Incidental Mutation 'R9307:Clock'
ID 705264
Institutional Source Beutler Lab
Gene Symbol Clock
Ensembl Gene ENSMUSG00000029238
Gene Name clock circadian regulator
Synonyms bHLHe8, KAT13D, 5330400M04Rik
MMRRC Submission
Accession Numbers
Essential gene? Possibly essential (E-score: 0.728) question?
Stock # R9307 (G1)
Quality Score 225.009
Status Validated
Chromosome 5
Chromosomal Location 76357715-76452639 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 76364671 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Phenylalanine to Leucine at position 815 (F815L)
Ref Sequence ENSEMBL: ENSMUSP00000074656 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000075159] [ENSMUST00000202122] [ENSMUST00000202651]
AlphaFold O08785
PDB Structure Crystal Structure of the Heterodimeric CLOCK:BMAL1 Transcriptional Activator Complex [X-RAY DIFFRACTION]
Predicted Effect unknown
Transcript: ENSMUST00000075159
AA Change: F815L
SMART Domains Protein: ENSMUSP00000074656
Gene: ENSMUSG00000029238
AA Change: F815L

DomainStartEndE-ValueType
HLH 40 90 7.77e-12 SMART
PAS 109 175 1.88e-6 SMART
PAS 264 330 3.65e-4 SMART
PAC 336 379 7.63e-7 SMART
low complexity region 426 446 N/A INTRINSIC
low complexity region 478 493 N/A INTRINSIC
coiled coil region 523 559 N/A INTRINSIC
low complexity region 619 634 N/A INTRINSIC
low complexity region 640 657 N/A INTRINSIC
low complexity region 738 796 N/A INTRINSIC
low complexity region 818 837 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000202122
AA Change: F814L
SMART Domains Protein: ENSMUSP00000144022
Gene: ENSMUSG00000029238
AA Change: F814L

DomainStartEndE-ValueType
TFS2N 34 106 4.1e-3 SMART
HLH 40 90 3.4e-14 SMART
PAS 109 175 9.6e-9 SMART
PAS 264 330 1.8e-6 SMART
PAC 336 379 3.9e-9 SMART
low complexity region 426 446 N/A INTRINSIC
low complexity region 478 493 N/A INTRINSIC
coiled coil region 523 559 N/A INTRINSIC
low complexity region 619 633 N/A INTRINSIC
low complexity region 639 656 N/A INTRINSIC
low complexity region 737 795 N/A INTRINSIC
low complexity region 817 836 N/A INTRINSIC
Predicted Effect unknown
Transcript: ENSMUST00000202651
AA Change: F815L
SMART Domains Protein: ENSMUSP00000143939
Gene: ENSMUSG00000029238
AA Change: F815L

DomainStartEndE-ValueType
HLH 40 90 7.77e-12 SMART
PAS 109 175 1.88e-6 SMART
PAS 264 330 3.65e-4 SMART
PAC 336 379 7.63e-7 SMART
low complexity region 426 446 N/A INTRINSIC
low complexity region 478 493 N/A INTRINSIC
coiled coil region 523 559 N/A INTRINSIC
low complexity region 619 634 N/A INTRINSIC
low complexity region 640 657 N/A INTRINSIC
low complexity region 738 796 N/A INTRINSIC
low complexity region 818 837 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.4%
  • 20x: 97.8%
Validation Efficiency 100% (64/64)
MGI Phenotype FUNCTION: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with Arntl (Bmal1) that binds E-box enhancer elements upstream of Period (Per1, Per2, Per3) and Cryptochrome (Cry1, Cry2) genes and activates transcription of these genes. Per and Cry proteins heterodimerize and repress their own transcription by interacting in a feedback loop with Clock/Arntl complexes. Polymorphisms in this gene may be associated with behavioral changes, obesity, and metabolic syndrome. Two transcripts encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2014]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal circadian phase. Mice homozygous for a spontaneous mutation exhibit abnormal circadian rhythm, reproduction, behavior, hair cycle, macronutrient absorption, and metabolism. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 64 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1110002E22Rik A C 3: 137,771,183 (GRCm39) D124A probably benign Het
Acd T C 8: 106,425,514 (GRCm39) D273G probably damaging Het
Acte1 G T 7: 143,434,902 (GRCm39) probably null Het
Adam15 T C 3: 89,254,790 (GRCm39) D90G possibly damaging Het
Adam2 A T 14: 66,287,921 (GRCm39) C392S probably damaging Het
Adam23 T A 1: 63,576,131 (GRCm39) N304K probably damaging Het
Adam34l A C 8: 44,079,304 (GRCm39) F307V probably benign Het
Aldh3b3 T C 19: 4,013,882 (GRCm39) F28L probably damaging Het
Bsn A G 9: 107,992,993 (GRCm39) S920P probably benign Het
Btc A G 5: 91,550,515 (GRCm39) probably null Het
Chchd2 A T 5: 129,916,054 (GRCm39) V12E probably benign Het
Chia1 A G 3: 106,035,991 (GRCm39) probably benign Het
Chil4 A T 3: 106,111,382 (GRCm39) probably null Het
Cmtr2 C T 8: 110,949,712 (GRCm39) T674M probably benign Het
Cnbd1 A C 4: 18,887,647 (GRCm39) I289R probably damaging Het
Cog2 A T 8: 125,253,837 (GRCm39) probably null Het
Col13a1 T C 10: 61,703,248 (GRCm39) I454V unknown Het
Cyct T A 2: 76,184,457 (GRCm39) Y98F probably benign Het
Dcaf6 T C 1: 165,227,236 (GRCm39) E297G possibly damaging Het
Dgka T C 10: 128,567,046 (GRCm39) H271R probably damaging Het
Dnah9 T A 11: 65,976,300 (GRCm39) I1250F probably benign Het
Dock4 T C 12: 40,686,155 (GRCm39) L130P probably damaging Het
Dpyd G A 3: 119,108,560 (GRCm39) V868I probably benign Het
Dync1li1 T A 9: 114,535,076 (GRCm39) D113E probably damaging Het
Fat3 T C 9: 15,932,719 (GRCm39) K1405E probably damaging Het
Fbn2 A G 18: 58,342,856 (GRCm39) C8R probably benign Het
Frmd4a A G 2: 4,609,044 (GRCm39) M971V probably benign Het
Glyatl3 C T 17: 41,223,553 (GRCm39) probably null Het
Grip1 A T 10: 119,821,454 (GRCm39) H373L probably benign Het
Ins1 T C 19: 52,253,258 (GRCm39) L66P possibly damaging Het
Kif21b A T 1: 136,101,800 (GRCm39) I1616L probably benign Het
Map7d1 A G 4: 126,128,024 (GRCm39) M637T unknown Het
Mc2r A T 18: 68,540,636 (GRCm39) M219K probably benign Het
Mcpt1 A G 14: 56,256,867 (GRCm39) D135G possibly damaging Het
Mturn T C 6: 54,676,541 (GRCm39) probably null Het
Mutyh T A 4: 116,674,074 (GRCm39) probably null Het
Myh3 A G 11: 66,984,397 (GRCm39) D1078G possibly damaging Het
Or4d5 T C 9: 40,012,451 (GRCm39) I112V probably benign Het
Pcdha12 A G 18: 37,153,874 (GRCm39) K198E probably damaging Het
Pcf11 A T 7: 92,306,534 (GRCm39) N1211K possibly damaging Het
Piezo1 G A 8: 123,213,832 (GRCm39) P1711S Het
Pkd1 T A 17: 24,769,451 (GRCm39) L72Q possibly damaging Het
Pramel20 C A 4: 143,299,314 (GRCm39) L326I probably damaging Het
Prss12 A G 3: 123,299,049 (GRCm39) D607G probably benign Het
R3hdml T C 2: 163,344,372 (GRCm39) *254R probably null Het
Rasal3 TTGGACCTGAGTGGA TTGGA 17: 32,612,502 (GRCm39) 782 probably null Het
Relch A T 1: 105,615,077 (GRCm39) Y272F probably benign Het
Rev3l C T 10: 39,693,149 (GRCm39) P410S probably benign Het
Sec14l2 A G 11: 4,068,665 (GRCm39) V5A probably benign Het
Slc12a3 A C 8: 95,061,625 (GRCm39) I291L probably benign Het
Slc4a10 T C 2: 62,083,662 (GRCm39) F372L probably damaging Het
Spta1 T C 1: 174,035,978 (GRCm39) W1095R probably damaging Het
Synj1 T C 16: 90,785,095 (GRCm39) T254A probably damaging Het
Taar2 T C 10: 23,817,237 (GRCm39) F259S probably damaging Het
Tns2 C A 15: 102,018,996 (GRCm39) L396I probably damaging Het
Tom1l1 TTGCTGCTGCTGCTGCTG TTGCTGCTGCTGCTG 11: 90,540,648 (GRCm39) probably benign Het
Traf5 T C 1: 191,747,033 (GRCm39) D96G probably damaging Het
Trgv3 A G 13: 19,427,441 (GRCm39) E108G probably damaging Het
Trpv6 A G 6: 41,602,378 (GRCm39) V336A probably benign Het
Usp45 A T 4: 21,824,998 (GRCm39) L583F probably damaging Het
Utrn T C 10: 12,554,475 (GRCm39) D1538G probably benign Het
Vmn1r209 T A 13: 22,990,072 (GRCm39) H206L probably damaging Het
Zfp51 T A 17: 21,684,733 (GRCm39) H449Q probably benign Het
Zfp653 G A 9: 21,969,321 (GRCm39) S315F possibly damaging Het
Other mutations in Clock
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00598:Clock APN 5 76,377,311 (GRCm39) missense probably benign 0.17
IGL00725:Clock APN 5 76,402,260 (GRCm39) nonsense probably null
IGL01304:Clock APN 5 76,414,202 (GRCm39) critical splice donor site probably null
IGL01369:Clock APN 5 76,384,933 (GRCm39) missense probably benign 0.30
IGL01542:Clock APN 5 76,379,322 (GRCm39) missense possibly damaging 0.82
IGL02541:Clock APN 5 76,410,519 (GRCm39) splice site probably null
IGL02602:Clock APN 5 76,402,274 (GRCm39) missense probably damaging 1.00
IGL02602:Clock APN 5 76,402,273 (GRCm39) missense probably null 1.00
IGL03186:Clock APN 5 76,390,929 (GRCm39) missense probably damaging 0.98
IGL03309:Clock APN 5 76,379,241 (GRCm39) critical splice donor site probably null
R6760_Clock_188 UTSW 5 76,374,823 (GRCm39) missense unknown
uhr UTSW 5 76,377,401 (GRCm39) nonsense probably null
R0304:Clock UTSW 5 76,374,832 (GRCm39) missense unknown
R0593:Clock UTSW 5 76,413,683 (GRCm39) missense probably benign 0.25
R0654:Clock UTSW 5 76,374,976 (GRCm39) missense possibly damaging 0.95
R0684:Clock UTSW 5 76,393,365 (GRCm39) missense probably damaging 0.96
R0707:Clock UTSW 5 76,374,976 (GRCm39) missense possibly damaging 0.95
R0751:Clock UTSW 5 76,377,208 (GRCm39) missense possibly damaging 0.75
R0865:Clock UTSW 5 76,414,271 (GRCm39) splice site probably benign
R0920:Clock UTSW 5 76,378,167 (GRCm39) missense possibly damaging 0.80
R1396:Clock UTSW 5 76,414,649 (GRCm39) missense probably benign 0.00
R1450:Clock UTSW 5 76,410,578 (GRCm39) nonsense probably null
R1487:Clock UTSW 5 76,414,201 (GRCm39) splice site probably null
R1574:Clock UTSW 5 76,390,679 (GRCm39) missense probably damaging 1.00
R1574:Clock UTSW 5 76,390,679 (GRCm39) missense probably damaging 1.00
R1858:Clock UTSW 5 76,388,756 (GRCm39) missense possibly damaging 0.92
R1872:Clock UTSW 5 76,396,309 (GRCm39) missense possibly damaging 0.67
R1905:Clock UTSW 5 76,414,735 (GRCm39) splice site probably benign
R1937:Clock UTSW 5 76,377,340 (GRCm39) missense probably damaging 0.99
R2411:Clock UTSW 5 76,379,360 (GRCm39) missense probably benign 0.08
R2887:Clock UTSW 5 76,393,120 (GRCm39) missense probably damaging 0.99
R3410:Clock UTSW 5 76,377,401 (GRCm39) nonsense probably null
R4514:Clock UTSW 5 76,378,046 (GRCm39) missense probably benign 0.00
R4598:Clock UTSW 5 76,383,657 (GRCm39) missense probably benign 0.00
R4599:Clock UTSW 5 76,383,657 (GRCm39) missense probably benign 0.00
R4795:Clock UTSW 5 76,413,763 (GRCm39) missense probably damaging 1.00
R4796:Clock UTSW 5 76,413,763 (GRCm39) missense probably damaging 1.00
R4973:Clock UTSW 5 76,402,258 (GRCm39) missense possibly damaging 0.62
R5204:Clock UTSW 5 76,391,017 (GRCm39) splice site probably null
R5271:Clock UTSW 5 76,389,801 (GRCm39) missense probably damaging 1.00
R5547:Clock UTSW 5 76,378,185 (GRCm39) missense probably benign 0.02
R5630:Clock UTSW 5 76,378,185 (GRCm39) missense probably benign 0.02
R5631:Clock UTSW 5 76,378,185 (GRCm39) missense probably benign 0.02
R5632:Clock UTSW 5 76,378,185 (GRCm39) missense probably benign 0.02
R5787:Clock UTSW 5 76,384,898 (GRCm39) missense probably damaging 1.00
R6274:Clock UTSW 5 76,385,000 (GRCm39) missense probably benign 0.45
R6578:Clock UTSW 5 76,364,556 (GRCm39) missense unknown
R6622:Clock UTSW 5 76,389,801 (GRCm39) missense probably damaging 1.00
R6760:Clock UTSW 5 76,374,823 (GRCm39) missense unknown
R6793:Clock UTSW 5 76,384,967 (GRCm39) frame shift probably null
R7406:Clock UTSW 5 76,414,692 (GRCm39) start codon destroyed probably null 0.26
R7414:Clock UTSW 5 76,410,611 (GRCm39) missense probably benign 0.00
R7560:Clock UTSW 5 76,390,738 (GRCm39) splice site probably null
R7593:Clock UTSW 5 76,384,145 (GRCm39) missense possibly damaging 0.80
R7640:Clock UTSW 5 76,396,225 (GRCm39) missense possibly damaging 0.71
R7708:Clock UTSW 5 76,414,256 (GRCm39) missense probably benign 0.00
R7713:Clock UTSW 5 76,393,267 (GRCm39) critical splice donor site probably null
R7807:Clock UTSW 5 76,390,982 (GRCm39) missense probably benign 0.01
R8171:Clock UTSW 5 76,414,261 (GRCm39) missense possibly damaging 0.94
R8190:Clock UTSW 5 76,375,051 (GRCm39) missense probably damaging 0.98
R8225:Clock UTSW 5 76,389,759 (GRCm39) missense probably damaging 0.99
R8309:Clock UTSW 5 76,402,269 (GRCm39) missense probably benign 0.07
R8557:Clock UTSW 5 76,377,217 (GRCm39) missense probably damaging 1.00
R8792:Clock UTSW 5 76,410,574 (GRCm39) missense probably damaging 1.00
R8869:Clock UTSW 5 76,374,889 (GRCm39) small deletion probably benign
R8870:Clock UTSW 5 76,383,632 (GRCm39) missense probably benign 0.17
R8980:Clock UTSW 5 76,402,286 (GRCm39) missense probably benign 0.01
R8982:Clock UTSW 5 76,364,559 (GRCm39) missense unknown
R9177:Clock UTSW 5 76,377,256 (GRCm39) missense probably benign 0.00
R9208:Clock UTSW 5 76,384,871 (GRCm39) missense probably benign 0.00
R9213:Clock UTSW 5 76,393,376 (GRCm39) missense possibly damaging 0.94
R9446:Clock UTSW 5 76,396,288 (GRCm39) missense probably benign 0.00
R9516:Clock UTSW 5 76,377,227 (GRCm39) missense possibly damaging 0.85
R9572:Clock UTSW 5 76,377,338 (GRCm39) missense probably benign 0.00
R9630:Clock UTSW 5 76,393,281 (GRCm39) missense probably benign 0.03
Predicted Primers PCR Primer
(F):5'- GAGCGATTCTTTCTGGCCATCC -3'
(R):5'- TCTTACGTGTTCAGAAAGAAGGC -3'

Sequencing Primer
(F):5'- CCCTTCCTCTCGCATGTCAGAG -3'
(R):5'- GAAAGAAGGCAAGGTTTTTGTTTTCC -3'
Posted On 2022-03-25