Mutagenetix > Incidental Mutation

Incidental Mutation 'R9329:Vmn1r189'

706746

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r189

Ensembl Gene

ENSMUSG00000099611

Gene Name

vomeronasal 1 receptor 189

Synonyms

V1rh2

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.371) question?

Stock #

R9329 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

22285897-22286835 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 22286430 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 136 (I136L)

Ref Sequence

ENSEMBL: ENSMUSP00000140924 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000186062] [ENSMUST00000227357] [ENSMUST00000228428]

AlphaFold

Q8K3N3

Predicted Effect

probably benign
Transcript: ENSMUST00000186062
AA Change: I136L

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000140924
Gene: ENSMUSG00000099611
AA Change: I136L

Domain	Start	End	E-Value	Type
Pfam:7tm_1	30	283	1e-7	PFAM
Pfam:V1R	33	297	7.9e-35	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000227357
AA Change: I136L

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

Predicted Effect

probably benign
Transcript: ENSMUST00000228428
AA Change: I136L

PolyPhen 2 Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

99% (73/74)

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700003E16Rik	T	G	6: 83,133,556 (GRCm39)	M1R	probably null	Het
Acap2	T	C	16: 30,946,238 (GRCm39)	D235G	probably damaging	Het
Adgrv1	C	A	13: 81,647,241 (GRCm39)	V3100F	probably damaging	Het
Aff4	A	G	11: 53,288,686 (GRCm39)	T401A	probably damaging	Het
Apcs	T	C	1: 172,722,391 (GRCm39)	T22A	probably benign	Het
Atf7ip2	T	C	16: 10,059,738 (GRCm39)	V426A	possibly damaging	Het
Bach2	A	G	4: 32,562,175 (GRCm39)	D214G	possibly damaging	Het
Baz2a	C	T	10: 127,960,925 (GRCm39)	A1578V	possibly damaging	Het
Camk1d	T	C	2: 5,449,954 (GRCm39)	I76V	probably benign	Het
Ccdc182	A	T	11: 88,185,324 (GRCm39)	M135L	probably benign	Het
Cd47	C	T	16: 49,716,731 (GRCm39)	T201I		Het
Chodl	T	C	16: 78,746,030 (GRCm39)	S290P	possibly damaging	Het
Chst15	A	G	7: 131,868,520 (GRCm39)	L300P	possibly damaging	Het
Ciita	T	C	16: 10,324,571 (GRCm39)	S168P	probably damaging	Het
Cntn2	C	T	1: 132,456,678 (GRCm39)	V123I	probably benign	Het
Cyld	T	A	8: 89,457,348 (GRCm39)	L527Q	probably benign	Het
Dbn1	T	A	13: 55,631,241 (GRCm39)	Y34F	probably damaging	Het
Enc1	C	A	13: 97,383,018 (GRCm39)	Y509*	probably null	Het
Ermard	A	T	17: 15,273,643 (GRCm39)	E409D	probably benign	Het
Extl3	T	C	14: 65,314,765 (GRCm39)	E139G	possibly damaging	Het
Fa2h	A	T	8: 112,082,115 (GRCm39)	V177E	possibly damaging	Het
Flot2	T	C	11: 77,949,772 (GRCm39)	I367T	probably damaging	Het
Fras1	T	C	5: 96,884,813 (GRCm39)	Y2564H	probably damaging	Het
Fxyd3	A	G	7: 30,773,018 (GRCm39)	V10A	probably benign	Het
Gfra3	A	G	18: 34,837,560 (GRCm39)	L146P	probably damaging	Het
Gm49359	T	C	13: 62,602,448 (GRCm39)	N251D	probably benign	Het
Hps6	A	G	19: 45,992,542 (GRCm39)	S160G	probably benign	Het
Hsd17b7	C	A	1: 169,794,875 (GRCm39)	A55S	probably damaging	Het
Hspa12a	T	C	19: 58,789,298 (GRCm39)	D446G	probably benign	Het
Htt	G	A	5: 34,989,957 (GRCm39)	R1163Q	possibly damaging	Het
Ift81	T	C	5: 122,697,833 (GRCm39)		probably null	Het
Ighv5-15	T	G	12: 113,790,395 (GRCm39)	Y75S	probably benign	Het
Kcnd2	T	A	6: 21,725,981 (GRCm39)	H491Q	probably damaging	Het
Lamb3	C	A	1: 193,014,665 (GRCm39)	T608K	probably benign	Het
Lrtm2	T	A	6: 119,297,412 (GRCm39)	K210*	probably null	Het
Med13	G	T	11: 86,189,283 (GRCm39)	P1209T	probably benign	Het
Mtus1	G	A	8: 41,537,239 (GRCm39)	T159I	probably damaging	Het
Myt1l	A	G	12: 29,901,659 (GRCm39)	M805V	unknown	Het
Neb	G	T	2: 52,160,231 (GRCm39)	L2261I	probably benign	Het
Nlrp4c	G	A	7: 6,068,498 (GRCm39)	R133H	probably benign	Het
Nol11	A	T	11: 107,071,765 (GRCm39)	L276Q	probably damaging	Het
Nrap	A	G	19: 56,350,277 (GRCm39)	Y614H	probably damaging	Het
Or13c9	G	A	4: 52,936,211 (GRCm39)	T24M	probably damaging	Het
Or52p1	A	G	7: 104,267,705 (GRCm39)	H273R	probably damaging	Het
Pou2f3	A	G	9: 43,040,224 (GRCm39)	S355P	probably damaging	Het
Ppm1l	C	A	3: 69,460,453 (GRCm39)	N343K	probably damaging	Het
Prh1	A	T	6: 132,548,573 (GRCm39)	I27F	unknown	Het
Prkrip1	A	T	5: 136,223,203 (GRCm39)	L85Q	probably damaging	Het
Pycr3	C	T	15: 75,790,563 (GRCm39)	V99I	probably benign	Het
R3hdm4	T	A	10: 79,749,393 (GRCm39)	N72I	probably damaging	Het
Rab11fip3	G	T	17: 26,231,032 (GRCm39)	H738N	probably benign	Het
Rtl1	G	A	12: 109,556,673 (GRCm39)	A1722V	possibly damaging	Het
Sell	A	G	1: 163,893,122 (GRCm39)	N113D	probably damaging	Het
Smarcal1	T	C	1: 72,665,697 (GRCm39)	S732P	probably damaging	Het
Smurf2	A	T	11: 106,743,424 (GRCm39)	D207E	probably benign	Het
Spata31h1	G	A	10: 82,121,439 (GRCm39)	S3857L	probably benign	Het
Tat	C	T	8: 110,723,510 (GRCm39)	R333C	probably benign	Het
Tmem121b	A	T	6: 120,469,234 (GRCm39)	F494L	probably damaging	Het
Tmem198	C	A	1: 75,456,522 (GRCm39)	P89Q	probably damaging	Het
Tnks2	T	G	19: 36,835,284 (GRCm39)	C2G	probably damaging	Het
Trappc9	T	C	15: 72,673,202 (GRCm39)	E869G	unknown	Het
Trim42	T	A	9: 97,251,584 (GRCm39)	K105M	probably benign	Het
Ttll3	T	C	6: 113,369,635 (GRCm39)	S60P	probably benign	Het
Ttll4	T	A	1: 74,725,121 (GRCm39)	I625N	possibly damaging	Het
Tubgcp5	T	A	7: 55,479,181 (GRCm39)		probably null	Het
Txlnb	A	T	10: 17,682,594 (GRCm39)	Y172F	probably damaging	Het
Ube2e3	A	T	2: 78,744,035 (GRCm39)	D88V	possibly damaging	Het
Vmn2r71	A	G	7: 85,267,950 (GRCm39)	I135V	probably benign	Het
Vmn2r73	T	A	7: 85,519,073 (GRCm39)	R516S	probably benign	Het
Wrn	C	T	8: 33,731,006 (GRCm39)	E1352K	probably benign	Het
Zfp318	T	C	17: 46,722,139 (GRCm39)	S1381P	probably damaging	Het
Zfp747	A	T	7: 126,973,655 (GRCm39)	Y172N	probably damaging	Het

Other mutations in Vmn1r189

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03084:Vmn1r189	APN	13	22,286,008 (GRCm39)	nonsense	probably null
R0437:Vmn1r189	UTSW	13	22,286,231 (GRCm39)	missense	probably damaging	0.99
R1199:Vmn1r189	UTSW	13	22,286,828 (GRCm39)	missense	probably damaging	0.99
R1795:Vmn1r189	UTSW	13	22,286,324 (GRCm39)	missense	probably benign	0.03
R2251:Vmn1r189	UTSW	13	22,286,718 (GRCm39)	missense	probably damaging	1.00
R3751:Vmn1r189	UTSW	13	22,286,382 (GRCm39)	missense	probably benign	0.00
R3824:Vmn1r189	UTSW	13	22,286,382 (GRCm39)	missense	probably benign	0.02
R3825:Vmn1r189	UTSW	13	22,286,382 (GRCm39)	missense	probably benign	0.02
R3856:Vmn1r189	UTSW	13	22,286,439 (GRCm39)	missense	possibly damaging	0.56
R4688:Vmn1r189	UTSW	13	22,286,289 (GRCm39)	missense	probably damaging	0.99
R5665:Vmn1r189	UTSW	13	22,286,336 (GRCm39)	missense	probably damaging	1.00
R5778:Vmn1r189	UTSW	13	22,286,552 (GRCm39)	missense	probably damaging	0.99
R6149:Vmn1r189	UTSW	13	22,286,054 (GRCm39)	missense	probably benign	0.33
R6431:Vmn1r189	UTSW	13	22,286,525 (GRCm39)	missense	probably damaging	0.98
R6660:Vmn1r189	UTSW	13	22,286,066 (GRCm39)	missense	possibly damaging	0.89
R6974:Vmn1r189	UTSW	13	22,286,628 (GRCm39)	missense	probably damaging	1.00
R7051:Vmn1r189	UTSW	13	22,286,285 (GRCm39)	missense	possibly damaging	0.58
R7269:Vmn1r189	UTSW	13	22,286,737 (GRCm39)	missense	probably benign	0.00
R7330:Vmn1r189	UTSW	13	22,286,711 (GRCm39)	missense	possibly damaging	0.95
R7611:Vmn1r189	UTSW	13	22,286,322 (GRCm39)	missense	probably benign	0.02
R7894:Vmn1r189	UTSW	13	22,285,906 (GRCm39)	nonsense	probably null
R8214:Vmn1r189	UTSW	13	22,286,301 (GRCm39)	missense	probably benign	0.01
R8925:Vmn1r189	UTSW	13	22,286,811 (GRCm39)	missense	probably benign	0.01
R8927:Vmn1r189	UTSW	13	22,286,811 (GRCm39)	missense	probably benign	0.01
R9562:Vmn1r189	UTSW	13	22,286,426 (GRCm39)	missense	probably damaging	1.00
R9565:Vmn1r189	UTSW	13	22,286,426 (GRCm39)	missense	probably damaging	1.00
Z1177:Vmn1r189	UTSW	13	22,286,053 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATGGCACCCTGAAACACTG -3'
(R):5'- GACTGCCAAAGACAGTAGCAGC -3'

Sequencing Primer
(F):5'- TGCATCTCTCAGGACCATGAGAG -3'
(R):5'- CTGCCAAAGACAGTAGCAGCTTTTG -3'

Posted On

2022-04-18