Mutagenetix > Incidental Mutation

Incidental Mutation 'R9286:Zfp955b'

703948

Institutional Source

Beutler Lab

Gene Symbol

Zfp955b

Ensembl Gene

ENSMUSG00000096910

Gene Name

zinc finger protein 955B

Synonyms

C430039G02Rik, A430003O12Rik, Gm4455

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R9286 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

33508518-33526215 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 33521683 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 384 (I384T)

Ref Sequence

ENSEMBL: ENSMUSP00000097011 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099414]

AlphaFold

L7N232

Predicted Effect

probably benign
Transcript: ENSMUST00000099414
AA Change: I384T

PolyPhen 2 Score 0.039 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000097011
Gene: ENSMUSG00000096910
AA Change: I384T

Domain	Start	End	E-Value	Type
KRAB	10	70	3.04e-14	SMART
ZnF_C2H2	230	252	7.68e0	SMART
ZnF_C2H2	258	280	5.72e-1	SMART
ZnF_C2H2	290	312	6.75e0	SMART
ZnF_C2H2	318	340	5.81e-2	SMART
ZnF_C2H2	346	368	3.16e-3	SMART
ZnF_C2H2	374	396	1.18e-2	SMART
ZnF_C2H2	402	424	7.78e-3	SMART
ZnF_C2H2	430	452	3.16e-3	SMART
ZnF_C2H2	458	480	1.1e-2	SMART
ZnF_C2H2	486	508	2.09e-3	SMART
ZnF_C2H2	514	536	6.67e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd2	G	C	15: 91,058,827 (GRCm39)	P539R	possibly damaging	Het
Adgrl3	A	T	5: 81,794,413 (GRCm39)	D478V	probably benign	Het
Adgrv1	A	T	13: 81,594,520 (GRCm39)	Y4165N	probably damaging	Het
Ago2	A	G	15: 72,997,065 (GRCm39)	L326P	probably damaging	Het
Akap6	A	C	12: 53,119,254 (GRCm39)	K1107T	possibly damaging	Het
Ank2	C	T	3: 126,846,381 (GRCm39)	A205T	probably damaging	Het
Ankrd27	T	C	7: 35,326,869 (GRCm39)	V738A	probably benign	Het
Asb1	A	G	1: 91,480,150 (GRCm39)	K291R	probably benign	Het
Cacna1e	G	T	1: 154,288,845 (GRCm39)	P1847T	probably damaging	Het
Cdh23	G	A	10: 60,143,306 (GRCm39)	A3005V	possibly damaging	Het
Cps1	T	G	1: 67,198,030 (GRCm39)	M365R	probably damaging	Het
Ctc1	T	G	11: 68,917,180 (GRCm39)		probably null	Het
Cyp2b10	G	T	7: 25,616,391 (GRCm39)	G333C	probably damaging	Het
Cyp2d12	A	G	15: 82,443,403 (GRCm39)	E455G	probably damaging	Het
Daam2	A	T	17: 49,786,922 (GRCm39)	D539E	possibly damaging	Het
Dip2a	T	A	10: 76,138,096 (GRCm39)	Y370F	probably benign	Het
Dok5	G	A	2: 170,672,099 (GRCm39)	E134K	possibly damaging	Het
Dync2h1	C	T	9: 6,941,668 (GRCm39)	A4164T	probably benign	Het
Ecm2	A	G	13: 49,683,696 (GRCm39)	Y558C		Het
Eif3b	A	G	5: 140,411,064 (GRCm39)	I172V	probably benign	Het
Flnb	T	C	14: 7,873,414 (GRCm38)	F237L	probably damaging	Het
Gdpd4	A	G	7: 97,647,639 (GRCm39)	I429V	probably damaging	Het
Grik1	T	C	16: 87,848,315 (GRCm39)	Y151C		Het
Gys2	C	A	6: 142,376,037 (GRCm39)	V542L	possibly damaging	Het
Hmx1	A	G	5: 35,546,776 (GRCm39)	T106A	probably benign	Het
Il1rap	T	C	16: 26,517,604 (GRCm39)	V268A	possibly damaging	Het
Kansl3	T	C	1: 36,387,720 (GRCm39)	T543A	probably benign	Het
Kcnk1	T	A	8: 126,752,148 (GRCm39)		probably null	Het
Larp7	CCTTCTT	CCTT	3: 127,340,008 (GRCm39)		probably benign	Het
Llgl2	T	C	11: 115,740,844 (GRCm39)	F449L	probably damaging	Het
Lmod2	A	G	6: 24,603,712 (GRCm39)	E229G	probably damaging	Het
Loxl4	A	T	19: 42,586,047 (GRCm39)	V699E	possibly damaging	Het
Miga2	A	G	2: 30,273,609 (GRCm39)	D489G	probably benign	Het
Nlrp1b	T	A	11: 71,060,573 (GRCm39)	H748L	probably benign	Het
Nudcd2	T	A	11: 40,627,403 (GRCm39)	Y108N	probably damaging	Het
Or10g1	G	A	14: 52,648,075 (GRCm39)	R85*	probably null	Het
Or52b2	A	C	7: 104,985,971 (GRCm39)	C317W	probably damaging	Het
Or5b120	T	A	19: 13,479,791 (GRCm39)	I28N	possibly damaging	Het
Pcdhb17	T	A	18: 37,619,422 (GRCm39)	V404E	probably benign	Het
Pde4dip	T	C	3: 97,607,183 (GRCm39)	D2084G	probably damaging	Het
Phf20	A	T	2: 156,134,470 (GRCm39)	K552M	probably damaging	Het
Plcg2	A	G	8: 118,331,976 (GRCm39)	D854G	probably benign	Het
Psen1	A	G	12: 83,775,549 (GRCm39)	N316D	probably benign	Het
Rabgap1l	G	A	1: 160,051,818 (GRCm39)	Q361*	probably null	Het
Ralb	A	T	1: 119,399,544 (GRCm39)	D171E	probably benign	Het
Rev3l	T	C	10: 39,682,947 (GRCm39)	I355T	possibly damaging	Het
Rttn	A	G	18: 88,995,849 (GRCm39)	K211E	probably benign	Het
Scg2	A	G	1: 79,413,653 (GRCm39)	S357P	probably damaging	Het
Sh2b1	TGGGGACCAGCTCAGCCACGGGGACCAGCTC	TGGGGACCAGCTCAGCCACGGGGACCAGCTCAGCCACGGGGACCAGCTC	7: 126,066,742 (GRCm39)		probably benign	Het
Slc17a5	A	T	9: 78,445,566 (GRCm39)	W456R	probably damaging	Het
Slf1	A	T	13: 77,191,932 (GRCm39)	D967E	probably damaging	Het
Spryd3	A	T	15: 102,041,869 (GRCm39)	V51E	possibly damaging	Het
Tgfbi	A	G	13: 56,773,563 (GRCm39)	H187R	probably damaging	Het
Tmc3	A	G	7: 83,252,643 (GRCm39)	E348G	probably damaging	Het
Tmem45a2	A	G	16: 56,867,332 (GRCm39)	I123T	probably damaging	Het
Tonsl	G	A	15: 76,515,213 (GRCm39)	H1058Y	probably damaging	Het
Trav13d-1	A	G	14: 53,089,050 (GRCm39)	K20E	probably benign	Het
Trpm2	C	A	10: 77,777,014 (GRCm39)	V428L	probably benign	Het
Usp25	A	G	16: 76,904,864 (GRCm39)	Y810C	probably damaging	Het
Vmn1r40	T	A	6: 89,692,079 (GRCm39)	F299I	probably benign	Het
Vmn2r18	A	G	5: 151,499,175 (GRCm39)	F430L	probably benign	Het
Vps13c	G	A	9: 67,880,203 (GRCm39)	V3462I	probably benign	Het
Zmym4	T	C	4: 126,783,812 (GRCm39)	D1138G	probably damaging	Het

Other mutations in Zfp955b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00537:Zfp955b	APN	17	33,521,847 (GRCm39)	missense	probably damaging	1.00
IGL02073:Zfp955b	APN	17	33,519,564 (GRCm39)	missense	possibly damaging	0.69
IGL02126:Zfp955b	APN	17	33,521,238 (GRCm39)	nonsense	probably null
IGL02237:Zfp955b	APN	17	33,520,893 (GRCm39)	missense	probably damaging	1.00
IGL02587:Zfp955b	APN	17	33,519,624 (GRCm39)	missense	probably damaging	1.00
IGL02971:Zfp955b	APN	17	33,519,940 (GRCm39)	missense	probably benign	0.11
IGL03034:Zfp955b	APN	17	33,521,142 (GRCm39)	missense	probably benign	0.22
IGL03493:Zfp955b	APN	17	33,521,519 (GRCm39)	missense	probably benign	0.35
R0269:Zfp955b	UTSW	17	33,524,437 (GRCm39)	missense	probably damaging	1.00
R0373:Zfp955b	UTSW	17	33,521,496 (GRCm39)	missense	probably benign
R0617:Zfp955b	UTSW	17	33,524,437 (GRCm39)	missense	probably damaging	1.00
R0684:Zfp955b	UTSW	17	33,521,947 (GRCm39)	missense	probably benign	0.00
R1778:Zfp955b	UTSW	17	33,521,788 (GRCm39)	missense	probably benign	0.07
R1874:Zfp955b	UTSW	17	33,524,427 (GRCm39)	missense	probably benign	0.10
R3893:Zfp955b	UTSW	17	33,521,968 (GRCm39)	missense	probably benign	0.01
R3938:Zfp955b	UTSW	17	33,524,390 (GRCm39)	missense	probably damaging	1.00
R4082:Zfp955b	UTSW	17	33,521,129 (GRCm39)	missense	probably benign	0.08
R4672:Zfp955b	UTSW	17	33,524,233 (GRCm39)	unclassified	probably benign
R4956:Zfp955b	UTSW	17	33,524,209 (GRCm39)	unclassified	probably benign
R4998:Zfp955b	UTSW	17	33,524,125 (GRCm39)	unclassified	probably benign
R5276:Zfp955b	UTSW	17	33,522,031 (GRCm39)	missense	probably damaging	1.00
R5341:Zfp955b	UTSW	17	33,524,095 (GRCm39)	unclassified	probably benign
R5558:Zfp955b	UTSW	17	33,521,161 (GRCm39)	missense	possibly damaging	0.88
R6086:Zfp955b	UTSW	17	33,521,478 (GRCm39)	missense	probably benign
R6170:Zfp955b	UTSW	17	33,521,084 (GRCm39)	missense	probably benign	0.00
R6306:Zfp955b	UTSW	17	33,522,160 (GRCm39)	missense	probably benign	0.07
R6519:Zfp955b	UTSW	17	33,521,051 (GRCm39)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- GGGAAAGCATGCATTCATTCTTATC -3'
(R):5'- TCATGGTTGTAACGGTTGCCC -3'

Sequencing Primer
(F):5'- TCAACTTCTTCAGCATGAAAGAAGC -3'
(R):5'- TTGCCCGCACTGCTAAAG -3'

Posted On

2022-03-25