Mutagenetix > Incidental Mutation

Incidental Mutation 'R9335:Olfr169'

707104

Institutional Source

Beutler Lab

Gene Symbol

Olfr169

Ensembl Gene

ENSMUSG00000068535

Gene Name

olfactory receptor 169

Synonyms

GA_x54KRFPKG5P-16014972-16014031, MOR273-3P

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R9335 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19564889-19571809 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 19566763 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 40 (T40I)

Ref Sequence

ENSEMBL: ENSMUSP00000149087 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090062] [ENSMUST00000215040] [ENSMUST00000215476] [ENSMUST00000216070]

AlphaFold

Q7TS53

Predicted Effect

probably benign
Transcript: ENSMUST00000090062
AA Change: T40I

PolyPhen 2 Score 0.316 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000087516
Gene: ENSMUSG00000068535
AA Change: T40I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	308	1.3e-45	PFAM
Pfam:7TM_GPCR_Srsx	35	303	2e-6	PFAM
Pfam:7tm_1	41	290	7.4e-29	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215040
AA Change: T40I

PolyPhen 2 Score 0.316 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000215476
AA Change: T40I

PolyPhen 2 Score 0.316 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000216070
AA Change: T40I

PolyPhen 2 Score 0.316 (Sensitivity: 0.90; Specificity: 0.89)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438H23Rik	G	T	16: 91,055,942	T102K	probably damaging	Het
Afm	A	T	5: 90,550,227	D496V	probably damaging	Het
Aldh1l2	C	T	10: 83,506,646	M546I	probably damaging	Het
Apol7c	G	T	15: 77,525,689	N352K	probably benign	Het
Arfgef1	A	T	1: 10,158,011	D1319E	probably damaging	Het
Atl2	A	G	17: 79,852,778	I460T	probably benign	Het
Brms1l	C	A	12: 55,841,646	T73K	possibly damaging	Het
C1rl	A	T	6: 124,505,382	H154L	probably benign	Het
Cacna2d4	T	A	6: 119,302,053	L709Q	probably damaging	Het
Cd101	A	T	3: 101,008,115	V716D	probably benign	Het
Cd177	A	G	7: 24,744,286	S796P	probably benign	Het
D430041D05Rik	A	T	2: 104,248,329	M530K	probably damaging	Het
Dcbld2	A	T	16: 58,451,778	Q399L	probably benign	Het
Dennd4b	T	C	3: 90,268,304	Y138H	probably damaging	Het
Dmxl1	A	G	18: 49,859,120	N311S	probably damaging	Het
Dnaic2	A	G	11: 114,734,663	I107V	probably benign	Het
Dync2h1	A	C	9: 7,112,149	V2480G	possibly damaging	Het
Ect2l	T	A	10: 18,201,284	T24S	probably null	Het
Egfr	C	A	11: 16,870,991	T302K	probably damaging	Het
Eid1	T	G	2: 125,673,658	I156S	possibly damaging	Het
Elmod1	A	T	9: 53,935,832	W21R	probably benign	Het
Enah	G	A	1: 181,921,885	P463L	probably damaging	Het
Epha7	A	G	4: 28,966,529	Y602C	probably benign	Het
Ermap	T	A	4: 119,178,348	Y481F	probably damaging	Het
Fam114a2	G	T	11: 57,506,922	T231N	possibly damaging	Het
Gdap1	A	G	1: 17,161,165	T245A	probably benign	Het
Glmp	T	A	3: 88,328,256	V368D	probably damaging	Het
Gm10203	C	T	6: 149,130,317	A40V	unknown	Het
Gm4553	ACCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCC	ACCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCC	7: 142,165,420		probably benign	Het
Grid1	G	A	14: 35,321,707	D340N	probably damaging	Het
Hao2	C	A	3: 98,883,694	V55L	possibly damaging	Het
Hemgn	A	T	4: 46,394,647	S488T	probably benign	Het
Ighv12-3	C	T	12: 114,366,692	G61E	probably damaging	Het
Itga2b	T	C	11: 102,455,652	E986G	probably damaging	Het
Laptm5	T	G	4: 130,929,528	S167A		Het
Lmtk3	A	T	7: 45,792,741	Y377F	probably damaging	Het
Lrp2	A	G	2: 69,428,639	I4569T	probably benign	Het
Magi2	A	G	5: 20,661,265	N176D		Het
Mcf2l	C	A	8: 13,000,812	H356N	possibly damaging	Het
Mettl4	T	A	17: 94,735,508	Y300F	probably damaging	Het
Naaladl2	T	G	3: 24,413,368	K307N	possibly damaging	Het
Olfr43	T	A	11: 74,207,006	D70V	probably damaging	Het
Olfr610	C	T	7: 103,506,520	R142H	probably benign	Het
Olfr765	C	T	10: 129,046,745	G106E	probably damaging	Het
Phip	T	A	9: 82,932,926	M248L	probably benign	Het
Picalm	A	G	7: 90,176,283	T300A	probably benign	Het
Ppa1	T	G	10: 61,648,783	V19G	possibly damaging	Het
Ppp4r3a	T	C	12: 101,040,754	Y793C	probably damaging	Het
Prlr	A	T	15: 10,325,271	I177F	probably benign	Het
Prodh	C	A	16: 18,076,296	G457V	probably damaging	Het
Prrt3	T	C	6: 113,498,097	K55E	probably benign	Het
Prx	G	T	7: 27,518,071	V805L	probably damaging	Het
Ptk6	T	G	2: 181,202,353	D51A	probably damaging	Het
Rec114	T	A	9: 58,657,758	Q205L	probably null	Het
Rfwd3	T	G	8: 111,279,935	K510Q	possibly damaging	Het
Sdhb	C	T	4: 140,972,939	R117C	probably benign	Het
Serinc2	T	G	4: 130,264,427	T21P	probably benign	Het
Serpinb3a	A	C	1: 107,047,156	F216V	probably damaging	Het
Sertm1	T	C	3: 54,899,508	D32G	probably damaging	Het
Setx	A	G	2: 29,145,951	D816G	probably benign	Het
Sfxn4	T	A	19: 60,851,056	N188I	probably damaging	Het
Slc26a4	T	C	12: 31,525,554	D711G	probably damaging	Het
Sp9	T	C	2: 73,274,277	F392L	probably damaging	Het
Ssbp1	T	C	6: 40,478,021	L138P	probably damaging	Het
Sv2a	G	A	3: 96,185,272	G96E	probably damaging	Het
Tbc1d9	G	T	8: 83,210,531	G81C	possibly damaging	Het
Tmem67	G	A	4: 12,040,640	Q968*	probably null	Het
Tnni2	T	C	7: 142,444,266	S169P	probably damaging	Het
Tpk1	T	C	6: 43,469,150		probably null	Het
Unc13b	T	A	4: 43,216,123	Y141N	possibly damaging	Het
Unc13b	A	T	4: 43,255,551	E1057D	probably damaging	Het
Usf3	G	T	16: 44,221,573	G2139C	probably damaging	Het
Vmn1r36	A	T	6: 66,716,446	Y148*	probably null	Het
Vmn2r42	A	C	7: 8,194,759	M287R	probably damaging	Het
Vmn2r60	A	T	7: 42,194,908	H565L	probably damaging	Het
Zfp518a	G	A	19: 40,912,781	V385I	probably benign	Het
Zfp974	A	T	7: 27,912,051	M83K	probably benign	Het

Other mutations in Olfr169

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01080:Olfr169	APN	16	19566208	missense	probably damaging	1.00
IGL01862:Olfr169	APN	16	19566676	missense	probably damaging	1.00
IGL02064:Olfr169	APN	16	19566548	missense	probably damaging	1.00
IGL03061:Olfr169	APN	16	19566713	missense	possibly damaging	0.87
IGL03136:Olfr169	APN	16	19566353	missense	probably damaging	1.00
R0066:Olfr169	UTSW	16	19566049	missense	probably damaging	1.00
R0243:Olfr169	UTSW	16	19566294	missense	probably damaging	0.97
R0629:Olfr169	UTSW	16	19565980	missense	possibly damaging	0.88
R1644:Olfr169	UTSW	16	19566406	missense	probably benign	0.11
R1943:Olfr169	UTSW	16	19566437	missense	probably benign	0.19
R3016:Olfr169	UTSW	16	19566391	missense	probably damaging	1.00
R4290:Olfr169	UTSW	16	19566244	missense	possibly damaging	0.88
R4689:Olfr169	UTSW	16	19566513	nonsense	probably null
R4791:Olfr169	UTSW	16	19566663	missense	possibly damaging	0.50
R5497:Olfr169	UTSW	16	19566330	missense	probably benign	0.10
R5843:Olfr169	UTSW	16	19566583	missense	probably damaging	1.00
R6106:Olfr169	UTSW	16	19566259	missense	probably damaging	0.99
R6249:Olfr169	UTSW	16	19565975	missense	probably damaging	0.99
R7895:Olfr169	UTSW	16	19566722	nonsense	probably null
R9284:Olfr169	UTSW	16	19566607	missense	probably damaging	1.00
R9364:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68
R9404:Olfr169	UTSW	16	19565981	missense	probably benign	0.01
R9475:Olfr169	UTSW	16	19566520	missense	probably benign	0.09
R9554:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- GCCTGCAAAGGAAATAGTTCTGC -3'
(R):5'- CTATGAGGCACTTGATGATGAATAC -3'

Sequencing Primer
(F):5'- CAAAGGAAATAGTTCTGCTGCCTG -3'
(R):5'- GGCACTTGATGATGAATACAAATTAC -3'

Posted On

2022-04-18