Mutagenetix > Incidental Mutation

Incidental Mutation 'R9274:Stk25'

703131

Institutional Source

Beutler Lab

Gene Symbol

Stk25

Ensembl Gene

ENSMUSG00000026277

Gene Name

serine/threonine kinase 25 (yeast)

Synonyms

SOK-1, Ste20-like, 1500019J11Rik, Ysk1

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9274 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

93548473-93581937 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 93552806 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 328 (S328G)

Ref Sequence

ENSEMBL: ENSMUSP00000027498 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027498] [ENSMUST00000120301] [ENSMUST00000133769] [ENSMUST00000186287]

AlphaFold

Q9Z2W1

Predicted Effect

probably benign
Transcript: ENSMUST00000027498
AA Change: S328G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000027498
Gene: ENSMUSG00000026277
AA Change: S328G

Domain	Start	End	E-Value	Type
S_TKc	20	270	2.92e-98	SMART
low complexity region	292	314	N/A	INTRINSIC
PDB:3W8H\|B	355	426	8e-43	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000120301

SMART Domains

Protein: ENSMUSP00000112725
Gene: ENSMUSG00000034066

Domain	Start	End	E-Value	Type
B41	40	234	1.48e-66	SMART
FERM_C	238	328	3.29e-35	SMART
FA	332	378	1.13e-15	SMART
low complexity region	409	421	N/A	INTRINSIC
low complexity region	440	459	N/A	INTRINSIC
low complexity region	462	473	N/A	INTRINSIC
low complexity region	478	492	N/A	INTRINSIC
RhoGEF	542	728	1.57e-56	SMART
PH	759	857	1.45e-19	SMART
low complexity region	879	890	N/A	INTRINSIC
PH	931	1029	2.62e-17	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000133769

SMART Domains

Protein: ENSMUSP00000117765
Gene: ENSMUSG00000026277

Domain	Start	End	E-Value	Type
Pfam:Pkinase	20	233	1.7e-63	PFAM
Pfam:Pkinase_Tyr	20	233	2.1e-47	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000186287

SMART Domains

Protein: ENSMUSP00000140396
Gene: ENSMUSG00000026277

Domain	Start	End	E-Value	Type
STYKc	20	107	1.9e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (65/65)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The encoded enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18. Multiple alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012]
PHENOTYPE: Germ line null mutants display normal cortical layers and neuronal migration. Acute loss of expression results in impaired neuronal migration. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actc1	A	T	2: 113,879,752 (GRCm39)	S236T	probably benign	Het
Adam30	A	G	3: 98,069,267 (GRCm39)	T239A	probably benign	Het
Aoc1l2	T	C	6: 48,907,341 (GRCm39)	F114L	possibly damaging	Het
Ascl2	G	A	7: 142,521,753 (GRCm39)	R232C	probably damaging	Het
Atp10a	C	A	7: 58,478,369 (GRCm39)	Q1471K	probably benign	Het
Avl9	T	A	6: 56,720,346 (GRCm39)	D461E	probably damaging	Het
Baiap3	T	A	17: 25,463,354 (GRCm39)	Y983F	probably damaging	Het
C1rl	A	G	6: 124,485,483 (GRCm39)	S285G	probably benign	Het
Cacna1i	G	A	15: 80,254,354 (GRCm39)	V772M	probably damaging	Het
Ccser2	T	A	14: 36,660,737 (GRCm39)	D472V	possibly damaging	Het
Cd244a	T	G	1: 171,401,928 (GRCm39)	V218G	probably benign	Het
Cdhr1	T	C	14: 36,802,564 (GRCm39)	K633R	probably benign	Het
Col14a1	A	G	15: 55,381,671 (GRCm39)	Q1748R	probably damaging	Het
Col6a3	T	A	1: 90,707,020 (GRCm39)	Y2638F	unknown	Het
Crnkl1	A	T	2: 145,765,836 (GRCm39)	F411L	probably damaging	Het
Crp	T	A	1: 172,526,072 (GRCm39)	F52L	possibly damaging	Het
Dnah12	A	G	14: 26,537,374 (GRCm39)	N2208D	probably benign	Het
Exd2	T	C	12: 80,539,674 (GRCm39)		probably null	Het
F13a1	A	T	13: 37,052,761 (GRCm39)	I726N	probably damaging	Het
Fmo6	G	A	1: 162,747,921 (GRCm39)	T381I	probably benign	Het
Gdpd5	T	C	7: 99,107,989 (GRCm39)	L522P	probably damaging	Het
Gm12790	T	A	4: 101,824,743 (GRCm39)	D175V	possibly damaging	Het
Gm3543	T	C	14: 41,802,125 (GRCm39)	R120G	probably damaging	Het
Gpatch2	T	A	1: 186,963,029 (GRCm39)	D273E	probably damaging	Het
Hlcs	A	G	16: 94,088,785 (GRCm39)	S66P	possibly damaging	Het
Hmcn1	T	C	1: 150,506,046 (GRCm39)	T3827A	probably benign	Het
Ism2	G	A	12: 87,331,827 (GRCm39)	Q237*	probably null	Het
Jph2	G	A	2: 163,239,547 (GRCm39)		probably benign	Het
Kcnb2	A	G	1: 15,781,723 (GRCm39)	Q865R	probably benign	Het
Kcnh6	C	G	11: 105,924,860 (GRCm39)	H941Q	possibly damaging	Het
Kcnk9	A	G	15: 72,384,224 (GRCm39)	V318A	unknown	Het
Lemd3	G	A	10: 120,814,717 (GRCm39)	A172V	possibly damaging	Het
Lifr	G	T	15: 7,217,591 (GRCm39)	A840S	probably damaging	Het
Lnpep	T	C	17: 17,758,837 (GRCm39)	T836A	probably benign	Het
Mapk13	A	G	17: 28,988,490 (GRCm39)	Y36C	probably damaging	Het
Mdm2	G	A	10: 117,541,081 (GRCm39)		probably benign	Het
Msmb	T	C	14: 31,870,130 (GRCm39)	M34T	probably benign	Het
Myo5a	A	G	9: 75,097,279 (GRCm39)	N1319S	possibly damaging	Het
Myot	T	A	18: 44,479,265 (GRCm39)	V334E	probably damaging	Het
Nalcn	A	G	14: 123,753,068 (GRCm39)	I306T	probably damaging	Het
Nicn1	C	T	9: 108,171,708 (GRCm39)	R163C	possibly damaging	Het
Nphp4	T	C	4: 152,640,056 (GRCm39)	Y981H	probably benign	Het
Nphs2	G	A	1: 156,144,416 (GRCm39)	R140Q	probably damaging	Het
Nsa2	C	T	13: 97,272,170 (GRCm39)	R17H	probably benign	Het
Nup98	C	A	7: 101,788,037 (GRCm39)	R1011L	probably benign	Het
Obox6	T	C	7: 15,567,766 (GRCm39)	H227R	possibly damaging	Het
Or10ak14	G	A	4: 118,610,883 (GRCm39)	A286V	probably benign	Het
Or1j14	T	C	2: 36,417,559 (GRCm39)	I45T	probably damaging	Het
Or4c1	A	C	2: 89,133,513 (GRCm39)	L141R	probably damaging	Het
P2rx2	C	T	5: 110,489,709 (GRCm39)	G202D	probably damaging	Het
Pcdh12	A	G	18: 38,415,950 (GRCm39)	W392R	probably damaging	Het
Plxna2	G	A	1: 194,471,136 (GRCm39)	G969D	probably damaging	Het
Prg4	T	C	1: 150,331,924 (GRCm39)	T250A	possibly damaging	Het
Rad54l	G	T	4: 115,967,667 (GRCm39)	P205Q	probably damaging	Het
Ralgapb	A	G	2: 158,278,539 (GRCm39)	I334M	probably damaging	Het
Ric3	C	T	7: 108,647,212 (GRCm39)	D204N	probably damaging	Het
Rnf10	A	T	5: 115,385,322 (GRCm39)	Y557*	probably null	Het
Ruvbl1	A	G	6: 88,474,334 (GRCm39)	I446V	probably benign	Het
Serinc3	A	T	2: 163,468,371 (GRCm39)	V361D	probably damaging	Het
Slc4a1	C	A	11: 102,242,047 (GRCm39)	V864L	probably benign	Het
Slf1	C	A	13: 77,191,669 (GRCm39)	*1055L	probably null	Het
Stpg1	T	C	4: 135,252,782 (GRCm39)	F178L	possibly damaging	Het
Tmem25	C	T	9: 44,706,336 (GRCm39)	R345H	probably benign	Het
Tssk1	A	T	16: 17,712,724 (GRCm39)	I170L	probably benign	Het
Tubg1	A	G	11: 101,017,241 (GRCm39)		probably benign	Het
Uchl3	A	G	14: 101,903,240 (GRCm39)	D33G	probably damaging	Het
Vmn1r152	A	T	7: 22,223,056 (GRCm39)	H222L	probably benign	Het
Zfp975	C	T	7: 42,312,299 (GRCm39)	V105I	probably benign	Het

Other mutations in Stk25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01291:Stk25	APN	1	93,551,145 (GRCm39)	splice site	probably null
IGL02952:Stk25	APN	1	93,553,798 (GRCm39)	missense	probably damaging	1.00
IGL03144:Stk25	APN	1	93,556,858 (GRCm39)	missense	probably damaging	1.00
ANU05:Stk25	UTSW	1	93,551,145 (GRCm39)	splice site	probably null
IGL02980:Stk25	UTSW	1	93,555,390 (GRCm39)	missense	probably damaging	1.00
PIT4531001:Stk25	UTSW	1	93,552,346 (GRCm39)	missense	probably benign
R0240:Stk25	UTSW	1	93,554,782 (GRCm39)	missense	probably damaging	1.00
R0240:Stk25	UTSW	1	93,554,782 (GRCm39)	missense	probably damaging	1.00
R0555:Stk25	UTSW	1	93,552,313 (GRCm39)	missense	probably benign	0.00
R1178:Stk25	UTSW	1	93,551,111 (GRCm39)	unclassified	probably benign
R1406:Stk25	UTSW	1	93,552,875 (GRCm39)	unclassified	probably benign
R1493:Stk25	UTSW	1	93,553,322 (GRCm39)	missense	probably benign	0.04
R2875:Stk25	UTSW	1	93,556,973 (GRCm39)	missense	possibly damaging	0.91
R4657:Stk25	UTSW	1	93,553,378 (GRCm39)	unclassified	probably benign
R4668:Stk25	UTSW	1	93,553,205 (GRCm39)	missense	probably damaging	1.00
R4686:Stk25	UTSW	1	93,551,142 (GRCm39)	splice site	probably null
R5089:Stk25	UTSW	1	93,552,330 (GRCm39)	missense	probably benign	0.05
R5493:Stk25	UTSW	1	93,563,031 (GRCm39)	missense	probably benign
R6013:Stk25	UTSW	1	93,553,181 (GRCm39)	critical splice donor site	probably null
R8723:Stk25	UTSW	1	93,553,666 (GRCm39)	missense	probably damaging	1.00
R8905:Stk25	UTSW	1	93,556,929 (GRCm39)	missense	probably damaging	1.00
R9249:Stk25	UTSW	1	93,552,806 (GRCm39)	missense	probably benign
X0063:Stk25	UTSW	1	93,553,683 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGGTCACAGGCACTTTGGAG -3'
(R):5'- GACTATCACCTAAATGCTGCAGCC -3'

Sequencing Primer
(F):5'- CTTTGGAGCTGACCTCATCAAAG -3'
(R):5'- TAAATGCTGCAGCCTGGAC -3'

Posted On

2022-03-25