|Institutional Source||Beutler Lab|
|Gene Name||anaphase promoting complex subunit 4|
|Synonyms||2610306D21Rik, D5Ertd249e, APC4|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R9294 (G1)|
|Chromosomal Location||52834012-52867797 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 52864525 bp (GRCm38)|
|Amino Acid Change||Threonine to Alanine at position 650 (T650A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000031072 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000031072] [ENSMUST00000144574]|
AA Change: T650A
PolyPhen 2 Score 0.644 (Sensitivity: 0.87; Specificity: 0.91)
AA Change: T650A
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Anapc4||
(F):5'- GTCTAGCCGTTGATGTCAGG -3'
(R):5'- AGGATAACAGGAAAGCCTCTTG -3'
(F):5'- CAGGTTTTTAGAGCTGTGAATGAAC -3'
(R):5'- TCTTGCTCAATGAGGCCG -3'