Mutagenetix > Incidental Mutations

Incidental Mutation 'R8895:Tmem260'

706132

Institutional Source

Beutler Lab

Gene Symbol

Tmem260

Ensembl Gene

ENSMUSG00000036339

Gene Name

transmembrane protein 260

Synonyms

6720456H20Rik

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R8895 (G1)

Quality Score

137.008

Status

Validated

Chromosome

Chromosomal Location

48446124-48524246 bp(+) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

A to G at 48500388 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000153822 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111735] [ENSMUST00000124720] [ENSMUST00000153765] [ENSMUST00000226422] [ENSMUST00000227440] [ENSMUST00000228697]

AlphaFold

Q8BMD6

Predicted Effect

probably benign
Transcript: ENSMUST00000111735

SMART Domains

Protein: ENSMUSP00000107364
Gene: ENSMUSG00000036339

Domain	Start	End	E-Value	Type
transmembrane domain	21	40	N/A	INTRINSIC
Pfam:DUF2723	48	211	1.9e-43	PFAM
transmembrane domain	316	338	N/A	INTRINSIC
transmembrane domain	353	372	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124720

SMART Domains

Protein: ENSMUSP00000118376
Gene: ENSMUSG00000036339

Domain	Start	End	E-Value	Type
Pfam:DUF2723	1	61	4.6e-9	PFAM
transmembrane domain	65	87	N/A	INTRINSIC
transmembrane domain	164	186	N/A	INTRINSIC
transmembrane domain	201	220	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000153765

SMART Domains

Protein: ENSMUSP00000116155
Gene: ENSMUSG00000036339

Domain	Start	End	E-Value	Type
transmembrane domain	21	40	N/A	INTRINSIC
Pfam:DUF2723	48	111	8.3e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000226422

Predicted Effect

probably benign
Transcript: ENSMUST00000227440

Predicted Effect

probably benign
Transcript: ENSMUST00000228697

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (74/74)

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930567H17Rik	G	A	X: 70,394,388	R100W	possibly damaging	Het
4931409K22Rik	T	C	5: 24,550,630	D298G	probably benign	Het
5830411N06Rik	C	T	7: 140,261,619	P279S	possibly damaging	Het
Adamts10	G	A	17: 33,549,296	R814H	probably damaging	Het
Akr1a1	A	G	4: 116,641,063	L95P	probably damaging	Het
Amigo2	G	T	15: 97,245,508	N344K	probably damaging	Het
Ankrd11	T	C	8: 122,894,275	D946G	possibly damaging	Het
Btnl6	T	G	17: 34,515,417	I124L	probably benign	Het
Bzw2	A	T	12: 36,123,983	C97*	probably null	Het
Celsr2	G	A	3: 108,413,564	T644I	possibly damaging	Het
Cep85l	A	T	10: 53,348,815	L226Q	possibly damaging	Het
Ces1g	T	C	8: 93,319,884	K338E	possibly damaging	Het
Cldn10	G	A	14: 118,855,095	D36N	probably damaging	Het
Cntnap4	T	A	8: 112,752,966	I261N	probably benign	Het
Csnk1g1	T	C	9: 66,007,827		probably null	Het
Cyp2c69	T	C	19: 39,881,466	D104G	possibly damaging	Het
Cyp3a11	T	C	5: 145,860,520	T350A	probably benign	Het
Daw1	T	A	1: 83,209,290	C274S	probably damaging	Het
Dmtn	T	C	14: 70,612,704	T267A	probably benign	Het
Dock5	A	G	14: 67,817,663	Y585H	possibly damaging	Het
Dsg2	T	G	18: 20,590,069	V384G	probably damaging	Het
Elmo1	C	T	13: 20,564,460	L492F	probably damaging	Het
Fam172a	T	A	13: 77,999,654	N280K	probably damaging	Het
Fmo1	T	C	1: 162,830,258	D438G	probably benign	Het
Folr2	C	T	7: 101,840,201	V244M	unknown	Het
Galnt18	T	C	7: 111,779,502	I16V	possibly damaging	Het
Gm10036	C	A	18: 15,833,150	Y119*	probably null	Het
Gm853	T	C	4: 130,211,430	K316E	probably benign	Het
Gm884	T	A	11: 103,618,830	T771S	unknown	Het
Gria4	A	G	9: 4,664,951	S102P	probably damaging	Het
Hc	A	T	2: 35,000,849	N1318K	probably benign	Het
Ifitm1	T	C	7: 140,969,586	L94P	probably damaging	Het
Itga9	G	A	9: 118,681,767	V455M	probably damaging	Het
Kank4	C	T	4: 98,765,510	V894I	possibly damaging	Het
Kcnip2	T	A	19: 45,796,661		probably benign	Het
Lamb1	A	G	12: 31,302,954	T885A	possibly damaging	Het
Lamp5	A	G	2: 136,060,954	T198A	probably benign	Het
Lipi	A	T	16: 75,555,822	L376I	probably benign	Het
Luc7l	T	C	17: 26,254,004	I31T	possibly damaging	Het
Malrd1	A	T	2: 15,845,227	N1219I	unknown	Het
Mcf2l	C	T	8: 12,984,330		probably benign	Het
Mrc1	A	C	2: 14,307,949	N894T	probably damaging	Het
Mrpl35	C	T	6: 71,816,287	A127T	possibly damaging	Het
Myom2	T	A	8: 15,102,589	Y645*	probably null	Het
Naip2	T	C	13: 100,189,136	H88R	probably benign	Het
Noc3l	T	A	19: 38,810,307	K282N	probably damaging	Het
Nwd2	T	C	5: 63,805,898	Y942H	probably damaging	Het
Olfr1123	T	C	2: 87,418,315	V87A	probably benign	Het
Olfr646	T	A	7: 104,107,095	I272N	probably damaging	Het
Osbpl9	C	A	4: 109,073,136	A221S	probably benign	Het
Paip1	T	C	13: 119,430,265	L45S	probably benign	Het
Peak1	T	C	9: 56,206,654	T1668A	probably benign	Het
Pgap3	A	G	11: 98,390,776	F199L	possibly damaging	Het
Ppip5k2	T	C	1: 97,711,819	M1061V	probably benign	Het
Ppp6r3	A	T	19: 3,494,017	W333R	probably damaging	Het
Ptk2b	T	A	14: 66,174,793	N383I	probably benign	Het
Rab21	G	C	10: 115,315,175	R58G	probably benign	Het
Rabif	C	T	1: 134,506,197	T83I	probably damaging	Het
Rdh7	A	T	10: 127,888,561	F18Y	probably benign	Het
Sept5	A	G	16: 18,623,111	M315T	possibly damaging	Het
Slc27a6	A	G	18: 58,582,234	Y303C	probably damaging	Het
Slc7a7	T	A	14: 54,369,836	M495L	probably benign	Het
Sntg1	T	A	1: 8,677,850		probably null	Het
Spred2	T	A	11: 20,001,019	I72N	probably benign	Het
St3gal1	A	T	15: 67,108,237	I271N	possibly damaging	Het
Stox1	A	T	10: 62,659,607	H962Q	probably benign	Het
Supt6	A	T	11: 78,212,838	M1347K	probably damaging	Het
Susd2	G	T	10: 75,639,618	A484D	possibly damaging	Het
Tpbg	C	A	9: 85,844,467	A163E	possibly damaging	Het
Tpx2	T	C	2: 152,882,335	Y344H	probably damaging	Het
Ttf2	A	T	3: 100,962,712	F348L	probably benign	Het
Ttn	A	T	2: 76,833,306	V11675E	unknown	Het
Txnl4b	T	A	8: 109,572,835	Y142*	probably null	Het
Vmn2r18	G	A	5: 151,561,675	R785C	possibly damaging	Het
Zbtb18	T	A	1: 177,448,478	V459E	probably damaging	Het

Other mutations in Tmem260

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00324:Tmem260	APN	14	48486879	missense	probably benign	0.27
IGL00338:Tmem260	APN	14	48477636	missense	probably damaging	0.97
IGL00508:Tmem260	APN	14	48509121	missense	probably damaging	0.99
IGL01308:Tmem260	APN	14	48511958	missense	probably damaging	1.00
IGL01548:Tmem260	APN	14	48480325	missense	possibly damaging	0.60
IGL01952:Tmem260	APN	14	48472476	missense	probably damaging	1.00
IGL03068:Tmem260	APN	14	48486914	splice site	probably benign
IGL03081:Tmem260	APN	14	48496293	missense	probably benign	0.14
R0131:Tmem260	UTSW	14	48483322	nonsense	probably null
R0131:Tmem260	UTSW	14	48483322	nonsense	probably null
R0132:Tmem260	UTSW	14	48483322	nonsense	probably null
R0149:Tmem260	UTSW	14	48452047	missense	possibly damaging	0.88
R0361:Tmem260	UTSW	14	48452047	missense	possibly damaging	0.88
R0396:Tmem260	UTSW	14	48486867	missense	possibly damaging	0.83
R0524:Tmem260	UTSW	14	48472478	missense	probably benign	0.00
R1734:Tmem260	UTSW	14	48509093	missense	probably benign	0.00
R2152:Tmem260	UTSW	14	48477609	missense	possibly damaging	0.62
R2474:Tmem260	UTSW	14	48496324	missense	probably null	0.90
R2928:Tmem260	UTSW	14	48486750	missense	probably damaging	1.00
R2999:Tmem260	UTSW	14	48484989	missense	possibly damaging	0.92
R3030:Tmem260	UTSW	14	48485001	missense	probably damaging	1.00
R4273:Tmem260	UTSW	14	48505304	missense	probably benign	0.11
R4276:Tmem260	UTSW	14	48477636	missense	probably damaging	0.97
R4277:Tmem260	UTSW	14	48477636	missense	probably damaging	0.97
R4278:Tmem260	UTSW	14	48477636	missense	probably damaging	0.97
R4791:Tmem260	UTSW	14	48511994	intron	probably benign
R4792:Tmem260	UTSW	14	48511994	intron	probably benign
R4810:Tmem260	UTSW	14	48472473	missense	probably damaging	0.99
R5189:Tmem260	UTSW	14	48509116	missense	probably benign	0.01
R5280:Tmem260	UTSW	14	48505259	missense	probably benign	0.02
R5289:Tmem260	UTSW	14	48486810	missense	possibly damaging	0.79
R5322:Tmem260	UTSW	14	48486849	missense	probably damaging	0.99
R5491:Tmem260	UTSW	14	48512170	splice site	probably null
R5593:Tmem260	UTSW	14	48474044	missense	probably benign	0.38
R5606:Tmem260	UTSW	14	48484980	missense	probably damaging	1.00
R5824:Tmem260	UTSW	14	48505328	missense	probably damaging	1.00
R5947:Tmem260	UTSW	14	48486801	missense	possibly damaging	0.93
R6587:Tmem260	UTSW	14	48496456	splice site	probably null
R7234:Tmem260	UTSW	14	48505329	nonsense	probably null
R7236:Tmem260	UTSW	14	48509190	splice site	probably null
R7836:Tmem260	UTSW	14	48509062	missense	probably benign	0.04
R8795:Tmem260	UTSW	14	48451913	missense	probably damaging	1.00
R9056:Tmem260	UTSW	14	48480317	missense	probably benign
R9096:Tmem260	UTSW	14	48520346	missense	unknown
R9384:Tmem260	UTSW	14	48486819	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- TTTCTGAAGCAGCTGTGTGCC -3'
(R):5'- AATCGATGCTCAGGTCTCAGC -3'

Sequencing Primer
(F):5'- AAGCAGCTGTGTGCCTCATC -3'
(R):5'- TTATCCTCAGCTCGCATAAACTGGAG -3'

Posted On

2022-03-30