: 88,402,242 (GRCm38)	R231C	probably benign	Het
Olfr1240	T	A	2: 89,439,932 (GRCm38)	M116L	probably damaging	Het
Olfr1281	T	C	2: 111,328,952 (GRCm38)	F178L	probably damaging	Het
Olfr1453	T	C	19: 13,027,630 (GRCm38)	H233R	probably benign	Het
Olfr1468-ps1	A	T	19: 13,375,640 (GRCm38)	N226I	unknown	Het
Olfr472	T	A	7: 107,903,320 (GRCm38)	I201N	possibly damaging	Het
Olfr715	T	C	7: 107,128,626 (GRCm38)	I256V	probably benign	Het
Olfr869	T	C	9: 20,137,461 (GRCm38)	M115T	probably damaging	Het
Orai3	G	T	7: 127,774,022 (GRCm38)	A232S	probably benign	Het
Pak1ip1	G	A	13: 41,009,251 (GRCm38)	G177R	probably benign	Het
Pcp4l1	T	C	1: 171,174,406 (GRCm38)	R62G	possibly damaging	Het
Phox2b	T	A	5: 67,098,721 (GRCm38)	Q74L	probably benign	Het
Pi4kb	A	G	3: 94,984,486 (GRCm38)	Y159C	probably damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195 (GRCm38)		probably benign	Het
Plxna1	T	C	6: 89,329,559 (GRCm38)	R1430G	probably null	Het
Plxna4	T	C	6: 32,178,380 (GRCm38)	H1543R	probably benign	Het
Prcc	A	T	3: 87,869,731 (GRCm38)	F312Y	probably damaging	Het
Ranbp6	A	G	19: 29,809,988 (GRCm38)	L988P	probably damaging	Het
Rbm27	A	G	18: 42,327,507 (GRCm38)	T840A	probably benign	Het
Rchy1	G	T	5: 91,957,972 (GRCm38)	T39N	probably benign	Het
Rmi1	C	T	13: 58,409,026 (GRCm38)	T363I	probably benign	Het
Rnpc3	A	C	3: 113,611,246 (GRCm38)	I420S	probably damaging	Het
Rsph4a	T	A	10: 33,909,398 (GRCm38)	V435E	probably benign	Het
Rwdd1	T	C	10: 34,012,099 (GRCm38)	T38A	probably damaging	Het
S100a13	G	T	3: 90,515,863 (GRCm38)	D54Y	unknown	Het
Sel1l3	T	C	5: 53,154,286 (GRCm38)	Y619C	probably damaging	Het
Sf3b3	T	C	8: 110,812,026 (GRCm38)	T1121A	probably damaging	Het
Sgk3	T	C	1: 9,872,309 (GRCm38)	V102A	probably benign	Het
Sh2b1	T	C	7: 126,469,182 (GRCm38)	T486A	probably damaging	Het
Slc22a5	C	T	11: 53,876,155 (GRCm38)	R169H	probably damaging	Het
Slc4a5	T	C	6: 83,289,241 (GRCm38)	V889A	possibly damaging	Het
Spast	C	A	17: 74,339,074 (GRCm38)	S13*	probably null	Het
Srek1	T	C	13: 103,753,146 (GRCm38)		probably null	Het
Sugp1	G	T	8: 70,056,570 (GRCm38)	E164D	probably benign	Het
Synpo2	T	C	3: 123,117,324 (GRCm38)	D224G	probably benign	Het
Tanc1	A	G	2: 59,800,088 (GRCm38)	D804G	probably damaging	Het
Tep1	C	T	14: 50,844,309 (GRCm38)	C1228Y	probably damaging	Het
Tes	T	A	6: 17,100,342 (GRCm38)	C325S	probably benign	Het
Themis	T	C	10: 28,863,394 (GRCm38)	V620A	probably benign	Het
Trim17	T	C	11: 58,971,431 (GRCm38)	S430P	probably damaging	Het
Tubgcp5	T	C	7: 55,795,945 (GRCm38)	I65T	possibly damaging	Het
Vat1l	C	T	8: 114,289,432 (GRCm38)	A354V	probably damaging	Het
Vmn1r170	T	A	7: 23,606,838 (GRCm38)	S222T	probably benign	Het
Vmn2r112	A	T	17: 22,601,232 (GRCm38)	M29L	probably benign	Het
Vmn2r72	T	C	7: 85,751,203 (GRCm38)	I213V	probably benign	Het
Wdr89	T	A	12: 75,632,790 (GRCm38)	Q230L	possibly damaging	Het
Zfp386	A	G	12: 116,059,663 (GRCm38)	T334A	probably benign	Het
Zfp442	T	A	2: 150,409,367 (GRCm38)	H205L	probably benign	Het
Zfyve26	A	T	12: 79,276,302 (GRCm38)	I890N	possibly damaging	Het
Zic1	T	C	9: 91,364,320 (GRCm38)	E233G	probably damaging	Het

Other mutations in Unc13b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Unc13b	APN	4	43,240,285 (GRCm38)	missense	probably damaging	1.00
IGL00832:Unc13b	APN	4	43,258,921 (GRCm38)	missense	probably damaging	1.00
IGL01111:Unc13b	APN	4	43,096,927 (GRCm38)	missense	possibly damaging	0.76
IGL01115:Unc13b	APN	4	43,258,492 (GRCm38)	missense	probably damaging	1.00
IGL01137:Unc13b	APN	4	43,091,291 (GRCm38)	missense	probably damaging	1.00
IGL01637:Unc13b	APN	4	43,241,066 (GRCm38)	missense	probably damaging	1.00
IGL01789:Unc13b	APN	4	43,239,462 (GRCm38)	missense	probably damaging	1.00
IGL01792:Unc13b	APN	4	43,250,218 (GRCm38)	missense	probably damaging	0.99
IGL01877:Unc13b	APN	4	43,249,583 (GRCm38)	critical splice donor site	probably null
IGL01924:Unc13b	APN	4	43,239,385 (GRCm38)	nonsense	probably null
IGL02087:Unc13b	APN	4	43,091,270 (GRCm38)	missense	probably null	1.00
IGL02197:Unc13b	APN	4	43,165,828 (GRCm38)	missense	probably damaging	0.99
IGL02504:Unc13b	APN	4	43,263,031 (GRCm38)	missense	probably damaging	1.00
IGL02659:Unc13b	APN	4	43,235,332 (GRCm38)	missense	probably damaging	1.00
IGL03031:Unc13b	APN	4	43,235,368 (GRCm38)	missense	probably damaging	1.00
IGL03036:Unc13b	APN	4	43,235,249 (GRCm38)	missense	probably damaging	1.00
IGL03209:Unc13b	APN	4	43,239,351 (GRCm38)	missense	probably damaging	0.99
IGL03352:Unc13b	APN	4	43,237,110 (GRCm38)	missense	possibly damaging	0.90
BB006:Unc13b	UTSW	4	43,174,399 (GRCm38)	missense	unknown
BB016:Unc13b	UTSW	4	43,174,399 (GRCm38)	missense	unknown
G1Funyon:Unc13b	UTSW	4	43,263,568 (GRCm38)	missense	probably benign
P0028:Unc13b	UTSW	4	43,256,225 (GRCm38)	missense	probably damaging	1.00
PIT4585001:Unc13b	UTSW	4	43,091,298 (GRCm38)	missense	probably benign	0.03
R0019:Unc13b	UTSW	4	43,096,990 (GRCm38)	missense	possibly damaging	0.58
R0019:Unc13b	UTSW	4	43,096,990 (GRCm38)	missense	possibly damaging	0.58
R0335:Unc13b	UTSW	4	43,236,983 (GRCm38)	missense	possibly damaging	0.95
R0504:Unc13b	UTSW	4	43,263,559 (GRCm38)	missense	probably damaging	0.99
R0631:Unc13b	UTSW	4	43,182,849 (GRCm38)	missense	possibly damaging	0.47
R0748:Unc13b	UTSW	4	43,241,164 (GRCm38)	splice site	probably benign
R1275:Unc13b	UTSW	4	43,235,366 (GRCm38)	missense	probably damaging	1.00
R1293:Unc13b	UTSW	4	43,235,190 (GRCm38)	missense	probably damaging	1.00
R1434:Unc13b	UTSW	4	43,239,385 (GRCm38)	nonsense	probably null
R1552:Unc13b	UTSW	4	43,237,144 (GRCm38)	missense	probably damaging	0.99
R1591:Unc13b	UTSW	4	43,244,747 (GRCm38)	missense	probably damaging	1.00
R1628:Unc13b	UTSW	4	43,263,371 (GRCm38)	missense	probably damaging	1.00
R1740:Unc13b	UTSW	4	43,240,285 (GRCm38)	missense	probably damaging	1.00
R1839:Unc13b	UTSW	4	43,258,308 (GRCm38)	splice site	probably benign
R2045:Unc13b	UTSW	4	43,091,266 (GRCm38)	missense	probably damaging	1.00
R2191:Unc13b	UTSW	4	43,245,566 (GRCm38)	nonsense	probably null
R2259:Unc13b	UTSW	4	43,182,780 (GRCm38)	missense	possibly damaging	0.87
R2307:Unc13b	UTSW	4	43,239,854 (GRCm38)	missense	probably damaging	0.98
R2317:Unc13b	UTSW	4	43,245,514 (GRCm38)	missense	probably damaging	1.00
R2402:Unc13b	UTSW	4	43,095,843 (GRCm38)	missense	probably benign
R2847:Unc13b	UTSW	4	43,180,404 (GRCm38)	missense	probably benign	0.04
R3414:Unc13b	UTSW	4	43,234,658 (GRCm38)	splice site	probably benign
R3436:Unc13b	UTSW	4	43,097,028 (GRCm38)	splice site	probably benign
R3955:Unc13b	UTSW	4	43,256,834 (GRCm38)	missense	probably damaging	1.00
R3957:Unc13b	UTSW	4	43,256,834 (GRCm38)	missense	probably damaging	1.00
R4015:Unc13b	UTSW	4	43,237,801 (GRCm38)	missense	probably damaging	1.00
R4650:Unc13b	UTSW	4	43,261,035 (GRCm38)	missense	probably damaging	0.97
R4836:Unc13b	UTSW	4	43,237,137 (GRCm38)	missense	probably damaging	1.00
R5041:Unc13b	UTSW	4	43,237,836 (GRCm38)	missense	probably benign	0.41
R5413:Unc13b	UTSW	4	43,257,936 (GRCm38)	critical splice donor site	probably null
R5994:Unc13b	UTSW	4	43,172,596 (GRCm38)	intron	probably benign
R6015:Unc13b	UTSW	4	43,177,995 (GRCm38)	nonsense	probably null
R6090:Unc13b	UTSW	4	43,239,306 (GRCm38)	missense	probably damaging	1.00
R6242:Unc13b	UTSW	4	43,165,800 (GRCm38)	missense	possibly damaging	0.92
R6246:Unc13b	UTSW	4	43,216,246 (GRCm38)	missense	probably benign	0.18
R6427:Unc13b	UTSW	4	43,176,966 (GRCm38)	unclassified	probably benign
R6660:Unc13b	UTSW	4	43,177,412 (GRCm38)	unclassified	probably benign
R6670:Unc13b	UTSW	4	43,255,562 (GRCm38)	missense	probably damaging	0.99
R6753:Unc13b	UTSW	4	43,239,331 (GRCm38)	missense	probably damaging	1.00
R6858:Unc13b	UTSW	4	43,165,828 (GRCm38)	missense	possibly damaging	0.85
R6886:Unc13b	UTSW	4	43,170,156 (GRCm38)	intron	probably benign
R6969:Unc13b	UTSW	4	43,263,538 (GRCm38)	missense	possibly damaging	0.94
R6994:Unc13b	UTSW	4	43,173,203 (GRCm38)	intron	probably benign
R6994:Unc13b	UTSW	4	43,171,403 (GRCm38)	intron	probably benign
R7080:Unc13b	UTSW	4	43,171,926 (GRCm38)	missense	unknown
R7117:Unc13b	UTSW	4	43,216,544 (GRCm38)	missense	probably benign	0.33
R7132:Unc13b	UTSW	4	43,215,757 (GRCm38)	missense	probably benign	0.17
R7181:Unc13b	UTSW	4	43,258,893 (GRCm38)	missense	probably damaging	0.99
R7192:Unc13b	UTSW	4	43,258,519 (GRCm38)	missense	probably damaging	1.00
R7246:Unc13b	UTSW	4	43,172,910 (GRCm38)	missense	unknown
R7342:Unc13b	UTSW	4	43,258,703 (GRCm38)	missense	probably damaging	0.99
R7345:Unc13b	UTSW	4	43,173,966 (GRCm38)	missense	unknown
R7355:Unc13b	UTSW	4	43,237,754 (GRCm38)	missense	probably damaging	1.00
R7391:Unc13b	UTSW	4	43,216,459 (GRCm38)	missense	probably benign	0.03
R7419:Unc13b	UTSW	4	43,174,023 (GRCm38)	missense	unknown
R7424:Unc13b	UTSW	4	43,172,235 (GRCm38)	missense	unknown
R7517:Unc13b	UTSW	4	43,215,765 (GRCm38)	missense	probably benign
R7532:Unc13b	UTSW	4	43,249,565 (GRCm38)	missense	probably benign	0.44
R7564:Unc13b	UTSW	4	43,091,258 (GRCm38)	missense	probably damaging	1.00
R7598:Unc13b	UTSW	4	43,263,569 (GRCm38)	missense	probably benign	0.20
R7604:Unc13b	UTSW	4	43,256,776 (GRCm38)	missense	possibly damaging	0.95
R7604:Unc13b	UTSW	4	43,170,102 (GRCm38)	missense	unknown
R7643:Unc13b	UTSW	4	43,216,333 (GRCm38)	missense	probably benign
R7718:Unc13b	UTSW	4	43,173,854 (GRCm38)	missense	unknown
R7735:Unc13b	UTSW	4	43,165,791 (GRCm38)	missense	probably damaging	1.00
R7756:Unc13b	UTSW	4	43,177,312 (GRCm38)	small deletion	probably benign
R7757:Unc13b	UTSW	4	43,177,341 (GRCm38)	small insertion	probably benign
R7757:Unc13b	UTSW	4	43,177,330 (GRCm38)	small insertion	probably benign
R7757:Unc13b	UTSW	4	43,177,312 (GRCm38)	small deletion	probably benign
R7758:Unc13b	UTSW	4	43,177,344 (GRCm38)	small insertion	probably benign
R7758:Unc13b	UTSW	4	43,177,312 (GRCm38)	small insertion	probably benign
R7781:Unc13b	UTSW	4	43,259,546 (GRCm38)	missense	possibly damaging	0.87
R7793:Unc13b	UTSW	4	43,172,737 (GRCm38)	missense	unknown
R7858:Unc13b	UTSW	4	43,176,285 (GRCm38)	missense	unknown
R7867:Unc13b	UTSW	4	43,232,573 (GRCm38)	nonsense	probably null
R7897:Unc13b	UTSW	4	43,171,860 (GRCm38)	missense	unknown
R7904:Unc13b	UTSW	4	43,217,075 (GRCm38)	missense	probably benign
R7929:Unc13b	UTSW	4	43,174,399 (GRCm38)	missense	unknown
R7984:Unc13b	UTSW	4	43,173,973 (GRCm38)	missense	unknown
R8069:Unc13b	UTSW	4	43,177,597 (GRCm38)	missense	unknown
R8101:Unc13b	UTSW	4	43,239,918 (GRCm38)	missense	probably benign	0.08
R8246:Unc13b	UTSW	4	43,175,954 (GRCm38)	missense	unknown
R8289:Unc13b	UTSW	4	43,172,524 (GRCm38)	nonsense	probably null
R8301:Unc13b	UTSW	4	43,263,568 (GRCm38)	missense	probably benign
R8397:Unc13b	UTSW	4	43,217,290 (GRCm38)	missense	probably benign	0.12
R8421:Unc13b	UTSW	4	43,178,304 (GRCm38)	missense	unknown
R8738:Unc13b	UTSW	4	43,177,564 (GRCm38)	missense	unknown
R8746:Unc13b	UTSW	4	43,176,120 (GRCm38)	missense	unknown
R8766:Unc13b	UTSW	4	43,174,722 (GRCm38)	missense	unknown
R8825:Unc13b	UTSW	4	43,237,683 (GRCm38)	splice site	probably benign
R8834:Unc13b	UTSW	4	43,175,954 (GRCm38)	missense	unknown
R8862:Unc13b	UTSW	4	43,235,207 (GRCm38)	missense	probably damaging	1.00
R8864:Unc13b	UTSW	4	43,174,724 (GRCm38)	missense	unknown
R8889:Unc13b	UTSW	4	43,176,484 (GRCm38)	missense	unknown
R8892:Unc13b	UTSW	4	43,176,484 (GRCm38)	missense	unknown
R8904:Unc13b	UTSW	4	43,178,531 (GRCm38)	intron	probably benign
R9089:Unc13b	UTSW	4	43,095,847 (GRCm38)	missense	probably damaging	1.00
R9144:Unc13b	UTSW	4	43,173,649 (GRCm38)	missense	unknown
R9149:Unc13b	UTSW	4	43,176,186 (GRCm38)	missense	unknown
R9173:Unc13b	UTSW	4	43,177,421 (GRCm38)	missense	unknown
R9200:Unc13b	UTSW	4	43,257,352 (GRCm38)	missense	possibly damaging	0.50
R9232:Unc13b	UTSW	4	43,240,321 (GRCm38)	missense	probably benign	0.03
R9320:Unc13b	UTSW	4	43,171,044 (GRCm38)	missense	unknown
R9335:Unc13b	UTSW	4	43,255,551 (GRCm38)	missense	probably damaging	0.99
R9335:Unc13b	UTSW	4	43,216,123 (GRCm38)	missense	possibly damaging	0.86
R9352:Unc13b	UTSW	4	43,177,313 (GRCm38)	nonsense	probably null
R9352:Unc13b	UTSW	4	43,177,312 (GRCm38)	small insertion	probably benign
R9378:Unc13b	UTSW	4	43,173,282 (GRCm38)	missense	unknown
R9382:Unc13b	UTSW	4	43,172,512 (GRCm38)	missense	unknown
R9569:Unc13b	UTSW	4	43,177,312 (GRCm38)	small deletion	probably benign
R9622:Unc13b	UTSW	4	43,172,513 (GRCm38)	missense
R9687:Unc13b	UTSW	4	43,174,920 (GRCm38)	missense	unknown
R9704:Unc13b	UTSW	4	43,237,102 (GRCm38)	missense	probably benign	0.31
R9721:Unc13b	UTSW	4	43,101,869 (GRCm38)	missense	probably benign
R9753:Unc13b	UTSW	4	43,182,842 (GRCm38)	nonsense	probably null
RF016:Unc13b	UTSW	4	43,177,350 (GRCm38)	small insertion	probably benign
RF016:Unc13b	UTSW	4	43,177,347 (GRCm38)	small insertion	probably benign
RF041:Unc13b	UTSW	4	43,177,338 (GRCm38)	small insertion	probably benign
RF056:Unc13b	UTSW	4	43,177,359 (GRCm38)	small insertion	probably benign
Z1176:Unc13b	UTSW	4	43,177,764 (GRCm38)	missense	unknown
Z1176:Unc13b	UTSW	4	43,177,191 (GRCm38)	missense	unknown
Z1176:Unc13b	UTSW	4	43,171,419 (GRCm38)	missense	unknown
Z1176:Unc13b	UTSW	4	43,261,043 (GRCm38)	missense	probably benign	0.11
Z1177:Unc13b	UTSW	4	43,173,669 (GRCm38)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGCCAAAGATACTGTGGAGC -3'
(R):5'- GCTACTGAGCTGTGAAAACTCTG -3'

Sequencing Primer
(F):5'- CCAAAGATACTGTGGAGCGTTGTG -3'
(R):5'- AACAGTGCTATGACTGTCTGTGAGAG -3'

Posted On

2022-03-25