Incidental Mutation 'R0750:Brd4'
ID 70292
Institutional Source Beutler Lab
Gene Symbol Brd4
Ensembl Gene ENSMUSG00000024002
Gene Name bromodomain containing 4
Synonyms WI-11513, HUNK1, MCAP
MMRRC Submission 038930-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R0750 (G1)
Quality Score 225
Status Not validated
Chromosome 17
Chromosomal Location 32415248-32503696 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 32439226 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamic Acid to Glycine at position 418 (E418G)
Ref Sequence ENSEMBL: ENSMUSP00000115163 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000003726] [ENSMUST00000114475] [ENSMUST00000119123] [ENSMUST00000120276] [ENSMUST00000121285] [ENSMUST00000125899] [ENSMUST00000127893]
AlphaFold Q9ESU6
Predicted Effect unknown
Transcript: ENSMUST00000003726
AA Change: E419G
SMART Domains Protein: ENSMUSP00000003726
Gene: ENSMUSG00000024002
AA Change: E419G

DomainStartEndE-ValueType
low complexity region 23 54 N/A INTRINSIC
BROMO 56 166 1e-52 SMART
low complexity region 176 191 N/A INTRINSIC
low complexity region 198 224 N/A INTRINSIC
low complexity region 237 275 N/A INTRINSIC
low complexity region 295 305 N/A INTRINSIC
low complexity region 329 339 N/A INTRINSIC
BROMO 352 461 1.2e-48 SMART
coiled coil region 504 570 N/A INTRINSIC
Pfam:BET 611 675 6.4e-33 PFAM
low complexity region 701 722 N/A INTRINSIC
low complexity region 746 796 N/A INTRINSIC
low complexity region 828 854 N/A INTRINSIC
low complexity region 890 926 N/A INTRINSIC
low complexity region 929 939 N/A INTRINSIC
low complexity region 953 1005 N/A INTRINSIC
low complexity region 1013 1042 N/A INTRINSIC
low complexity region 1086 1100 N/A INTRINSIC
low complexity region 1105 1120 N/A INTRINSIC
low complexity region 1135 1147 N/A INTRINSIC
low complexity region 1201 1212 N/A INTRINSIC
low complexity region 1248 1260 N/A INTRINSIC
coiled coil region 1261 1345 N/A INTRINSIC
Pfam:BRD4_CDT 1358 1400 3.8e-24 PFAM
Predicted Effect unknown
Transcript: ENSMUST00000114475
AA Change: E418G
SMART Domains Protein: ENSMUSP00000110119
Gene: ENSMUSG00000024002
AA Change: E418G

DomainStartEndE-ValueType
low complexity region 23 54 N/A INTRINSIC
BROMO 56 166 1.67e-50 SMART
low complexity region 197 223 N/A INTRINSIC
low complexity region 236 274 N/A INTRINSIC
low complexity region 294 304 N/A INTRINSIC
low complexity region 328 338 N/A INTRINSIC
BROMO 351 460 1.81e-46 SMART
coiled coil region 503 569 N/A INTRINSIC
PDB:2JNS|A 606 683 3e-46 PDB
low complexity region 700 720 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000119123
AA Change: E418G
SMART Domains Protein: ENSMUSP00000113197
Gene: ENSMUSG00000024002
AA Change: E418G

DomainStartEndE-ValueType
low complexity region 23 54 N/A INTRINSIC
BROMO 56 166 1.67e-50 SMART
low complexity region 197 223 N/A INTRINSIC
low complexity region 236 274 N/A INTRINSIC
low complexity region 294 304 N/A INTRINSIC
low complexity region 328 338 N/A INTRINSIC
BROMO 351 460 1.81e-46 SMART
coiled coil region 503 569 N/A INTRINSIC
PDB:2JNS|A 606 683 6e-46 PDB
low complexity region 700 721 N/A INTRINSIC
low complexity region 745 795 N/A INTRINSIC
low complexity region 827 853 N/A INTRINSIC
low complexity region 889 925 N/A INTRINSIC
low complexity region 928 938 N/A INTRINSIC
low complexity region 952 1004 N/A INTRINSIC
low complexity region 1012 1041 N/A INTRINSIC
low complexity region 1085 1099 N/A INTRINSIC
low complexity region 1104 1119 N/A INTRINSIC
low complexity region 1134 1146 N/A INTRINSIC
low complexity region 1200 1211 N/A INTRINSIC
low complexity region 1247 1259 N/A INTRINSIC
coiled coil region 1260 1344 N/A INTRINSIC
low complexity region 1361 1381 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000120276
AA Change: E418G
SMART Domains Protein: ENSMUSP00000112474
Gene: ENSMUSG00000024002
AA Change: E418G

DomainStartEndE-ValueType
low complexity region 23 54 N/A INTRINSIC
BROMO 56 166 1.67e-50 SMART
low complexity region 197 223 N/A INTRINSIC
low complexity region 236 274 N/A INTRINSIC
low complexity region 294 304 N/A INTRINSIC
low complexity region 328 338 N/A INTRINSIC
BROMO 351 460 1.81e-46 SMART
coiled coil region 503 569 N/A INTRINSIC
PDB:2JNS|A 606 683 3e-46 PDB
low complexity region 700 721 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000121285
AA Change: E418G
SMART Domains Protein: ENSMUSP00000113070
Gene: ENSMUSG00000024002
AA Change: E418G

DomainStartEndE-ValueType
low complexity region 23 54 N/A INTRINSIC
BROMO 56 166 1.67e-50 SMART
low complexity region 197 223 N/A INTRINSIC
low complexity region 236 274 N/A INTRINSIC
low complexity region 294 304 N/A INTRINSIC
low complexity region 328 338 N/A INTRINSIC
BROMO 351 460 1.81e-46 SMART
coiled coil region 503 569 N/A INTRINSIC
PDB:2JNS|A 606 683 6e-46 PDB
low complexity region 700 721 N/A INTRINSIC
low complexity region 745 795 N/A INTRINSIC
low complexity region 827 853 N/A INTRINSIC
low complexity region 889 925 N/A INTRINSIC
low complexity region 928 938 N/A INTRINSIC
low complexity region 952 1004 N/A INTRINSIC
low complexity region 1012 1041 N/A INTRINSIC
low complexity region 1085 1099 N/A INTRINSIC
low complexity region 1104 1119 N/A INTRINSIC
low complexity region 1134 1146 N/A INTRINSIC
low complexity region 1200 1211 N/A INTRINSIC
low complexity region 1247 1259 N/A INTRINSIC
coiled coil region 1260 1344 N/A INTRINSIC
low complexity region 1361 1381 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000125899
SMART Domains Protein: ENSMUSP00000115277
Gene: ENSMUSG00000024002

DomainStartEndE-ValueType
low complexity region 23 54 N/A INTRINSIC
BROMO 56 144 5.02e-31 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000127893
AA Change: E418G

PolyPhen 2 Score 0.086 (Sensitivity: 0.93; Specificity: 0.85)
SMART Domains Protein: ENSMUSP00000115163
Gene: ENSMUSG00000024002
AA Change: E418G

DomainStartEndE-ValueType
low complexity region 23 54 N/A INTRINSIC
BROMO 56 166 1.67e-50 SMART
low complexity region 197 223 N/A INTRINSIC
low complexity region 236 274 N/A INTRINSIC
low complexity region 294 304 N/A INTRINSIC
low complexity region 328 338 N/A INTRINSIC
BROMO 351 460 1.81e-46 SMART
coiled coil region 503 531 N/A INTRINSIC
low complexity region 536 557 N/A INTRINSIC
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.6%
  • 10x: 97.0%
  • 20x: 93.3%
Validation Efficiency
MGI Phenotype FUNCTION: This gene was temporarily named bromodomain-containing 5 (Brd5) and was renamed bromodomain-containing 4 (Brd4). [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a gene-trap null mutation die soon after implantation. Heterozygotes exhibit impaired pre- and postnatal growth, head malformations, lack of subcutaneous fat, cataracts, and abnormal liver cells. [provided by MGI curators]
Allele List at MGI

All alleles(161) : Targeted(1) Gene trapped(160)

Other mutations in this stock
Total: 23 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Agrn A T 4: 156,251,394 (GRCm39) L1974* probably null Het
Brip1 G A 11: 85,952,325 (GRCm39) S1152L possibly damaging Het
Btrc T G 19: 45,491,585 (GRCm39) F81C probably damaging Het
Cep85l A G 10: 53,157,642 (GRCm39) L585P probably damaging Het
Cfap46 T G 7: 139,234,586 (GRCm39) E671D probably damaging Het
Dsg1a T C 18: 20,473,210 (GRCm39) L761P probably benign Het
Ece2 G T 16: 20,451,800 (GRCm39) V396L probably benign Het
Garin4 G A 1: 190,896,682 (GRCm39) probably benign Het
Hs6st3 CGGAGGAGGAGGAGGAGGA CGGAGGAGGAGGAGGA 14: 119,376,119 (GRCm39) probably benign Het
Id2 A G 12: 25,145,670 (GRCm39) S114P probably damaging Het
Igf1r T C 7: 67,861,839 (GRCm39) F1133S probably damaging Het
Izumo1 T C 7: 45,275,707 (GRCm39) probably null Het
Krt35 A G 11: 99,986,979 (GRCm39) S12P possibly damaging Het
Or5a1 T C 19: 12,098,077 (GRCm39) probably null Het
Pkdrej G T 15: 85,702,275 (GRCm39) D1220E probably benign Het
Pramel32 A G 4: 88,545,905 (GRCm39) F479S probably benign Het
Sema3a G A 5: 13,607,092 (GRCm39) probably null Het
Tmed6 T C 8: 107,788,401 (GRCm39) Y182C possibly damaging Het
Tmem174 G T 13: 98,773,787 (GRCm39) N14K probably damaging Het
Tmem87b T C 2: 128,660,356 (GRCm39) L33P possibly damaging Het
Vmn1r16 T C 6: 57,299,812 (GRCm39) Y270C probably benign Het
Vps37d A T 5: 135,103,294 (GRCm39) L116Q possibly damaging Het
Zfp592 A G 7: 80,674,493 (GRCm39) S486G probably benign Het
Other mutations in Brd4
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01736:Brd4 APN 17 32,417,649 (GRCm39) splice site probably benign
IGL01758:Brd4 APN 17 32,431,803 (GRCm39) unclassified probably benign
IGL02750:Brd4 APN 17 32,417,353 (GRCm39) unclassified probably benign
IGL03066:Brd4 APN 17 32,418,062 (GRCm39) intron probably benign
IGL03338:Brd4 APN 17 32,432,046 (GRCm39) missense probably damaging 1.00
Admirable UTSW 17 32,444,557 (GRCm39) missense unknown
H8562:Brd4 UTSW 17 32,448,377 (GRCm39) splice site probably benign
P0035:Brd4 UTSW 17 32,431,812 (GRCm39) critical splice donor site probably null
R0243:Brd4 UTSW 17 32,443,097 (GRCm39) missense probably benign 0.15
R0281:Brd4 UTSW 17 32,432,514 (GRCm39) unclassified probably benign
R0331:Brd4 UTSW 17 32,421,489 (GRCm39) missense probably benign 0.01
R0722:Brd4 UTSW 17 32,431,956 (GRCm39) missense possibly damaging 0.76
R1544:Brd4 UTSW 17 32,417,646 (GRCm39) splice site probably benign
R1920:Brd4 UTSW 17 32,417,060 (GRCm39) unclassified probably benign
R1922:Brd4 UTSW 17 32,417,060 (GRCm39) unclassified probably benign
R1957:Brd4 UTSW 17 32,440,340 (GRCm39) missense possibly damaging 0.50
R2240:Brd4 UTSW 17 32,432,613 (GRCm39) unclassified probably benign
R2316:Brd4 UTSW 17 32,431,884 (GRCm39) missense probably benign 0.03
R2333:Brd4 UTSW 17 32,440,431 (GRCm39) missense probably damaging 0.97
R3809:Brd4 UTSW 17 32,430,244 (GRCm39) missense possibly damaging 0.72
R4273:Brd4 UTSW 17 32,433,756 (GRCm39) missense probably benign
R4595:Brd4 UTSW 17 32,417,896 (GRCm39) missense probably damaging 0.97
R4854:Brd4 UTSW 17 32,439,211 (GRCm39) missense probably damaging 0.96
R4923:Brd4 UTSW 17 32,418,214 (GRCm39) missense probably benign 0.38
R5014:Brd4 UTSW 17 32,417,372 (GRCm39) unclassified probably benign
R5757:Brd4 UTSW 17 32,420,272 (GRCm39) unclassified probably benign
R5979:Brd4 UTSW 17 32,417,700 (GRCm39) missense probably benign 0.32
R6212:Brd4 UTSW 17 32,421,423 (GRCm39) missense probably damaging 0.98
R6394:Brd4 UTSW 17 32,443,121 (GRCm39) nonsense probably null
R6643:Brd4 UTSW 17 32,417,470 (GRCm39) missense unknown
R7024:Brd4 UTSW 17 32,440,884 (GRCm39) utr 3 prime probably benign
R7033:Brd4 UTSW 17 32,417,989 (GRCm39) missense probably benign 0.13
R7220:Brd4 UTSW 17 32,444,557 (GRCm39) missense unknown
R7682:Brd4 UTSW 17 32,420,134 (GRCm39) missense unknown
R7731:Brd4 UTSW 17 32,430,198 (GRCm39) missense possibly damaging 0.73
R7732:Brd4 UTSW 17 32,440,386 (GRCm39) missense unknown
R7750:Brd4 UTSW 17 32,432,521 (GRCm39) missense unknown
R7756:Brd4 UTSW 17 32,417,956 (GRCm39) missense unknown
R7758:Brd4 UTSW 17 32,417,956 (GRCm39) missense unknown
R7779:Brd4 UTSW 17 32,431,910 (GRCm39) missense probably benign 0.03
R8214:Brd4 UTSW 17 32,431,921 (GRCm39) missense probably benign 0.19
R8405:Brd4 UTSW 17 32,448,505 (GRCm39) missense unknown
R9675:Brd4 UTSW 17 32,433,786 (GRCm39) missense unknown
X0064:Brd4 UTSW 17 32,420,101 (GRCm39) unclassified probably benign
Predicted Primers PCR Primer
(F):5'- CTCCAGGTTGTTGAAGGGGCAG -3'
(R):5'- TCTCATGCCGAGAACCGGAGTG -3'

Sequencing Primer
(F):5'- acaaacaaacaaacaaacaaacaaac -3'
(R):5'- cacacacacacacacacac -3'
Posted On 2013-09-30