Mutagenetix > Incidental Mutation

Incidental Mutation 'R9331:Prl7a2'

706867

Institutional Source

Beutler Lab

Gene Symbol

Prl7a2

Ensembl Gene

ENSMUSG00000046899

Gene Name

prolactin family 7, subfamily a, member 2

Synonyms

Prlpf, PLP-F

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R9331 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

27842567-27852019 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 27849062 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 76 (S76P)

Ref Sequence

ENSEMBL: ENSMUSP00000006660 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006660]

AlphaFold

O54831

Predicted Effect

probably damaging
Transcript: ENSMUST00000006660
AA Change: S76P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000006660
Gene: ENSMUSG00000046899
AA Change: S76P

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	17	244	6.9e-45	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca3	A	G	17: 24,616,324 (GRCm39)	E922G	probably benign	Het
Abca8a	A	G	11: 109,917,154 (GRCm39)	L1615P	probably damaging	Het
Actrt3	T	C	3: 30,652,050 (GRCm39)	D348G	probably damaging	Het
Adam3	A	G	8: 25,177,951 (GRCm39)	M654T	probably benign	Het
Adamts8	A	T	9: 30,862,770 (GRCm39)	H325L	probably damaging	Het
Agpat2	A	G	2: 26,487,318 (GRCm39)	Y72H	probably benign	Het
Ap3m1	A	G	14: 21,095,666 (GRCm39)	Y55H	possibly damaging	Het
Cacna1a	C	T	8: 85,142,446 (GRCm39)	A58V	probably damaging	Het
Cacna1c	C	T	6: 119,084,909 (GRCm39)	V10I		Het
Ccnt1	T	A	15: 98,441,097 (GRCm39)	K724*	probably null	Het
Cd72	T	A	4: 43,454,320 (GRCm39)	T38S	possibly damaging	Het
Crispld1	T	A	1: 17,832,454 (GRCm39)	I480K	probably damaging	Het
Diaph3	G	T	14: 87,378,461 (GRCm39)	Y30*	probably null	Het
Ei24	A	G	9: 36,701,217 (GRCm39)	I34T	possibly damaging	Het
Fdxacb1	G	A	9: 50,681,511 (GRCm39)	S144N	probably damaging	Het
Fgf21	G	T	7: 45,263,614 (GRCm39)	Q155K	probably benign	Het
Gdap1l1	A	T	2: 163,295,664 (GRCm39)	R310S	probably benign	Het
Gm44501	G	A	17: 40,889,620 (GRCm39)	V45I	probably benign	Het
Gne	A	G	4: 44,066,845 (GRCm39)	L56P	probably damaging	Het
Hcn4	T	C	9: 58,767,705 (GRCm39)	S1089P	probably damaging	Het
Hexim1	C	T	11: 103,007,974 (GRCm39)	T76M	probably damaging	Het
Hkdc1	A	T	10: 62,236,114 (GRCm39)	L476*	probably null	Het
Hoxd8	CGCGGCGGCGGCGGCGGC	CGCGGCGGCGGCGGC	2: 74,535,942 (GRCm39)		probably benign	Het
Hyal4	A	T	6: 24,765,866 (GRCm39)	I407L	probably damaging	Het
Ighv5-9-1	T	C	12: 113,699,878 (GRCm39)	Y78C	possibly damaging	Het
Kif20a	T	A	18: 34,762,562 (GRCm39)	C478*	probably null	Het
Klk15	C	T	7: 43,587,790 (GRCm39)	H73Y	possibly damaging	Het
Ltbp2	C	T	12: 84,922,965 (GRCm39)	R34Q	probably benign	Het
Mccc1	A	G	3: 36,014,238 (GRCm39)	V693A	probably damaging	Het
Muc5b	C	A	7: 141,411,475 (GRCm39)	Q1474K	unknown	Het
Nos1	T	C	5: 118,038,589 (GRCm39)	V474A	possibly damaging	Het
Nprl2	G	A	9: 107,421,955 (GRCm39)	V244M	probably damaging	Het
Npy2r	A	G	3: 82,448,068 (GRCm39)	S236P	probably damaging	Het
Or8b9	G	A	9: 37,766,710 (GRCm39)	V199I	probably benign	Het
Or8c10	A	G	9: 38,279,003 (GRCm39)	S44G	probably benign	Het
P2rx7	T	C	5: 122,818,961 (GRCm39)	L461P	probably benign	Het
Pacc1	T	C	1: 191,077,318 (GRCm39)		probably null	Het
Pde12	T	C	14: 26,389,828 (GRCm39)	I294V	probably benign	Het
Plcl1	T	C	1: 55,736,030 (GRCm39)	L457P	possibly damaging	Het
Plekho2	C	A	9: 65,463,866 (GRCm39)	A328S	probably benign	Het
Polg	T	C	7: 79,108,148 (GRCm39)	K556E	probably damaging	Het
Ptar1	T	A	19: 23,671,707 (GRCm39)	C37S	probably benign	Het
Pwwp2b	G	A	7: 138,835,357 (GRCm39)	G266D	probably damaging	Het
Pygm	T	C	19: 6,448,129 (GRCm39)	F812L	probably damaging	Het
Smarcc2	A	T	10: 128,323,310 (GRCm39)	T982S	unknown	Het
Spata31e2	T	C	1: 26,722,790 (GRCm39)	R797G	probably benign	Het
Syne1	C	G	10: 5,073,666 (GRCm39)	V1226L	probably benign	Het
Tmco1	C	T	1: 167,136,132 (GRCm39)		probably benign	Het
Tmub1	T	C	5: 24,650,985 (GRCm39)	T225A	probably damaging	Het
Trmo	A	C	4: 46,387,642 (GRCm39)	C59W	possibly damaging	Het
Ttc6	C	A	12: 57,720,509 (GRCm39)	A925D	probably damaging	Het
Tubb1	A	G	2: 174,297,472 (GRCm39)	E27G	probably damaging	Het
Unkl	G	A	17: 25,450,723 (GRCm39)	C503Y	probably damaging	Het
Usp40	A	G	1: 87,901,828 (GRCm39)	I785T	probably damaging	Het
Vmn2r32	G	A	7: 7,467,402 (GRCm39)	Q709*	probably null	Het
Xaf1	T	C	11: 72,197,470 (GRCm39)	S149P	probably damaging	Het
Ythdc2	T	A	18: 44,970,499 (GRCm39)	V271E	possibly damaging	Het
Zfp386	C	T	12: 116,011,433 (GRCm39)		probably benign	Het
Zfp456	T	A	13: 67,514,389 (GRCm39)	E439V	probably damaging	Het

Other mutations in Prl7a2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01484:Prl7a2	APN	13	27,843,191 (GRCm39)	missense	probably damaging	0.98
IGL02424:Prl7a2	APN	13	27,851,953 (GRCm39)	missense	probably null	0.08
IGL02734:Prl7a2	APN	13	27,843,190 (GRCm39)	missense	probably benign	0.38
IGL02823:Prl7a2	APN	13	27,846,734 (GRCm39)	missense	possibly damaging	0.95
PIT4260001:Prl7a2	UTSW	13	27,843,259 (GRCm39)	nonsense	probably null
R0733:Prl7a2	UTSW	13	27,846,671 (GRCm39)	missense	probably damaging	1.00
R1371:Prl7a2	UTSW	13	27,846,750 (GRCm39)	missense	probably benign	0.01
R1778:Prl7a2	UTSW	13	27,843,254 (GRCm39)	missense	probably damaging	0.98
R1857:Prl7a2	UTSW	13	27,843,163 (GRCm39)	nonsense	probably null
R2063:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2064:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2065:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2067:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2068:Prl7a2	UTSW	13	27,844,870 (GRCm39)	missense	probably damaging	0.98
R2176:Prl7a2	UTSW	13	27,843,089 (GRCm39)	missense	probably benign	0.10
R2213:Prl7a2	UTSW	13	27,849,051 (GRCm39)	missense	probably benign	0.06
R4111:Prl7a2	UTSW	13	27,849,050 (GRCm39)	missense	possibly damaging	0.96
R4459:Prl7a2	UTSW	13	27,849,979 (GRCm39)	missense	probably benign	0.21
R4483:Prl7a2	UTSW	13	27,844,930 (GRCm39)	missense	possibly damaging	0.80
R4722:Prl7a2	UTSW	13	27,844,858 (GRCm39)	missense	probably damaging	1.00
R5360:Prl7a2	UTSW	13	27,843,143 (GRCm39)	missense	probably benign	0.22
R5778:Prl7a2	UTSW	13	27,844,983 (GRCm39)	nonsense	probably null
R6667:Prl7a2	UTSW	13	27,845,024 (GRCm39)	missense	probably benign	0.03
R7107:Prl7a2	UTSW	13	27,843,076 (GRCm39)	missense	possibly damaging	0.89
R7600:Prl7a2	UTSW	13	27,843,264 (GRCm39)	missense	possibly damaging	0.63
R8298:Prl7a2	UTSW	13	27,844,994 (GRCm39)	missense	probably benign	0.00
R8447:Prl7a2	UTSW	13	27,849,941 (GRCm39)	missense	possibly damaging	0.72
R9009:Prl7a2	UTSW	13	27,849,994 (GRCm39)	missense	probably damaging	1.00
R9624:Prl7a2	UTSW	13	27,849,869 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GATTTGATGAAGCTGTCCTCTG -3'
(R):5'- AACAGGAAGGGGTTCTCTGTTTC -3'

Sequencing Primer
(F):5'- AGCTGTCCTCTGCTTTTGAACAG -3'
(R):5'- AGAGCTACATGTTCTCTGTTCAG -3'

Posted On

2022-04-18