Mutagenetix > Incidental Mutation

Incidental Mutation 'R9325:Fcgr4'

706428

Institutional Source

Beutler Lab

Gene Symbol

Fcgr4

Ensembl Gene

ENSMUSG00000059089

Gene Name

Fc receptor, IgG, low affinity IV

Synonyms

CD16-2, 4833442P21Rik, Fcgr3a, FcgammaRIV

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R9325 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

170846495-170857330 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 170847711 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 103 (V103A)

Ref Sequence

ENSEMBL: ENSMUSP00000077873 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078825]

AlphaFold

A0A0B4J1G0

Predicted Effect

probably damaging
Transcript: ENSMUST00000078825
AA Change: V103A

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000077873
Gene: ENSMUSG00000059089
AA Change: V103A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	31	104	5.79e-9	SMART
IG	112	188	1.44e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (59/60)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit decreased susceptibility to induced arthritis and nephrotoxic nephritis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	A	G	6: 121,646,578 (GRCm39)	D1076G	possibly damaging	Het
Aadacl2fm1	T	A	3: 59,843,862 (GRCm39)	N185K	probably benign	Het
Adamts9	T	C	6: 92,849,279 (GRCm39)	D1060G	probably benign	Het
Ahnak	T	A	19: 8,991,257 (GRCm39)	D4180E	probably benign	Het
Ank2	A	C	3: 126,775,504 (GRCm39)	F42V	probably damaging	Het
Ankib1	A	G	5: 3,822,523 (GRCm39)	I61T	possibly damaging	Het
Arhgap9	T	C	10: 127,161,722 (GRCm39)	S238P	probably damaging	Het
Bpifa3	A	T	2: 153,975,600 (GRCm39)	I57F	probably damaging	Het
Cdh17	A	G	4: 11,810,319 (GRCm39)	Y670C	probably damaging	Het
Ceacam20	C	A	7: 19,720,607 (GRCm39)	R515S	probably benign	Het
Cers5	A	T	15: 99,637,338 (GRCm39)	V274E	probably damaging	Het
Clip1	T	C	5: 123,751,186 (GRCm39)	D865G		Het
Cnih3	G	A	1: 181,181,072 (GRCm39)		probably null	Het
Coro2b	G	T	9: 62,396,609 (GRCm39)	H44N	probably benign	Het
Cuta	A	G	17: 27,158,289 (GRCm39)		probably null	Het
Dchs1	T	C	7: 105,415,402 (GRCm39)	I625V	possibly damaging	Het
Ddx4	A	T	13: 112,736,441 (GRCm39)	F643Y	probably damaging	Het
Dlc1	A	T	8: 37,038,518 (GRCm39)	V1020E	possibly damaging	Het
Dnah1	G	T	14: 30,998,160 (GRCm39)	T2559K	possibly damaging	Het
Eng	C	T	2: 32,561,445 (GRCm39)	A152V	probably damaging	Het
F7	G	T	8: 13,083,430 (GRCm39)	R190L	probably benign	Het
Fer1l4	C	T	2: 155,877,934 (GRCm39)	V1005I	probably damaging	Het
Fmod	A	T	1: 133,968,371 (GRCm39)	H137L	probably damaging	Het
Fndc7	T	C	3: 108,790,834 (GRCm39)	D64G	possibly damaging	Het
Gucy2c	A	T	6: 136,743,992 (GRCm39)	C202*	probably null	Het
Hspg2	C	T	4: 137,265,552 (GRCm39)	R1783W	probably damaging	Het
Hyal6	T	A	6: 24,743,455 (GRCm39)	S384T	probably damaging	Het
Igsf21	T	A	4: 139,794,466 (GRCm39)	I136F	probably damaging	Het
Isl1	A	G	13: 116,436,105 (GRCm39)	S321P	probably damaging	Het
Kif5c	A	G	2: 49,639,378 (GRCm39)	K813R	probably benign	Het
Klk7	A	G	7: 43,461,437 (GRCm39)	E18G	possibly damaging	Het
Lox	T	C	18: 52,661,400 (GRCm39)	M225V	probably benign	Het
Lrrfip2	T	A	9: 110,990,429 (GRCm39)	M50K	possibly damaging	Het
Lrrn2	A	T	1: 132,865,241 (GRCm39)	Q102L	probably damaging	Het
Mcm3	T	C	1: 20,875,562 (GRCm39)	R692G	probably benign	Het
Mmp14	T	C	14: 54,676,248 (GRCm39)	V326A	probably damaging	Het
Muc2	G	A	7: 141,298,559 (GRCm39)	M70I		Het
Nefl	T	C	14: 68,322,460 (GRCm39)		probably null	Het
Nlrp4e	C	G	7: 23,020,755 (GRCm39)	A414G	probably benign	Het
Nup188	T	A	2: 30,212,271 (GRCm39)	L586Q	probably damaging	Het
Or10ag58	G	A	2: 87,265,290 (GRCm39)	G153D	possibly damaging	Het
Or8c13	T	C	9: 38,091,327 (GRCm39)	D264G	probably benign	Het
Pik3c2a	A	T	7: 115,990,558 (GRCm39)	D467E	probably damaging	Het
Polr2g	T	A	19: 8,774,669 (GRCm39)	K71N	probably benign	Het
Pramel31	A	G	4: 144,089,093 (GRCm39)	H137R	probably benign	Het
Prkci	A	G	3: 31,085,333 (GRCm39)	K184R	probably damaging	Het
Prr15	G	A	6: 54,306,137 (GRCm39)		probably benign	Het
Rin2	T	A	2: 145,727,819 (GRCm39)	N896K	probably benign	Het
Ryr3	T	C	2: 112,479,640 (GRCm39)	E4414G	probably damaging	Het
Slc4a4	T	A	5: 89,376,756 (GRCm39)	D994E	probably damaging	Het
Slc6a4	C	A	11: 76,909,999 (GRCm39)	P418Q	probably benign	Het
Smarcc2	T	A	10: 128,324,076 (GRCm39)	M1138K	unknown	Het
Snph	C	T	2: 151,436,208 (GRCm39)	R240Q	probably damaging	Het
Stox2	T	A	8: 47,647,095 (GRCm39)	T122S	probably benign	Het
Tmem131l	T	C	3: 83,817,768 (GRCm39)	D1244G	probably benign	Het
Tmem168	T	C	6: 13,583,253 (GRCm39)	I543V	probably benign	Het
Tyw1	T	C	5: 130,291,762 (GRCm39)	S77P	probably damaging	Het
Usp36	T	G	11: 118,160,031 (GRCm39)	D471A	possibly damaging	Het
Vmn2r102	A	G	17: 19,897,558 (GRCm39)	Y191C	probably damaging	Het
Vmn2r104	T	C	17: 20,268,433 (GRCm39)	Q12R	possibly damaging	Het
Zfp820	A	T	17: 22,038,380 (GRCm39)	I316N	probably damaging	Het
Zscan4-ps3	T	C	7: 11,344,227 (GRCm39)	F62L	probably damaging	Het

Other mutations in Fcgr4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01015:Fcgr4	APN	1	170,853,358 (GRCm39)	missense	possibly damaging	0.72
R0193:Fcgr4	UTSW	1	170,853,329 (GRCm39)	missense	possibly damaging	0.76
R0526:Fcgr4	UTSW	1	170,856,760 (GRCm39)	missense	probably damaging	0.98
R1164:Fcgr4	UTSW	1	170,856,739 (GRCm39)	missense	possibly damaging	0.75
R1544:Fcgr4	UTSW	1	170,847,523 (GRCm39)	missense	probably damaging	1.00
R1716:Fcgr4	UTSW	1	170,847,672 (GRCm39)	missense	probably damaging	1.00
R1905:Fcgr4	UTSW	1	170,856,874 (GRCm39)	missense	probably damaging	0.99
R6012:Fcgr4	UTSW	1	170,853,233 (GRCm39)	missense	possibly damaging	0.80
R6043:Fcgr4	UTSW	1	170,847,699 (GRCm39)	missense	probably damaging	1.00
R6333:Fcgr4	UTSW	1	170,856,838 (GRCm39)	missense	probably damaging	0.97
R7034:Fcgr4	UTSW	1	170,847,657 (GRCm39)	missense	probably benign	0.00
R7036:Fcgr4	UTSW	1	170,847,657 (GRCm39)	missense	probably benign	0.00
R8123:Fcgr4	UTSW	1	170,847,572 (GRCm39)	missense	probably benign
R8791:Fcgr4	UTSW	1	170,847,477 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGTGAACCTAGACCCCAAG -3'
(R):5'- GATACCATCCTGAACGCACTGC -3'

Sequencing Primer
(F):5'- AGACAGCGTGACCCTCAGATG -3'
(R):5'- CTGAACGCACTGCAGGAGTTTG -3'

Posted On

2022-04-18