Mutagenetix > Incidental Mutation

Incidental Mutation 'R9323:Zfp612'

706325

Institutional Source

Beutler Lab

Gene Symbol

Zfp612

Ensembl Gene

ENSMUSG00000044676

Gene Name

zinc finger protein 612

Synonyms

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9323 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

110079746-110092741 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 110088740 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 193 (E193G)

Ref Sequence

ENSEMBL: ENSMUSP00000148717 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000058804] [ENSMUST00000165700] [ENSMUST00000212754]

AlphaFold

A0A1D5RMC2

Predicted Effect

probably benign
Transcript: ENSMUST00000058804
AA Change: E154G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000062474
Gene: ENSMUSG00000044676
AA Change: E154G

Domain	Start	End	E-Value	Type
KRAB	11	71	2e-32	SMART
low complexity region	135	146	N/A	INTRINSIC
ZnF_C2H2	225	247	1.45e-2	SMART
ZnF_C2H2	253	275	2.67e-1	SMART
ZnF_C2H2	281	303	1.4e-4	SMART
ZnF_C2H2	309	331	2.91e-2	SMART
ZnF_C2H2	337	359	3.16e-3	SMART
ZnF_C2H2	365	387	4.17e-3	SMART
ZnF_C2H2	393	415	1.82e-3	SMART
ZnF_C2H2	421	443	3.69e-4	SMART
ZnF_C2H2	449	471	3.69e-4	SMART
ZnF_C2H2	477	499	1.58e-3	SMART
ZnF_C2H2	505	527	4.87e-4	SMART
ZnF_C2H2	533	555	1.38e-3	SMART
ZnF_C2H2	561	583	5.06e-2	SMART
ZnF_C2H2	589	611	5.9e-3	SMART
ZnF_C2H2	617	639	9.44e-2	SMART
ZnF_C2H2	645	667	1.03e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000165700

Predicted Effect

probably benign
Transcript: ENSMUST00000212754
AA Change: E193G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1520401A03Rik	A	G	17: 23,718,413	S54P	unknown	Het
5430419D17Rik	T	A	7: 131,226,672	N285K	probably damaging	Het
Abat	G	A	16: 8,602,371	W178*	probably null	Het
Alx1	G	A	10: 103,022,263	R192*	probably null	Het
Apom	A	G	17: 35,131,657	C23R	probably damaging	Het
Asap2	A	G	12: 21,112,147	N35S	probably benign	Het
Atf6	A	T	1: 170,855,113	Y43*	probably null	Het
Bag4	T	C	8: 25,771,333	S127G	possibly damaging	Het
Bag4	G	T	8: 25,785,152	S7*	probably null	Het
Ccdc88b	A	G	19: 6,849,107	L1080P	probably damaging	Het
Cdkl2	A	T	5: 92,020,248	D362E	probably benign	Het
Cep170	A	G	1: 176,758,502	S575P	probably benign	Het
Col4a2	C	T	8: 11,443,413	P1374L	possibly damaging	Het
Cpt2	T	C	4: 107,904,359	H617R	probably benign	Het
Cyp2d22	A	G	15: 82,374,006	S138P	probably damaging	Het
Cyp2j11	T	C	4: 96,307,382	D359G	probably benign	Het
Ddb2	T	C	2: 91,211,992	S419G	possibly damaging	Het
Dmrtc2	A	T	7: 24,872,916	I121F	probably benign	Het
Dus1l	C	T	11: 120,793,898	R149H	probably damaging	Het
Fam214a	C	T	9: 74,976,133		probably benign	Het
Fam35a	A	G	14: 34,259,639	V514A	probably damaging	Het
Fam90a1a	T	A	8: 21,963,624	Y332N	probably benign	Het
Fbxo47	A	T	11: 97,879,428	V46D	probably benign	Het
Grpel1	T	A	5: 36,470,663	V96E	possibly damaging	Het
Ifi207	A	T	1: 173,727,577	N853K	probably damaging	Het
Igkv4-80	C	T	6: 69,016,767	A47T	probably damaging	Het
Il1a	T	A	2: 129,307,906	I25F	probably benign	Het
Ireb2	T	A	9: 54,904,239		probably null	Het
Itpr1	A	G	6: 108,352,018	Y131C	probably damaging	Het
Kmt2a	T	C	9: 44,819,964		probably benign	Het
Lancl1	T	A	1: 67,038,635		probably benign	Het
Lpl	G	A	8: 68,887,544	V64M	possibly damaging	Het
March10	T	C	11: 105,389,755	H568R	probably damaging	Het
Mib1	G	A	18: 10,775,685	V546M	probably damaging	Het
Mss51	A	T	14: 20,484,871	I277N	probably benign	Het
Mybpc1	A	G	10: 88,524,967		probably null	Het
Myct1	A	C	10: 5,604,195	I21L	probably benign	Het
Myo5c	C	A	9: 75,246,249	A139E	probably damaging	Het
Nlrp4e	C	G	7: 23,321,330	A414G	probably benign	Het
Npy2r	A	T	3: 82,540,421	I349N	possibly damaging	Het
Nrap	T	C	19: 56,389,823	I19V	probably benign	Het
Olfr113	A	C	17: 37,575,244	Y60D	probably damaging	Het
Olfr478	C	T	7: 108,032,023	V107I	probably benign	Het
Olfr659	T	C	7: 104,671,013	F104L	possibly damaging	Het
Olfr803	A	T	10: 129,691,960	V27D	probably benign	Het
Olfr930	T	C	9: 38,930,522	V117A	probably benign	Het
Olfr978	T	C	9: 39,994,064	S85P	possibly damaging	Het
Pde6b	A	T	5: 108,403,432	N194I	probably damaging	Het
Polg	TCTGGATGA	TCTGGATGACTGGATGA	7: 79,465,031		probably null	Het
Polg	GA	GACTGGAGCA	7: 79,465,038		probably null	Het
Ptpn9	T	C	9: 57,027,417	M155T	possibly damaging	Het
Ret	A	G	6: 118,182,014	Y146H	probably benign	Het
Sdf2l1	T	C	16: 17,131,634	H116R	probably damaging	Het
Slc37a1	T	C	17: 31,333,669	I316T	probably damaging	Het
Slc4a11	A	C	2: 130,686,910	L473R	possibly damaging	Het
Smg7	A	G	1: 152,856,002	M344T	probably benign	Het
Smg9	T	C	7: 24,415,040	L268P	probably damaging	Het
Tiparp	C	T	3: 65,531,851	T196I	probably benign	Het
Trappc12	A	G	12: 28,692,492		probably null	Het
Trappc9	A	T	15: 72,693,582	N953K	probably benign	Het
Trpa1	A	G	1: 14,898,340	V428A	probably benign	Het
Utp20	T	C	10: 88,747,308	R2728G	probably damaging	Het

Other mutations in Zfp612

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
H8562:Zfp612	UTSW	8	110090038	missense	probably damaging	1.00
R1920:Zfp612	UTSW	8	110088463	missense	probably benign	0.03
R2994:Zfp612	UTSW	8	110089417	missense	probably damaging	1.00
R4281:Zfp612	UTSW	8	110090059	missense	probably damaging	0.98
R4378:Zfp612	UTSW	8	110089051	missense	possibly damaging	0.92
R4492:Zfp612	UTSW	8	110089297	missense	probably damaging	0.99
R4748:Zfp612	UTSW	8	110088672	missense	probably benign	0.37
R4890:Zfp612	UTSW	8	110089944	nonsense	probably null
R5200:Zfp612	UTSW	8	110089900	nonsense	probably null
R5443:Zfp612	UTSW	8	110089595	missense	possibly damaging	0.85
R5864:Zfp612	UTSW	8	110089726	missense	probably damaging	1.00
R6177:Zfp612	UTSW	8	110089974	missense	probably damaging	1.00
R6435:Zfp612	UTSW	8	110089320	missense	probably damaging	0.99
R6601:Zfp612	UTSW	8	110089549	missense	possibly damaging	0.92
R7082:Zfp612	UTSW	8	110089705	missense	probably damaging	1.00
R7083:Zfp612	UTSW	8	110089136	missense	probably damaging	1.00
R8837:Zfp612	UTSW	8	110088971	missense	probably damaging	1.00
R9394:Zfp612	UTSW	8	110084361	missense	probably damaging	1.00
RF007:Zfp612	UTSW	8	110089542	nonsense	probably null
RF008:Zfp612	UTSW	8	110089561	missense	probably damaging	1.00
Z1176:Zfp612	UTSW	8	110088863	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AATCCGGTCAGCAGGAAGTG -3'
(R):5'- GTGGGAACTGTAGCTGAAGC -3'

Sequencing Primer
(F):5'- CGGTCAGCAGGAAGTGAGAGG -3'
(R):5'- GGAACTGTAGCTGAAGCACTTCC -3'

Posted On

2022-04-18