Mutagenetix > Incidental Mutation

Incidental Mutation 'R9311:Ifi204'

705528

Institutional Source

Beutler Lab

Gene Symbol

Ifi204

Ensembl Gene

ENSMUSG00000073489

Gene Name

interferon activated gene 204

Synonyms

p204

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.166) question?

Stock #

R9311 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

173574859-173594509 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 173589215 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 72 (V72A)

Ref Sequence

ENSEMBL: ENSMUSP00000106845 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111214]

AlphaFold

P0DOV2

Predicted Effect

possibly damaging
Transcript: ENSMUST00000111214
AA Change: V72A

PolyPhen 2 Score 0.454 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000106845
Gene: ENSMUSG00000073489
AA Change: V72A

Domain	Start	End	E-Value	Type
PYRIN	6	84	8.33e-14	SMART
low complexity region	120	154	N/A	INTRINSIC
low complexity region	190	206	N/A	INTRINSIC
Pfam:HIN	225	393	6.2e-78	PFAM
Pfam:HIN	429	595	9.8e-78	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (61/61)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4gnt	T	C	9: 99,495,816 (GRCm39)	I84T	possibly damaging	Het
Adad2	G	T	8: 120,341,986 (GRCm39)	R268L	probably damaging	Het
Agxt2	A	T	15: 10,380,733 (GRCm39)	N208I	probably damaging	Het
Brsk1	G	T	7: 4,709,722 (GRCm39)		probably null	Het
Cd40	A	T	2: 164,912,667 (GRCm39)	Q235L	possibly damaging	Het
Cdcp3	A	T	7: 130,859,490 (GRCm39)	D1137V	unknown	Het
Cln6	T	C	9: 62,757,900 (GRCm39)	Y220H	probably damaging	Het
Cngb1	A	T	8: 96,010,794 (GRCm39)		probably null	Het
Cpne1	A	T	2: 155,919,723 (GRCm39)	V277E	probably damaging	Het
Csf2rb2	C	T	15: 78,176,735 (GRCm39)		probably null	Het
Cyp4f15	A	G	17: 32,905,139 (GRCm39)	T41A	probably benign	Het
Dab1	T	C	4: 104,369,463 (GRCm39)		probably null	Het
Eif4e1b	C	A	13: 54,932,332 (GRCm39)	H56N	probably benign	Het
Elp3	A	T	14: 65,823,788 (GRCm39)	D78E	probably benign	Het
Ephx3	C	G	17: 32,408,290 (GRCm39)	D45H	probably benign	Het
Gabarap	T	A	11: 69,882,549 (GRCm39)	V4E	probably benign	Het
Gm5105	T	A	3: 137,755,418 (GRCm39)	D56V	unknown	Het
Gosr2	G	T	11: 103,574,693 (GRCm39)	H134Q	probably damaging	Het
Irx1	A	G	13: 72,107,416 (GRCm39)	V422A	probably benign	Het
Kcne4	C	A	1: 78,795,824 (GRCm39)	D157E	probably benign	Het
Kctd13	A	G	7: 126,541,345 (GRCm39)	N195S	probably damaging	Het
Kirrel1	A	G	3: 87,005,123 (GRCm39)	V75A	probably benign	Het
Klkb1	T	C	8: 45,722,983 (GRCm39)	T625A	probably benign	Het
Lama5	A	G	2: 179,838,275 (GRCm39)		probably null	Het
Lifr	A	G	15: 7,208,418 (GRCm39)	I599V	possibly damaging	Het
Lin52	A	T	12: 84,576,470 (GRCm39)	E101V	probably damaging	Het
Liph	T	C	16: 21,802,680 (GRCm39)	I130V	probably benign	Het
Liph	C	T	16: 21,774,913 (GRCm39)	R428Q	probably damaging	Het
Lrrc4c	A	C	2: 97,461,080 (GRCm39)	I569L	possibly damaging	Het
Mnat1	G	A	12: 73,214,916 (GRCm39)	V78I	probably benign	Het
Msln	C	T	17: 25,971,990 (GRCm39)	D76N	probably benign	Het
Myh7b	A	G	2: 155,463,253 (GRCm39)	H495R	probably damaging	Het
Ndst4	C	T	3: 125,518,385 (GRCm39)	S354L	probably benign	Het
Nfu1	T	C	6: 86,986,926 (GRCm39)	V15A	probably benign	Het
Nphp4	T	C	4: 152,608,714 (GRCm39)	S441P	probably damaging	Het
Nuak1	T	C	10: 84,214,090 (GRCm39)		probably null	Het
Or1j20	A	T	2: 36,760,405 (GRCm39)	I276F	probably damaging	Het
Or5h27	A	T	16: 59,006,106 (GRCm39)	C247S	unknown	Het
Or5w22	A	G	2: 87,362,358 (GRCm39)		probably benign	Het
Or8b3b	A	G	9: 38,583,925 (GRCm39)	S272P	probably damaging	Het
Palld	A	G	8: 61,978,189 (GRCm39)	V1109A	unknown	Het
Pik3c3	G	A	18: 30,445,666 (GRCm39)	R551H	probably benign	Het
Plcb1	G	A	2: 135,189,385 (GRCm39)	V838I	probably benign	Het
Plppr4	G	T	3: 117,119,518 (GRCm39)	T297K	probably damaging	Het
Ptprd	A	G	4: 76,051,320 (GRCm39)	I67T	probably benign	Het
Rb1cc1	T	A	1: 6,310,539 (GRCm39)	N312K	probably damaging	Het
Sh3glb1	A	C	3: 144,397,659 (GRCm39)		probably null	Het
Siglec1	C	T	2: 130,916,013 (GRCm39)	C1283Y	probably damaging	Het
Spns1	T	C	7: 125,972,995 (GRCm39)	I204V	probably damaging	Het
Sprr5	T	G	3: 92,440,397 (GRCm39)	Q14P	unknown	Het
Supt6	T	C	11: 78,116,284 (GRCm39)	Y693C	probably damaging	Het
Taar9	A	G	10: 23,985,152 (GRCm39)	V94A	probably damaging	Het
Tchp	T	C	5: 114,846,877 (GRCm39)	S55P	probably benign	Het
Tmem132b	A	G	5: 125,863,029 (GRCm39)	H678R	possibly damaging	Het
Tnr	G	C	1: 159,677,663 (GRCm39)	G16A	probably benign	Het
Top3b	T	C	16: 16,700,563 (GRCm39)		probably null	Het
Tpmt	A	G	13: 47,185,892 (GRCm39)		probably null	Het
Treh	G	A	9: 44,592,655 (GRCm39)	V87I	probably benign	Het
Ttc4	T	C	4: 106,535,963 (GRCm39)	D33G	probably benign	Het
Usp47	A	G	7: 111,703,257 (GRCm39)	D1171G	probably benign	Het
Vmn2r61	G	T	7: 41,950,092 (GRCm39)	L837F	possibly damaging	Het
Vmn2r86	T	A	10: 130,288,440 (GRCm39)	N354Y	probably damaging	Het
Vmn2r88	C	G	14: 51,650,503 (GRCm39)	A72G	probably benign	Het
Zscan4b	C	A	7: 10,635,950 (GRCm39)	V126F	probably damaging	Het

Other mutations in Ifi204

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00906:Ifi204	APN	1	173,587,197 (GRCm39)	splice site	probably benign
IGL01922:Ifi204	APN	1	173,589,288 (GRCm39)	missense	possibly damaging	0.51
IGL02296:Ifi204	APN	1	173,576,880 (GRCm39)	missense	possibly damaging	0.93
IGL02419:Ifi204	APN	1	173,576,946 (GRCm39)	missense	possibly damaging	0.71
IGL02505:Ifi204	APN	1	173,583,220 (GRCm39)	missense	probably benign	0.04
R0938:Ifi204	UTSW	1	173,579,311 (GRCm39)	missense	possibly damaging	0.85
R1363:Ifi204	UTSW	1	173,576,862 (GRCm39)	missense	probably benign	0.00
R1834:Ifi204	UTSW	1	173,575,172 (GRCm39)	missense	unknown
R2031:Ifi204	UTSW	1	173,580,343 (GRCm39)	missense	probably damaging	1.00
R2254:Ifi204	UTSW	1	173,589,296 (GRCm39)	missense	possibly damaging	0.95
R2379:Ifi204	UTSW	1	173,583,559 (GRCm39)	nonsense	probably null
R2408:Ifi204	UTSW	1	173,583,198 (GRCm39)	missense	possibly damaging	0.80
R3011:Ifi204	UTSW	1	173,579,217 (GRCm39)	missense	probably benign	0.01
R3617:Ifi204	UTSW	1	173,583,283 (GRCm39)	missense	possibly damaging	0.51
R3894:Ifi204	UTSW	1	173,576,774 (GRCm39)	missense	possibly damaging	0.86
R3916:Ifi204	UTSW	1	173,583,341 (GRCm39)	missense	possibly damaging	0.95
R4656:Ifi204	UTSW	1	173,587,927 (GRCm39)	intron	probably benign
R4657:Ifi204	UTSW	1	173,587,927 (GRCm39)	intron	probably benign
R4694:Ifi204	UTSW	1	173,576,825 (GRCm39)	missense	probably damaging	0.99
R4703:Ifi204	UTSW	1	173,587,927 (GRCm39)	intron	probably benign
R4704:Ifi204	UTSW	1	173,587,927 (GRCm39)	intron	probably benign
R4894:Ifi204	UTSW	1	173,587,808 (GRCm39)	missense	probably damaging	0.98
R4947:Ifi204	UTSW	1	173,583,316 (GRCm39)	missense	probably damaging	0.98
R5023:Ifi204	UTSW	1	173,579,306 (GRCm39)	missense	possibly damaging	0.93
R5036:Ifi204	UTSW	1	173,580,311 (GRCm39)	missense	possibly damaging	0.79
R5119:Ifi204	UTSW	1	173,583,234 (GRCm39)	missense	probably damaging	1.00
R5194:Ifi204	UTSW	1	173,576,910 (GRCm39)	missense	possibly damaging	0.86
R5762:Ifi204	UTSW	1	173,580,325 (GRCm39)	missense	probably damaging	0.98
R6063:Ifi204	UTSW	1	173,579,223 (GRCm39)	missense	probably benign	0.03
R6808:Ifi204	UTSW	1	173,589,269 (GRCm39)	missense	probably benign	0.27
R7311:Ifi204	UTSW	1	173,587,134 (GRCm39)	missense	probably benign	0.26
R7338:Ifi204	UTSW	1	173,587,703 (GRCm39)	missense	possibly damaging	0.67
R7430:Ifi204	UTSW	1	173,583,247 (GRCm39)	missense	probably benign	0.43
R7528:Ifi204	UTSW	1	173,579,406 (GRCm39)	missense	probably benign	0.06
R7985:Ifi204	UTSW	1	173,587,772 (GRCm39)	missense	possibly damaging	0.50
R8021:Ifi204	UTSW	1	173,586,919 (GRCm39)	intron	probably benign
R8137:Ifi204	UTSW	1	173,589,188 (GRCm39)	missense	possibly damaging	0.65
R8141:Ifi204	UTSW	1	173,583,189 (GRCm39)	missense	possibly damaging	0.81
R8191:Ifi204	UTSW	1	173,579,226 (GRCm39)	missense	possibly damaging	0.71
R8487:Ifi204	UTSW	1	173,587,839 (GRCm39)	missense	probably damaging	0.99
R9075:Ifi204	UTSW	1	173,589,282 (GRCm39)	missense	possibly damaging	0.95
R9124:Ifi204	UTSW	1	173,579,193 (GRCm39)	critical splice donor site	probably null
R9498:Ifi204	UTSW	1	173,583,537 (GRCm39)	missense	possibly damaging	0.81
R9712:Ifi204	UTSW	1	173,576,924 (GRCm39)	missense	probably damaging	0.99
Z1176:Ifi204	UTSW	1	173,579,194 (GRCm39)	missense	probably null	0.00

Predicted Primers

PCR Primer
(F):5'- AGACAAACGTCCTCAGTAGC -3'
(R):5'- AGAGAATTGTTCTGCTGAGAGGAC -3'

Sequencing Primer
(F):5'- CGTCCTCAGTAGCTAATCCAAAG -3'
(R):5'- CTGCTGAGAGGACTTGAATGTATC -3'

Posted On

2022-03-25