Mutagenetix > Incidental Mutation

Incidental Mutation 'R9287:Tln2'

704024

Institutional Source

Beutler Lab

Gene Symbol

Tln2

Ensembl Gene

ENSMUSG00000052698

Gene Name

talin 2

Synonyms

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.185) question?

Stock #

R9287 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67124369-67466985 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 67277980 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Leucine at position 343 (V343L)

Ref Sequence

ENSEMBL: ENSMUSP00000035272 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039662] [ENSMUST00000040025] [ENSMUST00000215784]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000039662
AA Change: V343L

PolyPhen 2 Score 0.200 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000035272
Gene: ENSMUSG00000052698
AA Change: V343L

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	3.4e-59	PFAM
Pfam:I_LWEQ	661	765	1.9e-10	PFAM
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	2.6e-67	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2384	2531	2.5e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000040025
AA Change: V343L

PolyPhen 2 Score 0.200 (Sensitivity: 0.92; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000039633
Gene: ENSMUSG00000052698
AA Change: V343L

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	8.5e-78	PFAM
low complexity region	674	693	N/A	INTRINSIC
internal_repeat_2	703	763	2.05e-7	PROSPERO
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	9.9e-72	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2383	2533	4.3e-51	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215784
AA Change: V345L

PolyPhen 2 Score 0.135 (Sensitivity: 0.92; Specificity: 0.86)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (109/109)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal muscle morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 108 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9430097D07Rik	T	A	2: 32,465,178 (GRCm39)		probably benign	Het
Aadacl2	C	A	3: 59,932,573 (GRCm39)	H363N	probably damaging	Het
Ace3	T	G	11: 105,888,246 (GRCm39)	S319A	probably damaging	Het
Amer3	C	T	1: 34,627,900 (GRCm39)	P713L	possibly damaging	Het
Aoah	T	C	13: 21,186,879 (GRCm39)	L453P	probably damaging	Het
Asz1	A	T	6: 18,051,290 (GRCm39)	L463Q	possibly damaging	Het
Bpifb6	T	A	2: 153,746,535 (GRCm39)	V143D	probably damaging	Het
Cad	T	A	5: 31,230,000 (GRCm39)	M1499K	possibly damaging	Het
Casp9	T	A	4: 141,534,471 (GRCm39)	C294S	probably benign	Het
Ccdc7b	T	A	8: 129,890,321 (GRCm39)	S65T	probably benign	Het
Cdh23	G	A	10: 60,143,306 (GRCm39)	A3005V	possibly damaging	Het
Cfap54	T	C	10: 92,805,565 (GRCm39)	Y1515C	possibly damaging	Het
Chrm5	A	C	2: 112,309,610 (GRCm39)	F502C	probably damaging	Het
Cnst	A	T	1: 179,407,108 (GRCm39)	T52S	possibly damaging	Het
Cntn6	T	C	6: 104,809,471 (GRCm39)	I502T	possibly damaging	Het
Col7a1	G	A	9: 108,787,457 (GRCm39)	V617M	unknown	Het
Ctsb	A	G	14: 63,370,875 (GRCm39)	D29G	probably benign	Het
Cyp3a44	T	A	5: 145,725,202 (GRCm39)	Q333L	possibly damaging	Het
Dnajc27	T	C	12: 4,146,256 (GRCm39)	V95A	possibly damaging	Het
Eea1	T	A	10: 95,831,445 (GRCm39)	Y179N	probably damaging	Het
Erbb2	T	C	11: 98,326,107 (GRCm39)	M961T	probably damaging	Het
Fat4	G	A	3: 38,945,781 (GRCm39)	G1558D	probably damaging	Het
Foxh1	T	C	15: 76,553,126 (GRCm39)	E196G	probably damaging	Het
Gcc2	T	A	10: 58,105,217 (GRCm39)	L151*	probably null	Het
Gcdh	C	T	8: 85,616,313 (GRCm39)	G294D	probably damaging	Het
Gcnt3	A	T	9: 69,941,693 (GRCm39)	F292I	probably damaging	Het
Glod4	T	C	11: 76,128,510 (GRCm39)	S131G	probably benign	Het
Gpc2	G	A	5: 138,272,586 (GRCm39)	L576F	unknown	Het
Gphn	G	A	12: 78,609,646 (GRCm39)	S330N	possibly damaging	Het
Heatr5a	C	T	12: 51,967,260 (GRCm39)	C872Y	probably damaging	Het
Hephl1	G	A	9: 14,995,775 (GRCm39)	S449L	probably benign	Het
Hrh2	G	T	13: 54,368,358 (GRCm39)	M111I	probably benign	Het
Igfn1	A	G	1: 135,925,544 (GRCm39)	V70A	probably benign	Het
Il2	G	A	3: 37,179,988 (GRCm39)	T23I	probably damaging	Het
Irak4	A	G	15: 94,460,917 (GRCm39)	T382A	possibly damaging	Het
Itih2	A	G	2: 10,128,297 (GRCm39)	S135P	possibly damaging	Het
Kansl3	C	T	1: 36,388,497 (GRCm39)	D457N	probably damaging	Het
Kcns3	A	G	12: 11,141,601 (GRCm39)	I366T	probably damaging	Het
Kif26a	C	A	12: 112,145,719 (GRCm39)	Y1743*	probably null	Het
Lama4	A	T	10: 38,981,960 (GRCm39)	I1730F	probably damaging	Het
Lax1	T	C	1: 133,607,931 (GRCm39)	N270S	probably benign	Het
Lrp1	G	T	10: 127,403,233 (GRCm39)	D2113E	probably damaging	Het
Lrp6	A	G	6: 134,483,259 (GRCm39)	V482A	probably benign	Het
Luzp2	A	G	7: 54,914,108 (GRCm39)		probably benign	Het
Mc5r	C	A	18: 68,472,200 (GRCm39)	D186E	probably damaging	Het
Mcph1	A	T	8: 18,657,293 (GRCm39)		probably null	Het
Mgam	A	G	6: 40,705,905 (GRCm39)		probably benign	Het
Mmrn1	A	C	6: 60,952,939 (GRCm39)	T407P	probably damaging	Het
Mrpl1	T	C	5: 96,386,806 (GRCm39)	V265A	probably benign	Het
Mrpl15	C	T	1: 4,846,856 (GRCm39)	G240D	probably damaging	Het
Msx1	A	T	5: 37,978,795 (GRCm39)	M240K	probably damaging	Het
Mtf1	T	C	4: 124,724,934 (GRCm39)	L337P	probably damaging	Het
Muc5ac	T	A	7: 141,361,626 (GRCm39)	C1646S	probably damaging	Het
Mvb12a	C	A	8: 71,999,638 (GRCm39)	T219N	probably damaging	Het
Myo16	G	A	8: 10,526,114 (GRCm39)	V885M	unknown	Het
N4bp2	T	A	5: 65,960,855 (GRCm39)	S509T	probably benign	Het
Nckap1	A	G	2: 80,383,750 (GRCm39)	V144A	possibly damaging	Het
Nkx2-6	G	T	14: 69,412,404 (GRCm39)	G191C	possibly damaging	Het
Nmnat2	T	C	1: 152,962,138 (GRCm39)	I126T	probably damaging	Het
Nrp2	C	T	1: 62,835,014 (GRCm39)	R863W	probably damaging	Het
Nup188	C	T	2: 30,226,726 (GRCm39)	R1168C	probably damaging	Het
Oas3	T	A	5: 120,892,754 (GRCm39)	D1091V	probably damaging	Het
Optn	T	A	2: 5,036,126 (GRCm39)	Q452L	probably damaging	Het
Or8b47	C	T	9: 38,435,082 (GRCm39)	T18I	probably damaging	Het
Pcca	G	A	14: 122,854,178 (GRCm39)	V157I	probably benign	Het
Pcdha9	A	G	18: 37,132,281 (GRCm39)	D450G	probably benign	Het
Pcnx1	T	A	12: 82,042,323 (GRCm39)	S38T	probably benign	Het
Phrf1	C	G	7: 140,840,055 (GRCm39)	D1083E	probably benign	Het
Plaat5	T	A	19: 7,596,691 (GRCm39)	Y159*	probably null	Het
Plcb4	G	A	2: 135,829,817 (GRCm39)	A947T	probably benign	Het
Ppp1r3g	G	A	13: 36,152,834 (GRCm39)	D85N	possibly damaging	Het
Pramel34	G	A	5: 93,785,969 (GRCm39)	H104Y	possibly damaging	Het
Prom2	A	C	2: 127,380,185 (GRCm39)	V349G	probably damaging	Het
Prrc2c	C	T	1: 162,541,843 (GRCm39)	S382N	probably benign	Het
Rai14	A	T	15: 10,592,204 (GRCm39)	N230K	probably benign	Het
Rexo5	A	T	7: 119,402,025 (GRCm39)	K142I	probably damaging	Het
Rgs22	T	C	15: 36,098,409 (GRCm39)	H484R	probably damaging	Het
Rims2	C	T	15: 39,543,086 (GRCm39)	A1440V	probably damaging	Het
Serpinb1a	T	C	13: 33,026,946 (GRCm39)	E332G	probably damaging	Het
Sh2b1	TGGGGACCAGCTCAGCCACGGGGACCAGCTC	TGGGGACCAGCTCAGCCACGGGGACCAGCTCAGCCACGGGGACCAGCTC	7: 126,066,742 (GRCm39)		probably benign	Het
Slc26a7	C	T	4: 14,516,165 (GRCm39)	G555S	possibly damaging	Het
Slc4a2	A	T	5: 24,639,123 (GRCm39)	D386V	probably damaging	Het
Slc6a17	C	T	3: 107,384,551 (GRCm39)	V350M	probably damaging	Het
Smc2	A	G	4: 52,449,361 (GRCm39)	Y174C	probably damaging	Het
Smoc2	A	G	17: 14,619,686 (GRCm39)	Y355C	probably damaging	Het
Smpd1	T	A	7: 105,204,442 (GRCm39)	V107E	probably benign	Het
Snapc1	T	C	12: 74,018,773 (GRCm39)		probably benign	Het
Spata31e2	T	C	1: 26,722,426 (GRCm39)	D918G	possibly damaging	Het
Steap4	T	G	5: 8,026,683 (GRCm39)	F215L	probably benign	Het
Tbc1d1	T	A	5: 64,435,364 (GRCm39)	S501T	probably damaging	Het
Tec	A	G	5: 72,926,117 (GRCm39)	Y312H	probably damaging	Het
Ticrr	C	A	7: 79,343,516 (GRCm39)	T1127K	possibly damaging	Het
Tlk2	A	G	11: 105,147,722 (GRCm39)	D438G	probably benign	Het
Tmem156	T	A	5: 65,231,148 (GRCm39)	I241F	probably damaging	Het
Tmtc1	G	T	6: 148,186,390 (GRCm39)	N559K	probably benign	Het
Trbv4	A	T	6: 41,036,696 (GRCm39)	I74F	probably benign	Het
Trmt1l	C	T	1: 151,328,899 (GRCm39)	P524S	probably damaging	Het
Trpa1	A	T	1: 14,956,040 (GRCm39)	C776*	probably null	Het
Ttc41	T	A	10: 86,599,830 (GRCm39)	S1043R	probably benign	Het
Ttn	A	G	2: 76,576,646 (GRCm39)	L24749S	probably damaging	Het
Ube2o	C	T	11: 116,471,942 (GRCm39)	G100R	probably damaging	Het
Unc5b	T	C	10: 60,609,532 (GRCm39)	E588G	possibly damaging	Het
Usp43	G	T	11: 67,770,922 (GRCm39)	Q571K	probably damaging	Het
Vmn1r127	G	A	7: 21,052,927 (GRCm39)	P287L	possibly damaging	Het
Wasf3	T	A	5: 146,397,857 (GRCm39)	M208K	possibly damaging	Het
Xab2	A	G	8: 3,663,000 (GRCm39)	F527S	possibly damaging	Het
Zfp644	T	G	5: 106,785,774 (GRCm39)	N258H	possibly damaging	Het
Zfp947	A	C	17: 22,364,594 (GRCm39)	F360C	probably damaging	Het

Other mutations in Tln2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00468:Tln2	APN	9	67,251,469 (GRCm39)	missense	possibly damaging	0.59
IGL01110:Tln2	APN	9	67,157,864 (GRCm39)	nonsense	probably null
IGL01112:Tln2	APN	9	67,219,093 (GRCm39)	missense	probably damaging	1.00
IGL01307:Tln2	APN	9	67,302,749 (GRCm39)	missense	probably benign	0.25
IGL01374:Tln2	APN	9	67,169,205 (GRCm39)	missense	probably damaging	1.00
IGL01625:Tln2	APN	9	67,277,905 (GRCm39)	missense	probably damaging	1.00
IGL01865:Tln2	APN	9	67,157,896 (GRCm39)	nonsense	probably null
IGL01999:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02002:Tln2	APN	9	67,263,980 (GRCm39)	missense	probably damaging	0.98
IGL02005:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02015:Tln2	APN	9	67,268,721 (GRCm39)	splice site	probably benign
IGL02368:Tln2	APN	9	67,148,092 (GRCm39)	splice site	probably benign
IGL02444:Tln2	APN	9	67,165,874 (GRCm39)	splice site	probably benign
IGL02646:Tln2	APN	9	67,163,278 (GRCm39)	missense	probably benign	0.43
IGL02744:Tln2	APN	9	67,136,658 (GRCm39)	nonsense	probably null
IGL02869:Tln2	APN	9	67,128,807 (GRCm39)	splice site	probably benign
IGL02930:Tln2	APN	9	67,300,944 (GRCm39)	nonsense	probably null
IGL03100:Tln2	APN	9	67,203,019 (GRCm39)	missense	probably damaging	1.00
IGL03326:Tln2	APN	9	67,241,539 (GRCm39)	missense	possibly damaging	0.67
Harrier	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
Marsh	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
BB008:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
BB018:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0107:Tln2	UTSW	9	67,277,988 (GRCm39)	missense	probably damaging	1.00
R0494:Tln2	UTSW	9	67,262,479 (GRCm39)	missense	probably benign	0.22
R0884:Tln2	UTSW	9	67,278,015 (GRCm39)	missense	probably damaging	1.00
R0947:Tln2	UTSW	9	67,203,095 (GRCm39)	missense	probably benign	0.08
R0989:Tln2	UTSW	9	67,136,736 (GRCm39)	missense	probably damaging	1.00
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1486:Tln2	UTSW	9	67,219,121 (GRCm39)	missense	probably damaging	1.00
R1527:Tln2	UTSW	9	67,179,950 (GRCm39)	missense	possibly damaging	0.95
R1584:Tln2	UTSW	9	67,203,696 (GRCm39)	missense	probably damaging	1.00
R1636:Tln2	UTSW	9	67,213,814 (GRCm39)	missense	probably damaging	1.00
R1656:Tln2	UTSW	9	67,134,389 (GRCm39)	missense	possibly damaging	0.81
R1707:Tln2	UTSW	9	67,283,089 (GRCm39)	missense	probably benign	0.00
R1749:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1751:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1761:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1767:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1815:Tln2	UTSW	9	67,136,705 (GRCm39)	missense	probably damaging	1.00
R1840:Tln2	UTSW	9	67,249,325 (GRCm39)	missense	probably damaging	1.00
R1847:Tln2	UTSW	9	67,269,969 (GRCm39)	nonsense	probably null
R1964:Tln2	UTSW	9	67,249,417 (GRCm39)	missense	probably benign	0.00
R1968:Tln2	UTSW	9	67,163,183 (GRCm39)	missense	probably damaging	1.00
R2036:Tln2	UTSW	9	67,179,986 (GRCm39)	missense	possibly damaging	0.76
R2038:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R2152:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2153:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2154:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2191:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2192:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2201:Tln2	UTSW	9	67,283,039 (GRCm39)	missense	probably damaging	1.00
R3116:Tln2	UTSW	9	67,262,421 (GRCm39)	missense	probably benign	0.10
R3151:Tln2	UTSW	9	67,237,829 (GRCm39)	critical splice donor site	probably null
R3795:Tln2	UTSW	9	67,163,197 (GRCm39)	missense	probably damaging	0.97
R3953:Tln2	UTSW	9	67,277,911 (GRCm39)	missense	probably damaging	1.00
R4450:Tln2	UTSW	9	67,251,347 (GRCm39)	critical splice donor site	probably null
R4685:Tln2	UTSW	9	67,209,854 (GRCm39)	missense	probably damaging	1.00
R4688:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R4696:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4697:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4700:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4701:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4741:Tln2	UTSW	9	67,293,837 (GRCm39)	critical splice donor site	probably null
R4806:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4807:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4808:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4967:Tln2	UTSW	9	67,262,407 (GRCm39)	missense	probably damaging	0.97
R5061:Tln2	UTSW	9	67,261,750 (GRCm39)	missense	probably benign
R5092:Tln2	UTSW	9	67,163,310 (GRCm39)	missense	probably benign	0.13
R5093:Tln2	UTSW	9	67,241,596 (GRCm39)	missense	probably benign	0.44
R5126:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R5204:Tln2	UTSW	9	67,261,764 (GRCm39)	missense	probably benign	0.00
R5236:Tln2	UTSW	9	67,273,205 (GRCm39)	missense	probably damaging	0.99
R5287:Tln2	UTSW	9	67,149,641 (GRCm39)	missense	probably damaging	1.00
R5568:Tln2	UTSW	9	67,219,147 (GRCm39)	missense	probably damaging	1.00
R5571:Tln2	UTSW	9	67,241,602 (GRCm39)	missense	possibly damaging	0.88
R5642:Tln2	UTSW	9	67,203,640 (GRCm39)	missense	probably benign	0.01
R5711:Tln2	UTSW	9	67,299,829 (GRCm39)	missense	probably benign	0.00
R5776:Tln2	UTSW	9	67,165,532 (GRCm39)	missense	probably damaging	1.00
R5791:Tln2	UTSW	9	67,293,887 (GRCm39)	missense	probably damaging	0.98
R5866:Tln2	UTSW	9	67,174,150 (GRCm39)	missense	probably damaging	1.00
R5888:Tln2	UTSW	9	67,136,685 (GRCm39)	missense	probably damaging	1.00
R5902:Tln2	UTSW	9	67,269,999 (GRCm39)	missense	probably benign	0.02
R6106:Tln2	UTSW	9	67,230,302 (GRCm39)	missense	probably damaging	0.99
R6175:Tln2	UTSW	9	67,131,363 (GRCm39)	missense	probably damaging	1.00
R6385:Tln2	UTSW	9	67,185,411 (GRCm39)	missense	probably benign	0.45
R6430:Tln2	UTSW	9	67,179,947 (GRCm39)	missense	probably damaging	1.00
R6441:Tln2	UTSW	9	67,179,971 (GRCm39)	missense	probably damaging	1.00
R6738:Tln2	UTSW	9	67,293,946 (GRCm39)	missense	possibly damaging	0.91
R6776:Tln2	UTSW	9	67,170,187 (GRCm39)	missense	probably damaging	1.00
R6794:Tln2	UTSW	9	67,193,840 (GRCm39)	missense	probably benign	0.07
R6850:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R6907:Tln2	UTSW	9	67,304,917 (GRCm39)	missense	probably damaging	0.98
R6909:Tln2	UTSW	9	67,299,814 (GRCm39)	missense	probably damaging	0.97
R6951:Tln2	UTSW	9	67,165,767 (GRCm39)	missense	probably damaging	0.97
R7015:Tln2	UTSW	9	67,269,929 (GRCm39)	missense	possibly damaging	0.55
R7051:Tln2	UTSW	9	67,253,699 (GRCm39)	missense	probably benign	0.00
R7246:Tln2	UTSW	9	67,170,261 (GRCm39)	missense	probably damaging	1.00
R7292:Tln2	UTSW	9	67,253,743 (GRCm39)	missense	probably benign
R7753:Tln2	UTSW	9	67,302,755 (GRCm39)	missense	probably damaging	1.00
R7868:Tln2	UTSW	9	67,255,508 (GRCm39)	missense	probably damaging	1.00
R7931:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R8023:Tln2	UTSW	9	67,131,346 (GRCm39)	missense	probably damaging	1.00
R8081:Tln2	UTSW	9	67,264,029 (GRCm39)	missense	probably damaging	1.00
R8164:Tln2	UTSW	9	67,226,702 (GRCm39)	missense	probably benign	0.31
R8192:Tln2	UTSW	9	67,253,811 (GRCm39)	nonsense	probably null
R8495:Tln2	UTSW	9	67,261,749 (GRCm39)	missense	probably benign	0.01
R8734:Tln2	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
R8739:Tln2	UTSW	9	67,165,555 (GRCm39)	missense	probably damaging	1.00
R8757:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8759:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8770:Tln2	UTSW	9	67,230,304 (GRCm39)	missense	probably benign
R8781:Tln2	UTSW	9	67,163,233 (GRCm39)	missense	probably damaging	1.00
R8812:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8814:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8816:Tln2	UTSW	9	67,128,799 (GRCm39)	missense	probably damaging	1.00
R8816:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8833:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8835:Tln2	UTSW	9	67,304,975 (GRCm39)	splice site	probably benign
R8837:Tln2	UTSW	9	67,157,866 (GRCm39)	missense	probably damaging	0.99
R8843:Tln2	UTSW	9	67,302,827 (GRCm39)	missense	probably damaging	1.00
R8864:Tln2	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
R8867:Tln2	UTSW	9	67,237,832 (GRCm39)	missense	probably damaging	0.98
R8921:Tln2	UTSW	9	67,174,105 (GRCm39)	missense	probably damaging	0.99
R9080:Tln2	UTSW	9	67,253,843 (GRCm39)	missense	probably damaging	1.00
R9083:Tln2	UTSW	9	67,269,927 (GRCm39)	missense	probably damaging	0.96
R9150:Tln2	UTSW	9	67,128,778 (GRCm39)	missense	probably damaging	1.00
R9330:Tln2	UTSW	9	67,229,213 (GRCm39)	missense	possibly damaging	0.61
R9343:Tln2	UTSW	9	67,230,353 (GRCm39)	missense	probably benign	0.10
R9355:Tln2	UTSW	9	67,262,529 (GRCm39)	missense	possibly damaging	0.46
R9383:Tln2	UTSW	9	67,278,043 (GRCm39)	missense	probably benign	0.17
R9386:Tln2	UTSW	9	67,273,249 (GRCm39)	missense	possibly damaging	0.78
R9407:Tln2	UTSW	9	67,136,732 (GRCm39)	missense	probably damaging	1.00
R9483:Tln2	UTSW	9	67,299,769 (GRCm39)	missense	probably damaging	1.00
R9523:Tln2	UTSW	9	67,165,766 (GRCm39)	missense	probably damaging	0.99
R9642:Tln2	UTSW	9	67,157,826 (GRCm39)	missense	probably benign	0.02
R9703:Tln2	UTSW	9	67,293,938 (GRCm39)	missense	probably damaging	1.00
X0027:Tln2	UTSW	9	67,284,135 (GRCm39)	missense	probably damaging	1.00
X0064:Tln2	UTSW	9	67,255,420 (GRCm39)	missense	probably damaging	1.00
X0067:Tln2	UTSW	9	67,277,973 (GRCm39)	missense	probably damaging	1.00
Z1176:Tln2	UTSW	9	67,253,767 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- AAGCACTGTGTCACCTCCTC -3'
(R):5'- GTCTCAGTGCATCATTCACCTAGC -3'

Sequencing Primer
(F):5'- TCCATGCTGTCCCTTTAAGGTG -3'
(R):5'- TCATTCACCTAGCTTTCCTAAAAAG -3'

Posted On

2022-03-25