Mutagenetix > Incidental Mutation

Incidental Mutation 'R9312:Fer1l5'

705627

Institutional Source

Beutler Lab

Gene Symbol

Fer1l5

Ensembl Gene

ENSMUSG00000037432

Gene Name

fer-1 like family member 5

Synonyms

4930533C12Rik

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R9312 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

36411372-36461191 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 36460248 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 1910 (E1910G)

Ref Sequence

ENSEMBL: ENSMUSP00000142130 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000001171] [ENSMUST00000115011] [ENSMUST00000125304] [ENSMUST00000140452] [ENSMUST00000179162]

AlphaFold

P0DM40

Predicted Effect

probably benign
Transcript: ENSMUST00000001171

SMART Domains

Protein: ENSMUSP00000137028
Gene: ENSMUSG00000001143

Domain	Start	End	E-Value	Type
signal peptide	1	33	N/A	INTRINSIC
Pfam:Lectin_leg-like	48	146	1.5e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000115011

SMART Domains

Protein: ENSMUSP00000110663
Gene: ENSMUSG00000001143

Domain	Start	End	E-Value	Type
signal peptide	1	33	N/A	INTRINSIC
Pfam:Lectin_leg-like	48	286	2e-84	PFAM
transmembrane domain	324	346	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000125304

SMART Domains

Protein: ENSMUSP00000117200
Gene: ENSMUSG00000001143

Domain	Start	End	E-Value	Type
signal peptide	1	33	N/A	INTRINSIC
Pfam:Lectin_leg-like	48	275	3.2e-88	PFAM
transmembrane domain	313	335	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000140452

SMART Domains

Protein: ENSMUSP00000144430
Gene: ENSMUSG00000037432

Domain	Start	End	E-Value	Type
Pfam:C2	1	78	1.4e-5	PFAM
Blast:C2	148	209	3e-37	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000152088

SMART Domains

Protein: ENSMUSP00000119798
Gene: ENSMUSG00000001143

Domain	Start	End	E-Value	Type
Pfam:Lectin_leg-like	1	76	1.3e-22	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000179162
AA Change: E1910G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000142130
Gene: ENSMUSG00000037432
AA Change: E1910G

Domain	Start	End	E-Value	Type
C2	1	98	2.74e-4	SMART
C2	168	264	4.29e-6	SMART
FerI	250	323	1.59e-19	SMART
C2	325	422	1.06e-5	SMART
FerA	602	669	6.26e-18	SMART
FerB	691	764	1.38e-37	SMART
internal_repeat_1	781	836	1.77e-5	PROSPERO
internal_repeat_1	852	904	1.77e-5	PROSPERO
DysFC	913	951	1.61e-3	SMART
DysFC	981	1013	4.81e-2	SMART
C2	1078	1222	1.56e0	SMART
Pfam:C2	1248	1329	1e-1	PFAM
low complexity region	1376	1387	N/A	INTRINSIC
low complexity region	1459	1470	N/A	INTRINSIC
C2	1487	1586	2.21e-8	SMART
C2	1659	1851	5.32e-2	SMART
transmembrane domain	1964	1986	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000192969

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam8	A	G	7: 139,565,791 (GRCm39)	L607P	probably damaging	Het
Adamts16	T	A	13: 70,949,045 (GRCm39)	R205S	probably damaging	Het
Adgra3	T	C	5: 50,117,900 (GRCm39)	Y1216C	probably damaging	Het
Arl3	A	G	19: 46,531,964 (GRCm39)	V91A	probably damaging	Het
Bckdhb	A	T	9: 83,870,833 (GRCm39)	K114N	probably benign	Het
Cabin1	A	T	10: 75,561,569 (GRCm39)	D150E	probably benign	Het
Cadps	G	A	14: 12,616,095 (GRCm38)	R312C	probably damaging	Het
Cep120	A	G	18: 53,860,713 (GRCm39)	I239T	probably benign	Het
Clec4g	T	A	8: 3,768,371 (GRCm39)	E120V	probably null	Het
Col18a1	T	C	10: 76,894,606 (GRCm39)	E1022G	probably damaging	Het
Cyfip2	A	G	11: 46,167,709 (GRCm39)	I231T	possibly damaging	Het
Dcaf17	T	C	2: 70,908,458 (GRCm39)	Y228H	probably benign	Het
Ddx41	T	A	13: 55,683,842 (GRCm39)	Q52L	probably benign	Het
Dync2h1	T	C	9: 7,050,413 (GRCm39)	Y3212C	probably damaging	Het
Etv4	A	G	11: 101,664,923 (GRCm39)	Y177H	probably benign	Het
Fgf11	G	T	11: 69,689,412 (GRCm39)	H195Q	probably damaging	Het
Gatb	A	G	3: 85,561,070 (GRCm39)	K529E	probably damaging	Het
Gfus	T	C	15: 75,797,169 (GRCm39)	Y292C	possibly damaging	Het
Gsdmc	C	A	15: 63,649,806 (GRCm39)	A362S	probably damaging	Het
Gucy1b1	A	T	3: 81,942,123 (GRCm39)	N545K	probably damaging	Het
Heatr1	A	G	13: 12,446,565 (GRCm39)	H1638R	probably benign	Het
Hecw1	T	G	13: 14,546,567 (GRCm39)	I145L	probably damaging	Het
Hmmr	A	G	11: 40,614,316 (GRCm39)	V124A	possibly damaging	Het
Il31ra	C	T	13: 112,686,023 (GRCm39)	V89I	probably benign	Het
Kcnmb3	A	T	3: 32,536,575 (GRCm39)	M51K	probably benign	Het
Lamb1	C	T	12: 31,368,352 (GRCm39)	R1209C	probably damaging	Het
Lcn6	G	T	2: 25,570,074 (GRCm39)	G80V	probably benign	Het
Lgsn	A	G	1: 31,243,280 (GRCm39)	E454G	probably benign	Het
Loxhd1	G	A	18: 77,498,285 (GRCm39)	M1624I	probably benign	Het
Ltbp2	G	A	12: 84,837,864 (GRCm39)	P1192L	probably benign	Het
Mapk8ip3	T	C	17: 25,146,925 (GRCm39)		probably null	Het
Mlip	A	G	9: 77,147,060 (GRCm39)	Y26H	probably damaging	Het
Nipsnap1	A	C	11: 4,839,902 (GRCm39)	I208L	possibly damaging	Het
Nusap1	CGAGGAGGAGGAGGAGGA	CGAGGAGGAGGAGGA	2: 119,458,119 (GRCm39)		probably benign	Het
Or5m11	T	A	2: 85,781,509 (GRCm39)	V34D	possibly damaging	Het
Pigr	C	T	1: 130,762,185 (GRCm39)	T7M	probably benign	Het
Pigu	T	C	2: 155,199,315 (GRCm39)	M1V	probably null	Het
Pkd1	T	A	17: 24,797,364 (GRCm39)	I2568N	probably damaging	Het
Psmd4	A	T	3: 94,940,729 (GRCm39)	S345T	probably benign	Het
Rab3gap2	A	T	1: 185,015,684 (GRCm39)	M1321L	probably benign	Het
Rnf157	A	G	11: 116,240,158 (GRCm39)		probably null	Het
Sart3	C	T	5: 113,900,935 (GRCm39)	E168K	possibly damaging	Het
Scnn1g	A	G	7: 121,339,818 (GRCm39)	K206E	probably benign	Het
Smg6	A	C	11: 74,820,877 (GRCm39)	S383R	probably benign	Het
Smurf1	G	T	5: 144,830,893 (GRCm39)	P294H	probably damaging	Het
Sspo	A	T	6: 48,445,396 (GRCm39)	H2279L	probably benign	Het
Tacc2	A	T	7: 130,223,978 (GRCm39)	D221V	probably benign	Het
Tgm6	T	A	2: 129,977,701 (GRCm39)	H13Q	probably benign	Het
Tnfaip8l2	T	C	3: 95,047,767 (GRCm39)	D32G	probably damaging	Het
Tppp	A	G	13: 74,179,377 (GRCm39)	D167G	probably damaging	Het
Trak1	A	G	9: 121,280,757 (GRCm39)	E382G	probably benign	Het
Trdv2-1	C	T	14: 54,183,570 (GRCm39)	T14I	probably benign	Het
Ubr3	A	T	2: 69,784,677 (GRCm39)	D799V	probably damaging	Het
Usp28	T	A	9: 48,926,439 (GRCm39)	Y317*	probably null	Het
Vmn1r225	C	T	17: 20,722,960 (GRCm39)	L134F	probably benign	Het
Vmn2r86	A	G	10: 130,288,406 (GRCm39)	I365T	probably benign	Het
Wfikkn2	A	G	11: 94,129,497 (GRCm39)	S215P	probably damaging	Het
Xrcc2	A	G	5: 25,897,124 (GRCm39)	V275A	probably damaging	Het
Zfp423	T	A	8: 88,508,569 (GRCm39)	I592F	probably damaging	Het

Other mutations in Fer1l5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
PIT4151001:Fer1l5	UTSW	1	36,450,728 (GRCm39)	missense	probably benign	0.27
R5580:Fer1l5	UTSW	1	36,424,539 (GRCm39)	nonsense	probably null
R5848:Fer1l5	UTSW	1	36,428,016 (GRCm39)	missense	probably benign	0.39
R5930:Fer1l5	UTSW	1	36,424,254 (GRCm39)	nonsense	probably null
R6193:Fer1l5	UTSW	1	36,448,517 (GRCm39)	missense	probably benign	0.20
R6195:Fer1l5	UTSW	1	36,414,367 (GRCm39)	splice site	probably null
R6207:Fer1l5	UTSW	1	36,424,241 (GRCm39)	missense	probably damaging	1.00
R6233:Fer1l5	UTSW	1	36,414,367 (GRCm39)	splice site	probably null
R6349:Fer1l5	UTSW	1	36,450,355 (GRCm39)	missense	probably damaging	0.96
R6478:Fer1l5	UTSW	1	36,441,612 (GRCm39)	missense	probably damaging	1.00
R6514:Fer1l5	UTSW	1	36,442,697 (GRCm39)	missense	probably benign	0.01
R6611:Fer1l5	UTSW	1	36,445,735 (GRCm39)	missense	probably benign	0.01
R6634:Fer1l5	UTSW	1	36,450,466 (GRCm39)	missense	probably damaging	0.99
R6733:Fer1l5	UTSW	1	36,447,753 (GRCm39)	critical splice donor site	probably null
R6816:Fer1l5	UTSW	1	36,445,591 (GRCm39)	missense	possibly damaging	0.60
R7225:Fer1l5	UTSW	1	36,460,033 (GRCm39)	missense	possibly damaging	0.90
R7316:Fer1l5	UTSW	1	36,457,197 (GRCm39)	missense	probably benign	0.41
R7455:Fer1l5	UTSW	1	36,428,064 (GRCm39)	missense	probably benign	0.00
R7473:Fer1l5	UTSW	1	36,460,689 (GRCm39)	missense	possibly damaging	0.53
R7702:Fer1l5	UTSW	1	36,459,775 (GRCm39)	nonsense	probably null
R7714:Fer1l5	UTSW	1	36,440,558 (GRCm39)	missense	probably damaging	1.00
R7872:Fer1l5	UTSW	1	36,460,967 (GRCm39)	missense	probably benign	0.00
R7881:Fer1l5	UTSW	1	36,446,117 (GRCm39)	missense	not run
R7984:Fer1l5	UTSW	1	36,447,702 (GRCm39)	missense	possibly damaging	0.68
R8326:Fer1l5	UTSW	1	36,415,841 (GRCm39)	missense	probably benign	0.04
R8523:Fer1l5	UTSW	1	36,426,271 (GRCm39)	missense	probably benign	0.27
R8528:Fer1l5	UTSW	1	36,456,855 (GRCm39)	missense	possibly damaging	0.91
R8975:Fer1l5	UTSW	1	36,456,897 (GRCm39)	missense	probably benign	0.13
R9011:Fer1l5	UTSW	1	36,441,601 (GRCm39)	missense	probably damaging	0.96
R9084:Fer1l5	UTSW	1	36,429,619 (GRCm39)	missense	probably benign	0.00
R9140:Fer1l5	UTSW	1	36,460,047 (GRCm39)	intron	probably benign
R9180:Fer1l5	UTSW	1	36,449,999 (GRCm39)	missense	probably null	1.00
R9510:Fer1l5	UTSW	1	36,442,662 (GRCm39)	missense	probably damaging	0.97
R9655:Fer1l5	UTSW	1	36,460,696 (GRCm39)	missense	probably benign	0.40
Z1176:Fer1l5	UTSW	1	36,429,644 (GRCm39)	nonsense	probably null
Z1177:Fer1l5	UTSW	1	36,448,275 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- CTCTCTAGCAAACTTCTGGGG -3'
(R):5'- GGAGTCCCTTACACTCAACC -3'

Sequencing Primer
(F):5'- CTGCAGCCAAACCTCTT -3'
(R):5'- TCTGATGTCAACTAAACCAGGTAGG -3'

Posted On

2022-03-25