Mutagenetix > Incidental Mutation

Incidental Mutation 'R9275:Vmn2r20'

703215

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r20

Ensembl Gene

ENSMUSG00000094145

Gene Name

vomeronasal 2, receptor 20

Synonyms

EG667180

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.598) question?

Stock #

R9275 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

123362221-123395020 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 123362394 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 797 (W797R)

Ref Sequence

ENSEMBL: ENSMUSP00000129145 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000172199]

AlphaFold

L7N2B1

Predicted Effect

probably damaging
Transcript: ENSMUST00000172199
AA Change: W797R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000129145
Gene: ENSMUSG00000094145
AA Change: W797R

Domain	Start	End	E-Value	Type
signal peptide	1	17	N/A	INTRINSIC
Pfam:ANF_receptor	81	473	5e-33	PFAM
Pfam:NCD3G	517	570	9.6e-24	PFAM
Pfam:7tm_3	601	838	1.4e-54	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

100% (66/66)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abr	A	G	11: 76,355,108 (GRCm39)	Y249H	probably damaging	Het
Afdn	T	A	17: 14,024,270 (GRCm39)	C59S	probably damaging	Het
Akap11	C	A	14: 78,751,149 (GRCm39)	V413F		Het
Ank3	T	C	10: 69,822,662 (GRCm39)	S444P	probably damaging	Het
Ankrd12	C	T	17: 66,344,599 (GRCm39)	E91K	possibly damaging	Het
Arhgap10	A	G	8: 78,137,665 (GRCm39)	S309P	probably damaging	Het
Arpc2	C	A	1: 74,276,041 (GRCm39)	F19L	probably benign	Het
Atp5f1a	C	T	18: 77,868,997 (GRCm39)	T457I	probably damaging	Het
Bsn	C	T	9: 107,988,819 (GRCm39)	R2311H	probably damaging	Het
C1ra	A	G	6: 124,494,383 (GRCm39)	T277A	probably benign	Het
Celsr3	C	G	9: 108,715,689 (GRCm39)	L2124V	probably benign	Het
Cenpv	T	C	11: 62,415,989 (GRCm39)	*253W	probably null	Het
Cfap54	A	T	10: 92,875,048 (GRCm39)	V479E	possibly damaging	Het
Clec1a	T	C	6: 129,428,564 (GRCm39)		probably benign	Het
Cmpk2	A	T	12: 26,519,568 (GRCm39)	Y73F	probably benign	Het
Cpne2	T	C	8: 95,281,643 (GRCm39)	I226T	possibly damaging	Het
Cyp2c67	C	T	19: 39,597,699 (GRCm39)	R433Q	probably damaging	Het
Dagla	A	G	19: 10,232,220 (GRCm39)	Y489H	probably damaging	Het
Dennd4a	C	A	9: 64,749,906 (GRCm39)	P166T	probably damaging	Het
Dnm2	C	T	9: 21,416,977 (GRCm39)	R837W	possibly damaging	Het
Enpp2	C	T	15: 54,713,484 (GRCm39)	R658Q	probably benign	Het
Gm10521	G	A	1: 171,724,030 (GRCm39)	V114I	unknown	Het
H2-T24	T	A	17: 36,328,276 (GRCm39)	E69V	probably damaging	Het
H6pd	T	A	4: 150,080,307 (GRCm39)	K179N	probably damaging	Het
Hsf2bp	C	T	17: 32,206,336 (GRCm39)	W265*	probably null	Het
Igfn1	C	A	1: 135,901,185 (GRCm39)	R431L	probably damaging	Het
Kcng2	T	C	18: 80,339,074 (GRCm39)	S405G	possibly damaging	Het
Leng9	T	C	7: 4,151,447 (GRCm39)	T410A	probably benign	Het
Lrp1b	T	C	2: 40,487,076 (GRCm39)	Y4557C		Het
Ltbp2	G	A	12: 84,837,864 (GRCm39)	P1192L	probably benign	Het
Mlkl	A	G	8: 112,043,055 (GRCm39)	V364A	probably benign	Het
Mns1	T	A	9: 72,356,507 (GRCm39)	F254Y	probably benign	Het
Naip1	T	A	13: 100,562,684 (GRCm39)	N827I	probably damaging	Het
Neb	C	T	2: 52,146,190 (GRCm39)	R2929H	probably damaging	Het
Or5m13	A	G	2: 85,749,046 (GRCm39)	Y259C	probably benign	Het
Or5v1b	T	A	17: 37,841,819 (GRCm39)	M317K	probably benign	Het
Pde10a	A	G	17: 9,200,488 (GRCm39)	*797W	probably null	Het
Pirb	T	G	7: 3,719,859 (GRCm39)	H429P	probably benign	Het
Plbd1	A	G	6: 136,594,286 (GRCm39)	L321P	probably damaging	Het
Pmfbp1	A	G	8: 110,262,471 (GRCm39)	I722V	probably benign	Het
Polr2b	A	G	5: 77,471,485 (GRCm39)	R274G	probably damaging	Het
Prune2	C	T	19: 17,101,144 (GRCm39)	T2216I	probably benign	Het
Psma3	A	T	12: 71,041,156 (GRCm39)	D252V	probably benign	Het
Ptpn1	C	T	2: 167,816,176 (GRCm39)	T230I	probably damaging	Het
Rhpn1	T	C	15: 75,585,120 (GRCm39)	V519A	possibly damaging	Het
Rnf213	T	C	11: 119,326,768 (GRCm39)	V1586A		Het
Rrp36	T	A	17: 46,983,306 (GRCm39)	K103*	probably null	Het
Ryr2	T	C	13: 11,897,976 (GRCm39)	T140A	probably benign	Het
Scn1a	T	A	2: 66,130,026 (GRCm39)	Y243F	probably damaging	Het
Sdk1	A	G	5: 141,941,953 (GRCm39)	T534A	possibly damaging	Het
Slc27a5	T	C	7: 12,731,640 (GRCm39)	D117G	probably damaging	Het
Slc2a1	A	T	4: 118,990,607 (GRCm39)	E246D	probably benign	Het
Smpd2	T	C	10: 41,363,685 (GRCm39)	D289G	probably benign	Het
Spag6	A	C	2: 18,703,985 (GRCm39)	E11A	probably benign	Het
Stard3	G	A	11: 98,262,931 (GRCm39)		probably benign	Het
Tcf19	A	G	17: 35,825,899 (GRCm39)	V86A	probably damaging	Het
Tcstv7a	T	C	13: 120,289,993 (GRCm39)	S68G	possibly damaging	Het
Tigit	A	T	16: 43,479,833 (GRCm39)	M154K	probably benign	Het
Trim34a	T	A	7: 103,910,201 (GRCm39)	N334K	probably damaging	Het
Tsen15	T	C	1: 152,259,098 (GRCm39)	I87V	probably damaging	Het
Ttn	A	T	2: 76,738,218 (GRCm39)	M4153K	unknown	Het
Uchl3	A	T	14: 101,905,963 (GRCm39)		probably null	Het
Usp1	A	G	4: 98,819,578 (GRCm39)	K347E	probably damaging	Het
Vmn1r73	T	C	7: 11,490,479 (GRCm39)	M99T	probably benign	Het
Vwa7	A	G	17: 35,238,712 (GRCm39)	T267A	probably damaging	Het
Zpld2	A	G	4: 133,922,770 (GRCm39)	L521P	probably damaging	Het

Other mutations in Vmn2r20

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01915:Vmn2r20	APN	6	123,370,924 (GRCm39)	missense	possibly damaging	0.89
IGL02653:Vmn2r20	APN	6	123,362,324 (GRCm39)	missense	probably damaging	1.00
IGL02686:Vmn2r20	APN	6	123,362,585 (GRCm39)	missense	probably benign	0.00
R0309:Vmn2r20	UTSW	6	123,363,063 (GRCm39)	missense	probably benign	0.18
R3724:Vmn2r20	UTSW	6	123,362,706 (GRCm39)	missense	probably benign	0.00
R6502:Vmn2r20	UTSW	6	123,373,342 (GRCm39)	missense	possibly damaging	0.51
R7039:Vmn2r20	UTSW	6	123,363,082 (GRCm39)	missense	probably damaging	0.98
R7218:Vmn2r20	UTSW	6	123,363,074 (GRCm39)	missense	probably damaging	1.00
R7372:Vmn2r20	UTSW	6	123,362,468 (GRCm39)	missense	probably damaging	1.00
R7509:Vmn2r20	UTSW	6	123,362,382 (GRCm39)	missense	probably benign	0.00
R7832:Vmn2r20	UTSW	6	123,362,882 (GRCm39)	missense	probably damaging	1.00
R8011:Vmn2r20	UTSW	6	123,373,369 (GRCm39)	missense	possibly damaging	0.71
R8118:Vmn2r20	UTSW	6	123,373,429 (GRCm39)	missense	probably damaging	1.00
R8839:Vmn2r20	UTSW	6	123,373,515 (GRCm39)	missense	possibly damaging	0.95
R9743:Vmn2r20	UTSW	6	123,373,369 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- AATGACAGCAGGGACACTTG -3'
(R):5'- CATCCTCTGGTGTAATGAGGG -3'

Sequencing Primer
(F):5'- GGACACTTGAATAAATAGCACTTGCC -3'
(R):5'- CCTCTGGTGTAATGAGGGATCCAC -3'

Posted On

2022-03-25