Mutagenetix > Incidental Mutation

Incidental Mutation 'R0751:Aspm'

70297

Institutional Source

Beutler Lab

Gene Symbol

Aspm

Ensembl Gene

ENSMUSG00000033952

Gene Name

abnormal spindle microtubule assembly

Synonyms

Aspm, Sha1, MCPH5, D330028K02Rik, Calmbp1

MMRRC Submission

038931-MU

Accession Numbers

Genbank: NM_009791; MGI: 1334448

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0751 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

139454772-139494091 bp(+) (GRCm38)

Type of Mutation

splice site

DNA Base Change (assembly)

A to T at 139456898 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000142880 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039867] [ENSMUST00000053364] [ENSMUST00000200083]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000039867

SMART Domains

Protein: ENSMUSP00000045570
Gene: ENSMUSG00000033964

Domain	Start	End	E-Value	Type
low complexity region	70	81	N/A	INTRINSIC
BTB	89	183	7.06e-16	SMART
ZnF_C2H2	208	231	3.78e-1	SMART
low complexity region	234	245	N/A	INTRINSIC
low complexity region	265	279	N/A	INTRINSIC
low complexity region	301	327	N/A	INTRINSIC
ZnF_C2H2	360	382	4.17e-3	SMART
ZnF_C2H2	388	410	8.34e-3	SMART
ZnF_C2H2	421	444	2.67e-1	SMART
ZnF_C2H2	462	484	1.72e-4	SMART
ZnF_C2H2	490	513	1.41e0	SMART
ZnF_C2H2	517	540	1.12e-3	SMART
ZnF_C2H2	546	568	1.36e-2	SMART
ZnF_C2H2	574	596	2.91e-2	SMART
ZnF_C2H2	602	624	7.37e-4	SMART
ZnF_C2H2	630	653	3.39e-3	SMART
ZnF_C2H2	667	689	2.75e-3	SMART
ZnF_C2H2	695	717	3.16e-3	SMART
ZnF_C2H2	723	746	3.34e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000053364

SMART Domains

Protein: ENSMUSP00000059159
Gene: ENSMUSG00000033952

Domain	Start	End	E-Value	Type
Pfam:ASH	29	126	8.9e-35	PFAM
low complexity region	855	861	N/A	INTRINSIC
CH	890	1022	2.04e0	SMART
CH	1080	1224	5.56e-9	SMART
IQ	1233	1255	7.57e0	SMART
IQ	1259	1281	1.12e1	SMART
IQ	1282	1304	3.73e-1	SMART
IQ	1314	1336	2.41e-4	SMART
IQ	1360	1382	2.12e1	SMART
IQ	1387	1408	7.61e1	SMART
IQ	1409	1431	6.97e0	SMART
IQ	1432	1452	1.44e1	SMART
IQ	1453	1475	1.15e-1	SMART
IQ	1476	1495	1.66e2	SMART
IQ	1503	1525	1.65e-2	SMART
IQ	1526	1548	1.32e1	SMART
IQ	1549	1571	1.48e1	SMART
IQ	1572	1594	2.5e1	SMART
IQ	1599	1621	2.58e-4	SMART
IQ	1622	1644	6.7e-3	SMART
IQ	1645	1667	4.25e1	SMART
IQ	1668	1694	1.03e2	SMART
IQ	1695	1717	2.33e-2	SMART
IQ	1718	1740	7.79e0	SMART
IQ	1741	1763	1.57e2	SMART
IQ	1768	1790	2.68e-2	SMART
IQ	1791	1813	5.83e-3	SMART
IQ	1814	1836	5.93e1	SMART
IQ	1841	1863	1.92e-3	SMART
IQ	1864	1886	3.79e-2	SMART
IQ	1914	1936	4.11e0	SMART
IQ	1937	1959	1.87e-1	SMART
IQ	1960	1982	6.27e1	SMART
IQ	1987	2009	8.25e-3	SMART
IQ	2010	2032	5.73e0	SMART
IQ	2060	2082	1.39e0	SMART
IQ	2083	2105	4.62e1	SMART
IQ	2133	2155	5.58e0	SMART
IQ	2156	2178	7.07e-2	SMART
IQ	2206	2228	1.18e-3	SMART
IQ	2229	2251	4.59e0	SMART
IQ	2278	2300	1.85e-5	SMART
IQ	2301	2323	8.13e-2	SMART
IQ	2342	2364	9.62e-4	SMART
IQ	2365	2387	4.12e-3	SMART
IQ	2415	2437	7.58e-2	SMART
IQ	2438	2460	2.6e0	SMART
IQ	2490	2512	1.68e-3	SMART
IQ	2513	2535	8.51e1	SMART
IQ	2560	2582	2.14e-1	SMART
IQ	2601	2623	8.46e0	SMART
IQ	2647	2669	1.15e1	SMART
IQ	2673	2695	1.95e-4	SMART
IQ	2696	2718	4.13e1	SMART
IQ	2723	2745	1.02e-2	SMART
IQ	2761	2783	3.14e2	SMART
IQ	2784	2806	1e1	SMART
IQ	2825	2847	2.43e0	SMART
IQ	2848	2870	4.6e-1	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000197096

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199998

Predicted Effect

probably benign
Transcript: ENSMUST00000200083

SMART Domains

Protein: ENSMUSP00000142880
Gene: ENSMUSG00000033952

Domain	Start	End	E-Value	Type
low complexity region	855	861	N/A	INTRINSIC
CH	890	1022	2.04e0	SMART
CH	1080	1224	5.56e-9	SMART
IQ	1233	1255	7.57e0	SMART
IQ	1259	1281	1.12e1	SMART
IQ	1282	1304	3.73e-1	SMART
IQ	1314	1336	1.25e1	SMART
IQ	1337	1358	2.96e1	SMART
IQ	1382	1404	1.15e1	SMART
IQ	1408	1430	1.95e-4	SMART
IQ	1431	1453	4.13e1	SMART
IQ	1458	1480	1.02e-2	SMART
IQ	1496	1518	3.14e2	SMART
IQ	1519	1541	1e1	SMART
IQ	1560	1582	2.43e0	SMART
IQ	1583	1605	4.6e-1	SMART

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.2%
20x: 94.4%

Validation Efficiency

99% (73/74)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]
PHENOTYPE: Mice homozygous for protein-truncating gene trap mutations of this gene exhibit decreased body weight, microcephaly, a severe reduction in brain, testis and ovary weight, oligozoospermia and asthenospermia, and reduced fertility in both sexes. [provided by MGI curators]

Allele List at MGI

All alleles(9) : Targeted, other(2) Gene trapped(7)

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ahdc1	T	A	4: 133,065,396 (GRCm38)	M1316K	probably benign	Het
Alox12	T	C	11: 70,246,950 (GRCm38)	I455V	probably benign	Het
Ankrd28	A	G	14: 31,764,268 (GRCm38)	L89P	probably damaging	Het
Aqp9	A	T	9: 71,138,205 (GRCm38)	C41S	probably damaging	Het
Arhgap17	T	C	7: 123,314,690 (GRCm38)	Y199C	probably damaging	Het
Cacfd1	T	C	2: 27,018,981 (GRCm38)		probably null	Het
Cd33	T	C	7: 43,532,121 (GRCm38)	D205G	probably damaging	Het
Chadl	T	C	15: 81,693,057 (GRCm38)	S198G	probably benign	Het
Chtf8	A	G	8: 106,886,477 (GRCm38)		probably null	Het
Clec4a4	G	T	6: 123,012,712 (GRCm38)	W104L	probably benign	Het
Clock	A	T	5: 76,229,361 (GRCm38)	I696K	possibly damaging	Het
Crtc2	T	A	3: 90,262,633 (GRCm38)	Y445*	probably null	Het
Dapk1	A	T	13: 60,696,298 (GRCm38)	I44F	probably damaging	Het
Dcbld2	T	A	16: 58,449,841 (GRCm38)		probably null	Het
Derl2	T	C	11: 71,014,547 (GRCm38)		probably null	Het
Dnah7c	A	G	1: 46,465,905 (GRCm38)	T154A	probably benign	Het
Dnmt3b	T	A	2: 153,674,842 (GRCm38)		probably null	Het
Dusp3	A	T	11: 101,981,728 (GRCm38)	S106T	probably benign	Het
Eftud2	A	G	11: 102,839,253 (GRCm38)	V897A	probably damaging	Het
Eif3l	T	A	15: 79,075,766 (GRCm38)		probably null	Het
Fbxo33	A	C	12: 59,219,092 (GRCm38)	F130V	probably damaging	Het
Ffar3	T	A	7: 30,855,104 (GRCm38)	N264Y	probably damaging	Het
Fig4	T	C	10: 41,272,982 (GRCm38)	D158G	probably damaging	Het
Fyco1	A	G	9: 123,819,153 (GRCm38)	F1239L	probably damaging	Het
Gabra2	A	G	5: 71,092,099 (GRCm38)		probably benign	Het
Gabra6	C	A	11: 42,315,017 (GRCm38)	R336S	probably benign	Het
Gm9268	A	G	7: 43,047,409 (GRCm38)	Y630C	probably damaging	Het
Hkdc1	T	C	10: 62,398,673 (GRCm38)	D581G	probably damaging	Het
Iqgap1	A	G	7: 80,725,573 (GRCm38)		probably benign	Het
Larp4b	T	C	13: 9,166,309 (GRCm38)		probably benign	Het
Lcp1	A	T	14: 75,199,387 (GRCm38)	M58L	probably benign	Het
Lrrc8a	T	C	2: 30,256,350 (GRCm38)	V392A	possibly damaging	Het
Mavs	A	T	2: 131,246,764 (GRCm38)	Y496F	probably damaging	Het
Mpi	A	T	9: 57,550,614 (GRCm38)	S102T	probably damaging	Het
Mroh9	G	A	1: 163,066,124 (GRCm38)	R161W	possibly damaging	Het
Myo1h	A	T	5: 114,320,686 (GRCm38)	S161C	probably damaging	Het
Napg	T	G	18: 62,994,338 (GRCm38)	H204Q	probably benign	Het
Nelfcd	C	A	2: 174,423,014 (GRCm38)	A182D	probably benign	Het
Ntsr2	G	T	12: 16,654,030 (GRCm38)	K91N	probably damaging	Het
Obscn	A	G	11: 59,081,819 (GRCm38)	S2134P	probably damaging	Het
Ogfod2	G	A	5: 124,113,476 (GRCm38)		probably benign	Het
Olfr385	G	T	11: 73,589,144 (GRCm38)	T198K	probably benign	Het
Olfr525	G	A	7: 140,323,325 (GRCm38)	V209I	probably benign	Het
Pcdha8	T	C	18: 36,994,070 (GRCm38)	V535A	probably damaging	Het
Pdlim2	C	T	14: 70,164,779 (GRCm38)	R296H	probably damaging	Het
Pik3r1	T	C	13: 101,686,358 (GRCm38)		probably null	Het
Pimreg	C	A	11: 72,043,113 (GRCm38)	Q22K	probably benign	Het
Pld5	A	G	1: 176,044,896 (GRCm38)	I225T	probably damaging	Het
Plxnc1	T	C	10: 94,831,333 (GRCm38)		probably benign	Het
Ppip5k2	A	T	1: 97,749,652 (GRCm38)	C306*	probably null	Het
Ptprc	A	G	1: 138,092,930 (GRCm38)	Y588H	probably damaging	Het
Rac2	T	G	15: 78,565,945 (GRCm38)	D65A	possibly damaging	Het
Rgl3	A	G	9: 21,977,380 (GRCm38)		probably null	Het
Serpinb1a	T	C	13: 32,843,216 (GRCm38)	K248E	probably benign	Het
Serpinb9e	A	C	13: 33,259,774 (GRCm38)	E259A	probably benign	Het
Slc12a4	A	T	8: 105,951,900 (GRCm38)	V266E	probably damaging	Het
Slc8b1	A	G	5: 120,524,195 (GRCm38)		probably benign	Het
Smim4	G	T	14: 31,088,996 (GRCm38)		probably benign	Het
Spink6	T	C	18: 44,071,538 (GRCm38)		probably benign	Het
Spta1	G	A	1: 174,184,690 (GRCm38)	R354H	probably damaging	Het
Ssb	T	A	2: 69,870,565 (GRCm38)	S330T	probably benign	Het
Stard9	G	T	2: 120,697,485 (GRCm38)	V1408F	probably benign	Het
Sumf2	A	T	5: 129,850,005 (GRCm38)	T61S	probably benign	Het
Sypl2	T	C	3: 108,216,756 (GRCm38)	T157A	probably damaging	Het
Tgfbr3	A	T	5: 107,139,883 (GRCm38)	D483E	probably damaging	Het
Tnrc6a	A	G	7: 123,170,340 (GRCm38)	N451S	possibly damaging	Het
Tradd	T	C	8: 105,259,771 (GRCm38)	E123G	probably damaging	Het
Trim36	T	C	18: 46,196,251 (GRCm38)	T41A	probably damaging	Het
Ttc30a2	C	T	2: 75,978,031 (GRCm38)	A46T	probably damaging	Het
Ttll7	C	T	3: 146,939,991 (GRCm38)	P535S	probably damaging	Het
Ubr4	C	T	4: 139,437,198 (GRCm38)		probably benign	Het
Vmn1r195	A	G	13: 22,279,011 (GRCm38)	Y217C	probably damaging	Het
Vmn2r63	A	C	7: 42,928,035 (GRCm38)	F360V	probably damaging	Het
Vmn2r78	G	A	7: 86,954,380 (GRCm38)	V589M	possibly damaging	Het
Vrk2	A	G	11: 26,483,331 (GRCm38)		probably benign	Het

Other mutations in Aspm

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00489:Aspm	APN	1	139,478,691 (GRCm38)	missense	probably damaging	1.00
IGL00594:Aspm	APN	1	139,487,422 (GRCm38)	splice site	probably benign
IGL00808:Aspm	APN	1	139,461,476 (GRCm38)	missense	probably benign	0.03
IGL00897:Aspm	APN	1	139,477,407 (GRCm38)	missense	probably damaging	0.98
IGL01024:Aspm	APN	1	139,478,124 (GRCm38)	missense	possibly damaging	0.66
IGL01410:Aspm	APN	1	139,482,444 (GRCm38)	missense	probably benign	0.25
IGL01588:Aspm	APN	1	139,478,162 (GRCm38)	missense	probably benign	0.11
IGL01610:Aspm	APN	1	139,489,670 (GRCm38)	nonsense	probably null
IGL01633:Aspm	APN	1	139,480,836 (GRCm38)	missense	possibly damaging	0.93
IGL01982:Aspm	APN	1	139,491,588 (GRCm38)	missense	probably benign	0.12
IGL02429:Aspm	APN	1	139,479,810 (GRCm38)	missense	probably benign	0.27
IGL02468:Aspm	APN	1	139,480,950 (GRCm38)	missense	probably damaging	1.00
IGL02519:Aspm	APN	1	139,461,927 (GRCm38)	splice site	probably benign
IGL02526:Aspm	APN	1	139,489,719 (GRCm38)	missense	probably benign	0.03
IGL02716:Aspm	APN	1	139,479,687 (GRCm38)	missense	probably damaging	1.00
IGL02876:Aspm	APN	1	139,473,653 (GRCm38)	missense	probably damaging	1.00
IGL02953:Aspm	APN	1	139,457,419 (GRCm38)	missense	probably benign	0.01
IGL03275:Aspm	APN	1	139,487,295 (GRCm38)	missense	probably damaging	1.00
Stemware	UTSW	1	139,477,459 (GRCm38)	nonsense	probably null
3-1:Aspm	UTSW	1	139,457,541 (GRCm38)	missense	probably benign
R0016:Aspm	UTSW	1	139,479,544 (GRCm38)	missense	probably benign	0.01
R0016:Aspm	UTSW	1	139,479,544 (GRCm38)	missense	probably benign	0.01
R0106:Aspm	UTSW	1	139,476,876 (GRCm38)	missense	probably benign	0.02
R0106:Aspm	UTSW	1	139,476,876 (GRCm38)	missense	probably benign	0.02
R0140:Aspm	UTSW	1	139,480,641 (GRCm38)	missense	probably benign	0.00
R0195:Aspm	UTSW	1	139,479,135 (GRCm38)	missense	probably damaging	1.00
R0217:Aspm	UTSW	1	139,457,880 (GRCm38)	missense	possibly damaging	0.46
R0276:Aspm	UTSW	1	139,478,471 (GRCm38)	missense	possibly damaging	0.95
R0309:Aspm	UTSW	1	139,482,511 (GRCm38)	splice site	probably benign
R0466:Aspm	UTSW	1	139,477,901 (GRCm38)	missense	probably damaging	1.00
R0520:Aspm	UTSW	1	139,478,820 (GRCm38)	missense	possibly damaging	0.51
R0615:Aspm	UTSW	1	139,487,289 (GRCm38)	missense	probably damaging	1.00
R0626:Aspm	UTSW	1	139,491,601 (GRCm38)	missense	probably damaging	1.00
R0660:Aspm	UTSW	1	139,457,764 (GRCm38)	missense	probably benign	0.03
R0830:Aspm	UTSW	1	139,474,254 (GRCm38)	missense	probably damaging	0.99
R1109:Aspm	UTSW	1	139,456,758 (GRCm38)	missense	probably damaging	0.99
R1114:Aspm	UTSW	1	139,461,924 (GRCm38)	splice site	probably benign
R1130:Aspm	UTSW	1	139,477,834 (GRCm38)	missense	possibly damaging	0.90
R1298:Aspm	UTSW	1	139,457,419 (GRCm38)	missense	probably benign	0.01
R1386:Aspm	UTSW	1	139,478,972 (GRCm38)	missense	possibly damaging	0.80
R1386:Aspm	UTSW	1	139,457,623 (GRCm38)	missense	probably benign	0.03
R1557:Aspm	UTSW	1	139,468,668 (GRCm38)	missense	probably benign	0.01
R1625:Aspm	UTSW	1	139,481,039 (GRCm38)	missense	probably benign	0.01
R1728:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1729:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1730:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1733:Aspm	UTSW	1	139,457,117 (GRCm38)	missense	probably benign	0.27
R1739:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1762:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1783:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1784:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1785:Aspm	UTSW	1	139,473,574 (GRCm38)	missense	probably benign
R1793:Aspm	UTSW	1	139,457,341 (GRCm38)	missense	probably benign	0.00
R1893:Aspm	UTSW	1	139,479,867 (GRCm38)	missense	probably damaging	1.00
R1911:Aspm	UTSW	1	139,478,094 (GRCm38)	missense	probably benign	0.06
R2103:Aspm	UTSW	1	139,491,665 (GRCm38)	missense	probably damaging	0.99
R2128:Aspm	UTSW	1	139,457,635 (GRCm38)	missense	probably benign	0.14
R2129:Aspm	UTSW	1	139,457,635 (GRCm38)	missense	probably benign	0.14
R2239:Aspm	UTSW	1	139,456,846 (GRCm38)	missense	possibly damaging	0.67
R2352:Aspm	UTSW	1	139,457,562 (GRCm38)	missense	probably benign	0.02
R2353:Aspm	UTSW	1	139,477,697 (GRCm38)	missense	probably damaging	1.00
R2380:Aspm	UTSW	1	139,479,348 (GRCm38)	missense	probably damaging	1.00
R2413:Aspm	UTSW	1	139,477,757 (GRCm38)	missense	probably damaging	1.00
R2421:Aspm	UTSW	1	139,488,487 (GRCm38)	missense	possibly damaging	0.49
R3607:Aspm	UTSW	1	139,480,668 (GRCm38)	missense	probably benign	0.13
R3711:Aspm	UTSW	1	139,458,100 (GRCm38)	missense	probably benign	0.17
R3718:Aspm	UTSW	1	139,490,427 (GRCm38)	missense	probably benign	0.31
R3718:Aspm	UTSW	1	139,480,889 (GRCm38)	missense	probably benign	0.09
R3741:Aspm	UTSW	1	139,478,619 (GRCm38)	missense	possibly damaging	0.47
R3788:Aspm	UTSW	1	139,463,203 (GRCm38)	missense	probably damaging	1.00
R3838:Aspm	UTSW	1	139,478,054 (GRCm38)	missense	probably benign	0.24
R3839:Aspm	UTSW	1	139,478,054 (GRCm38)	missense	probably benign	0.24
R3849:Aspm	UTSW	1	139,458,286 (GRCm38)	missense	probably benign	0.21
R4075:Aspm	UTSW	1	139,474,285 (GRCm38)	missense	probably damaging	1.00
R4080:Aspm	UTSW	1	139,470,755 (GRCm38)	missense	probably damaging	1.00
R4463:Aspm	UTSW	1	139,455,010 (GRCm38)	missense	possibly damaging	0.95
R4537:Aspm	UTSW	1	139,474,303 (GRCm38)	missense	probably benign	0.01
R4547:Aspm	UTSW	1	139,478,187 (GRCm38)	missense	possibly damaging	0.75
R4573:Aspm	UTSW	1	139,479,507 (GRCm38)	missense	probably damaging	0.98
R4680:Aspm	UTSW	1	139,480,671 (GRCm38)	missense	probably benign	0.05
R4807:Aspm	UTSW	1	139,477,919 (GRCm38)	missense	probably damaging	1.00
R4840:Aspm	UTSW	1	139,470,531 (GRCm38)	missense	possibly damaging	0.83
R4854:Aspm	UTSW	1	139,478,072 (GRCm38)	nonsense	probably null
R4859:Aspm	UTSW	1	139,469,393 (GRCm38)	missense	probably damaging	1.00
R4893:Aspm	UTSW	1	139,489,839 (GRCm38)	critical splice donor site	probably null
R4910:Aspm	UTSW	1	139,491,543 (GRCm38)	missense	probably damaging	1.00
R4953:Aspm	UTSW	1	139,471,734 (GRCm38)	missense	probably benign	0.00
R4974:Aspm	UTSW	1	139,478,010 (GRCm38)	missense	probably benign	0.03
R4981:Aspm	UTSW	1	139,470,760 (GRCm38)	splice site	probably null
R5082:Aspm	UTSW	1	139,478,676 (GRCm38)	nonsense	probably null
R5223:Aspm	UTSW	1	139,478,334 (GRCm38)	missense	probably damaging	1.00
R5268:Aspm	UTSW	1	139,464,295 (GRCm38)	missense	probably damaging	1.00
R5371:Aspm	UTSW	1	139,470,541 (GRCm38)	nonsense	probably null
R5377:Aspm	UTSW	1	139,470,395 (GRCm38)	splice site	probably null
R5377:Aspm	UTSW	1	139,457,483 (GRCm38)	missense	probably damaging	0.96
R5481:Aspm	UTSW	1	139,457,061 (GRCm38)	missense	possibly damaging	0.85
R5513:Aspm	UTSW	1	139,482,398 (GRCm38)	missense	probably damaging	1.00
R5578:Aspm	UTSW	1	139,470,717 (GRCm38)	missense	probably damaging	1.00
R5649:Aspm	UTSW	1	139,479,669 (GRCm38)	missense	probably benign
R5685:Aspm	UTSW	1	139,487,288 (GRCm38)	missense	probably benign	0.10
R5695:Aspm	UTSW	1	139,479,669 (GRCm38)	missense	probably benign
R5766:Aspm	UTSW	1	139,479,002 (GRCm38)	missense	probably damaging	0.99
R5964:Aspm	UTSW	1	139,455,227 (GRCm38)	intron	probably benign
R5993:Aspm	UTSW	1	139,479,531 (GRCm38)	missense	probably benign	0.28
R6027:Aspm	UTSW	1	139,463,056 (GRCm38)	missense	probably damaging	1.00
R6029:Aspm	UTSW	1	139,480,990 (GRCm38)	missense	possibly damaging	0.83
R6102:Aspm	UTSW	1	139,477,459 (GRCm38)	nonsense	probably null
R6188:Aspm	UTSW	1	139,479,239 (GRCm38)	missense	possibly damaging	0.79
R6257:Aspm	UTSW	1	139,482,053 (GRCm38)	splice site	probably null
R6433:Aspm	UTSW	1	139,473,683 (GRCm38)	missense	probably damaging	1.00
R6682:Aspm	UTSW	1	139,457,722 (GRCm38)	missense	possibly damaging	0.67
R6763:Aspm	UTSW	1	139,470,517 (GRCm38)	missense	possibly damaging	0.64
R6798:Aspm	UTSW	1	139,468,685 (GRCm38)	missense	possibly damaging	0.66
R6815:Aspm	UTSW	1	139,480,142 (GRCm38)	missense	probably benign	0.04
R6854:Aspm	UTSW	1	139,463,182 (GRCm38)	missense	possibly damaging	0.90
R6928:Aspm	UTSW	1	139,480,206 (GRCm38)	nonsense	probably null
R6943:Aspm	UTSW	1	139,480,542 (GRCm38)	missense	probably damaging	1.00
R6979:Aspm	UTSW	1	139,480,485 (GRCm38)	missense	probably damaging	1.00
R6998:Aspm	UTSW	1	139,469,472 (GRCm38)	missense	probably damaging	1.00
R7126:Aspm	UTSW	1	139,480,803 (GRCm38)	missense	probably benign	0.27
R7237:Aspm	UTSW	1	139,477,929 (GRCm38)	missense	possibly damaging	0.81
R7240:Aspm	UTSW	1	139,478,651 (GRCm38)	nonsense	probably null
R7272:Aspm	UTSW	1	139,458,328 (GRCm38)	missense	probably benign	0.14
R7427:Aspm	UTSW	1	139,457,616 (GRCm38)	missense	probably benign	0.01
R7519:Aspm	UTSW	1	139,490,336 (GRCm38)	missense	possibly damaging	0.53
R7776:Aspm	UTSW	1	139,479,846 (GRCm38)	missense	possibly damaging	0.85
R7875:Aspm	UTSW	1	139,455,134 (GRCm38)	missense	probably benign	0.02
R7883:Aspm	UTSW	1	139,478,667 (GRCm38)	missense	possibly damaging	0.47
R7964:Aspm	UTSW	1	139,480,686 (GRCm38)	missense	probably damaging	1.00
R8012:Aspm	UTSW	1	139,457,464 (GRCm38)	missense	probably benign	0.03
R8029:Aspm	UTSW	1	139,471,632 (GRCm38)	missense	probably benign	0.00
R8233:Aspm	UTSW	1	139,457,304 (GRCm38)	missense	probably benign	0.28
R8277:Aspm	UTSW	1	139,455,010 (GRCm38)	missense	probably damaging	1.00
R8345:Aspm	UTSW	1	139,464,273 (GRCm38)	nonsense	probably null
R8491:Aspm	UTSW	1	139,457,695 (GRCm38)	missense	probably damaging	0.98
R8511:Aspm	UTSW	1	139,457,308 (GRCm38)	missense	probably damaging	1.00
R8557:Aspm	UTSW	1	139,456,756 (GRCm38)	missense	probably benign	0.01
R8927:Aspm	UTSW	1	139,490,387 (GRCm38)	nonsense	probably null
R8928:Aspm	UTSW	1	139,490,387 (GRCm38)	nonsense	probably null
R8950:Aspm	UTSW	1	139,478,952 (GRCm38)	missense	probably damaging	1.00
R9033:Aspm	UTSW	1	139,478,127 (GRCm38)	missense	probably damaging	1.00
R9083:Aspm	UTSW	1	139,493,698 (GRCm38)	missense	possibly damaging	0.70
R9133:Aspm	UTSW	1	139,491,528 (GRCm38)	missense	probably damaging	1.00
R9160:Aspm	UTSW	1	139,490,124 (GRCm38)	missense	probably damaging	1.00
R9179:Aspm	UTSW	1	139,476,715 (GRCm38)	missense	probably damaging	1.00
R9265:Aspm	UTSW	1	139,461,444 (GRCm38)	missense	probably benign	0.24
R9400:Aspm	UTSW	1	139,479,903 (GRCm38)	missense	probably damaging	1.00
R9419:Aspm	UTSW	1	139,457,185 (GRCm38)	missense	probably benign	0.29
R9454:Aspm	UTSW	1	139,480,994 (GRCm38)	missense	probably benign	0.00
R9517:Aspm	UTSW	1	139,479,429 (GRCm38)	missense	probably damaging	1.00
R9524:Aspm	UTSW	1	139,480,869 (GRCm38)	missense	probably damaging	1.00
R9544:Aspm	UTSW	1	139,457,785 (GRCm38)	missense	probably benign	0.01
R9640:Aspm	UTSW	1	139,480,272 (GRCm38)	missense	possibly damaging	0.88
R9698:Aspm	UTSW	1	139,461,908 (GRCm38)	missense	probably benign	0.28
R9790:Aspm	UTSW	1	139,480,637 (GRCm38)	missense	probably damaging	0.98
R9791:Aspm	UTSW	1	139,480,637 (GRCm38)	missense	probably damaging	0.98
R9794:Aspm	UTSW	1	139,478,742 (GRCm38)	missense	probably damaging	0.99
X0063:Aspm	UTSW	1	139,458,090 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CCGAGCCCCAGCCATTACATTTTAC -3'
(R):5'- GGGAAGAGCATTCACTCATAGCCAG -3'

Sequencing Primer
(F):5'- AGCCATTACATTTTACAATCCACTC -3'
(R):5'- GTGATGTGATATATGTCCCCCA -3'

Posted On

2013-09-30