Mutagenetix > Incidental Mutation

Incidental Mutation 'R9280:Zfp87'

703582

Institutional Source

Beutler Lab

Gene Symbol

Zfp87

Ensembl Gene

ENSMUSG00000097333

Gene Name

zinc finger protein 87

Synonyms

Mzf22, 2210039O17Rik, 4631412O18Rik, Zfp72, KRAB4

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.116) question?

Stock #

R9280 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67663900-67674296 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 74520803 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 92 (T92A)

Ref Sequence

ENSEMBL: ENSMUSP00000089063 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000091481] [ENSMUST00000223163]

AlphaFold

Q8K2A4

Predicted Effect

probably benign
Transcript: ENSMUST00000091481
AA Change: T92A

PolyPhen 2 Score 0.158 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000089063
Gene: ENSMUSG00000069184
AA Change: T92A

Domain	Start	End	E-Value	Type
KRAB	1	32	3.57e-1	SMART
ZnF_C2H2	48	70	5.42e-2	SMART
ZnF_C2H2	76	98	1.18e-2	SMART
ZnF_C2H2	104	126	2.05e-2	SMART
ZnF_C2H2	132	154	2.3e-5	SMART
ZnF_C2H2	160	181	2.45e0	SMART
ZnF_C2H2	187	209	1.92e-2	SMART
ZnF_C2H2	215	237	5.59e-4	SMART
ZnF_C2H2	243	265	4.87e-4	SMART
ZnF_C2H2	271	293	4.94e-5	SMART
ZnF_C2H2	299	321	9.08e-4	SMART
ZnF_C2H2	327	349	1.13e-4	SMART
ZnF_C2H2	355	377	3.16e-3	SMART
ZnF_C2H2	383	405	2.24e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000223163
AA Change: T92A

PolyPhen 2 Score 0.158 (Sensitivity: 0.92; Specificity: 0.87)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acrbp	G	T	6: 125,039,938 (GRCm39)	V514L	probably damaging	Het
Actl11	A	G	9: 107,808,573 (GRCm39)	I965M	probably damaging	Het
Ank3	A	T	10: 69,818,021 (GRCm39)	S1468C	possibly damaging	Het
Aoc1l1	A	G	6: 48,955,116 (GRCm39)	D652G	possibly damaging	Het
Apobec3	A	C	15: 79,791,082 (GRCm39)	N392T		Het
Ccdc33	A	T	9: 57,965,549 (GRCm39)	M591K	probably benign	Het
Ccdc39	T	C	3: 33,870,153 (GRCm39)	E689G	probably damaging	Het
Cnot1	A	T	8: 96,497,227 (GRCm39)	M250K	probably benign	Het
Crppa	T	A	12: 36,571,975 (GRCm39)	S344R	probably benign	Het
Ctbs	A	T	3: 146,160,142 (GRCm39)	Y57F	probably damaging	Het
Dchs2	A	G	3: 83,189,255 (GRCm39)	I1540V	possibly damaging	Het
Dnah8	A	T	17: 31,004,071 (GRCm39)	Q3651H	possibly damaging	Het
Eif2a	A	G	3: 58,447,009 (GRCm39)		probably benign	Het
Entpd2	A	G	2: 25,289,511 (GRCm39)	N331S	possibly damaging	Het
F830045P16Rik	G	A	2: 129,314,774 (GRCm39)	P168S	probably damaging	Het
Fam76b	A	T	9: 13,751,012 (GRCm39)	H302L	possibly damaging	Het
Fancm	A	G	12: 65,153,612 (GRCm39)	E1356G	probably benign	Het
Fat2	G	A	11: 55,201,523 (GRCm39)	T517I	probably benign	Het
Glul	T	C	1: 153,783,611 (GRCm39)	C269R	probably benign	Het
Gm3404	C	T	5: 146,462,756 (GRCm39)	H40Y	possibly damaging	Het
Grb7	T	C	11: 98,345,248 (GRCm39)	F435S	probably damaging	Het
Ide	A	T	19: 37,295,490 (GRCm39)	Y191N		Het
Ide	T	C	19: 37,307,801 (GRCm39)		probably benign	Het
Ighv6-3	T	A	12: 114,355,420 (GRCm39)	T90S	probably damaging	Het
Il23r	A	G	6: 67,429,410 (GRCm39)	C311R	probably damaging	Het
Ilf2	A	G	3: 90,394,922 (GRCm39)	E390G	unknown	Het
Ints1	C	T	5: 139,750,469 (GRCm39)	C896Y	probably damaging	Het
Ipcef1	T	C	10: 6,850,736 (GRCm39)	E289G	probably benign	Het
Kbtbd2	G	A	6: 56,755,997 (GRCm39)	R580W	probably damaging	Het
Kif21b	T	C	1: 136,099,445 (GRCm39)		probably null	Het
Ltbp2	G	A	12: 84,837,864 (GRCm39)	P1192L	probably benign	Het
Mmp27	A	T	9: 7,579,812 (GRCm39)	I429L	probably benign	Het
Mmp8	A	G	9: 7,567,292 (GRCm39)	D431G	possibly damaging	Het
Mpeg1	A	G	19: 12,439,828 (GRCm39)	T429A	probably benign	Het
Mterf1a	A	T	5: 3,941,539 (GRCm39)	Y110N	probably damaging	Het
Myh4	G	A	11: 67,146,135 (GRCm39)	A1398T	probably damaging	Het
Nr3c2	T	A	8: 77,635,973 (GRCm39)	M358K	probably benign	Het
Nup50l	A	C	6: 96,141,982 (GRCm39)	L354R	probably damaging	Het
Or5d14	A	T	2: 87,880,458 (GRCm39)	F170Y	probably damaging	Het
Or5g25	G	A	2: 85,478,504 (GRCm39)	R54*	probably null	Het
Or7e173	A	C	9: 19,938,639 (GRCm39)	S198R	probably benign	Het
Otof	A	G	5: 30,528,894 (GRCm39)	F1838S	probably damaging	Het
Pappa2	G	A	1: 158,675,533 (GRCm39)	T1071I	possibly damaging	Het
Pcdhb15	A	G	18: 37,607,794 (GRCm39)	D342G	probably damaging	Het
Pcdhgb7	T	C	18: 37,886,585 (GRCm39)	L585P	probably damaging	Het
Pde1c	A	T	6: 56,114,505 (GRCm39)	S469R	probably benign	Het
Pla2g6	A	G	15: 79,197,314 (GRCm39)	F87L	probably benign	Het
Rag2	A	T	2: 101,460,145 (GRCm39)	M152L	probably benign	Het
Rasa1	C	T	13: 85,436,732 (GRCm39)	A99T	unknown	Het
Rasgrp1	T	C	2: 117,113,132 (GRCm39)	N760S	probably benign	Het
Rgsl1	A	T	1: 153,669,898 (GRCm39)	M232K	probably benign	Het
Ryr1	G	A	7: 28,802,389 (GRCm39)	H744Y	probably damaging	Het
Slc1a4	A	G	11: 20,282,325 (GRCm39)	S50P	probably damaging	Het
Slc25a36	A	C	9: 96,982,233 (GRCm39)	L34R	probably damaging	Het
Slc39a12	A	T	2: 14,401,003 (GRCm39)	I127F	probably benign	Het
Slco1a8	A	G	6: 141,939,978 (GRCm39)	L175P	possibly damaging	Het
Sntb1	T	C	15: 55,769,771 (GRCm39)	T73A	probably benign	Het
Tbc1d14	T	C	5: 36,680,268 (GRCm39)		probably benign	Het
Tll2	T	A	19: 41,077,309 (GRCm39)	E819V	possibly damaging	Het
Trappc13	C	T	13: 104,290,809 (GRCm39)	A181T	probably benign	Het
Trbj1-1	A	G	6: 41,510,830 (GRCm39)	R11G		Het
Ubash3b	A	C	9: 41,072,877 (GRCm39)	I12S	unknown	Het
Usp37	T	C	1: 74,489,699 (GRCm39)	K828E	probably damaging	Het
Usp8	G	C	2: 126,561,944 (GRCm39)	S46T	unknown	Het
Zfp418	A	G	7: 7,184,408 (GRCm39)	T124A	possibly damaging	Het

Other mutations in Zfp87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02165:Zfp87	APN	13	67,665,989 (GRCm39)	missense	probably damaging	0.99
IGL02372:Zfp87	APN	13	67,668,739 (GRCm39)	splice site	probably benign
IGL03160:Zfp87	APN	13	67,669,392 (GRCm39)	missense	probably damaging	1.00
R0653:Zfp87	UTSW	13	74,520,190 (GRCm39)	missense	probably damaging	1.00
R0711:Zfp87	UTSW	13	74,524,544 (GRCm39)	splice site	probably benign
R1498:Zfp87	UTSW	13	74,520,736 (GRCm39)	missense	probably benign	0.01
R3801:Zfp87	UTSW	13	67,669,334 (GRCm39)	missense	probably damaging	1.00
R4032:Zfp87	UTSW	13	74,520,449 (GRCm39)	missense	possibly damaging	0.62
R4629:Zfp87	UTSW	13	74,520,512 (GRCm39)	missense	probably damaging	1.00
R4950:Zfp87	UTSW	13	67,666,018 (GRCm39)	missense	probably benign	0.20
R5604:Zfp87	UTSW	13	67,665,945 (GRCm39)	missense	probably damaging	1.00
R6111:Zfp87	UTSW	13	74,520,504 (GRCm39)	missense	probably benign	0.31
R6130:Zfp87	UTSW	13	74,520,460 (GRCm39)	missense	possibly damaging	0.75
R6277:Zfp87	UTSW	13	74,520,643 (GRCm39)	nonsense	probably null
R6392:Zfp87	UTSW	13	67,664,986 (GRCm39)	missense	probably benign	0.00
R6800:Zfp87	UTSW	13	74,520,080 (GRCm39)	missense	probably benign	0.00
R6909:Zfp87	UTSW	13	74,519,861 (GRCm39)	missense	possibly damaging	0.47
R7009:Zfp87	UTSW	13	67,665,173 (GRCm39)	missense	probably damaging	1.00
R7183:Zfp87	UTSW	13	67,665,593 (GRCm39)	missense	probably damaging	1.00
R7298:Zfp87	UTSW	13	74,520,513 (GRCm39)	missense	possibly damaging	0.93
R7330:Zfp87	UTSW	13	74,523,153 (GRCm39)	missense	probably damaging	0.99
R7341:Zfp87	UTSW	13	74,520,467 (GRCm39)	missense	possibly damaging	0.68
R7448:Zfp87	UTSW	13	67,665,163 (GRCm39)	missense	probably benign	0.01
R7597:Zfp87	UTSW	13	67,665,412 (GRCm39)	missense	probably benign	0.06
R8696:Zfp87	UTSW	13	74,520,599 (GRCm39)	missense	probably damaging	1.00
R9429:Zfp87	UTSW	13	74,520,703 (GRCm39)	missense	probably damaging	1.00
R9780:Zfp87	UTSW	13	67,665,241 (GRCm39)	missense	probably damaging	1.00
R9782:Zfp87	UTSW	13	74,520,932 (GRCm39)	missense	probably benign	0.00
RF014:Zfp87	UTSW	13	74,523,173 (GRCm39)	missense	probably benign	0.17
Z1176:Zfp87	UTSW	13	67,674,275 (GRCm39)	start gained	probably benign
Z1177:Zfp87	UTSW	13	74,519,911 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CTGGGTAAGGCTTGAAGACCAG -3'
(R):5'- AATGCCATAGTGATTTGGGCTG -3'

Sequencing Primer
(F):5'- GTTAAAAGCTTTGCCACATTCTGGG -3'
(R):5'- ATAGTGATTTGGGCTGTAGAAATATG -3'

Posted On

2022-03-25